25Th Symposium on Biotechnology for Fuels and Chemicals Office of the Biomass Program, U.S
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Sponsors 25th Symposium on Biotechnology for Fuels and Chemicals for 25th Symposium on Biotechnology Office of the Biomass Program, U.S. Department of Energy U. S. Department of Agriculture (USDA) ARS National Renewable Energy Laboratory (NREL) Oak Ridge National Laboratory (ORNL) Argonne National Laboratory (ANL) Idaho National Engineering and Environmental Laboratory (INEEL) Pacific Northwest National Laboratory (PNNL) Alltech Archer Daniels Midland BBI International Biotechnology Industry Organization (BIO) Breckenridge Brewery Cargill, Inc. Cargill-Dow LLC Coors Brewing Company Corn Refiners Association E.I. du Pont de Nemours & Company, Inc. Genencor Iogen Corporation Katzen International Natural Resources, Canada Novozymes Biotech Procter & Gamble Syngenta Tate & Lyle Tembec Industries Prepared by National Renewable Energy Laboratory Golden, Colorado 80041 www.nrel.gov/biotech_symposium/ NREL/BK-510-33708 Printed with a renewable-source ink on paper containing at least 50% wastepaper, including 20% postconsumer waste Organizing Committee Members: Mark Finkelstein, Conference Chairman Welcome National Renewable Energy Laboratory Golden, Colorado from the Organizing Committee Brian Davison, Conference Co-chairman Oak Ridge, National Laboratory Improving the economics of producing fuels and chemicals is vital to many industrial sectors. Oak Ridge, Tennessee We have designed the program for the 25th Symposium on Biotechnology for Fuels and William Apel Chemicals to deliver the latest research breakthroughs and results in biotechnology Idaho National Engineering that stimulate such improvements. Whether you represent the industrial, academic, or and Environmental Laboratory government sector, we invite you to join us and participate in this exciting exchange of Idaho Falls, Idaho information and ideas. You will find valuable opportunities for productive interactions with Doug Cameron your colleagues, both from a national and international perspective. Cargill, Inc. Minneapolis, Minnesota With the 25th Symposium, we continue the tradition of providing an informal, congenial Thomas Jeffries atmosphere that our participants find conducive to technically discussing program topics. USDA–Forest Service Madison, Wisconsin This year technical topics include: James Lee Oak Ridge National Laboratory Session 1A. Feedstock Supply, Logistics, Processing, and Composition Oak Ridge, Tennessee Session 1B. Enzyme Catalysis and Engineering Lee Lynd Session 2. Microbial Catalysis and Engineering Dartmouth College Hanover, New Hampshire Session 3. Bioprocessing including Separations James McMillan Session 4. Biotechnology for Fuels and Chemicals – Past, Present, and Future National Renewable Energy Laboratory Golden, Colorado Session 5. Biobased Industrial Chemicals Dale Monceaux Session 6A. Biomass Preteatment and Hydrolysis Katzen International, Inc. Session 6B. Plant Biotechnology and Feedstock Genomics Cincinnati, Ohio Special Topics A. Microbial Pentose Metabolism Mark Paster U.S. Department of Energy Special Topics B. International Bioenergy Agency Bioethanol Meeting Washington, DC Jack Saddler This year we will augment our technical program with an exciting presentation by Dr. J. Craig University of British Columbia Venter, Genome Sequencer, Entrepreneur, and Chief Executive Officer. The title of his Vancouver, British Columbia, Canada presentation is “Genomic Approaches to the Environment.” Valerie Sarisky-Reed U.S. Department of Energy Each year at this Symposium we recognize an individual who has distinguished himself Washington, DC or herself in the application of biotechnology to produce fuels and chemicals. This award Sharon Shoemaker acknowledges contributions to the field as a whole or this symposium, particularly innovation University of California Davis, California in fundamental and applied biotechnology, insight into bioprocessing fundamentals, or commitment to facilitate commercialization of products from renewable resources. This David Short DuPont Inc. award is named in honor of Dr. Charles D. Scott, founder of the Biotechnology Symposium Newark, Delaware for Fuels and Chemicals and its chair for the first ten years. In his years of work at ORNL, Jeff Tolan Chuck performed research and development on many novel bioprocessing systems Iogen Corporation including high production bioreactors, immobilized microbes, enzymes in organic media, Ottawa, Ontario, Canada and a coal bioprocess to name a few. The award is presented annually at the BSFC to Nancy Watlington recognized persons who have distinguished themselves in the area of biotechnology to Oak Ridge National Laboratory produce fuels and chemicals. See page ‘i’ for information on this