DOCSLIB.ORG

“Development of Natural Actives for Spa and Cosmetic Products”

Total Page：16

File Type：pdf, Size：1020Kb

Download full-text PDF Read full-text
“Development of Natural Actives for Spa and Cosmetic Products” ““DevelopmentDevelopment ofof NaturalNatural ActivesActives forfor SpaSpa andand CosmeticCosmetic ProductsProducts ”” Chia Wen Chen Director of Natural Products Estee Lauder Companies September 24, 2009 SCC Education Day R&D Capabilities Product development assistance (scale up from a bench top process) Phytochemical analysis (to determine marker compound) Full analytical support (method development) Requirements ISO 9001:2000 certified (cGMP) Heavy Metal and Pesticide analysis Safety test for topical application Sustainable Biomass (wild craft, cultivation) Certified Organic (NOP, EcoCert, IFOAM) Challenges Lot to lot consistency: What are we really getting? Microbial issues 10,000 cfu for dietary supplement vs 100 cfu for cosmetic Heavy metal and Pesticide analysis – due to the pollution in soil and water Regulatory (REACH) and Safety (Topical) Patents- not patent protected Converting an ingestible for topical use (need to define biological activity) Cosmetic Products Are designed not only to moisturize the skin and reduce the visibility of lines and wrinkles… But also to protect the skin from the damages caused by environmental exposure. Allergen UV Pain Irritant COX2 IL -1α AA PGE2 KC MN TNF PLA 2 ILIL --1αααα Collagenases MMP -1 PLA 2 Nitric oxide (NO) IL-12 TC PLA 2 PL AA NO PMN FB NO TC TC CAMs Blood flow PG’s EC Ozone UVB , ozone , smoke PL A UVB arachadonic2 acid O O 3 3 cox 2 O cigarette smoke PGE 2 3 O3 Epidermis Basement membrane PGE 2 PGE 2 neutrophil collagen capillary Dermis collagenase elastase neutrophil macrophage vasodilation Control of Inflammatory Reactions Must take place at the very beginning of the cascade of reactions. Inhibition of Phospholipase A2 will result in the control of the release of Arachidonic Acid, a major inflammatory mediator. Additional anti-inflammatories will subsequently modulate the other steps of the cascade. Insult (UV exposure, oxidative stress) Poria Cocos Activation of Phospholipase A2 Resveratrol, hops Release of Arachidonic acid Centaurium Cyclo-oxygenase Lipoxygenase Release of Release of Leukotrienes Prostaglandins Cytokines (ILI,TNF ∝) Recruitment of Leukocyte cells Transport of Leukocytes by blood Transfer to site of injury (chemotaxis) Second oxidative burst and Protease (collagenase, elastase, tryptase) Rosemary, Citri reticulatae peel Grape seed extract Premature Aging The Trend Toward Natural Your consumers are watching and learning! Consumer awareness is at an all time high. Consumers want healthy products - and the industry is responding. Natural ingredients and healthy lifestyle are attractive to consumers. Morus spp. Over 80% of the world's population uses plants as their primary medicines 500,000 10,000 plant substances species per species What makes plants so efficacious ? multi-taskers inpired by many ; years of folklore validated by science biodegradable and sustainable High performance ingredients For high performance skin care Finding new ingredients Partnership with consultants and suppliers all over the world Strategic alliance with academic research Rhodiola rosea OH HO O O O HO OH O HO OH OH HO O HO O OH OH rosavin salidroside Treatment of skin cells with Rhodiola extract enhanced the ability of the cells to survive after exposure to an external stress (UV exposure) 100 80 60 untreated control 40 % survival Rhodiola rosea 20 0 30 60 90 120 150 UVB dose (mJ/cm 2) Rhodiola rosea Enhances Rebound from Stress 35 30 25 20 15 untreated control Rhodiola rosea 10 5 number of damaged cells 0 50 100 150 200 UVB dose (mJ/cm 2) After 24 hours the number of damaged cells was more then 2X lower in the Rhodiola treated skin model Cellular Damage (UVB, oxidative damage, inflammatory cytokines) ATP (energy depletion) DNA damage Mitochondrial DNA damage cancer (-)Physiological effects Animation 2 Animation 4 Rhodiola rosea Enhances Rebound from Stress Skin model stressed by simulated UV TREATED with No treatment Rhodiola rosea vs. Dark brown staining identifies heavily damaged skin cells. The removal of damage was much faster in Rhodiola treated tissue . Mimosa tenuiflora Mimosa bark extract