Recombinant Human HAT1 Protein Catalog Number: ATGP0507

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Recombinant human HAT1 protein Catalog Number: ATGP0507 PRODUCT INPORMATION Expression system E.coli Domain 20-341aa UniProt No. O14929 NCBI Accession No. NP_003633 Alternative Names Histone acetyltransferase 1, KAT1, Histone acetyltransferase 1 HAT 1, Histidine aminotransferase 1, Histone acetyltransferase type B catalytic subunit PRODUCT SPECIFICATION Molecular Weight 40.1 kDa (343aa) confirmed by MALDI-TOF Concentration 0.5mg/ml (determined by Bradford assay) Formulation Liquid in. 20mM Tris-HCl buffer (pH 8.0) containing 10% glycerol, 1mM DTT Purity > 90% by SDS-PAGE Tag His-Tag Application SDS-PAGE Storage Condition Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles. BACKGROUND Description HAT1, also known as histone acetyltransferase 1, is a type B histone acetyltransferase (HAT) that is involved in the rapid acetylation of newly synthesized cytoplasmic histones, which are in turn imported into the nucleus for de novo deposition onto nascent DNA chains. Histone acetylation, particularly of histone H4, plays an important role in replication-dependent chromatin assembly. Specifically, this HAT can acetylate soluble but not nucleosomal histone H4 at lysines 5 and 12, and to a lesser degree, histone H2A at lysine 5. Recombinant HAT1 1 Recombinant human HAT1 protein Catalog Number: ATGP0507 protein was expressed in E. coli and purified by using conventional chromatography techniques. Amino acid Sequence MGSSHHHHHH SSGLVPRGSH MKKLAEYKCN TNTAIELKLV RFPEDLENDI RTFFPEYTHQ LFGDDETAFG YKGLKILLYY IAGSLSTMFR VEYASKVDEN FDCVEADDVE GKIRQIIPPG FCTNTNDFLS LLEKEVDFKP FGTLLHTYSV LSPTGGENFT FQIYKADMTC RGFREYHERL QTFLMWFIET ASFIDVDDER WHYFLVFEKY NKDGATLFAT VGYMTVYNYY VYPDKTRPRV SQMLILTPFQ GQGHGAQLLE TVHRYYTEFP TVLDITAEDP SKSYVKLRDF VLVKLCQDLP CFSREKLMQG FNEDMAIEAQ QKFKINKQHA RRVYEILRLL VTD General References Verreault A., et al. (1998) Curr Biol. 8(2):96-108. Marmorstein R., et al. (2001) J Mol Biol. 311(3):433-44. DATA SDS-PAGE 3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain. 2.

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Expression of HAT1 and HDAC1, 2, 3 in Diffuse Large B-Cell Lymphomas, Peripheral T-Cell Lymphomas, and NK/T-Cell Lymphomas
The Korean Journal of Pathology 2012; 46: 142-150 ▒ ORIGINAL ARTICLE ▒ http://dx.doi.org/10.4132/KoreanJPathol.2012.46.2.142 Expression of HAT1 and HDAC1, 2, 3 in Diffuse Large B-Cell Lymphomas, Peripheral T-Cell Lymphomas, and NK/T-Cell Lymphomas Soo Kee Min · Young Ho Koh1 Background: It has generally been proven that histone acetylation and deacetylation are involved Yunwoong Park1 · Hyo Jung Kim2 in the malignant transformation. To date, however, this has rarely been studied in cases of malig- Jinwon Seo · Hye-Rim Park nant lymphoma. Methods: We studied nine cases of reactive lymphoid hyperplasia, 78 cases of diffuse large B-cell lymphoma (DLBCL), 13 cases of peripheral T-cell lymphoma, not otherwise 3 4 Seong Jin Cho · In Sun Kim specified (PTCL-NOS), and 13 cases of extranodal NK/T-cell lymphoma, nasal type (NKTCL). Thus, we attempted to elucidate the associations of the degree of the expression of histone acetyltrans- Department of Pathology, Hallym University ferase 1 (HAT1), histone deacetylase (HDAC) 1, HDAC2, and HDAC3 with the clinical behaviors of Sacred Heart Hospital, Hallym University College above malignant lymphomas using the immunohistochemistry and a western blot analysis. of Medicine; 1Ilsong Institute of Life Science, Re- Hallym University; 2Department of Hemato- sults: The degree of the expression of HAT1 was higher in cases of DLBCL, PTCL-NOS or NKT- Oncology, Hallym University Sacred Heart CL as compared with reactive lymphoid hyperplasia (p< 0.05). The degree of the expression of Hospital, Hallym University College of Medicine, HAT1 was correlated with that of HDAC1 in cases of DLBCL or NKTCL (p< 0.05).
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HAT1 Sirna (Rat)
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