European Review for Medical and Pharmacological Sciences 2020; 24: 12187-12193 Long non-coding RNA PLAC2 suppresses the survival of gastric cancer cells through down-regulating C-Myc C.-C. WU1, Y. YANG2, F.-F. MAO3, W.-X. GUO2, D. WU1 1Department of Gastrointestinal Surgery, Xinghua People’s Hospital, The Affiliated Hospital of Yangzhou University, Taizhou, China 2Department of Hepatic Surgery VI, Third Affiliated Hospital of Second Military Medical University, Shanghai, China 3Department of Tongji University Cancer Center, Tongji University Cancer Center, Shanghai Tenth People’s Hospital, School of Medicine, Tongji University, Shanghai, China Chunchen Wu and Yang Yang contributed equally to this work Abstract. – OBJECTIVE: The aim of this study Introduction was to explore the effects of long non-coding ri- bonucleic acid (lncRNA) placenta-specific pro- Gastric cancer (GC) is the most common tein 2 (PLAC2) on the biological behaviors of highly-invasive malignant tumor in the digestive gastric cancer (GC) cells by regulating the ex- system, whose morbidity and mortality rates are pression of c-Myc gene and its mechanism. 1 PATIENTS AND METHODS: The expression increasing year by year . According to statistics, of PLAC2 in GC tissues and different GC cell there are 35,000 cases of GC deaths in China ev- lines was detected via quantitative Reverse ery year, mostly due to distant metastasis of ad- Transcription-Polymerase Chain Reaction (qRT- vanced GC2. Currently, the specific pathogeny of PCR). The effects of PLAC2 on apoptosis and GC remains unknown, and the median survival cycle, migration, and invasion of GC cells were time of GC patients undergoing convention- detected using flow cytometry, wound healing 3 assay, and transwell assay, respectively. After al drug therapy is less than 15 months . To im- interference in PLAC2 expression, the changes prove the existing diagnosis and treatment of GC, in c-Myc expression were determined through studying the mechanism of GC occurrence and qRT-PCR and Western blotting. metastasis may have a substantial influence on the RESULTS: The expression level of PLAC2 therapeutic strategies in the future, and provide was downregulated in 38 out of 45 cases of GC an effective therapeutic method for GC patients. tissues compared with that in normal gastric Long non-coding ribonucleic acids (lncRNAs) tissues, and it also declined in GC cells. The results of flow cytometry showed that after affect important biological processes of a variety overexpression of PLAC2, the cell cycle was of cells, such as invasion and metastasis of tu- arrested in the G1/G0 phase, and the apoptosis mor cells4,5. HOX tran antisense RNA (HOTAIR) rate was increased. The results of wound heal- is highly expressed in breast tumors, which can ing assay and transwell assay revealed that target specific genomic loci through chroma- both migration and invasion of GC cells were tin repressor proteins, thus promoting metasta- inhibited. After overexpression of PLAC2, the 6 7 mRNA and protein expression levels of c-Myc sis of breast cancer . Fang et al showed that ln- declined. cRNA-p21 associated with hnRNP-K is involved CONCLUSIONS: LncRNA PLAC2 affects the in regulating the transcriptional reaction as a biological behaviors of GC cells by regulating p53-dependent repressor. LncRNA MALAT1 is the expression of c-Myc gene. involved in regulating the cell growth and tumor metastasis, there are many downstream target Key Words: genes, and different activation pathways partici- LncRNA PLAC2, Gastric cancer, Biological behav- 8 iors, c- Myc. pate in the regulation of different behaviors . The above findings suggest that lncRNAs may serve Corresponding Authors: Weixing Guo, MD; e-mail: [email protected] 12187 Duo Wu, MS; e-mail: [email protected] C.-C. Wu, Y. Yang, F.-F. Mao, W.-X. Guo, D. Wu as important regulators of tumorigenesis, but the reagent (Invitrogen, Carlsbad, CA, USA), the RNA regulatory effect of lncRNAs on specific human concentration, and purity were measured using a tumors and their association with tumorigenesis NanoDrop 2000 spectrophotometer, and RNA was have not been fully clarified. reversely transcribed into complementary deoxy- The expression of lncRNA placenta-specif- ribonucleic acid (cDNA) (TaKaRa, Otsu, Shiga, ic protein 2 (PLAC2) is dysregulated in various Japan), followed by cDNA amplification under tumors and it exerts important biological func- the following conditions: pre-denaturation at 95°C tions9,10. Zheng et al11 found that PLAC2 has a for 10 min, denaturation at 95°C for 15 s, and an- downregulated expression in non-small cell lung nealing at 60°C for 32 s for a total of 50 cycles. cancer, which suppresses the tumor growth by Then, the dissolution curve was detected, the Ct adsorbing microRNA-21 (miR-21). Moreover, value of each sample was automatically analyzed PLAC2 induces apoptosis of hepatocellular carci- using the computer system, and the relative expres- noma via upregulating the p53 expression12. How- sion level of