with Flavonoids, tannins and saponins. Anti-collagenase: EC50 = 80 ug/ml Collagen synthesis: 31% at 0.0625 µg/ml and by 67% at 0.25 µg/ml, Anti-collagenase Activity of Tepescohuite EC50 = 80ug/ml 100.00 80.00 60.00 40.00 % inhibition 20.00 0.00 0 1.3 4 11.5 34 100 310 920 [tepescohuite] µg/ml Collagen Assay: Tepescohuite 12 10 8 6 Collagen (µg/ml) Collagen 4 2 0 Media 0.015625 0.0625 0.25 Dose (µg/ml) Langerhans Stratum cell corneum elastosis Mast melanocyte cell elastin collagen Photodamaged skin Young skin Old skin Degraded collagen, thickened elastin Loss of collagen & elastin Young cellsvs. Old cells A B Fragmented Fibrils In vitro cell proliferation as a function of age 20000 15000 2 10000 Cells/cm 5000 0 24 Yr 45 Yr 68 Yr In vitro collagen production as a function of age 0.14 0.12 0.1 0.08 0.06 0.04 Collagen ProducedCollagen (ng) per Cell 0.02 0 24 Yr 45 Yr What makes a wrinkle Constant folding & unfolding of skin creates a weak point: the micro- tear. Physical pressure on cells activates inflammatory reactions lead to premature degradation of collagen by collagenase Stress induces damages to skin cells result in further loss of skin integrity – Wrinkle formation WrinkleWrinkle animationanimation (4)(4) skinskin foldingfolding developmentdevelopment ofof inflammatoryinflammatory reactionreaction Anti-Age: Dermatological Approach Peels and lasers trigger cell’s natural defense mechanism. Skin’s repair process activated by damaged skin cells. BENEFIT: improves skin Inflammatory reaction degrades collagen & elastin. SIDE EFFECT: ages skin Anti-Age: A Natural Approach Prevent : control collagenase, elastase and GAGase degrading enzyme to prevent wrinkle formation. Restore : collagen synthesis to accelerate “Healing” of skin Repair : help skin cells rebound from stress and prevent premature aging Collagen Fragmentation by Collagenase Healthy skin Aging/stressed skin Collagen Collagenase Endogenous collagenase inhibitors ANIMATIONANIMATION 5b5b collagenasecollagenase inhibitorinhibitor ANIMATION 3 treatment of whole skin; fragments go to fibroblasts, activate collagen production. Anti-Age: Wrinkle Prevent premature destruction of key dermal components Treatment: Anti-collagenase (Sigesbeckia, Tepescohuite) Anti-elastase (Boswellia, White Birch) Anti-GAGase (Silymarin, Centaurium) Collagen synthesis (Tepescohuite) Result: Treatment of skin, most damaged by constant folding / unfolding. Matricaria recutita flower Blue chamomile oil with confirmed: 5-LO inhibitory activity: EC50 = 50 ug/ml Anti-chemotaxis: 97% inh. At 0.05% LPO: EC50 = 0.003% German Chamomile 5-Lipoxygenase assay SCOPE To analyse potential inhibitors of potato 5-lipoxygenase (5-LO). PRINCIPLE 5-lipoxygenase converts the substrate linoleic acid (diene 1-4) to a conjugated diene product (diene 1-3), which can be detected at 234nm. A decrease in the absorbance signal is indicative for an increased anti-5LO activity. MATERIAL Normal lab equipment 96 well half area plates with UV transparent bottom (Costar, 3679) 8 channel multi pipette (20-200µl) Spectrophotometer REAGENTS H2O distilled Potassium dihydrogen phosphate (Acros, #205925000) Tween-20 (Janssen Chimica, #2333656) Dimethylsulfoxide (Acros, #12779) Potato 5-lipoxygenase (Cayman, #60400) Linoleic acid 99% (Acros, #215040050) Nordihydroguaiaretic acid from Larrea divaricata (creosote bush) (approx. 95%)(reference inhibitor - NDGA) (Sigma, N1144) 5-Lipoxygenase Inhibition IC 50 ~50µg/ml 120.00 100.00 80.00 60.00 40.00 % Initial 5LO activity 20.00 0.00 0 10 20 30 40 50 60 70 80 90 [Blue chamomile oil organic (Hashem Brothers, #4905)] (µg/ml) Chemotaxis Assay: Blue Chamomile Oil Organic (BRD 4905) 120 100 80 60 PMNs/HPF 40 97% inhibition at 0.05% 20 0 Ctrl Caff .5% #4905 .005% #4905 .05% LPO (Anti-oxidant) 0.003 0.003 0.002 0.002 0.001 0.001 0.000 30 min 120min Conclusions The development of new ingredients from plant extracts involves many steps which can ultimately provide great benefits in cosmetics. Despite the cost involved in such development, we believe that active ingredients from natural sources provides the cosmetic industry with a quasi infinite source of ingredients which we are aiming at developing in the future. Conclusion Opportunity not a Headache Carefully choose your partner Know what you are working with (Standardize extract).