years winner. Oak Ridge, Tennessee Liz Willson As always, we have included events to provide you with opportunities to socialize with your National Renewable Energy Laboratory colleagues. You will also have opportunities to explore Colorado’s Rocky Mountains. Ask at Golden, Colorado the registration desk for more information. Charles Wyman Dartmouth College Hanover, New Hampshire Notice This report was prepared as an account of work sponsored by an agency of the United States government. Neither the United States government nor any agency thereof, nor any of their employees, makes anywarranty, express or implied, Guido Zacchi or assumes any legal liability or responsibility for the accuracy, completeness, or usefulness of any information, apparatus, Lund University product, or process disclosed, or represents that its use would not infringe privately owned rights. Reference herein to any Lund, Sweden specific commercial product, process, or service by trade name, trademark, manufacturer, or otherwise does not necessarily constitute or imply its endorsement, recommendation, or favoring by the United States government or any agency thereof. Gisella M. Zanin The views and opinions of authors expressed herein do not necessarily state or reflect those of the United States government State University of Maringa or any agency thereof. Maringa, Parana, Brazil Charles D. Scott Award Charles D. Scott Award Through the course of his professional career, Tom Jeffries has worked on many aspects of bioenergy and biomass conversion. Tom’s fundamental interest in primary productivity is refl ected in his MS work with photosynthetic bacteria from 1969-1971 and in his work at on the biophotolytic production of hydrogen by blue-green algae. During doctoral studies on yeast cell wall lysis at Rutgers University from 1972-1975, Tom had the good fortune of working with James D. Macmillan, Elwin Reese, Mary Mandels and Doug Eveleigh, all of whom spurred a life-long interest in enzymatic hydrolysis of cellulose, hemicellulose and complex polysaccharides. While at the Lawrence Livermore National Laboratory from 1976-1977, Tom worked with a highly interdisciplinary team to assess the magnitude of the U.S. biomass resource. In post-doctoral studies with Harry Gregor in the Department of Chemical Engineering at Columbia University from 1978-1979, Tom developed membrane-coupled anaerobic fermentations of cellulose for the production of methane and short chain organic acids. In 1979, Tom joined Kent Kirk at the Forest Products Laboratory (FPL) where he worked for several years on the regulation of lignin biodegradation. In the early 1980’s the FPL ethanol program under the guidance of Jerry Saeman enabled Tom to return to studies on yeast physiology and regulation. His research into the oxygen requirements for yeast xylose fermentations has continued to this day. This has led to increased knowledge of xylose metabolism and improved recombinant yeasts, especially Pichia stipitis. Tom has also mentored many students both at FPL and the University of Wisconsin. In this Symposium, Tom has served in many roles since the earliest meetings. Tom’s life has been blessed by the support and encouragement of his wife, Giovanna Miceli-Jeffries and by the love of their three daughters, Angelica, Carla and Francesca. 25th Symposium on Biotechnology for Fuels and Chemicals Program and Abstracts i ii Program and Abstracts 25th Symposium on Biotechnology for Fuels and Chemicals 25th Symposium on Biotechnology for Fuels and Chemicals Program and Abstracts iii Schedule at a Glance ��������� �� ������������� ��� ����� ��� ��������� Conference Program ������ �� ��� �������� ��������� ������ ���������� Sunday, May 4, 2003 10:00 am - 5:00 pm Registration/Editor’s Desk Open Foyer, 3rd Floor 10:00 am - 8:00 pm Poster Session/Exhibits Set-up Breckenridge Ballroom 1:00 am - 1:10 pm Opening Remarks Peaks 1 - 5, 3rd Floor 1:10 pm - 5:00 pm Session 1A and Session 1B (Concurrent Sessions) Peaks 1 - 5, 3rd Floor 5:30 pm - 8:30 pm Welcoming Reception Lower Pool Area Monday, May 5, 2003 7:30 am - 5:00 pm Registration/Editor’s Desk Open Foyer, 3rd Floor 7:15 am - 8:00 am Continental Breakfast South Foyer, 3rd Floor 7:15 am - 8:00 am Speakers’ Breakfast Peak 6 - 7, 2nd Floor 8:00 am - 12:00 pm Session 2 Peaks 1 - 5, 3rd Floor 12:00 pm - 1:00 pm Lunch On Your Own 2:00 pm - 4:00 pm Special Topics Peaks 1 - 5, 3rd Floor Session A and Session B (Concurrent Sessions) 5:30 pm - 10:00 pm Poster Reception Breckenridge Ballroom Tuesday, May 6, 2003 7:30 am - 1:00 pm Registration/Editor’s Desk Open Foyer, 3rd Floor 7:15 am - 8:00 am Continental Breakfast South Foyer, 3rd Floor 7:15 am - 8:00 am Speakers’ Breakfast Peak 6 - 7, 2nd Floor 8:00 am - 12:00 pm Session 3 Peaks 1 - 5, 3rd Floor 12:00 pm - 1:30 pm Organizing Committee Luncheon and Meeting Peak 6 - 7,