DOCSLIB.ORG

A Novel Hydroxamic Acid-Containing Antibiotic Produced by a Saharan Soil-Living Streptomyces Strain A

Total Page：16

File Type：pdf, Size：1020Kb

Download full-text PDF Read full-text
A Novel Hydroxamic Acid-Containing Antibiotic Produced by a Saharan Soil-Living Streptomyces Strain A A novel hydroxamic acid-containing antibiotic produced by a Saharan soil-living Streptomyces strain A. Yekkour, A. Meklat, Christian Bijani, O. Toumatia, R. Errakhi, A. Lebrihi, Florence Mathieu, Abdelghani Zitouni, Nasserdine Sabaou To cite this version: A. Yekkour, A. Meklat, Christian Bijani, O. Toumatia, R. Errakhi, et al.. A novel hydroxamic acid-containing antibiotic produced by a Saharan soil-living Streptomyces strain. Letters in Applied Microbiology, Wiley, 2015, 60 (6), pp.589-596. 10.1111/lam.12412. hal-01938007 HAL Id: hal-01938007 https://hal.archives-ouvertes.fr/hal-01938007 Submitted on 20 May 2019 HAL is a multi-disciplinary open access L’archive ouverte pluridisciplinaire HAL, est archive for the deposit and dissemination of sci- destinée au dépôt et à la diffusion de documents entific research documents, whether they are pub- scientifiques de niveau recherche, publiés ou non, lished or not. The documents may come from émanant des établissements d’enseignement et de teaching and research institutions in France or recherche français ou étrangers, des laboratoires abroad, or from public or private research centers. publics ou privés. . . . . A novel hydroxamic acid-containing antibiotic produced by Streptomyces a Saharan soil-living strain 1,2 1 3 1 4 4 5 1 A. Yekkour , A. Meklat , C. Bijani , O. Toumatia , R. Errakhi , A. Lebrihi , F. Mathieu , A. Zitouni 1 and N. Sabaou 1 Laboratoire de Biologie des Systemes Microbiens (LBSM), Ecole Normale Superieure de Kouba, Alger, Algeria 2 Centre de Recherche Polyvalent, Institut National de la Recherche Agronomique d’Algerie, Alger, Algeria 3 Laboratoire de Chimie de Coordination (LCC), CNRS, Universite de Toulouse, UPS, INPT, Toulouse, France 4 Universite Moulay Ismail, Meknes, Morocco 5 Universite de Toulouse, Laboratoire de Genie Chimique UMR 5503 (CNRS/INPT/UPS), INP de Toulouse/ENSAT, Castanet-Tolosan Cedex, France Significance and Impact of the Study: This study presents the isolation of a Streptomyces strain, named WAB9, from a Saharan soil in Algeria. This strain was found to produce a new hydroxamic acid-contain- ing molecule with interesting antimicrobial activities towards various multidrug-resistant micro-organ- isms. Although hydroxamic acid-containing molecules are known to exhibit low toxicities in general, only real evaluations of the toxicity levels could decide on the applications for which this new molecule is potentially most appropriate. Thus, this article provides a new framework of research. Keywords Abstract antimicrobial activity, hydroxamic acid, Streptomyces, structure elucidation, During screening for potentially antimicrobial actinobacteria, a highly taxonomy. antagonistic strain, designated WAB9, was isolated from a Saharan soil of Algeria. A polyphasic approach characterized the strain taxonomically as a Correspondence member of the genus Streptomyces. The strain WAB9 exhibited a broad Nasserdine Sabaou, Laboratoire de Biologie spectrum of antimicrobial activity toward various multidrug-resistant micro- des Systemes Microbiens, Ecole Normale organisms. A PCR-based assay of genomic potential for producing bioactive Superieure de Kouba, Alger 16050, Algeria. E-mail: [email protected] metabolites revealed the presence of PKS-II gene. After 6 days of strain fermentation, one bioactive compound was extracted from the remaining aqueous phase and then purified by HPLC. The chemical structure of the compound was determined by spectroscopic (UV–visible, and 1H and 13C doi:10.1111/lam.12412 NMR) and spectrometric analysis. The compound was identified to be 2-amino-N-(2-amino-3-phenylpropanoyl)-N-hydroxy-3-phenylpropanamide, a novel