Load more

Recommended publications
  • Sigesbeckia Orientalis Click on Images to Enlarge
    Species information Abo ut Reso urces Hom e A B C D E F G H I J K L M N O P Q R S T U V W X Y Z Sigesbeckia orientalis Click on images to enlarge Family Asteraceae Scientific Name Sigesbeckia orientalis L. Linnaeus, C. von (1753) Species Plantarum 2: 900. Type: Habitat in China, Media ad pagos. Common name Flower head. Copyright Barry Jago Indian Weed; Farmer's Lice Weed * Stem Usually flowers and fruits as a shrub about 1 m tall but also flowers when smaller. Leaves Habit, leaves and flowers. Copyright CSIRO Leaves variable, leaf blades up to 6 x 3-3.5 cm, lower surface clothed in numerous small yellow glands. Lateral veins forming loops well inside the blade margin. Both the upper and lower surfaces of the leaf blade hairy. Twigs marked by scars resembling stipular scars. Stems hollow, pith absent. Flowers Flowers sessile in heads, about 15 flowers per head. Each head of flowers usually contains some female flowers, each consisting of a +/- 3-lobed perianth, and some hermaphrodite flowers with a 5-lobed perianth tube enclosing five anthers fused into a tube. Each head subtended by an involucre of about five green spathulate bracts which are clothed in sticky glands. Outer bracts about 5-10 x 0.5 mm. Corolla orange or yellow in the upper half but green in the lower half. Ovary glabrous. Fruit Scale bar 10mm. Copyright CSIRO Disk achenes 4-angular, about 2-5 mm long. Ray achenes clasped by persistent glandular hairy bracts.
    [Show full text]
  • Chromosome Numbers in Compositae, XII: Heliantheae
    SMITHSONIAN CONTRIBUTIONS TO BOTANY 0 NCTMBER 52 Chromosome Numbers in Compositae, XII: Heliantheae Harold Robinson, A. Michael Powell, Robert M. King, andJames F. Weedin SMITHSONIAN INSTITUTION PRESS City of Washington 1981 ABSTRACT Robinson, Harold, A. Michael Powell, Robert M. King, and James F. Weedin. Chromosome Numbers in Compositae, XII: Heliantheae. Smithsonian Contri- butions to Botany, number 52, 28 pages, 3 tables, 1981.-Chromosome reports are provided for 145 populations, including first reports for 33 species and three genera, Garcilassa, Riencourtia, and Helianthopsis. Chromosome numbers are arranged according to Robinson’s recently broadened concept of the Heliantheae, with citations for 212 of the ca. 265 genera and 32 of the 35 subtribes. Diverse elements, including the Ambrosieae, typical Heliantheae, most Helenieae, the Tegeteae, and genera such as Arnica from the Senecioneae, are seen to share a specialized cytological history involving polyploid ancestry. The authors disagree with one another regarding the point at which such polyploidy occurred and on whether subtribes lacking higher numbers, such as the Galinsoginae, share the polyploid ancestry. Numerous examples of aneuploid decrease, secondary polyploidy, and some secondary aneuploid decreases are cited. The Marshalliinae are considered remote from other subtribes and close to the Inuleae. Evidence from related tribes favors an ultimate base of X = 10 for the Heliantheae and at least the subfamily As teroideae. OFFICIALPUBLICATION DATE is handstamped in a limited number of initial copies and is recorded in the Institution’s annual report, Smithsonian Year. SERIESCOVER DESIGN: Leaf clearing from the katsura tree Cercidiphyllumjaponicum Siebold and Zuccarini. Library of Congress Cataloging in Publication Data Main entry under title: Chromosome numbers in Compositae, XII.
