DOCSLIB.ORG

(12) Patent Application Publication (10) Pub. No.: US 2007/0298490 A1 Sweigard Et Al

Total Page：16

File Type：pdf, Size：1020Kb

Download full-text PDF Read full-text
(12) Patent Application Publication (10) Pub. No.: US 2007/0298490 A1 Sweigard Et Al US 20070298490A1 (19) United States (12) Patent Application Publication (10) Pub. No.: US 2007/0298490 A1 Sweigard et al. (43) Pub. Date: Dec. 27, 2007 (54) THERMOPHILIC HYDROPHOBIN Related U.S. Application Data PROTEINS AND APPLICATIONS FOR SURFACE MODIFICATION (62) Division of application No. 10/920,876, filed on Aug. 18, 2004, now Pat. No. 7,241,734. (76) Inventors: James A. Sweigard, Elkton, MD (US); Publication Classification Barry Stieglitz, Wynnewood, PA (US) (51) Int. C. C07K I4/00 (2006.01) C7H 2L/04 (2006.01) Correspondence Address: CI2N IS/63 (2006.01) E I DUPONT DE NEMOURS AND (52) U.S. Cl. ...................... 435/320.1; 530/350; 530/371; COMPANY 536/24.3 LEGAL PATENT RECORDS CENTER BARLEY MILL PLAZA 25/1128 (57) ABSTRACT 4417 LANCASTER PIKE WILMINGTON, DE 19805 (US) The present invention relates to a thermophilic hydrophobin, TT1, or a protein or polypeptide Substantially similar thereto, derived from the thermophilic fungus Talaromyces (21) Appl. No.: 11/788,351 thermophilus. The invention further relates to a polynucle otide encoding Such hydrophobin, as well as to materials (22) Filed: Apr. 19, 2007 coated with such hydrophobin. 65 60 55 -0- 3 Hr - - - - - 6 Hr WCA 50 45 - - -A - 16 Hr 40 35 30 25°C 40°C 60°C 80°C Temperoture Patent Application Publication Dec. 27, 2007 Sheet 1 of 3 US 2007/0298490 A1 II:ONTIÒGISITISOTV??INTOIIGNssav?N 305?vºvoaALAGOOAJAH?ãOhashvixos?ownariON™OOTTODITIOvIGÐIVI?LidovxsAAN55vaoNo ?Ô55VIHOX5?ÒNIISSIOTTIGANTIS5xdriology |013 Patent Application Publication Dec. 27, 2007 Sheet 3 of 3 US 2007/0298490 A1 -IH9-º- -IH9--+---- uH9||—-v---- •07009300.089009J 9un?DuÐGUÐ| 9."9|- US 2007/0298490 A1 Dec. 27, 2007 THERMOPHILIC HYDROPHOBIN PROTEINS AND hydrophilic properties, allow them to interact with a wide APPLICATIONS FOR SURFACE MODIFICATION range of material Surfaces. In general, upon contact with either hydrophobic or hydrophilic surfaces, hydrophobin FIELD OF THE INVENTION monomers self-assemble to form a film covering the Surface. A consequence of hydrophobin film formation is a change in 0001. This invention is in the field of biotechnology. surface wettability. Thus on hydrophobic surfaces such as More specifically, this invention pertains to a thermophilic Teflon R film, a hydrophobin coating can increase hydrophi hydrophobin protein and the encoding nucleic acid frag licity as measured by a decrease in the water contact angle. ment, as well as its use as a Surface coating of materials to Conversely, on hydrophilic Surfaces hydrophobin coatings provide beneficial properties. can decrease wettability, measured by an increase in the water contact angle. Particularly Class I hydrophobin SC3 BACKGROUND OF THE INVENTION from the fungus Schizophyllum commune and Class II hydrophobins HFBI and HFBII from Trichoderma reesei 0002 Hydrophobins are small, secreted cysteine-rich have been used in coating studies. HFBI and HFBII formed amphipathic proteins found in fungi. Mature hydrophobin weakly ordered and highly crystalline coatings, respectively, proteins, with secretion signal peptides removed, are gen on water Surfaces (Sermaa, R. et al. Appl. Crystallography erally small proteins that are around 100 amino acids in length. Hydrophobins are characterized by having a pattern 36:499-502). Properties of different hydrophobins, even of 8 highly conserved cysteines that are positioned within among hydrophobins within the same Class, can be quite the mature protein in a 1-2-1-1-2-1 pattern (dashes represent variable in different coating situations. variable numbers of amino acids). Pairs of these 8 cysteines 0005 The novel properties of hydrophobins, including form disulfide bonds, resulting in 4 disulfide bonds that are the ability to self-assemble at interfaces in aqueous Solution necessary for the proper folding of the protein. Though the and under mild conditions, immediately suggest potential 8 cysteines are highly conserved, different hydrophobins are applications for these surface active proteins. Some potential quite variable in their amino acid sequences, some having as applications presented in the literature include: little as 11 % or less sequence identity (Wessels, J. G. H. 0006 use in tissue engineering, particularly in coating of 1994 Annu. Rev. Phytopathol. 