hydroxamic acid-containing molecule. The pure molecule showed appreciable minimum inhibitory concentration values against a selection of drug-resistant bacteria, filamentous fungi and yeasts. Introduction respect to their effects against pathogenic strains and have led to the discovery of a large number of novel active Actinobacteria are a group of ubiquitous Gram-positive compounds of considerable value (Solecka et al. 2012). bacteria with a percentage of guanine–cytosine higher However, the present context of both a decline in the dis- than 55%, and most of them produce mycelia. Because of covery of new antibiotics and an increase in drug-resis- their ability to provide a broad range of bioactive com- tant pathogens makes the screening for new antibiotic- pounds, these micro-organisms have attracted much producing Streptomyces essential. interest. Members of this group, in particular those One of the strategies for enhancing the likelihood of belonging to the genus Streptomyces, are considered as the obtaining particularly interesting isolates and secondary most important producers of bioactive molecules. There- metabolites is to analyse extreme habitats (Santhanam fore, Streptomyces have been thoroughly investigated with et al. 2012), such as arid Saharan soils. Previous surveys on the ecological distribution of Actinomycetes in Alge- Table 1 Antagonistic properties of the strain WAB9 against various multidrug-resistant micro-organisms rian Saharan soils have already demonstrated their appre- ciable biodiversity (Sabaou et al. 1998) and permitted Target micro-organism new species and new antibiotics to be found (Boubetra (antibiotic resistance pattern*) Activity† (mm) et al. 2013; Meklat et al. 2013). Bacillus subtilis ATCC 6633 (NEO) 39 Hydroxamic acids (bearing the functional group N- Staphylococcus aureus S1 28 hydroxy amide) are one such interesting class of molecule (CAR, GEN, K, NEO, OLE, SPI, VAN) produced by many Streptomyces species (Dimkpa et al. Enterobacter cloacae E13 (AMC, ATM, CEP, 29 2008; Kodani et al. 2013). Their low toxicities in general, CTX, FEP, FOX, GEN, PIP, TCC, TIC, TOB) their weak acid properties and their ability for complex- Escherichia coli E52 (ATM, CAZ, CEP, CTX, 35 ing transition metal ions have resulted in these molecules FEP, GEN, PIP, TIC, TOB) Klebsiella pneumoniae K44 (AMX, AN, CF, 29 receiving considerable attention for broad therapeutic and CRO, CTX, CXM, GEN, K, MZ, SSS, TIC) industrial applications (Vanjari and Pande 2003; Rho Pseudomonas aeruginosa IPA1 (AMX, CAR, 29 et al. 2006; Jahangirian et al. 2011). Among their numer- GEN, NEO, SPI, SSS, VAN) ous properties, hydroxamic acids and derivatives have Salmonella enterica E32 (ATM, CAZ, CEP, 16 been largely reported as effective antibacterial and anti- CTX, FEP, GEM, PIP, TIC, TOB) fungal agents (Li et al. 2003; Pepeljnjak et al. 2005). These Fusarium culmorum (CHX, ITR, NYS, TIZ) 39 activities are connected with the ability of hydroxamic Fusarium moniliforme (AMB, CHX, NYS, TIZ) 29 Fusarium sporotrichoides 20 acids to potently and selectively inhibit a range of (AMB, CHX, ITR, NYS, TIZ, TER) enzymes that are vital for the survival of bacteria, such as Fusarium graminearum (CHX, NYS, TIZ) 25 various metalloproteinases, urease, cyclooxygenases and Fusarium oxysporum f. sp. lini (ITR,TIZ) 25 peroxidase (Muri et al. 2004). F. oxysporum f. sp. albedinis 26 Recently, exploration of microbial antibiotic potential (AMB, CHX, ITR, NYS, TIZ, TER) has focused on the screening of polyketide synthase (PKS) Fusarium proliferatum (CHX, ITR, NYS, TIZ) 22 and nonribosomal peptide synthetase (NRPS) genes. This Fusarium equiseti (CHX, TIZ) 30 Aspergillus carbonarius (CHX, NYS) 33 exploration approach seems applicable for hydroxamic Aspergillus niger (AMB, CHX, NYS, TIZ) 25 acid-containing molecules, since these molecules are Aspergillus flavus (TIZ) 26 already known to be synthesized through NRPS-depen- Aspergillus parasiticus (AMB, CHX, ITR, NYS, TIZ) 20 dent or independent pathways (Barry and Challis 2009; Penicillium glabrum (CHX) 38 Oves-Costales et al. 2009). Penicillium expansum 37 Here, we describe the isolation of a highly antagonistic Umbelopsis ramanniana (CHX, ITR, TIZ, TER) 36 Streptomyces strain, designated WAB9, from a Saharan soil Candida albicans IPA 200 (NYS, ITR, TIZ, CHX, TER) 15 Saccharomyces cerevisiae ATCC 4226 34 sample and its identification by conventional and molecu- lar methods, together with production and structure eluci- AMC, amoxicillin + clavulanic acid; AMX, amoxicillin; AN, amikacin; dation of the corresponding bioactive compound, which ATM, aztreonam; C, chloramphenicol; CAR, carbenicillin; CAZ, ceftazi- was found to be a new hydroxamic acid-containing antibi- dime; CFP, cefpirome; CRO, ceftriaxone; CTX, cefotaxime; CXM, ce- otic. The minimum inhibitory concentrations (MICs) of furoxime; FEP, cefepime; FOX, cefoxitin; GEN, gentamicin; K, kanamycin; MZ, mezlocillin; NEO, neomycin; OLE, oleandomycin PIP, this antibiotic were also evaluated on a set of various mul- piperacillin; SPI; spiramycin; SSS, sulfamide; TCC, ticarcillin + clavulan- tidrug-resistant bacteria, filamentous fungi and yeasts. ic acid; TIC, ticarcillin; TOB, tobramycin; VAN, vancomycin; AMB, amphotericin B; CHX, cycloheximide; ITR, itraconazole; NYS, nystatin; TIZ, thioconazole; TER, terbinafine. Results and discussion *The antibiotic resistance patterns were evaluated in house as The strain WAB9 was isolated by plating a 2 : 10 serial described by Touati et al. (2006). dilution of the soil sample of Bechar on chitin-vitamin †Activity estimated by measuring the length of inhibition between agar medium supplemented with selective antibiotics. Actinomycetes and target micro-organisms. This strain was found in only a single small colony, which shows it rarity. the targeted bacteria, filamentous fungi and
Load more

Recommended publications
  • Identification and Antibiosis of a Novel Actinomycete Strain RAF-11 Isolated from Iraqi Soil
    View metadata, citation and similar papers at core.ac.uk brought to you by CORE provided by GSSRR.ORG: International Journals: Publishing Research Papers in all Fields International Journal of Sciences: Basic and Applied Research (IJSBAR) ISSN 2307-4531 http://gssrr.org/index.php?journal=JournalOfBasicAndApplied Identification and Antibiosis of a Novel Actinomycete Strain RAF-11 Isolated From Iraqi Soil. R. FORAR. LAIDIa, A. ABDERRAHMANEb, A. A. HOCINE NORYAc. a Department of Natural Sciences, Ecole Normale Superieure, Vieux-Kouba, Algiers – Algeria b,c Institute of Genetic Engineering and Biotechnology, Baghdad - Iraq. a email: [email protected] Abstract A total of 35 actinomycetes strains were isolated from and around Baghdad, Iraq, at a depth of 5-10 m, by serial dilution agar plating method. Nineteen out of them showed noticeable antimicrobial activities against at least, to one of the target pathogens. Five among the nineteen were active against both Gram positive and Gram negative bacteria, yeasts and moulds. The most active isolate, strain RAF-11, based on its largest zone of inhibition and strong antifungal activity, especially against Candida albicans and Aspergillus niger, the causative of candidiasis and aspergillosis respectively, was selected for identification. Morphological and chemical studies indicated that this isolate belongs to the genus Streptomyces. Analysis of the 16S rDNA sequence showed a high similarity, 98 %, with the most closely related species, Streptomyces labedae NBRC 15864T/AB184704, S. erythrogriseus LMG 19406T/AJ781328, S. griseoincarnatus LMG 19316T/AJ781321 and S. variabilis NBRC 12825T/AB184884, having the closest match. From the taxonomic features, strain RAF-11 matched with S. labedae, in the morphological, physiological and biochemical characters, however it showed significant differences in morphological characteristics with this nearest species, S.