    [Show full text]
  • Genetic Diversity, Phylogenetics and Molecular Systematics of Guizotia Cass
    Genetic Diversity, Phylogenetics and Molecular Systematics of Guizotia Cass. (Asteraceae) Mulatu Geleta Faculty of Landscape Planning, Horticulture and Agricultural Science Department of Plant Protection Biology Alnarp Doctoral thesis Swedish University of Agricultural Sciences Alnarp 2007 Acta Universitatis Agriculturae Sueciae 2007:27 ISSN 1652-6880 ISBN 978-91-576-7326-8 © 2007 Mulatu Geleta, Alnarp Tryck: SLU Service/Repro, Alnarp 2007 Abstract Geleta, M. 2007. Genetic diversity, phylogenetics and molecular systematics of Guizotia Cass. (Asteraceae). Doctoral dissertation. ISSN 1652-6880, ISBN 978-91-576-7326-8. The genus Guizotia belongs to the tribe Heliantheae in the family Asteraceae. It has been placed under different subtribes. The genus has its center of origin, distribution and genetic diversity in Ethiopia, where G. abyssinica (niger) has been domesticated. Amplified Fragment Length Polymorphism (AFLP), Random Amplified Polymorphic DNA (RAPD) and DNA sequencing were applied to study the genetic diversity, phylogenetics, and molecular systematics of this genus. A large number of niger populations, representing all regions in Ethiopia where this crop is grown, was investigated using AFLP and RAPD molecular marker techniques. The extent of genetic variation in niger is distributed throughout its growing regions, regardless of the extent and altitude of cultivation. Despite the fact that most of the variation was within populations, significant population differentiation was obtained (AMOVA; P < 0.001) in all guizotias. It is concluded that both G. abyssinica and its wild and/or weedy relatives have wide genetic bases that need to be conserved and utilized for the improvement of G. abyssinica. Further collection of niger germplasm and exploration and conservation of highly localized guizotias are recommended.
    [Show full text]
  • Leonuri Cardiacae Herba
    16 September 2010 EMA/HMPC/127429/2010 Committee on Herbal Medicinal Products (HMPC) List of references supporting the assessment of Leonurus cardiaca L., herba Final The Agency acknowledges that copies of the underlying works used to produce this monograph were provided for research only with exclusion of any commercial purpose. Abascal K, Yarnell E (2004) Nervine herbs for treating anxiety. Alternative & Complementary Therapies (England); 10:309-315 Abebe W (2002) Herbal medication: Potential for adverse interactions with analgesic drugs. Journal of Clinical Pharmacy and Therapeutics; 27:391-401 Agnihotri VK, ElSohly HN, Smillie TJ, Khan IA, Walker LA (2008) New Labdane Diterpenes from Leonurus cardiaca. Planta Med; 74:1288-1290 Ahmed F, Islam MA, Rahman MM (2006) Antibacterial activity of Leonurus sibiricus aerial parts. Fitoterapia; 77:316-317 Ahmed F, Islam MA, Choudhuri MSK (2005) Central nervous system depressant activity of Leonurus sibiricus. Nigerian Journal of Natural Products and Medicine; 9:35-37 Aizetmüller K, Tsevegüren N (1998) Phlomic acid in Lamioidae seed oils. Lamiales Newsletter; 6:13-16 Ali MS, Ibrahim SA, Jalil S, Choudhary MI (2007) Ursolic acid: A potent inhibitor of superoxides produced in the cellular system. Phytotherapy Res; 21:558-561 Almeida LFR, Delachiave MEA, Marques MOM (2005) Composição do óleo essencial de rubim (Leonurus sibiricus L. - Lamiaceae). [Composition of the essential oil of rubim (Leonurus sibiricus L. - Lamiaceae).] Revista Brasileira de Plantas Medicinais; 8:35-38 American Botanical Council (2008) Herbs for potential adjunct treatment of thyroid disease. A review of botanical preparations for hypo- and hyperthyroidism, thyroid nodules, and thyroid cancer. HerbalGram; 79:52-65 7 Westferry Circus ● Canary Wharf ● London E14 4HB ● United Kingdom Telephone +44 (0)20 7418 8400 Facsimile +44 (0)20 7523 7051 E-mail [email protected] Website www.ema.europa.eu An agency of the European Union © European Medicines Agency, 2010.
