DOCSLIB.ORG

Investigation of Gram-Negative Bacterial

Total Page：16

File Type：pdf, Size：1020Kb

Download full-text PDF Read full-text
Investigation of Gram-Negative Bacterial Investigation of Gram-negative bacterial surface glycans: characterisation of Moraxella bovis lipooligosaccharide and progress towards developing a Nontypeable Haemophilus influenzae/Moraxella catarrhalis vaccine candidate Author Singh, Sanjesh Published 2019 Thesis Type Thesis (PhD Doctorate) School School of Medical Science DOI https://doi.org/10.25904/1912/2443 Copyright Statement The author owns the copyright in this thesis, unless stated otherwise. Downloaded from http://hdl.handle.net/10072/388637 Griffith Research Online https://research-repository.griffith.edu.au Investigation of Gram-negative bacterial surface glycans: characterisation of Moraxella bovis lipooligosaccharide and progress towards developing a Nontypeable Haemophilus influenzae/Moraxella catarrhalis vaccine candidate TITLE PAGE Sanjesh Singh BHSc, MMedRes A Thesis Submitted to the Griffith Graduate Research School for the Fulfilment of the Requirement for the Degree of Doctor of Philosophy December 2018 Menzies Health Institute, School of Medical Science & Institute for Glycomics Griffith University “Failure is the condiment that gives success its flavour” Truman Capote ii STATEMENT OF ORIGINALITY This work has not been previously submitted for a degree or diploma in any university. To the best of my knowledge and belief, the thesis contains no material previously published or written by another person except where due reference is made in the thesis itself. Sanjesh Singh December 2018 iii ACKNOWLEDGMENTS This project would not have been possible without the financial support from the School of Medical Science, Griffith University Postgraduate Research Scholarship and The John Viller’s Trust. I would like to extend my sincere thanks to the School of Medical Science and Institute for Glycomics for permitting me to undertake this project and to use their facilities. I am especially grateful and indebted to Associate Professor Jennifer Wilson and Dr Darren Grice, my Co-Principal supervisors, for the opportunity to work in their labs and for supporting me throughout my academic career. I’m thankful for your patience, guidance, providing me with your own areas of expert knowledge and giving me the confidence to work independently on my experiments. I could not have asked for better role models. Additionally, thank you for allowing me to learn and grow under your guidance, especially proof-reading my articles and helping me with my imperfect English. I would also like to thank Dr Ian Peak for his insightful experimental suggestions, practical assistance and reading my drafts. Thank you for your guidance especially in the middle ear vaccine project and helping me interpret data from the animal experiments. I am grateful to all of those with whom I have had the pleasure to work in the chemistry and microbiology laboratories: Rebecca, Milton, Monika, Dylan, Keith, Courtney, Tyler, Evan, Wisam, Deniz, Matt and other co-lab members. I am thankful to the animal house staff, especially Allanah, Carly, Hailey, Karen and Hamish, for all your support and assistance provided for preforming the immunogenicity of vaccines in mice. To my parents, Karan and Jayanti and sibling, Jayeshma, thank you so much for your endless support, patience and always being there when I needed you the most. Your constant love and care has kept me going throughout my PhD. Most importantly, I wish to thank my loving and supportive partner, Victoria, who has been an inspiration, always stood by my side and encouraged me throughout my PhD. You have been my strength. I can’t describe how thankful and grateful I am to have you four in my life. I hope I have made you all proud. iv TABLE OF CONTENTS Contents TITLE PAGE .............................................................................................................................. i STATEMENT OF ORIGINALITY ......................................................................................... iii ACKNOWLEDGMENTS ........................................................................................................ iv TABLE OF CONTENTS ........................................................................................................... v LIST OF FIGURES .................................................................................................................. ix LIST OF TABLES ..................................................................................................................... x ABBREVIATIONS .................................................................................................................. xi ALL PAPERS INCLUDED ARE CO-AUTHORED .............................................................. xv PUBLICATIONS AND RELEVANT COMMUNICATIONS BY THE CANDIDATE ..... xvii ABSTRACT ............................................................................................................................ xix CHAPTER 1 .............................................................................................................................. 1 Introduction and Literature Review ....................................................................................... 2 1.1 Gram-Negative Bacteria ................................................................................................... 2 1.1.1 Gram-Negative Bacterial Mechanisms of Pathogenesis ........................................... 2 1.1.2 Lipopolysaccharide/Lipooligosaccharide .................................................................. 2 1.1.3 Outer Membrane Proteins .......................................................................................... 5 1.1.4 The Innate Immune System ....................................................................................... 6 1.1.4.1 Specific Molecular Interactions Between Lipid A/TLR4/MD2/CD14 Complex elucidated by X-Ray Crystal Structure Analysis ............................................................ 8 1.1.5 The Adaptive Immune System ................................................................................ 10 1.1.6 Antibody Isotypes and Subclasses ........................................................................... 11 1.1.7 Antibody-Mediated Effector Functions ................................................................... 12 1.1.8 Recent concerns with antimicrobial resistance and current glycocongujate vaccine approach to address this concern ...................................................................................... 13 1.2 Moraxella bovis .............................................................................................................. 15 1.2.1 Infectious Bovine Keratoconjunctivitis (IBK) ‘Pinkeye’ ........................................ 15 1.2.2 Clinical Presentation and Progression of Infectious Bovine Keratoconjunctivitis .. 16 1.2.3 Pathogenicity – Cell Surface Associated Virulence Profile .................................... 17 1.2.3.1 Pili ..................................................................................................................... 17 1.2.3.2 Hemolysin ......................................................................................................... 17 1.2.3.3 Lipooligosaccharide .......................................................................................... 18 v 1.2.3.4 Capsule .............................................................................................................. 19 1.2.4 Vaccine Candidates ................................................................................................. 20 1.2.5 Antibiotics and Developing Resistance ................................................................... 21 1.3 Moraxella catarrhalis .................................................................................................... 22 1.3.1 M. catarrhalis: An Important Pathogen .................................................................. 22 1.3.2 Colonisation ............................................................................................................. 22 1.3.3 Acute Otitis Media................................................................................................... 23 1.3.4 Chronic Obstructive Pulmonary Disease ................................................................. 25 1.3.5 Pathogenicity – Cell Surface Associated Virulence Profile .................................... 26 1.3.5.1 Lipooligosaccharide .......................................................................................... 27 1.3.5.2 Outer Membrane Proteins ................................................................................. 31 1.3.6 Vaccine Candidates ................................................................................................. 31 1.3.7 Antibiotic Treatment and Developing Resistance ................................................... 34 1.4 Haemophilus influenzae ................................................................................................. 34 1.4.1 Nontypeable Haemophilus influenzae ..................................................................... 34 1.4.2 Colonisation ............................................................................................................. 35 1.4.3 Acute Otitis Media................................................................................................... 35 1.4.4 Chronic Obstructive Pulmonary Disease ................................................................. 35 1.4.5 Pathogenicity – Cell Surface Associated Virulence Profile ...................................
Load more