32:413-37). Currently, over unnatural Surfaces with a natural protein to increase the 50 hydrophobin sequences from a range of fungal species unnatural Surface biocompatability, e.g., medical implants are known, and are available, for example, through Gen Bank and the National Center for Biotechnology Informa and surgical instruments (Wessels, J. G. H. 1997 Adv. tion (NCBI; Bethesda, Md.). Many fungal species have Microbiol. Physiol. 38:1-45). several reported hydrophobins. Based on differences in 0007 use in drug delivery, especially the delivery of protein primary structure, hydrophobins can be grouped into hydrophilic drugs. Drug oil vesicles coated with a hydro two distinct groups, Class I and Class II. phobin would permit attachment of targeting antibodies to 0003 Genetic studies have implicated hydrophobins as the outside of these vesicles (Wessels, J. G. H. 1997 Adv. playing roles in providing fungal Surface properties. Some Microbiol. Physiol. 38:1-45). hydrophobins, belonging to Class I, are known to form 0008 use in the formation of stable foams in food manu rodlet protein layers found on fungal Surfaces. Rodlet layers facturing and as a natural Surface-active agent in hair prod are extremely hydrophobic and are responsible for generat ucts (Wessels, J. G. H. 1997 Adv. Microbiol. Physiol. 38:1- ing fungal Surface hydrophobicity. Thus all fungal Surfaces 45, Kershaw, M. J. J. Talbot. 1998 Fung. Genet. Biol. 29: including aerial hyphae, fruiting bodies and vegetative 18-33). spores, e.g., conidia, are coated with the hydrophobic domain of self-assembled hydrophobins exposed to the 0009 use to pattern different molecules on a surface with environment and the hydrophilic domain attached or inter nanometer accuracy (Scholtmeijer, K., et al. 2001 Appl. acting with the hydrophilic cell wall. Thus some roles of Microbiol. Biotechnol. 56: 1-8). hydrophobins in nature are to allow fungal hyphae to escape 0010) use to bind or immobilize factors on a hydro into the air from its aqueous environment and search for new phobic support, Such as yeast cells expressing a hydro food sources and Surfaces, to line the internal air spaces in phobin in its cell wall (Nakari-Setala, T., et al. 2002 Schizophyllum commune fruiting bodies in order to prevent Appl. Environ. Microbiol. 68:3385-3391), and several flooding by water of these air channels, and to promote commercially available lipases (Palomo, J. M., et al. interactions between the fungus and the plant during patho 2003 Biomacromol. 4:204-210). genicity, either acting as elicitors of plant defense responses or as stealth factors protecting the invading fungus from 0011 use to develop novel diagnostic sensors through detection and rejection by the plant (Whiteford, J. R. P. D. hydrophobin self-assembly on electrode surfaces (Bilewicz, Spanu 2002 Molec. Plant Pathol. 3: 391-400). R., et al. 2002 J. Phys. Chem. B. 105: 9772-9777). 0004) The best characterized Class I hydrophobin, SC3, 0012 Hydrophobins used in previous studies have gen which is obtained from the fungus Schizophyllum commune, erally been isolated from the natural fungus in which the has been used to study hydrophobin assembly on Surfaces hydrophobin was identified. However, the amounts of pro and the resulting changes in Surface properties. Two Class II tein that can be obtained using this method of preparation are hydrophobins, HFBI and HFBII, isolated from Trichoderma inadequate for commercial applications. A Successful reesei, have also been studied. These hydrophobins have recombinant DNA technology method would be required to been found to be useful as surface coatings to alter the Supply adequate amounts of hydrophobin proteins for com properties of the coated Surfaces. The amphipathic proper mercial uses. An example of recombinant hydrophobin ties of hydrophobins, meaning having both hydrophobic and expression