    [Show full text]
  • In Vitro Antagonistic Activity of Soil Streptomyces Collinus Dpr20 Against Bacterial Pathogens
    IN VITRO ANTAGONISTIC ACTIVITY OF SOIL STREPTOMYCES COLLINUS DPR20 AGAINST BACTERIAL PATHOGENS Pachaiyappan Saravana Kumar1, Michael Gabriel Paulraj1, Savarimuthu Ignacimuthu1,3*, Naif Abdullah Al-Dhabi2, Devanathan Sukumaran4 Address(es): Dr. Savarimuthu Ignacimuthu, 1Division of Microbiology, Entomology Research Institute, Loyola College, Chennai, India-600 034. 2Department of Botany and Microbiology, Addiriyah Chair for Environmental Studies, College of Science, King Saud University, P.O. Box 2455, Riyadh 11451, Saudi Arabia. 3International Scientific Program Partnership (ISPP), King Saud University, Riyadh11451, Saudi Arabia 4Vector Management Division, Defence Research and Development Establishment, Gwalior, Madhya Pradesh, India. *Corresponding author: [email protected] doi: 10.15414/jmbfs.2017/18.7.3.317-324 ARTICLE INFO ABSTRACT Received 20. 9. 2017 Actinomycetes are one of the most important groups that produce useful secondary metabolites. They play a great role in pharmaceutical Revised 2. 11. 2017 and industrial uses. The search for antibiotic producing soil actinomycetes to inhibit the growth of pathogenic microorganisms has Accepted 6. 11. 2017 become widespread due to the need for newer antibiotics. The present work was aimed to isolate soil actinomycetes from pinus tree Published 1. 12. 2017 rhizosphere from Doddabetta, Western Ghats, Tamil Nadu, India. Thirty one actinomycetes were isolated based on heterogeneity and stability in subculturing; they were screened against 5 Gram positive and 7 Gram negative bacteria in an in vitro antagonism assay. In the preliminary screening, out of 31 isolates, 12.09% showed good antagonistic activity; 25.08% showed moderate activity; 19.35% Regular article showed weak activity and 41.93% showed no activity against the tested bacteria. Among the isolates tested, DPR20 showed good antibacterial activity against both Gram positive and Gram negative bacteria.
    [Show full text]
  • DAFTAR PUSTAKA Abidin, Z. A. Z., A. J. K. Chowdhury
    160 DAFTAR PUSTAKA AKTINOMISETES PENGHASIL ANTIBIOTIK DARI HUTAN BAKAU TOROSIAJE GORONTALO YULIANA RETNOWATI, PROF. DR. A. ENDANG SUTARININGSIH SOETARTO, M.SC; PROF. DR. SUKARTI MOELJOPAWIRO, M.APP.SC; PROF. DR. TJUT SUGANDAWATY DJOHAN, M.SC Universitas Gadjah Mada, 2019 | Diunduh dari http://etd.repository.ugm.ac.id/ Abidin, Z. A. Z., A. J. K. Chowdhury, N. A. Malek, and Z. Zainuddin. 2018. Diversity, antimicrobial capabilities, and biosynthetic potential of mangrove actinomycetes from coastal waters in Pahang, Malaysia. J. Coast. Res., 82:174–179 Adegboye, M. F., and O. O. Babalola. 2012. Taxonomy and ecology of antibiotic producing actinomycetes. Afr. J. Agric. Res., 7(15):2255-2261 Adegboye, M.,F., and O. O. Babalola. 2013. Actinomycetes: a yet inexhausative source of bioactive secondary metabolites. Microbial pathogen and strategies for combating them: science, technology and eductaion, (A.Mendez-Vila, Ed.). Pp. 786 – 795. Adegboye, M. F., and O. O. Babalola. 2015. Evaluation of biosynthesis antibiotic potential of actinomycete isolates to produces antimicrobial agents. Br. Microbiol. Res. J., 7(5):243-254. Accoceberry, I., and T. Noel. 2006. Antifungal cellular target and mechanisms of resistance. Therapie., 61(3): 195-199. Abstract. Alongi, D. M. 2009. The energetics of mangrove forests. Springer, New Delhi. India Alongi, D. M. 2012. Carbon sequestration in mangrove forests. Carbon Management, 3(3):313-322 Amrita, K., J. Nitin, and C. S. Devi. 2012. Novel bioactive compounds from mangrove dirived Actinomycetes. Int. Res. J. Pharm., 3(2):25-29 Ara, I., M. A Bakir, W. N. Hozzein, and T. Kudo. 2013. Population, morphological and chemotaxonomical characterization of diverse rare actinomycetes in the mangrove and medicinal plant rhizozphere.
