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JOZEF JULIAN BUJARSKI, Professor of Biological Sciences

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JOZEF JULIAN BUJARSKI, Professor of Biological Sciences CURRICULUM VITAE JOZEF JULIAN BUJARSKI Professor of Biological Sciences Northern Illinois University Department of Biological Sciences DeKalb, IL 60115 DATE: January, 2015. EDUCATION M.S., Adam Mickiewicz University, Bioorganic Chemistry, Poznan, Poland, 1972 M.S., Adam Mickiewicz University, Molecular Biology/Biochemistry, Poznan, Poland, 1976 Ph.D., Adam Mickiewicz University, Bioorganic Chemistry, Poznan, Poland, 1978 D.Sc. (Doctor of Science), Institute of Bioorganic Chemistry, Polish Academy of Sciences, May 1998 AREAS OF SPECIALIZATION RNA viruses; Molecular Biology; Biotechnology; Nucleic Acids PROFESSIONAL EXPERIENCE Director of Plant Molecular Biology Center at Northern Illinois University, 2004-present State Professor of the Republic of Poland, July 2003-present Distinguished Research Professor, Northern Illinois University, 2001-present. Professor, Northern Illinois University, Department of Biological Sciences, 1995-present Associate Professor, Northern Illinois University, Department of Biological Sciences, 1991-1994 Assistant Professor, Northern Illinois University, Department of Biological Sciences, 1987-1991 Assistant Scientist, University of Wisconsin-Madison, Biophysics Laboratory, 1985-1987 Research Associate, University of Wisconsin-Madison, Department of Horticulture, 1981-1984 Senior Scientist (Adjunct), Polish Academy of Sciences, Institute of Plant Genetics, Poznan, Poland, 1978-1981 Research Associate, A. Mickiewicz University, Department of Biology, Poznan, Poland, 1976-1978 Research Assistant, A. Mickiewicz University, Department of Chemistry, Poznan, Poland, 1972-1976 Research Assistant, A. Mickiewicz University, Department of Chemistry, Poznan, Poland 1970-1971 HONORS AND AWARDS Ph.D. Graduate Fellowship to Adam Mickiewicz University, Poznan, Poland, 1972-1976. Graduated with honors in the class of bioorganic chemistry, 1972. Tenure at NIU/BIOS, 1991. Presidential Research Professorship Award, Northern Illinois University, 1997. State Professorship from the President of the Republic of Poland, 2003. GRANTS AWARDED 1986-1988, USDA, "Genetic information of bromoviruses and its relationship to host specificity," $90,000. 1988-1990, USDA, "Genetic information of bromoviruses and its relationship to host specificity," JOZEF J. BUJARSKI $90,000. 1988, BRSG (NIU), "Sequencing of BBMV RNA genome," $3,000. 1988, BRSG (NIU), "Molecular biology of bromoviruses," $900 1989, PMBC, "Molecular biology of bromoviruses," $10,000. 1989-1992, NSF(INT), "Construction of bromoviral gene expressing trangenic plants;" collaboration with Switherland, $15,000. 1989, NSF(INSTR), Instrumentation grant to acquire molecular computer graphics system, $40,000 (together with Rogers and Hampel). 1990, BRDC, "Utilization of RET plants to selective amplification of RNA messages," $55,000. 1990-1995, NIH, "Molecular studies on RNA recombination in bromoviruses," $650,000. 1991, BRDC, "Utilization of RET plants to selective amplification of RNA messages," competitive renewal, $12,000. 1992, BRDC, "Utilization of RET plants to selective amplification of RNA messages," competitive renewal, $55,000. 1993, NSF, "The role of replicase in nonhomologous recombination in brome mosaic virus," $350,000. 1994, NATO, "Defective-interfering RNAs in broad bean mottle virus: pathogenicity and accumulation," $5,300 (travel grant to perform a collaborative research with J. Romero, CIT- INIA, Madrid, Spain). 1996-1999, NSF, "The role of replicase in nonhomologous recombination in brome mosaic virus," $450,000. 1996-1998, USDA, "RNA recombination in transgenic plants," $200,000. 1996-1998, NIH, "Homolog9ous recombination in brome mosaic virus," $150,000. 1997, Fulbright Award to spend three months at SCRI, Dundee, Scotland. 2000-2003, NSF, "The role of subgenomic promoter in homologous recombination of brome mosaic bromovirus," $400,000 2003-2008, NSF, “Mechanism in vitro of RNA recombination in brome mosaic bromovirus”. $450,000. 2006-2008, NIH, “Role of RNA interference in genetic recombination of brome mosaic bromovirus”. $150,000. 209-2012, NSF “Role of Subgenomic RNAs in Genetic Recombination of Brome Mosaic Bromovirus”. $495,000. 2 JOZEF J. BUJARSKI PATENTS 1988, "Generation of RNA transcripts containing defined 3' end sequences by autolytic cleavage of larger RNA molecule and vector for use therefore." pending. 1997, "Targeting and enhancing RNA-RNA recombination." A notice of allowance received. 1998, "Method for high-frequency homologous RNA recombination." Pending 1998, "Method for inhibition of nonhomologous genetic RNA recombination." Pending 1999, "Targeting and enhancing of RNA-RNA recombination with antisense sequencing." Awarded. 