is in WO 00/058342, where the endogenous US 2007/0298490 A1 Dec. 27, 2007 HFBI hydrophobin was overexpressed in Trichoderma 0019. In a third embodiment, the present invention con reesei. Using a recombinant DNA technology method also cerns a recombinant DNA construct comprising any of the allows the creation of hydrophobin variants that may pro isolated polynucleotides of the present invention operably vide Superior properties for use in specific applications. linked to a secretion signal peptide coding sequence and at 0013 Methods of treating surfaces with hydrophobins to least one regulatory sequence. provide a stable coating have been described. In 0020. In a fourth embodiment, the present invention US2003.0134042, Teflon(R) materials were incubated in an concerns an isolated thermophillic hydrophobin polypep SC3 hydrophobin solution at 25° C. and then a heat treat tide, wherein the polypeptide has an amino acid sequence of ment was applied. Aheat treatment of 63°C. or 70° C. in the at least 80%, 85%, 90%, or 95% identity, based on the presence of different detergents at a concentration of 0.1% Clustal W method of alignment, when compared to SEQID was required to obtain strong binding of the hydrophobin to NO:1. The polypeptide most preferably comprises SEQ ID the Teflon R surface. Heat treatments at lower temperatures, NO:1. but above 30°C., in the presence of detergent were partially effective in transitioning the SC3 hydrdphobin to a beta 0021. In a fifth embodiment, the present invention sheet state deemed necessary for strong binding to hydro includes a composite structure comprising a polymer mate phobic Surfaces. rial having a thermophilic hydrophobin coating. 0014) Another method of treating surfaces requires pre 0022. In a sixth embodiment, the present invention pro treatment of the hydrophobin prior to application. In vides a method of treating the surface of an object with a US200301 13454 the disulfide bonds of the SC3 hydro hydrophobin that is performed at a temperature in the range phobin were disrupted by treating with sulphite to add sulfite of greater than 25° C.
Load more

Recommended publications
  • DUPONT DATA BOOK SCIENCE-BASED SOLUTIONS Dupont Investor Relations Contents 1 Dupont Overview
    DUPONT DATA BOOK SCIENCE-BASED SOLUTIONS DuPont Investor Relations Contents 1 DuPont Overview 2 Corporate Financial Data Consolidated Income Statements Greg Friedman Tim Johnson Jennifer Driscoll Consolidated Balance Sheets Vice President Director Director Consolidated Statements of Cash Flows (302) 999-5504 (515) 535-2177 (302) 999-5510 6 DuPont Science & Technology 8 Business Segments Agriculture Electronics & Communications Industrial Biosciences Nutrition & Health Performance Materials Ann Giancristoforo Pat Esham Manager Specialist Safety & Protection (302) 999-5511 (302) 999-5513 20 Corporate Financial Data Segment Information The DuPont Data Book has been prepared to assist financial analysts, portfolio managers and others in Selected Additional Data understanding and evaluating the company. This book presents graphics, tabular and other statistical data about the consolidated company and its business segments. Inside Back Cover Forward-Looking Statements Board of Directors and This Data Book contains forward-looking statements which may be identified by their use of words like “plans,” “expects,” “will,” “believes,” “intends,” “estimates,” “anticipates” or other words of similar meaning. All DuPont Senior Leadership statements that address expectations or projections about the future, including statements about the company’s strategy for growth, product development, regulatory approval, market position, anticipated benefits of recent acquisitions, timing of anticipated benefits from restructuring actions, outcome of contingencies, such as litigation and environmental matters, expenditures and financial results, are forward looking statements. Forward-looking statements are not guarantees of future performance and are based on certain assumptions and expectations of future events which may not be realized. Forward-looking statements also involve risks and uncertainties, many of which are beyond the company’s control.
