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Bone Growth & Remodeling

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Bone Growth & Remodeling Bone Growth & Remodeling Tools for Cell Biology Research™ Tools for Cell Biology Research™ Bone Growth and Remodeling The skeletal system provides a tion. The dynamic processes of bone structural framework for the body, deposition and resorption continu- facilitating movement and protect- ously remodel bones, and this balance ing internal organs, as well as serving is crucial for maintenance of bone as a reservoir for inorganic salts and size, shape, and integrity. Disruptions cells of the immune system. Many cell of bone homeostasis accompany types and signaling pathways impact disorders that include osteoporosis, bone growth and remodeling: chon- arthritis, and many heritable skeletal drocytes and osteoblasts derived diseases. from bone marrow mesenchymal R&D Systems offers an array of tools for stem cells, osteoclasts of hematopoi- the study of skeletal biology, including etic origins, and hormones and other proteins, antibodies, and ELISAs, along neuromodulators that help regulate with kits for cell selection and apoptosis. the balance of osteoblastic bone for- Visit www.RnDSystems.com/go/Bone mation and osteoclastic bone resorp- Aggrecan Expression in Human Cartilage. Ag- Alkaline Phosphatase Expression in Human grecan was detected in paraffin-embedded human Kidney. Alkaline phosphatase was detected in hu- cartilage tissue sections using anti-human aggrecan man kidney sections using anti-alkaline phospha- polyclonal antibody (Catalog # AF1220). Tissues were tase monoclonal antibody (Catalog # MAB1448). stained using R&D Systems anti-goat HRP-DAB Cell Sections were stained using Rhodamine Red™-conju- and Tissue Staining Kit (Catalog # CTS008; brown) and gated anti-mouse secondary antibody (red) and coun- counterstained with hematoxylin (blue). terstained using FluoroNissl™ (green). 2 Please visit our website, www.RnDSystems.com, for other related products. For Research Use Only. Not for use in diagnostic procedures. www.RnDSystems.com Bone Growth & Remodeling Products off ered by R&D Systems Molecule Antibodies Proteins ELISAs & Kits Activin A H M R H M R H Activin RIA/ALK-2 H H Activin RIB/ALK-4 H M H M Activin RIIA, B HH Activin RIIB HH M Aggrecan HH Akt1 H M R H H M R ALK-1 H M H M ALK-7 RR Alkaline Phosphatase H M R H M Angiopoietin-1 HHH Annexin A2 H M R Annexin V HH AP-1/c-jun H APC H Apoptosis-related Products, see www.RnDSystems.com/go/Apoptosis for a complete listing Axin-1 H M R Bcl-2 H M H H β-Catenin H M R X H Biglycan HH BMP-2 H Z H Z H M R BMP-2/BMP-7 heterodimer H BMP-3 HH BMP-3b/GDF-10 HH BMP-4 H ZH ZH BMP-5 HHH BMP-6 HHH BMP-7 HHH BMP-8 HH BMP-9 HH BMP-10 HH BMP-15/GDF-9B H BMPR-IA/ALK-3 HH M BMPR-IB/ALK-6 H M H M Abbreviation key: B: Bovine Ca: Canine Ch: Chicken CR: Cotton Rat D: Drosophila E: Equine F: Feline 3 H: Human M: Mouse Ms: Multi-species P: Porcine Pr: Primate R: Rat V: Viral X: Xenopus Z: Zebrafi sh Tools for Cell Biology Research™ Reduced Non-Reduced M.W. (kDa) 29 BMP-2/7 Heterodimer 21 18.4 BMP-7 12.4 BMP-2 BMP-8 Expression in Human Osteosarcoma. BMP- 8 was detected in paraffin-embedded human osteo- sarcoma tissue sections using anti-human BMP-8 Recombinant BMP Heterodimer. R&D Systems polyclonal antibody (Catalog # AF1073). Tissues were recombinant human BMP-2/BMP-7 protein (Catalog # stained using R&D Systems anti-goat HRP-DAB Cell 3229-BM) is a refolded disulfide-linked heterodimer. and Tissue Staining Kit (Catalog # CTS008; brown) and Under reducing conditions the heterodimer separates counterstained with hematoxylin (blue). into monomers as revealed by SDS-PAGE. Bone Growth & Remodeling Products off ered by R&D Systems Molecule Antibodies Proteins ELISAs & Kits BMPR-II HH BMP inhibitors, see the table on page 15 cAMP Ms Ms Cannabinoid R1 H CART H N-Cadherin HH Cadherin-6 HH Cadherin-11 HH Calcineurin H Calcitonin R H Carbonic Anhydrase II HH Cathepsin S HHH CBFA1/RUNX2 H CCL6/C10 MM CCL8/MCP-2 H M H H CCL12/MCP-5 MMM CD44/OPN Receptor HH CNP H Collagen I R B Ms 4 Please visit our website, www.RnDSystems.com, for other related products not listed here. For Research Use Only. Not for use in diagnostic procedures. www.RnDSystems.com 70 60 50 40 30 20 Relative Cell Number Cell Relative 10 0 100 101 102 103 104 BMPR-II CBFA1/RUNX2 Expression in MC-3T3 cells. CBFA1/ RUNX2 was detected in MC-3T3 pre-osteoblast cells Detection of BMPR-II in MG-63 