    [Show full text]
  • A Novel Hydroxamic Acid-Containing Antibiotic Produced by a Saharan Soil-Living Streptomyces Strain A
    View metadata, citation and similar papers at core.ac.uk brought to you by CORE provided by Open Archive Toulouse Archive Ouverte . . . . A novel hydroxamic acid-containing antibiotic produced by Streptomyces a Saharan soil-living strain 1,2 1 3 1 4 4 5 1 A. Yekkour , A. Meklat , C. Bijani , O. Toumatia , R. Errakhi , A. Lebrihi , F. Mathieu , A. Zitouni 1 and N. Sabaou 1 Laboratoire de Biologie des Systemes Microbiens (LBSM), Ecole Normale Superieure de Kouba, Alger, Algeria 2 Centre de Recherche Polyvalent, Institut National de la Recherche Agronomique d’Algerie, Alger, Algeria 3 Laboratoire de Chimie de Coordination (LCC), CNRS, Universite de Toulouse, UPS, INPT, Toulouse, France 4 Universite Moulay Ismail, Meknes, Morocco 5 Universite de Toulouse, Laboratoire de Genie Chimique UMR 5503 (CNRS/INPT/UPS), INP de Toulouse/ENSAT, Castanet-Tolosan Cedex, France Significance and Impact of the Study: This study presents the isolation of a Streptomyces strain, named WAB9, from a Saharan soil in Algeria. This strain was found to produce a new hydroxamic acid-contain- ing molecule with interesting antimicrobial activities towards various multidrug-resistant micro-organ- isms. Although hydroxamic acid-containing molecules are known to exhibit low toxicities in general, only real evaluations of the toxicity levels could decide on the applications for which this new molecule is potentially most appropriate. Thus, this article provides a new framework of research. Keywords Abstract antimicrobial activity, hydroxamic acid, Streptomyces, structure elucidation, During screening for potentially antimicrobial actinobacteria, a highly taxonomy. antagonistic strain, designated WAB9, was isolated from a Saharan soil of Algeria. A polyphasic approach characterized the strain taxonomically as a Correspondence member of the genus Streptomyces.
    [Show full text]
  • Study of Actinobacteria and Their Secondary Metabolites from Various Habitats in Indonesia and Deep-Sea of the North Atlantic Ocean
    Study of Actinobacteria and their Secondary Metabolites from Various Habitats in Indonesia and Deep-Sea of the North Atlantic Ocean Von der Fakultät für Lebenswissenschaften der Technischen Universität Carolo-Wilhelmina zu Braunschweig zur Erlangung des Grades eines Doktors der Naturwissenschaften (Dr. rer. nat.) genehmigte D i s s e r t a t i o n von Chandra Risdian aus Jakarta / Indonesien 1. Referent: Professor Dr. Michael Steinert 2. Referent: Privatdozent Dr. Joachim M. Wink eingereicht am: 18.12.2019 mündliche Prüfung (Disputation) am: 04.03.2020 Druckjahr 2020 ii Vorveröffentlichungen der Dissertation Teilergebnisse aus dieser Arbeit wurden mit Genehmigung der Fakultät für Lebenswissenschaften, vertreten durch den Mentor der Arbeit, in folgenden Beiträgen vorab veröffentlicht: Publikationen Risdian C, Primahana G, Mozef T, Dewi RT, Ratnakomala S, Lisdiyanti P, and Wink J. Screening of antimicrobial producing Actinobacteria from Enggano Island, Indonesia. AIP Conf Proc 2024(1):020039 (2018). Risdian C, Mozef T, and Wink J. Biosynthesis of polyketides in Streptomyces. Microorganisms 7(5):124 (2019) Posterbeiträge Risdian C, Mozef T, Dewi RT, Primahana G, Lisdiyanti P, Ratnakomala S, Sudarman E, Steinert M, and Wink J. Isolation, characterization, and screening of antibiotic producing Streptomyces spp. collected from soil of Enggano Island, Indonesia. The 7th HIPS Symposium, Saarbrücken, Germany (2017). Risdian C, Ratnakomala S, Lisdiyanti P, Mozef T, and Wink J. Multilocus sequence analysis of Streptomyces sp. SHP 1-2 and related species for phylogenetic and taxonomic studies. The HIPS Symposium, Saarbrücken, Germany (2019). iii Acknowledgements Acknowledgements First and foremost I would like to express my deep gratitude to my mentor PD Dr.