    [Show full text]
  • Iso 14001:2015
    Certificate of Approval This is to certify that the Management System of: E. I. du Pont de Nemours and Company 974 Centre Road, Wilmington, DE, 19805, United States has been approved by LRQA to the following standards: ISO 14001:2015 Chris Koci Issued By: Lloyd's Register Quality Assurance, Inc. This certificate is valid only in association with the certificate schedule bearing the same number on which the locations applicable to this approval are listed. Current Issue Date: 21 January 2018 Original Approvals: Expiry Date: 20 January 2021 ISO 14001 – 21 January 2009 Certificate Identity Number: 10052905 Approval Number(s): ISO 14001 – 0011717 The scope of this approval is applicable to: Manufacture of Science-Based Products for Agriculture, Nutrition, Electronics, Communications, Safety and Protection, Home and Construction, Transportation and Apparel Markets. Lloyd's Register Group Limited, its affiliates and subsidiaries, including Lloyd's Register Quality Assurance Limited (LRQA), and their respective officers, employees or agents are, individually and collectively, referred to in this clause as 'Lloyd's Register'. Lloyd's Register assumes no responsibility and shall not be liable to any person for any loss, damage or expense caused by reliance on the information or advice in this document or howsoever provided, unless that person has signed a contract with the relevant Lloyd's Register entity for the provision of this information or advice and in that case any responsibility or liability is exclusively on the terms and conditions set out in that contract. Issued By: Lloyd's Register Quality Assurance, Inc., 1330 Enclave Parkway, Suite 200, Houston, Texas 77077, United States Page 1 of 15 Certificate Schedule Certificate Identity Number: 10052905 Location Activities Global Headquarters ISO 14001:2015 974 Centre Road, Wilmington, DE, 19805, Headquarters Activities in Support of the Global United States Manufacturing Sites and EMS Oversight.
    [Show full text]
  • 2 0 0 1 a N N U a L R E P O
    2001 ANNUAL REPORT DuPont at 200 In 2002, DuPont celebrates its 200th anniversary. The company that began as a small, family firm on the banks of Delaware’s Brandywine River is today a global enterprise operating in 70 countries around the world. From a manufacturer of one main product – black powder for guns and blasting – DuPont grew through a remarkable series of scientific leaps into a supplier of some of the world’s most advanced materials, services and technologies. Much of what we take for granted in the look, feel, and utility of modern life was brought to the marketplace as a result of DuPont discoveries, the genius of DuPont scientists and engineers, and the hard work of DuPont employees in plants and offices, year in and year out. Along the way, there have been some exceptional constants. The company’s core values of safety, health and the environment, ethics, and respect for people have evolved to meet the challenges and opportunities of each era, but as they are lived today they would be easily recognizable to our founder. The central role of science as the means for gaining competitive advantage and creating value for customers and shareholders has been consistent. It would be familiar to any employee plucked at random from any decade of the company’s existence. Yet nothing has contributed more to the success of DuPont than its ability to transform itself in order to grow. Whether moving into high explosives in the latter 19th century, into chemicals and polymers in the 20th century, or into biotechnology and other integrated sciences today, DuPont has always embraced change as a means to grow.