    [Show full text]
  • Supplement to the Systematics of the Heliantheae (Compositae)
    Boletín de la Sociedad Argentina de Botánica Volumen XIX, N? 1-2 (Julio 1980), págs. 25-32 SUPPLEMENT TO THE SYSTEMATICS OF THE HELIANTHEAE (COMPOSITAE) Por TOD F. STUESSY2 Since the completion of a revised subtribal classification of the tribe Heliantheae (Stuessy, in press) for the Reading Symposium on the Compositae in July of 1975, a number of new papers have been published on the systematics of the tribe. This new informa¬ tions falls into four general categories: (1) speculations on relation¬ ships among the newly recognized subtribes; (2) addition of a new subtribe; (3) additions of new genera; and (4) new generic transfers. The purpose of this paper is to review these new developments and comment on their significance for the systematics of the Heliantheae. RELATIONSHIPS AMONG THE NEWLY RECOGNIZED SUBTRIBES An important new overview of relationships among the sub¬ tribes of the Heliantheae has been provided by Baagoe (1977) by use of ligule microcharacters. This contribution is especially valuable, because the manuscript on the Heliantheae for the Reading Sympo¬ sium (Stuessy, in press) was available to Dr. Baagoe during her study, and she was able to relate the new data to the subtribal clas¬ sification of the tribe. Many specific comments on relationships are contained in her paper, but this review will focus only on some of the broader implications. Before these are examined, however, a few cautions need to be raised. 1 It is a pleasure to dedicate this paper to Dr. Angel L. Cabrera, one of the outstanding synantherologists of the world, and the unqualified authority on South American Compositae.
    [Show full text]
  • Sigesbeckia Orientalis L
    Australian Tropical Rainforest Plants - Online edition Sigesbeckia orientalis L. Family: Asteraceae Linnaeus, C. von (1753) Species Plantarum 2: 900. Type: Habitat in China, Media ad pagos. Common name: Indian Weed; Farmer's Lice Stem Usually flowers and fruits as a shrub about 1 m tall but also flowers when smaller. Leaves Leaves variable, leaf blades up to 6 x 3-3.5 cm, lower surface clothed in numerous small yellow glands. Lateral veins forming loops well inside the blade margin. Both the upper and lower surfaces of the leaf blade hairy. Twigs marked by scars resembling stipular scars. Stems hollow, pith absent. Flowers Flowers sessile in heads, about 15 flowers per head. Each head of flowers usually contains some female flowers, each consisting of a +/- 3-lobed perianth, and some hermaphrodite flowers with a 5- lobed perianth tube enclosing five anthers fused into a tube. Each head subtended by an involucre Flower head. © Barry Jago of about five green spathulate bracts which are clothed in sticky glands. Outer bracts about 5-10 x 0.5 mm. Corolla orange or yellow in the upper half but green in the lower half. Ovary glabrous. Fruit Disk achenes 4-angular, about 2-5 mm long. Ray achenes clasped by persistent glandular hairy bracts. Seedlings Hypocotyl clothed in short erect hairs just visible with a lens. Stem above the cotyledons clothed in much longer erect white hairs. Cotyledons +/- orbicular, about 6-7 x 5-6 mm. First pair of leaves with toothed margins. At the tenth leaf stage: stem, terminal bud and both the upper and lower leaf blade surfaces clothed in white somewhat scabrous hairs.