    [Show full text]
  • Actinobacteria and Myxobacteria Isolated from Freshwater Snails and Other Uncommon Iranian Habitats, Their Taxonomy and Secondary Metabolism
    Actinobacteria and Myxobacteria isolated from freshwater snails and other uncommon Iranian habitats, their taxonomy and secondary metabolism Von der Fakultät für Lebenswissenschaften der Technischen Universität Carolo-Wilhelmina zu Braunschweig zur Erlangung des Grades einer Doktorin der Naturwissenschaften (Dr. rer. nat.) genehmigte D i s s e r t a t i o n von Nasim Safaei aus Teheran / Iran 1. Referent: Professor Dr. Michael Steinert 2. Referent: Privatdozent Dr. Joachim M. Wink eingereicht am: 24.02.2021 mündliche Prüfung (Disputation) am: 20.04.2021 Druckjahr 2021 Vorveröffentlichungen der Dissertation Teilergebnisse aus dieser Arbeit wurden mit Genehmigung der Fakultät für Lebenswissenschaften, vertreten durch den Mentor der Arbeit, in folgenden Beiträgen vorab veröffentlicht: Publikationen Safaei, N. Mast, Y. Steinert, M. Huber, K. Bunk, B. Wink, J. (2020). Angucycline-like aromatic polyketide from a novel Streptomyces species reveals freshwater snail Physa acuta as underexplored reservoir for antibiotic-producing actinomycetes. J Antibiotics. DOI: 10.3390/ antibiotics10010022 Safaei, N. Nouioui, I. Mast, Y. Zaburannyi, N. Rohde, M. Schumann, P. Müller, R. Wink.J (2021) Kibdelosporangium persicum sp. nov., a new member of the Actinomycetes from a hot desert in Iran. Int J Syst Evol Microbiol (IJSEM). DOI: 10.1099/ijsem.0.004625 Tagungsbeiträge Actinobacteria and myxobacteria isolated from freshwater snails (Talk in 11th Annual Retreat, HZI, 2020) Posterbeiträge Myxobacteria and Actinomycetes isolated from freshwater snails and
    [Show full text]
  • Synthesis of Antibacterial Silver Nanoparticles From
    SYNTHESIS OF ANTIBACTERIAL SILVER NANOPARTICLES FROM ACTINOMYCETES ISOLATED FROM THIKA INDUSTRIAL WASTE DUMP SITES SOIL AND MOLECULAR IDENTIFICATION OF POTENTIAL ACTINOMYCETES ABEBE BIZUYE KASSAHUN DOCTOR OF PHILOSOPHY IN MOLECULAR BIOLOGY AND BIOTECHNOLOGY PAN AFRICAN UNIVERSITY INSTITUTE FOR BASIC SCIENCES, TECHNOLOGY AND INNOVATION 2018 SYNTHESIS OF ANTIBACTERIAL SILVER NANOPARTICLES FROM ACTINOMYCETES ISOLATED FROM THIKA INDUSTRIAL WASTE DUMP SITES SOIL AND MOLECULAR IDENTIFICATION OF POTENTIAL ACTINOMYCETES Abebe Bizuye Kassahun MB400-0005/15 A Thesis submitted to Pan African University Institute for Basic Sciences, Technology and Innovation in partial fulfillment of the requirements for the degree of Doctor of Philosophy in Molecular Biology and Biotechnology 2018 DECLARATION Statement by the student I, declare that this thesis submitted to the Pan African University Institute of Basic Sciences, Technology and Innovation in partial fulfillment of the requirements for the degree of doctor of philosophy in Molecular Biology and Biotechnology, is original research work done by me under the supervision and guidance of my supervisors, except where due acknowledgement is made in the text; to the best of my knowledge it has not been submitted to in this institution or other institutions seeking for similar degree or other purposes. Student name: Abebe Bizuye Kassahun Signature---------------Date--------------------- ID: MB400-0005/2015 This thesis has been submitted with our approval as university supervisors. 1. Professor Naomi Maina Signature------------------------Date------------------ JKUAT, Nairobi, Kenya 2. Professor Erastus Gatebe Signature-----------------------Date---------------- KIRDI, Nairobi, Kenya 3. Doctor Christine Bii Signature-----------------------Date------------------ KEMRI, Nairobi, Kenya iii DEDICATION I dedicated this work to my parents and family for their support and encouragement throughout my studies.