    [Show full text]
  • Candidats Potentiels
    Polymères possédant un bon potentiel afin de répondre à l’application de RDDC - Valcartier Contrat: W7701 – 4501411762 Titre: Sélection de polymères Dr. AutoritéFrédéric Byette technique: Emmanuela Diaz, Scientifique de la Défense Prof. Christian Pellerin Université de Montréal, Juillet 2016 DRDC-RDDC-2017-C020 Table des matières Fluorinated ethylene propylene copolymer / FEP (p.3) Perfluoroalkoxy alkane / PFA (p.6) Poly(ethene-co-tetrafluoroethylene) / ETFE (p.9) Poly(tetrafluoroethylene) / PTFE (p.12) Poly(vinylidene fluoride) / PVdF (p.15) Polybutylene terephthalate / PBT (p.17) Polyethylene terephthalate / PET (p.20) Polytrimethylene terephthalate / PTT (p.25) Polyphenylene sulfide / PPS (p.27) Polycarbonate / PC (p.29) Polyoxymethylene / POM (p.32) Polypropylene isotactique / iPP (p.34) Polycaprolactone / PCL (p.37) Polypivalolactone / PPVL (p.38) Nylon 6 (p.40) Nylon 6,6 (p.42) Poly para aramid (p.44) 2 &! )+#2$-'*!&,, 1$&(*'(1$&'('$1%* %#&%&# #,'% 2 #2 '+,$'&<$'& G %'-*+E-('&,H #$%6 #!2+%!@*!+,$$!& %%,%#4=:E%?:E2-!G*6+!+,&, "-+)-B3@ORMY? !%!,'(6*,!'&&$$XNRM@OMMY<%X OSMYH #,#% 2&XN?PQQ ,$$%%*5&%*5&%,2-! $$#, 5%,#-%8;<::7B::4;6;<3?7B3=F92-! !%#42 Indice de Bande Coefficient réfraction (cm-1) d'absorption FEP 1.344 1147 0.535 P Spectres polarisés réflexion spéculaire (indice d’absorption k après transformation de Kramers-Kronig): Ratio d’étirement approximatif = 350% Spectres de réflectance avant et après incubation 24 h à T = 50°C suite à l’étirement: 4 Estimation du DOLP: Bande (cm-1) DOLP FEP 1147 -0.57 Notes et commentaires: Produit similaire au Teflon PTFE, cependant plus souple et donc plus facile à étirer. Très similaire au PFA. Le DOLP est peu affecté suite à l’incubation 24 h à 50°C.
    [Show full text]
  • 25Th Symposium on Biotechnology for Fuels and Chemicals Office of the Biomass Program, U.S
    Sponsors 25th Symposium on Biotechnology for Fuels and Chemicals for 25th Symposium on Biotechnology Office of the Biomass Program, U.S. Department of Energy U. S. Department of Agriculture (USDA) ARS National Renewable Energy Laboratory (NREL) Oak Ridge National Laboratory (ORNL) Argonne National Laboratory (ANL) Idaho National Engineering and Environmental Laboratory (INEEL) Pacific Northwest National Laboratory (PNNL) Alltech Archer Daniels Midland BBI International Biotechnology Industry Organization (BIO) Breckenridge Brewery Cargill, Inc. Cargill-Dow LLC Coors Brewing Company Corn Refiners Association E.I. du Pont de Nemours & Company, Inc. Genencor Iogen Corporation Katzen International Natural Resources, Canada Novozymes Biotech Procter & Gamble Syngenta Tate & Lyle Tembec Industries Prepared by National Renewable Energy Laboratory Golden, Colorado 80041 www.nrel.gov/biotech_symposium/ NREL/BK-510-33708 Printed with a renewable-source ink on paper containing at least 50% wastepaper, including 20% postconsumer waste Organizing Committee Members: Mark Finkelstein, Conference Chairman Welcome National Renewable Energy Laboratory Golden, Colorado from the Organizing Committee Brian Davison, Conference Co-chairman Oak Ridge, National Laboratory Improving the economics of producing fuels and chemicals is vital to many industrial sectors. Oak Ridge, Tennessee We have designed the program for the 25th Symposium on Biotechnology for Fuels and William Apel Chemicals to deliver the latest research breakthroughs and results in biotechnology Idaho National Engineering that stimulate such improvements. Whether you represent the industrial, academic, or and Environmental Laboratory government sector, we invite you to join us and participate in this exciting exchange of Idaho Falls, Idaho information and ideas. You will find valuable opportunities for productive interactions with Doug Cameron your colleagues, both from a national and international perspective.
