RNF5 Antibody
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RNF5 Antibody CATALOG NUMBER: 27-144 Antibody used in WB on Human HeLa at 0.2-1 ug/ml. Specifications APPLICATIONS: RNF5 antibody can be used for detection of RNF5 by ELISA at 1:12500. RNF5 antibody can be used for detection of RNF5 by western blot at 1 ug/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000. USER NOTE: Optimal dilutions for each application to be determined by the researcher. POSITIVE CONTROL: 1) Cat. No. 1201 - HeLa Cell Lysate PREDICTED MOLECULAR 20 kDa WEIGHT: IMMUNOGEN: Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human RNF5. HOST SPECIES: Rabbit Properties PURIFICATION: Antibody is purified by peptide affinity chromatography method. PHYSICAL STATE: Lyophilized BUFFER: Antibody is lyophilized in PBS buffer with 2% sucrose. Add 50 uL of distilled water. Final antibody concentration is 1 mg/mL. CONCENTRATION: 1 mg/ml STORAGE CONDITIONS: For short periods of storage (days) store at 4˚C. For longer periods of storage, store RNF5 antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles. CLONALITY: Polyclonal CONJUGATE: Unconjugated Additional Info ALTERNATE NAMES: RNF5, RING5, RMA1 ACCESSION NO.: NP_008844 PROTEIN GI NO.: 5902054 OFFICIAL SYMBOL: RNF5 GENE ID: 6048 Background BACKGROUND: RNF5 contains a RING finger, which is a motif known to be involved in protein-protein interactions. This protein is a membrane-bound ubiquitin ligase. It can regulate cell motility by targeting paxillin ubiquitination and altering the distribution and localization of paxillin in cytoplasm and cell focal adhesions.The protein encoded by this gene contains a RING finger, which is a motif known to be involved in protein-protein interactions. This protein is a membrane-bound ubiquitin ligase. It can regulate cell motility by targeting paxillin ubiquitination and altering the distribution and localization of paxillin in cytoplasm and cell focal adhesions. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications. REFERENCES: 1) Bromberg, K.D., (2007) Cancer Res. 67 (17), 8172-8179. FOR RESEARCH USE ONLY December 12, 2016.