    [Show full text]
  • The Naturalized Vascular Plants of Western Australia 1
    12 Plant Protection Quarterly Vol.19(1) 2004 Distribution in IBRA Regions Western Australia is divided into 26 The naturalized vascular plants of Western Australia natural regions (Figure 1) that are used for 1: Checklist, environmental weeds and distribution in bioregional planning. Weeds are unevenly distributed in these regions, generally IBRA regions those with the greatest amount of land disturbance and population have the high- Greg Keighery and Vanda Longman, Department of Conservation and Land est number of weeds (Table 4). For exam- Management, WA Wildlife Research Centre, PO Box 51, Wanneroo, Western ple in the tropical Kimberley, VB, which Australia 6946, Australia. contains the Ord irrigation area, the major cropping area, has the greatest number of weeds. However, the ‘weediest regions’ are the Swan Coastal Plain (801) and the Abstract naturalized, but are no longer considered adjacent Jarrah Forest (705) which contain There are 1233 naturalized vascular plant naturalized and those taxa recorded as the capital Perth, several other large towns taxa recorded for Western Australia, com- garden escapes. and most of the intensive horticulture of posed of 12 Ferns, 15 Gymnosperms, 345 A second paper will rank the impor- the State. Monocotyledons and 861 Dicotyledons. tance of environmental weeds in each Most of the desert has low numbers of Of these, 677 taxa (55%) are environmen- IBRA region. weeds, ranging from five recorded for the tal weeds, recorded from natural bush- Gibson Desert to 135 for the Carnarvon land areas. Another 94 taxa are listed as Results (containing the horticultural centre of semi-naturalized garden escapes. Most Total naturalized flora Carnarvon).
    [Show full text]
  • Sesquiterpene Lactone Potentiates the Immunomodulatory, Antiparasitic and Cardioprotective Effects on Anti-Trypanosoma Cruzi Specific Chemotherapy T
    International Immunopharmacology 77 (2019) 105961 Contents lists available at ScienceDirect International Immunopharmacology journal homepage: www.elsevier.com/locate/intimp Sesquiterpene lactone potentiates the immunomodulatory, antiparasitic and cardioprotective effects on anti-Trypanosoma cruzi specific chemotherapy T Elda Gonçalves-Santosa, Diego F. Vilas-Boasa, Lívia F. Diniza, Marcia P. Velosob, Ana L. Mazzetia, Maria R. Rodriguesb, Carla M. Oliveirab, Victor Hugo C. Fernandesd, Rômulo D. Novaesc, ⁎ Daniela A. Chagas-Paulad, Ivo S. Caldasa, a Institute of Biomedical Sciences, Department of Pathology and Parasitology, Federal University of Alfenas, 37130-001 Alfenas, MG, Brazil b Pharmaceutical Sciences Institute, Federal University of Alfenas, Alfenas 37130-001, MG, Brazil c Institute of Biomedical Sciences, Department of Structural Biology, Federal University of Alfenas, 37130-001 Alfenas, MG, Brazil d Chemistry Institute, Federal University of Alfenas, 37130-001 Alfenas, MG, Brazil ARTICLE INFO ABSTRACT Keywords: We investigated the immunomodulatory, antiparasitic and cardioprotective effects of a sesquiterpene lactone Chagas’s disease (SL) administered alone or combined with benznidazole (Bz), in a murine model of Chagas’ disease by in vitro Benznidazole and in vivo assays. Antiparasitic and cytotoxic potential of tagitinin C (SL) and Bz were tested in vitro against T. Infectious myocarditis cruzi epimastigotes and cardiomyocytes. Swiss mice challenged with T. cruzi were also treated for 20 days with Sesquiterpene lactone tagitinin C (10 mg/kg) alone and combined with Bz (100 mg/kg). Tagitinin C exhibited a higher antiparasitic Tagitinin C (IC50: 1.15 µM) and cytotoxic (CC50 at 6.54 µM) potential than Bz (IC50: 35.81 µM and CC50: 713.5 µM, re- spectively). When combined, these drugs presented an addictive interaction, determining complete suppression of parasitemia and parasitological cure in all infected mice (100%) compared to those receiving Bz alone (70%).