    [Show full text]
  • Global Environmental Conference -Gpec 2005 Creating Sustainability for the Environment
    GPEC 2005 Paper Abstract #14: Title: DuPont Sorona® Polymer: A New Sustainable Platform for Growth Author(s): Joseph Kurian, Randy Milby, Ray Miller, Bio-Based Materials, DuPont Company, Experimental Station E402/4311 Wilmington, Delaware 19880, USA E-mail: [email protected] Phone: 302-695-7220 ABSTRACT Responding to environmental, sustainability, business and market needs, DuPont has commercialized a new polymer platform, Sorona®, based on 1,3-propanediol. Sorona® polymer can be shaped into fibers and other articles to offer a unique combination of properties and value to customers. Retrofittable Sorona® polymerization and process technology enables manufacturers of apparel and specialty engineering resins to use their existing assets to make new, higher-value products to meet customer needs. DuPont Tate & Lyle Bioproducts, LLC, an equally owned company between DuPont and Tate & Lyle, recently announced plans to create products from renewable resources for numerous applications including clothing, interiors, engineering polymers and textile fibers. The company is constructing its initial commercial manufacturing plant in Loudon, TN., with startup scheduled for 2006. Because corn is a renewable resource, and can be produced anywhere in the world, PDO becomes less expensive to manufacture. Demand for Sorona® polymer is growing in fibers, engineering thermoplastics, films, and several other markets. DuPont has licensed Sorona® fiber technology to a number of manufacturers for applications development. This presentation will highlight Sorona® polymer work focused toward engineering thermoplastics. From corn to polymer to future, Sorona® offers a multitude of advantages. Learn all about the advantages Sorona® offers by visiting http://www.dupont.com/sorona GLOBAL ENVIRONMENTAL CONFERENCE -GPEC 2005 CREATING SUSTAINABILITY FOR THE ENVIRONMENT Presentation Title DuPont Sorona® Polymer: A New Sustainable Platform for Growth Joseph V.
    [Show full text]
  • 2017 Sustainability Report 2 2017 Sustainability Report 3
    2017 SUSTAINABILITY REPORT 2 2017 SUSTAINABILITY REPORT 3 We’re living today in a rapidly changing world. Globalization and technology are accelerating the pace of change, and a growing population is putting more TABLE O F pressure on our planet’s resources. Through science and collaboration, Dow’s people are working across the Company to redefine the role of business by innovating solutions that advance business and society and by valuing nature in all of our decisions. With our 2025 Sustainability Goals, Dow is focused on CONTENTS Redefining the Role of Business in Society developing collaborative blueprints that integrate public policy solutions, science and technology, and value chain innovation. The aim is to build solutions between government, business and society that generate shared value and are long lasting, scalable and transformative. These 10-year goals are designed to harness Dow’s innovation strengths, global reach and the passion of our employees. WHO WE ARE WHY WE DO IT WHAT WE DO HOW WE DO IT INTRODUCTION ADDITIONAL GRI PROFILE GLOBAL OUR PRODUCTS OPERATIONS & INFORMATION CONTENT INDEX & PEOPLE CHALLENGES & OUR & SOLUTIONS PERFORMANCE COMMITMENTS The Dow Chemical Company dow.com/sustainability 4 2017 SUSTAINABILITY REPORT 5 ABOUT THE DOWDUPONT MERGER TRANSACTION AND INTENDED BUSINESS SEPARATIONS GRI 102-10 Dow (“the Company”) has been committed to transparently reporting our progress For the 15th year, Dow has applied the framework provided by the Global Reporting on sustainability for many years. This report reflects the Company’s performance for Initiative (GRI) to its annual sustainability report. GRI 102-52 This report has been Effective August 31, 2017, The Dow Chemical Company (“Dow”) and E.
    [Show full text]
  • Toxics in Carpets in the European Union
    Toxics in Carpets in the European Union Report Prepared by: Jessica Onyshko, Dr Rob Hewlett Report Approved by: Paul Ashford, Peter Scholes Date: March 2018 Disclaimer Anthesis Consulting Group PLC has prepared this report for the sole use of the client and for the intended purposes as stated in the agreement between Anthesis and the client under which this report was completed. Anthesis has exercised due and customary care in preparing this report but has not, save as specifically stated, independently verified information provided by others. No other warranty, express or implied, is made in relation to the contents of this report. The use of this report, or reliance on its content, by unauthorised third parties without written permission from Anthesis shall be at their own risk, and Anthesis accepts no duty of care to such third parties. Any recommendations, opinions or findings stated in this report are based on facts and circumstances as they existed at the time the report was prepared. Any changes in such facts and circumstances may adversely affect the recommendations, opinions or findings contained in this report. Anthesis Consulting Group, 2018 2 Toxics in Carpets in the European Union Prepared for: Prepared by: Stichting Changing Markets, Anthesis Consulting Group Oorsprongpark 12, 3581 ET, The Future Centre, Utrecht, 9 Newtec Place, Netherlands Magdalen Road, Oxford, OX4 1RE Report written by: Jessica Onyshko, Dr Rob Hewlett E-mail: [email protected] Website: www.anthesisgroup.com Tel: 01865 250818 Quality Assurance Fax: 01865 794586 Peter Scholes, Paul Ashford Company Registration 08425819 Report approved by: Paul Ashford, Director March 2018 Anthesis Consulting Group, 2018 3 Anthesis Consulting Group Anthesis is a specialist global sustainability services and solutions provider founded on the belief that sustainable business practices are at the heart of long-term commercial success.
