Industrial Propagation by Rooted Cuttings of Mature Selected Clones of Hevea Brasiliensis

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Industrial Propagation by Rooted Cuttings of Mature Selected Clones of Hevea Brasiliensis BOIS ET FORÊTS DES TROPIQUES, 2013, N° 317 (3) PROPAGATION D’HÉVÉA / LE POINT SUR… 51 Industrial propagation by rooted cuttings of mature selected clones of Hevea brasiliensis Aurélien Masson Jean-Marc Julien Luc Boedt SoGB, SOCFIN group estate 01BP365 San Pedro Côte d’Ivoire Mass production by rooted cuttings of mature selected clones of Hevea brasiliensis in SoGB nursery facilities. Photograph A. Masson. BOIS ET FORÊTS DES TROPIQUES, 2013, N° 317 (3) 52 FOCUS / RUBBER TREE PROPAGATION A. Masson, J.-M. Julien, L. Boedt RÉSUMÉ ABSTRACT RESUMEN MULTIPLICATION INDUSTRIELLE PAR INDUSTRIAL PROPAGATION BY ROOTED PROPAGACIÓN INDUSTRIAL BOUTURAGE DE CLONES MATURES CUTTINGS OF MATURE SELECTED CLONES POR ESTAQUILLAS DE CLONES MADUROS D’HEVEA BRASILIENSIS OF HEVEA BRASILIENSIS DE HEVEA BRASILIENSIS L’importance de l'hévéa (caoutchouc), Hevea The importance of Hevea brasiliensis (rubber La importancia de la hevea (árbol del cau- brasiliensis, en tant que culture de rente ne tree) as a cash crop keeps increasing war- cho), Hevea brasiliensis, como cultivo cesse d'augmenter justifiant de s'intéresser ranting the development of new and more comercial no cesa de aumentar, lo que justi- à de nouvelles techniques de clonage plus efficient techniques than the bud-grafting fica el interés de nuevas técnicas más efi- efficaces que le greffage (écussonnage) tradi- traditionally used for mass producing supe- cientes que el injerto (en escudo) tradicio- tionnellement utilisé pour la production rior planting material. In vitro production of nalmente empleado para la producción industrielle de matériel de plantation de qua- rubber trees by somatic embryogenesis and industrial de material de cultivo de calidad lité supérieure. Les bonnes performances sur the good field performance (fast growth, superior. El buen desempeño en el campo le terrain (croissance rapide, haut rende- high yield) of the resulting emblings have (crecimiento rápido y alto rendimiento) de ment) des hévéas produits par embryoge- been reported for years, but so far only on an las heveas producidas por embriogénesis nèse somatique n'ont été constatées jusqu'à experimental scale. In vitro mass production somática, llevan años observándose, pero présent qu'à l'échelle expérimentale. La pro- of self-rooted rubber clones by somatic hasta ahora sólo a escala experimental. La pagation de masse in vitro par embryoge- embryogenesis or microcuttings is hindered propagación masiva in vitro por embriogéne- nèse somatique ou microbouturage de by a lack of responsiveness of most of the sis somática o microestaquillas de clones de clones d'hévéas sur leurs propres racines selected genotypes and by prohibitive pro- hevea de sus propias raíces, está obstaculi- reste pénalisée par un manque de réactivité duction costs. Given this situation, macro zada por falta de reacción positiva de parte de la plupart des génotypes sélectionnés et propagation by rooted cuttings following in de la mayoría de los genotipos selecciona- par des coûts de production prohibitifs. Face vitro propagation of mature selected clones dos y por costos de producción prohibitivos. à cette situation, la propagation par boutu- was attempted by the SoGB estate in Côte Ante esta situación, la propagación de esta- rage de clones matures sélectionnés issus de d'Ivoire as a possible alternative to in vitro quillas de clones maduros seleccionados micropropagation in vitro a été tentée par la techniques only. Two industrial mature procediendo de micopropagación in vitro, SoGB en Côte d’Ivoire comme une alternative clones, clone A (70yrs-old) and clone B ha sido llevada a cabo por la compañía possible à l’utilisation exclusive des tech- (53yrs-old), were first rejuvenated in vitro by SOGB de Côte d'Ivoire, en tanto que posible niques in vitro. Les deux clones matures somatic embryogenesis then micropropa- alternativa al uso de técnicas exclusiva- industriels, A (70 ans) et B (53 ans), ont gated in greater numbers by microcuttings. mente in vitro. Los dos clones maduros d’abord été rajeunis in vitro par embryoge- After acclimatization, these in vitro-rooted industriales, A (70 años de edad) y B nèse somatique puis micropropagés en plus microcuttings were potted in individual con- (53 años de edad), fueron primero rejuvene- grand nombre par microbouturage. Après tainers to be managed intensively as stock cidos in vitro por embriogénesis somática y acclimatation, les microboutures enracinées plants to produce macro cuttings. After luego micropropagados en mayor número in vitro ont été rempotées dans des pots indi- 3 weeks under suitable rooting conditions in por microestaquillas. Una vez aclimatadas, viduels pour être gérées de manière