    [Show full text]
  • Investigating the Relationship Between Amphotericin B and Extracellular
    Investigating the relationship between amphotericin B and extracellular vesicles produced by Streptomyces nodosus By Samuel John King A thesis submitted in partial fulfilment of the requirements for the degree of Master of Research School of Science and Health Western Sydney University 2017 Acknowledgements A big thank you to the following people who have helped me throughout this project: Jo, for all of your support over the last two years; Ric, Tim, Shamilla and Sue for assistance with electron microscope operation; Renee for guidance with phylogenetics; Greg, Herbert and Adam for technical support; and Mum, you're the real MVP. I acknowledge the services of AGRF for sequencing of 16S rDNA products of Streptomyces "purple". Statement of Authentication The work presented in this thesis is, to the best of my knowledge and belief, original except as acknowledged in the text. I hereby declare that I have not submitted this material, either in full or in part, for a degree at this or any other institution. ……………………………………………………..… (Signature) Contents List of Tables............................................................................................................... iv List of Figures .............................................................................................................. v Abbreviations .............................................................................................................. vi Abstract .....................................................................................................................
    [Show full text]
  • Production of Vineomycin A1 and Chaetoglobosin a by Streptomyces Sp
    Production of vineomycin A1 and chaetoglobosin A by Streptomyces sp. PAL114 Adel Aouiche, Atika Meklat, Christian Bijani, Abdelghani Zitouni, Nasserdine Sabaou, Florence Mathieu To cite this version: Adel Aouiche, Atika Meklat, Christian Bijani, Abdelghani Zitouni, Nasserdine Sabaou, et al.. Produc- tion of vineomycin A1 and chaetoglobosin A by Streptomyces sp. PAL114. Annals of Microbiology, Springer, 2015, 65 (3), pp.1351-1359. 10.1007/s13213-014-0973-1. hal-01923617 HAL Id: hal-01923617 https://hal.archives-ouvertes.fr/hal-01923617 Submitted on 15 Nov 2018 HAL is a multi-disciplinary open access L’archive ouverte pluridisciplinaire HAL, est archive for the deposit and dissemination of sci- destinée au dépôt et à la diffusion de documents entific research documents, whether they are pub- scientifiques de niveau recherche, publiés ou non, lished or not. The documents may come from émanant des établissements d’enseignement et de teaching and research institutions in France or recherche français ou étrangers, des laboratoires abroad, or from public or private research centers. publics ou privés. Open Archive Toulouse Archive Ouverte OATAO is an open access repository that collects the work of Toulouse researchers and makes it freely available over the web where possible This is an author’s version published in: http://oatao.univ-toulouse.fr/20338 Official URL: https://doi.org/10.1007/s13213-014-0973-1 To cite this version: Aouiche, Adel and Meklat, Atika and Bijani, Christian and Zitouni, Abdelghani and Sabaou, Nasserdine and Mathieu, Florence Production of vineomycin A1 and chaetoglobosin A by Streptomyces sp. PAL114. (2015) Annals of Microbiology, 65 (3). 1351-1359.