    [Show full text]
  • View Annual Report
    2016 DUPONT ANNUAL REPORT A world leader in science and innovation, DuPont continues to work toward sustainable, renewable and market-driven solutions for some of our biggest global challenges. We are helping to provide healthy food for people everywhere, decreasing dependence on fossil fuels, and protecting life and the environment. For more than two centuries, our ability to meet the changing needs of our customers and society through world-class science and innovation has been the key to our success. DuPont’s current transformation will position each of our businesses with a clear focus and allow us to deliver superior solutions and choices for our customers. For additional information about DuPont and its commitment to inclusive innovation, please visit dupont.com. 2016 SEGMENT NET SALES 2016 TOTAL SEGMENT [U.S. DOLLARS IN MILLIONS] OPERATING EARNINGS AND TOTAL SEGMENT OPERATING MARGINS [1] $147 $6,000 19.5% $2,954 19.0% $5,000 18.5% $4,000 18.0% $5,249 $9,516 $3,000 17.5% MARGIN 17.0% $2,000 $ IN MILLIONS 16.5% $1,000 $3,268 16.0% $1,960 $0 15.5% 2015 2016 $1,500 AGRICULTURE NUTRITION & HEALTH ELECTRONICS & COMMUNICATIONS PERFORMANCE MATERIALS INDUSTRIAL BIOSCIENCES PROTECTION SOLUTIONS OTHER 2016 OPERATING MARGINS BY SEGMENT 30% 25% 20% 15% 10% 5% 0% RE TRIAL ALTH 2015 US AGRICULTU PROTECTIONSOLUTIONS 2016 ELECTRONICS & IND NUTRITION & HE PERFORMANCE COMMUNICATIONS BIOSCIENCES MATERIALS Dear DuPont Shareowners, 2016 was a year of transformation and accomplishment for DuPont. We made meaningful progress improving the fundamentals of the business and significantly strengthened our competitive position, so that we started 2017 operating at a new standard of excellence.
    [Show full text]
  • Innovating Through Alliance: a Case Study of the Dupont–BP Partnership on Biofuels
    White Paper Innovating through Alliance: A Case Study of the DuPont–BP Partnership on Biofuels By Truman Semans and Andre de Fontaine September 2009 Truman Semans is a Principal of GreenOrder, a business strategy consulting firm focused on environmental sustainability whose clients have included GE, major utilities, BP, DuPont, HP, and GM. He has advised dozens of Fortune 500 corporations on energy, climate, technology, and policy. He has served on the Executive Committee of US Climate Action Partnership and, while at the U.S. Treasury, as a member of the U.S. Climate Change Negotiating Team. Truman consulted with McKinsey & Co., and he serves on the Board of Directors of the National Climate Trust, the Board of Visitors of the Nicholas School of the Environment, and the Chesapeake Bay Foundation President's Advisory Committee. Andre de Fontaine is a Markets and Business Strategy Fellow at the Pew Center on Global Climate Change. 1 White Paper – Pew Center on Global Climate Change Preface—An Update Provided by DuPont and BP on their Advanced Biofuels Partnership This paper aims to provide insights into how industry alliances can deliver innovation and new technologies in an accelerated fashion. Since work began on this paper, the relationship between DuPont and BP has evolved significantly. This preface to the case study, written by representatives from DuPont and BP, provides an update on the companies’ work together. In June 2009, the partners formalized their relationship and created Butamax Advanced Biofuels LLC, a joint venture tasked with completing the technology development and commercialization of biobutanol – the advanced, premium biofuel molecule that had catalyzed the companies’ initial partnership.