intensive the nursery, rooting rates of 74.6% las microestaquillas enraízadas in vitro se comme pieds-mères destinés au bouturage. (1203/1613) for clone A and 76.5% trasplantaron en macetas individuales para Après 3 semaines en conditions horticoles (198/259) for clone B macrocuttings were su manejo intensivo como plantas madres adéquates, les taux d’enracinement obtenus obtained. The cuttings produced vigorous destinadas a producir estaquillas. Al cabo pour les boutures des clones A et B ont été and taproot-like adventitious roots. After de tres semanas en condiciones adecuadas respectivement de 74,6 % (1203/1613) et successful acclimatization, all the rooted de cultivo, las tasas de enraizamiento logra- 76,5 % (198/259). Les racines adventices cuttings developed true-to-type and das fueron: 74,6 % (1203/1613) para el néoformées étaient généralement vigou- 4 months later reached a sufficient height of clon A y 76,5 % (198 /259) para el B. Las raí- reuses. A l’issue d’une phase d'acclimatation 25-30 cm to be field planted. In addition to ces adventicias neoformadas resultaron réussie, les boutures se sont développées de higher field vigor and true-to-typeness, cut- generalmente vigorosas. Tras el éxito de su façon conforme pour atteindre 4 mois plus ting-derived rubber tree clones can be pro- aclimatación, todas las estaquillas tuvieron tard une hauteur de 25-30 cm suffisante duced more quickly, on less nursery space at un desarrollo favorable y cuatro meses des- pour être plantées au champ. En sus d’une lower cost and in easier working conditions pués alcanzaron una altura de 25-30 centí- plus grande vigueur et conformité sur le ter- than traditionally bud-grafted plants. Further metros, lo suficiente para su trasplante en rain, les clones issus de bouturage peuvent investigations are underway for assessing at campo. Además de mayor vigor y mejor con- être produits plus rapidement, sur des sur- the field level the comparative advantages of formación en plantación, los clones reprodu- faces plus réduites à moindre coût et dans rooted cuttings versus grafted clonal off- cidos por estaquillas pueden multiplicarse des conditions de travail plus faciles par rap- spring with respect to traits of major eco- en vivero con mayor rapidez, menor espacio, port aux plantes issus d’écussonnage. Des nomic importance such as latex yield. fáciles condiciones de trabajo y costos infe- analyses en cours devraient permettre d'éta- riores a lo que requieren las plantas proce- blir les avantages comparatifs des boutures Keywords: Hevea brasiliensis, somatic dentes de injerto. Estudios en curso debe- par rapport aux plants écussonnés en ce qui embryogenesis, rooted cuttings, clone, rian poder establecer ventajas comparativas concerne d’autres caractères à fort impact rejuvenation, mass production, stock plant, de plantas procedentes de estaquillas frente économique tels que le rendement de latex. cost efficiency. a las producidas por injerto en lo que se refiere a factores de gran importancia econó- Mots-clés: Hevea brasiliensis, embryo- mica tales como el rendimiento en látex. genèse somatique, bouture, clones, rajeu- nissement, production industrielle , pied- Palabras clave: Hevea brasiliensis, embrio- mère, rentabilité. génesis somática, estaquillas, clones, reju- venecimiento, producción industrial, planta madre, rentabilidad. BOIS ET FORÊTS DES TROPIQUES, 2013, N° 317 (3) PROPAGATION D’HÉVÉA / LE POINT SUR… 53 Introduction Hevea brasiliensis Muëll. Arg. (rubber tree) is a species belonging to the Euphorbiaceae family and originated from the South American Amazonian forest. Being the main source of natural rubber, it has been domesticated and intro- duced in many countries of Africa and South East Asia to be exploited in large estates since the end of the 19th century. This expansion was warranted by an increasing need for tyres and the development of new ways of transportation. Rubber plantations were originally established with unse- lected seedlings resulting in high heterogeneity and very low yield (300 to 600 kg of dry rubber (DR) per ha per year). Since the 1920s, bud-grafted clones have been used in breeding programs and have contributed to a dramatic yield increase (2,500 to 3,500 kg of DR/ha/y). The “Wickham” population exported from Brazil to Asia via Kew Gardens in 1876 has been the first and only gene pool used in H. brasiliensis breeding, until recent genetic enrichment by other sources Photograph 1. (CLEMENT-DEMANGE et al., 2007). Genetic improvement Acclimatization of the in vitro plantlets in SoGB nursery facilities. through conventional breeding is hampered by a long imma- Photograph A. Masson. ture period, an insufficient fruit production and a high level of heterozygosity. Development of superior H. brasiliensis clones remains a long term and high-investment process: RRIM600 which is currently the most
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