lncRNA was calculated using 2-ΔΔCt ever, the expression and function of PLAC2 in GC method. The experiment was repeated for 3 times. have not been reported yet. In the present study, Primers used were shown below: lncRNA PLAC2 it was discovered using in vitro experiments that F 5’-CCATTCCATTCATTTCTCTTTCCTA-3’, PLAC2 was downregulated in GC and inhibited R 5’-GGCGTAGGCGATTGGGGATCG-3’, the formation of malignant phenotype. c-MYC F 5’-ATTTAAGGAGCGGATTTAGC-3’ R 5’-TTTTCGAGTCGAAACACACT-3’, glycer- aldehyde 3-phosphate dehydrogenase (GAPDH) R Patients and Methods 5’-AGCGAGCATCCCCCAAAGTT-3’ F 5’-GGG- CACGAAGGCTCATCATT-3’. Tissue Specimens Tissue specimens were taken from patients un- Plasmid Construction and Transfection dergoing radical gastrectomy in Xinghua People’s The PLAC2 overexpression plasmids were Hospital from January 2016 to December 2017, commercial products synthesized and identified and the patients were definitely diagnosed with by Shanghai Genechem Co., Ltd. (Shanghai, Chi- gastric adenocarcinoma by more than two pathol- na), in which PLAC2 sequences were subcloned ogists and they received no radiotherapy, targeted into pcDNA vectors (pcDNA-PLAC2), with therapy, and immunotherapy before surgery. Af- pcDNA3.1 empty vectors as negative controls. ter excision, tissue specimens were immediately GC cell lines were inoculated into a 6-well plate stored in liquid nitrogen at -180°C for subsequent (5×10 5 cells/well), and they were transfected with experiments. This study was approved by the Eth- pcDNA-PLAC2 and empty vectors using Lipo- ics Committee of Xinghua People’s Hospital and fectamine 2000 (Invitrogen, Carlsbad, CA, USA) patients signed a written informed consent. when 70% of them were fused. The medium was replaced after 6 h, and the cells were collected for Cell Culture qRT-PCR after 48 h. Human GC SGC-7901, AGS, MKN45, and BGC823 cell lines, and normal human gastric Wound Healing Assay mucosal epithelial cell line GES-l were purchased The cells were inoculated into a 24-well plate from the Shanghai Cell Bank of the Chinese Acad- (1×10 4 cells/well). When about 70% of cells were emy of Sciences (Shanghai, China). All cells were fused, they were scratched uniformly along the cultured in the Dulbecco’s Modified Eagle’s Me- diameter of plate using a 20 μL pipette tip perpen- dium (DMEM) or Roswell Park Memorial Insti- dicular to the bottom of plate. The floating cells tute-1640 (RPMI-1640) medium (HyClone, South shed were washed away with phosphate-buffered Logan, UT, USA) containing 10% fetal bovine se- saline (PBS), and fresh medium containing 2% rum (FBS; Gibco, Rockville, MD, USA), strepto- FBS was added. Then, the width of the wound mycin (100 μg/mL), and penicillin (100 U/mL) in was observed and measured at 0 and 36 h, and the an incubator with 5% CO2 and humidity at 37°C. cell migration ability was determined. The assay was repeated for 3 times. Quantitative Reverse Transcription- Polymerase Chain Reaction (qRT-PCR) Transwell Invasion Assay The total RNA was isolated from GC tissue and The transfected cells were cultured till the para-carcinoma normal tissues using the TRIzol logarithmic growth phase, resuspended in the 12188 LncRNA PLAC2 in gastric cancer cells serum-free medium, and added into the upper statistical analyses. The data were expressed as (χ– chamber (1×10 4 cells/well) containing growth ±s), and statistical analysis was performed using medium supplemented by 10% fetal bovine se- Student’s t-test. The association between the ex- rum and growth factor at a normal concentra- pression levels of PLAC2 and c-Myc was detected tion at the bottom. After incubation for 24 h, via Pearson correlation analysis. p<0.05 suggested the cells on the upper side of membrane were the statistically significant difference. removed with Matrigel and the top of membrane was wiped with a cotton swab. Then, the cells on the lower side of membrane were fixed and Results stained with 1% crystal violet dye, followed by photography and observation under an optical Expression of LncRNA PLAC2 microscope. Declined in GC The relative expression level of PLAC2 in 45 Detection of Apoptosis Via Flow cases of GC tissues was detected via qRT-PCR. Cytometry The results revealed that the expression of PLAC2 The transfected cells were collected, washed was downregulated in 38 cases compared with twice with cold PBS, and resuspended in binding that in para-carcinoma tissues (Figure 1A). To buffer, followed by staining using the Annexin study the biological function of PLAC2 in GC V- fluorescein isothiocyanate (FITC) apoptosis cells, its relative expression level in GC cells was assay kit (BD Bioscience, Franklin Lakes, NJ, detected via qRT-PCR. The results showed that USA) at room temperature in the dark for 30 min. the expression of PLAC2 was downregulated The apoptosis rate was measured using a flow cy- (Figure 1B). Then, PLAC2 overexpression vectors tometer (Becton Dickinson, Franklin Lakes, NJ, were synthesized and transiently transfected into USA) within 1 h.