    [Show full text]
  • Dupont Nutrition Food Ingredients (Beijing) Co., Ltd
    Certificate of Approval This is to certify that the Management System of: E. I. du Pont de Nemours and Company 974 Centre Road, Wilmington, DE, 19805, United States has been approved by LRQA to the following standards: ISO 14001:2015 Chris Koci Issued By: Lloyd's Register Quality Assurance, Inc. This certificate is valid only in association with the certificate schedule bearing the same number on which the locations applicable to this approval are listed. Current Issue Date: 21 January 2018 Original Approvals: Expiry Date: 20 January 2021 ISO 14001 – 21 January 2009 Certificate Identity Number: 10052905 Approval Number(s): ISO 14001 – 0011717 The scope of this approval is applicable to: Manufacture of Science-Based Products for Agriculture, Nutrition, Electronics, Communications, Safety and Protection, Home and Construction, Transportation and Apparel Markets. Lloyd's Register Group Limited, its affiliates and subsidiaries, including Lloyd's Register Quality Assurance Limited (LRQA), and their respective officers, employees or agents are, individually and collectively, referred to in this clause as 'Lloyd's Register'. Lloyd's Register assumes no responsibility and shall not be liable to any person for any loss, damage or expense caused by reliance on the information or advice in this document or howsoever provided, unless that person has signed a contract with the relevant Lloyd's Register entity for the provision of this information or advice and in that case any responsibility or liability is exclusively on the terms and conditions set out in that contract. Issued By: Lloyd's Register Quality Assurance, Inc., 1330 Enclave Parkway, Suite 200, Houston, Texas 77077, United States Page 1 of 15 Certificate Schedule Certificate Identity Number: 10052905 Location Activities Global Headquarters ISO 14001:2015 974 Centre Road, Wilmington, DE, 19805, Headquarters Activities in Support of the Global United States Manufacturing Sites and EMS Oversight.
    [Show full text]
  • | Mit Kudo Tori Ta on Olla Tal Vai Ai Ai
    |MIT KUDO TORI USTA ON20170326842A1OLLA TAL VAI AI AI AIR ( 19) United States (12 ) Patent Application Publication (10 ) Pub. No. : US 2017/ 0326842 A1 MORITZ et al. ( 43 ) Pub . Date: Nov . 16 , 2017 ( 54 ) POLYESTER FILM INCORPORATING B650 25 / 14 (2006 . 01) SILICONE FOR RELEASE OF CANNED B32B 7 / 12 (2006 . 01) MEAT PRODUCTS ( 52 ) U . S . CI. CPC .. B32B 15 / 09 ( 2013 .01 ) ; B65D 25/ 14 ( 71 ) Applicant : TORAY PLASTICS (AMERICA ) , (2013 . 01) ; B32B 15 / 18 (2013 .01 ) ; B32B 7 / 12 INC ., North Kingstown , RI (US ) ( 2013 .01 ) ; B650 81 /24 (2013 . 01 ) ; B65D 81 / 3415 ( 2013 .01 ) ; C08 ) 5 / 18 ( 2013 . 01 ) ; (72 ) Inventors : Jan MORITZ , Bristol, RI (US ) ; Pat B32B 2307 / 748 (2013 . 01 ) ; B32B 2439 / 70 ANDREWS , West Kingstown, RI (US ) ; (2013 . 01 ) ; B32B 2307 /31 ( 2013 .01 ) ; C08J Keunsuk P . CHANG , North Kingstown , RI (US ) ; Christopher 2367 /02 (2013 . 01 ) ; CO8J 2483/ 04 (2013 . 01 ) NOTHNAGLE , Saunderstown, RI (US ) ; Stefanos SAKELLARIDES , East (57 ) ABSTRACT Greenwich , RI (US ) Embodiments herein relates to a Bisphenol A - free multi layer biaxially oriented polyester (BOPET ) film , for lami @( 73 ) Assignee : TORAY PLASTICS ( AMERICA ) , nation on metal sheets , which could be used for food INC . containers . The BOPET film has at least one outer release layer , which aids in the release of food , such as a high ( 21) Appl. No .: 15/ 663, 142 protein food source , when food is cooked and sterilized in direct contact with the outer release layer . The BOPET film (22 ) Filed : Jul. 28 , 2017 can be laminated to metals used in the manufacture of food Related U .
    [Show full text]