2010. “Method of expression and purification of RNA dependent RNA polymerase from brome mosaic virus”. US Patent Office. Pending. MEMBERSHIP IN PROFESSIONAL SOCIETIES American Society for Microbiology International Society for Plant-Microbe Interactions American Society for Virology The American Phytopathological Society The RNA Society Polish Biochemical Society PUBLICATIONS Book Chapters 1. Bujarski, J.J. and I. Wiatroszak. 1980. Infection-related proteins in pea leaves infected with bean yellow mosaic virus. In J. Augustyniak, (ed.), Biological implications of protein-nucleic acids interactions. Elsevier/North Holland Biomedical Press, Amsterdam, New York, Oxford, pp. 298-299. 2. Hall, T.C., W.A. Miller, and J.J. Bujarski. 1983. The properties of brome mosaic virus replicase isolated from barley leaves. In Robertson, H.D., Howell, S.H., Zaitlin, M. and Malmberg, R.L. (eds.), Current Communications in Plant Molecular Biology: Plant Infectious Agents-viruses, Viroids and Satellites. Cold Spring Harbor Laboratory, pp. 117-119. 3. Ahlquist, P., French, R., and Bujarski, J.J. 1987. Molecular studies of brome mosaic virus using infectious transcripts from cloned cDNA. Advances in Virus Res., 32:251-242. 4. Bujarski, J.J., and Miller, W.A. 1992. Application of in vitro transcription to studies on small spherical RNA viruses. A review chapter in CRC series. Plant Genetic Engineering with Small Spherical RNA Viruses. 5. Bujarski, J.J. and Nagy, P. 1994. RNA-RNA recombination in plant RNA viruses. In J. Paszkowski (ed.), Homologous Recombination in Plants. Kluwer Academic Publishers, in press. 3 JOZEF J. BUJARSKI 6. Simon, A., and Bujarski, J.J. 1994. RNA-RNA recombination and evolution in virus infected plants. Annual Review of Phytopathology 32:337-362. 7. Bujarski, J.J. 1998. Bromovirus isolation and RNA extraction. In G.D. Foster and S.C. Taylor (eds.), Plant Virology Protocols: From virus isolation to transgenic resistance. Humana Press. 8. Bujarski, J. 1999. Recombination in viruses. In R.G. Webster and A. Granoff (eds.), Encyclopaedia of Virology, Second Edition. 9. Bujarski, J. 1999. Molecular basis of genetic variability in plant RNA viruss. In C.L. Mandahar (ed.), Molecular Biology of Plant Viruses, pp. 121-141. Kluwer Acad. Publ. 10. Roosinck, M.J., J. Bujarski, S.W. Ding, R. Hajimorad, K. Hanada, S. Scott, and M. Tousignant. 1999. Family bromoviridae, p. 923-935. In M.H.V. vanRegenmortel, C.M. Fauquet, and D.H.L. Bishop (ed), Virus taxonomy-seventh report of the International Committee on Taxonomy of Viruses. Academic Press, San Diego. 11. Bujarski, J., 2007. Bromoviruses (Bromoviridae). In R.G. Webster and A. Granoff (eds.), Encyclopaedia of Virology, Third Edition. 12. Bujarski, J. 2007. Recombination in viruses. In R.G. Webster and A. Granoff (eds.), Encyclopaedia of Virology, Third Edition. Review articles 1. Ahlquist P, French R, Bujarski JJ.. 1987. Molecular studies of brome mosaic virus using infectious transcripts from cloned cDNA. Adv Virus Res. 1987;32:215-42. Review. 2. Bujarski, J.J., Nagy, P.D., and Flasinski, S. 1994. Molecular studies of genetic RNA-RNA recombination in brome mosaic virus. Adv. Vir. Res. 43, 275-302. 3. Figlerowicz M, Bujarski JJ. 1997. [Genetic recombination (+) RNA viruses]. Postepy Biochem. 1997;43(4):257-66. Review. 4. Figlerowicz M, Bujarski JJ. 1998. RNA recombination in brome mosaic virus, a model plus strand RNA virus. Acta Biochim Pol. 45(4):847-68. Review. 5. Sztuba, J., Bujarski J.J. 2008. Insights to Bromoviridae single-cell reproduction cycle: lessons from Experiments in Protoplast Systems. J. of Virology. 82, 10330-40. Review. 6. Sztuba-Solińska, J., Stollar, V. and Bujarski J.J. 2011. Subgenomic messenger RNAs: mastering regulation of the positive strand RNA virus life cycle. Virology. Apr 10; 412(2):245- 55. 7. Sztuba-Solinska, J. Urbanowicz, A., Figlerowicz M. and Bujarski J.J. 2011. RNA-RNA recombination and plant virus replication and evolution. Invited review to Annual Review of Phytopathology. Sep 8; 49:415-43. 8. Bujarski JJ. 2013. Genetic recombination in plant-infecting messenger-sense RNA viruses: overview and research perspectives. Front Plant Sci. 4:68. 4 JOZEF J. BUJARSKI Journal Research Articles 1. Paszyc, S. and J.J. Bujarski. 1975. The PMR conformational studies of thiouridines in nonaqueous solutions. Bull. Pol. Acad. Sci. Ser. Chem. 23:477-482. 2. Paszyc, S. and J.J. Bujarski. 1977. Photochemical reactions of sulphur uridine and uracil analogues in aqueous solution. Bull. Pol. Acad. Sci. Ser. Chem. 25:1-9. 3. Bujarski, J.J. and J. Augustyniak. 1977. Large-scale preparations of oligonucleotides from commercial sodium ribonucleate. Annals Pol. Chem. Soc. 51:2273-2276. 4. Bujarski, J.J. 1977. Thionucleotides as tools for tRNA studies. Postepy Biochemii (Progress in Biochemistry) 23: review in Polish. 5. Bujarski, J.J. 1980. Molecular mechanisms
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