cells using Flow using anti-human CBFA1/RUNX2 polyclonal anti- Cytometry. MG-63 osteosarcoma cells were stained body (Catalog # AF2006). Cells were stained using with anti-human BMPR-II polyclonal antibody Rhodamine Red-conjugated anti-goat secondary (Catalog # AF811, filled histogram) or goat IgG antibody. control (Catalog # AB108, open histogram) followed by APC-conjugated anti-goat secondary antibody (Catalog # F0108). Bone Growth & Remodeling Products off ered by R&D Systems Molecule Antibodies Proteins ELISAs & Kits Collagen II M COMP/Thrombospondin-5 HH Connexin 43 H M R Pr COX-1 H COX-2 H M CTGF/CCN2 H CXCL8/IL-8 H Ca F P H P Ca F H P Ca CXCL12/SDF-1 H M H M F Pr H M CXCR4 H M F Decapentaplegic DD Decorin H M H M H M Dishevelled H Dkk-1 H M H M R H M Dkk-2 MM DMP-1 MH DSPG3 H M EGF H M R H M R H M EGF R H MH MH Endoglin/CD105 H MH MH Abbreviation key: B: Bovine Ca: Canine Ch: Chicken CR: Cotton Rat D: Drosophila E: Equine F: Feline 5 H: Human M: Mouse Ms: Multi-species P: Porcine Pr: Primate R: Rat V: Viral X: Xenopus Z: Zebrafi sh Tools for Cell Biology Research™ EphA4 Expression in Rat Cartilage. EphA4 was de- Galectin-3 Expression in Embryonic Mouse Ribs. tected in a cross-section of rib cartilage primordia in Galectin-3 was detected in a cryostat tissue section E15 rat embryos using anti-mouse EphA4 polyclonal of mouse embryonic (15 d.p.c.) ribs using anti-mouse antibody (Catalog # AF641). Tissue was stained using Galectin-3 polyclonal antibody (Catalog # AF1197). Cy™3-conjugated anti-goat secondary antibody. Tissues were stained using the anti-goat HRP-DAB Cell and Tissue Staining Kit (Catalog # CTS008; brown) and counterstained with hematoxylin (blue). Bone Growth & Remodeling Products off ered by R&D Systems Molecule Antibodies Proteins ELISAs & Kits Endorepellin/Perlecan H M Endothelin-1 HH EphA4 MM EphB4 H MH MH Ephrin-B1 MM ERK H M R H H M R E-Selectin H M R H M R H M Estrogen R H Exostosin-like (EXT) H M Extracellular Matrix Components, see www.RnDSystems.com/go/ECM for a complete listing Fas Ligand H M R H M R H M FGF acidic H BH BH FGF basic H B H M R B H FGF-7/KGF H Ca H Ca H FGF-9 HHH FGF-23 H M H FGF R1 HH FGF R2 H MH MH FGF R3 H MH MH 6 Please visit our website, www.RnDSystems.com, for other related products not listed here. For Research Use Only. Not for use in diagnostic procedures. www.RnDSystems.com EphB1 Expression in Rat Cartilage. EphB1 was de- Gremlin Expression in Embryonic Mouse Ribs. tected in cartilage primordium of phalangeal bones in Gremlin was detected in a cryostat cross-section of rat embryos using anti-rat EphB1 polyclonal antibody mouse embryonic (15 d.p.c.) ribs using anti-mouse (Catalog # AF542). Tissue was stained using Cy3-con- Gremlin polyclonal antibody (Catalog # AF956). Tis- jugated anti-goat secondary antibody. sues were stained using R&D Systems anti-goat HRP- DAB Cell and Tissue Staining Kit (Catalog # CTS008; brown) and counterstained with hematoxylin (blue). See BMP Inhibitors table, page 15 for a product listing. Bone Growth & Remodeling Products off ered by R&D Systems Molecule Antibodies Proteins ELISAs & Kits FGF R4 H M H Fibronectin HH B Frizzled-1 H M M Galectin-3 H MH MM G-CSF H M H M H M G-CSF Rα H H GDF-5 MM GDF-6 M GDF-7 MM GDF-9 M GLI-1 H M Glypican-3 HH GM-CSF H M R Ca F P H M R Ca F P H M R F GM-CSF Rα H gp130/OSM R H MH MH Growth Hormone H R H H Growth Hormone R H M R H M R GSK-3 H M R H H M R HIF-1α H M R H M Abbreviation key: B: Bovine Ca: Canine Ch: Chicken CR: Cotton Rat D: Drosophila E: Equine F: Feline 7 H: Human M: Mouse Ms: Multi-species P: Porcine Pr: Primate R: Rat V: Viral X: Xenopus Z: Zebrafi sh Tools for Cell Biology Research™ Human Mesenchymal Stem Cell Functional Identifi cation Kit Mesenchymal Stem Cells (MSCs) Chondrogenic Differentiation Medium Osteogenic Adipogenic Differentiation Differentiation Medium Medium Culture Culture Culture 7 - 21 days 14 - 21 days 14 - 21 days A C E Chrondrocytes derived from MSCs differentiated in vitro with Chon- drogenic Supplement and identi- fied using R&D Systems anti-human Aggrecan polyclonal antibody (E). B D Adipocytes derived from MSCs Osteocytes derived from MSCs dif- differentiated in vitro with Adipo- ferentiated in vitro with Osteogenic genic Supplement and identified supplement and identified by (C) by (A) Oil Red staining and (B) using Alzarian red staining and (D) using R&D Systems anti-mouse FABP-4 R&D Systems anti-human Osteocal- polyclonal antibody.
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