    [Show full text]
  • Germacrane-Type Sesquiterpenoids with Cytotoxic Activity From
    Bioorganic Chemistry 92 (2019) 103196 Contents lists available at ScienceDirect Bioorganic Chemistry journal homepage: www.elsevier.com/locate/bioorg Germacrane-type sesquiterpenoids with cytotoxic activity from Sigesbeckia T orientalis Na Liua, Cong Wua, Jin-hai Yua, Kong-kai Zhua, Mei-na Songa, Feng-ying Yanga, Run-liang Fenga, ⁎ Yu-ying Zhanga, Wen-qiang Changb, Hua Zhanga, a School of Biological Science and Technology, University of Jinan, 336 West Road of Nan Xinzhuang, Jinan 250022, China b Key Lab of Chemical Biology of Ministry of Education, Shandong University, 44 West Wenhua Road, Jinan 250012, China ARTICLE INFO ABSTRACT Keywords: Eleven new highly oxygenated germacrane-type sesquiterpenoids (1–11) and 16 known analogues (12–27) were Sigesbeckia orientalis isolated from the aerial parts of Sigesbeckia orientalis. Their structures, including absolute configurations, were Germacrane determined by comprehensive spectroscopic methods especially NMR and ECD analyses. Compounds 13, 21 and Sesquiterpenoids 23 possessing an 8-methacryloxy group showed stronger in vitro cytotoxicity against human A549 and MDA-MB- Cytotoxicity 231 cancer cell lines than other co-metabolites, with IC50 values ranging from 6.02 to 10.77 μM comparable to the positive control adriamycin. 1. Introduction against human cancer cells with IC50 values ranging from 0.9 to 33.3 μM [8,9]. However, to the best of our knowledge, most of the Sigesbeckia L. is a small genus of the plant family Compositae (also germacranolides isolated previously have not been biologically tested. known as Asteraceae) but distributed all over the Northern Hemisphere. In our continuing search for new bioactive molecules from medicinal Three species, S.
    [Show full text]
  • Classification, Diversity, and Distribution of Chilean Asteraceae
    Diversity and Distributions, (Diversity Distrib.) (2007) 13, 818–828 Blackwell Publishing Ltd BIODIVERSITY Classification, diversity, and distribution RESEARCH of Chilean Asteraceae: implications for biogeography and conservation Andrés Moreira-Muñoz1 and Mélica Muñoz-Schick2* 1Geographical Institute, University ABSTRACT Erlangen-Nürnberg, Kochstr. 4/4, 91054 This paper provides a synopsis of the Chilean Asteraceae genera according to the Erlangen, Germany, 2Museo Nacional de most recent classification. Asteraceae is the richest family within the native Chilean Historia Natural, Casilla 787, Santiago, Chile flora, with a total of 121 genera and c. 863 species, currently classified in 18 tribes. The genera are distributed along the whole latitudinal gradient in Chile, with a centre of richness at 33°–34° S. Almost one-third of the genera show small to medium-small ranges of distribution, while two-thirds have medium-large to large latitudinal ranges of distribution. Of the 115 mainland genera, 46% have their main distribution in the central Mediterranean zone between 27°–37° S. Also of the mainland genera, 53% occupy both coastal and Andean environments, while 33% can be considered as strictly Andean and 20% as strictly coastal genera. The biogeographical analysis of relationships allows the distinction of several floristic elements and generalized tracks: the most marked floristic element is the Neotropical, followed by the anti- tropical and the endemic element. The biogeographical analysis provides important insights into the origin and evolution of the Chilean Asteraceae flora. The presence of many localized and endemic taxa has direct conservation implications. Keywords *Correspondence: Mélica Muñoz-Schick, Museo Nacional de Historia Natural, Casilla 787, Compositae, phylogeny, phytogeography, panbiogeography, synopsis Chilean Santiago, Chile.