    [Show full text]
  • INVESTIGATING the ACTINOMYCETE DIVERSITY INSIDE the HINDGUT of an INDIGENOUS TERMITE, Microhodotermes Viator
    INVESTIGATING THE ACTINOMYCETE DIVERSITY INSIDE THE HINDGUT OF AN INDIGENOUS TERMITE, Microhodotermes viator by Jeffrey Rohland Thesis presented for the degree of Doctor of Philosophy in the Department of Molecular and Cell Biology, Faculty of Science, University of Cape Town, South Africa. April 2010 ACKNOWLEDGEMENTS Firstly and most importantly, I would like to thank my supervisor, Dr Paul Meyers. I have been in his lab since my Honours year, and he has always been a constant source of guidance, help and encouragement during all my years at UCT. His serious discussion of project related matters and also his lighter side and sense of humour have made the work that I have done a growing and learning experience, but also one that has been really enjoyable. I look up to him as a role model and mentor and acknowledge his contribution to making me the best possible researcher that I can be. Thank-you to all the members of Lab 202, past and present (especially to Gareth Everest – who was with me from the start), for all their help and advice and for making the lab a home away from home and generally a great place to work. I would also like to thank Di James and Bruna Galvão for all their help with the vast quantities of sequencing done during this project, and Dr Bronwyn Kirby for her help with the statistical analyses. Also, I must acknowledge Miranda Waldron and Mohammed Jaffer of the Electron Microsope Unit at the University of Cape Town for their help with scanning electron microscopy and transmission electron microscopy related matters, respectively.
    [Show full text]
  • Isolation and Characterization of Actinobacteria with Potential for Control of Fusarium Spp and Colletotrichum Kahawae
    ISOLATION AND CHARACTERIZATION OF ACTINOBACTERIA WITH POTENTIAL FOR CONTROL OF FUSARIUM SPP AND COLLETOTRICHUM KAHAWAE JAMES OLUOCH NONOH A thesis submitted in partial fulfillment of the requirements for the degree of Master of Science in Microbiology of Jomo Kenyatta University of Agriculture and Technology 2007 Declaration This thesis is my original work and has not been presented for a degree in any other university. Signature………………………… Date……………………….. James Oluoch Nonoh This thesis has been submitted for examination with our approval as supervisors Signature………………………… Date……………………….. Prof. Hamadi Boga JKUAT, Kenya Signature………………………… Date………………………. Dr. Bernard A. Nyende JKUAT, Kenya Signature………………………… Date……………………….. Dr. Wilber Lwande ICIPE, Kenya Signature………………………… Date……………………….. Dr. Dan Masiga ICIPE, Kenya ii Dedication This work is dedicated to my late parents, Mr. and Mrs. Nonoh. Thank you for all the support you gave me. You laid in me a good foundation that has seen me through to this level of education. iii Acknowledgements I am greatly indebted to Du Pont Agricultural Enterprise (USA) for the research grant without which it would not have been possible to do this work. I would like to thank the International Center of Insect Physiology and Ecology (ICIPE), through the Director of Training and Capacity Development, Dr. J.P.R Ochieng Odero for awarding me the Dissertation Research Internship Program (DRIP) fellowship and for availing to me the use of the institutions facilities. I am pleased to express my acknowledgement to Dr. Wilber Lwande, Head of Bioprospecting Programme (ICIPE), for all the necessary support he gave me throughout my study period. I sincerely thank Kenya Wildlife Service (KWS) for giving me permission to collect samples from protected National Parks.
    [Show full text]
  • The Pangenome Diversity, Dynamics and Evolution of Genomes the Pangenome Hervé Tettelin • Duccio Medini Editors
    Hervé Tettelin Duccio Medini Editors The Pangenome Diversity, Dynamics and Evolution of Genomes The Pangenome Hervé Tettelin • Duccio Medini Editors The Pangenome Diversity, Dynamics and Evolution of Genomes Editors Hervé Tettelin Duccio Medini Department of Microbiology and GSK Vaccines R&D Immunology, Institute for Genome Siena, Italy Sciences University of Maryland School of Medicine Baltimore, Maryland, USA ISBN 978-3-030-38280-3 ISBN 978-3-030-38281-0 (eBook) https://doi.org/10.1007/978-3-030-38281-0 This book is an open access publication. © The Editor(s) (if applicable) and The Author(s) 2020. Open Access This book is licensed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence and indicate if changes were made. The images or other third party material in this book are included in the book’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the book’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. The use of general descriptive names, registered names, trademarks, service marks, etc. in this publication does not imply, even in the absence of a specific statement, that such names are exempt from the relevant protective laws and regulations and therefore free for general use.
    [Show full text]