    [Show full text]
  • Lose the Plot: Cost-Effective Survey of the Peak Range, Central Queensland
    Lose the plot: cost-effective survey of the Peak Range, central Queensland. Don W. Butlera and Rod J. Fensham Queensland Herbarium, Environmental Protection Agency, Mt Coot-tha Botanic Gardens, Mt Coot-tha Road, Toowong, QLD, 4066 AUSTRALIA. aCorresponding author, email: [email protected] Abstract: The Peak Range (22˚ 28’ S; 147˚ 53’ E) is an archipelago of rocky peaks set in grassy basalt rolling-plains, east of Clermont in central Queensland. This report describes the flora and vegetation based on surveys of 26 peaks. The survey recorded all plant species encountered on traverses of distinct habitat zones, which included the ‘matrix’ adjacent to each peak. The method involved effort comparable to a general flora survey but provided sufficient information to also describe floristic association among peaks, broad habitat types, and contrast vegetation on the peaks with the surrounding landscape matrix. The flora of the Peak Range includes at least 507 native vascular plant species, representing 84 plant families. Exotic species are relatively few, with 36 species recorded, but can be quite prominent in some situations. The most abundant exotic plants are the grass Melinis repens and the forb Bidens bipinnata. Plant distribution patterns among peaks suggest three primary groups related to position within the range and geology. The Peak Range makes a substantial contribution to the botanical diversity of its region and harbours several endemic plants among a flora clearly distinct from that of the surrounding terrain. The distinctiveness of the range’s flora is due to two habitat components: dry rainforest patches reliant upon fire protection afforded by cliffs and scree, and; rocky summits and hillsides supporting xeric shrublands.
    [Show full text]
  • Alphabetical Index of Plant Families and Groups
    00(2). Kingdom plants index 27/8/11 11:59 Page li ALPHABETICAL INDEX OF PLANT FAMILIES AND GROUPS 1 Acanthaceae 10 Andrographis. Hygrophila. Justicia adhatoda. 2 Acoraceae 1 Acorus calamus. 3 Actinidiaceae 1 Actinidia deliciosa (kiwi). 4 Agavaceae 5 Agave. Chlorogalum. Chlorophytum. Yucca. 5 Aizoaceae 3 Lampranthus. Mesembryanthum. Sceletium tortuosum. 6 Alismatales 3 Alisma plantago. Potamogeton. Sagittaria sagittaefolia. 7 Alliaceae/Agapanthaceae 10 Allium cepa. Allium sativum. Agapanthus. 8 Amaranthaceae/ 23 Achyranthes. Chenopodium. SalsolaLtd tragus. Chenopodiaceae 9 Amaryllidaceae 6 Amaryllis. Boophone. Galanthus. Narcissus. 10 Anacardiaceae 20 Anacardium. Comocladia. Mangifera. Pistacia. Rhus. Schinus. 11 Annonaceae 6 Annona. Asimina. Cananga. Guatteria. Malmea. Xylopia. 12 Apiaceae (Umbelliferae) 69 Aethusa. Ammi. Angelica. Apium. Asaf. Cicuta. Conium. Coriandrum. Ferula. Heracleum. Hydrocotyle. Oenanthe. Petroselinum. Peucedanum. Phellandrium. Sium. Sumbul. Zizia. 13 Apocynaceae 57 Alstonia.Books Apocynum. Asclepias. Calotropis. (incl. Asclepiadaceae Cundurango. Gelsemium. Gymnema. and Gelsemiaceae) Oleander. Quebracho. Rauvolfia. Reserpinum. Strophanthus. Tabernanthe iboga. Vinca. Vincetoxicum. 14 Aquifoliaceae 5 Ilex (holly). 15 Araceae (Aroids) 15 Amorphophallus. Arisaema. Arum. Caladium. Calla. Ictodes. Lemna. Monstera. Symplocarpus. Zantedeschia. 16 Araliaceae 11 Aralia. Ginseng. Hedera (ivy). Oplopanax. Panax. 17 Arecaceae (Palms) 9 Areca. Cocos. Elaeis. Euterpe. Nux absurda. SaltirePhoenix. Sabal. 18 Aristolochiaceae 8 Aristolochia. Asarum. 19 Asparagaceae 10 Asparagus. Convallaria. Cordyline. Dracaena. ©(incl. Laxmanniaceae Polygonatum. Ruscus. Sansevieria. and Ruscaceae) 20 Asteraceae (Compositae) 154 remedies – divided in 5 tribes 20.1 Asteraceae – Anthemidae 28 Abrot. Absinth. Artemisia. Cham. Cina. Millefolium. Tanacetum. 20.2 Asteraceae – Cardueae 18 Carduus. Centaurea. Cirsium. Cynara. Lappa. Onopordon. li 00(2). Kingdom plants index 27/8/11 11:59 Page lii 20.3 Asteraceae – Lactuceae 14 Cichorium. Hieracium. Lactuca. Nabalus.
    [Show full text]