Recommended publications
  • Biofilm Formation by Moraxella Catarrhalis
    BIOFILM FORMATION BY MORAXELLA CATARRHALIS APPROVED BY SUPERVISORY COMMITTEE Eric J. Hansen, Ph.D. ___________________________ Kevin S. McIver, Ph.D. ___________________________ Michael V. Norgard, Ph.D. ___________________________ Philip J. Thomas, Ph.D. ___________________________ Nicolai S.C. van Oers, Ph.D. ___________________________ BIOFILM FORMATION BY MORAXELLA CATARRHALIS by MELANIE MICHELLE PEARSON DISSERTATION Presented to the Faculty of the Graduate School of Biomedical Sciences The University of Texas Southwestern Medical Center at Dallas In Partial Fulfillment of the Requirements For the Degree of DOCTOR OF PHILOSOPHY The University of Texas Southwestern Medical Center at Dallas Dallas, Texas March, 2004 Copyright by Melanie Michelle Pearson 2004 All Rights Reserved Acknowledgements As with any grand endeavor, there was a large supporting cast who guided me through the completion of my Ph.D. First and foremost, I would like to thank my mentor, Dr. Eric Hansen, for granting me the independence to pursue my ideas while helping me shape my work into a coherent story. I have seen that the time involved in supervising a graduate student is tremendous, and I am grateful for his advice and support. The members of my graduate committee (Drs. Michael Norgard, Kevin McIver, Phil Thomas, and Nicolai van Oers) have likewise given me a considerable investment of time and intellect. Many of the faculty, postdocs, students and staff of the Microbiology department have added to my education and made my experience here positive. Many members of the Hansen laboratory contributed to my work. Dr. Eric Lafontaine gave me my first introduction to M. catarrhalis. I hope I have learned from his example of patience, good nature, and hard work.
    [Show full text]
  • Blocking Transmission of Vector-Borne Diseases
    International Journal for Parasitology: Drugs and Drug Resistance 7 (2017) 90e109 Contents lists available at ScienceDirect International Journal for Parasitology: Drugs and Drug Resistance journal homepage: www.elsevier.com/locate/ijpddr Review Blocking transmission of vector-borne diseases * Sandra Schorderet-Weber a, Sandra Noack b, Paul M. Selzer b, Ronald Kaminsky c, a Sablons 30, 2000 Neuchatel,^ Switzerland b Boehringer Ingelheim Animal Health GmbH, Binger Str. 173, 55216 Ingelheim, Germany c ParaC Consulting for Parasitology and Drug Discovery, Altenstein 13, 79685 Haeg-Ehrsberg, Germany article info abstract Article history: Vector-borne diseases are responsible for significant health problems in humans, as well as in companion Received 14 November 2016 and farm animals. Killing the vectors with ectoparasitic drugs before they have the opportunity to pass Accepted 22 January 2017 on their pathogens could be the ideal way to prevent vector borne diseases. Blocking of transmission Available online 30 January 2017 might work when transmission is delayed during blood meal, as often happens in ticks. The recently described systemic isoxazolines have been shown to successfully prevent disease transmission under Keywords: conditions of delayed pathogen transfer. However, if the pathogen is transmitted immediately at bite as it Vector-borne diseases is the case with most insects, blocking transmission becomes only possible if ectoparasiticides prevent Transmission blocking Drug discovery the vector from landing on or, at least, from biting the host. Chemical entities exhibiting repellent activity Speed of kill in addition to fast killing, like pyrethroids, could prevent pathogen transmission even in cases of im- mediate transfer. Successful blocking depends on effective action in the context of the extremely diverse life-cycles of vectors and vector-borne pathogens of medical and veterinary importance which are summarized in this review.
    [Show full text]
  • Escherichia Coli Saccharomyces Cerevisiae Bacillus Subtilis はB
    研究開発等に係る遺伝子組換え生物等の第二種使用等に当たって執るべき拡散防止措 置等を定める省令の規定に基づき認定宿主ベクター系等を定める件 （平成十六年一月二十九日文部科学省告示第七号） 最終改正：令和三年二月十五日文部科学省告示第十三号 （認定宿主ベクター系） 第一条 研究開発等に係る遺伝子組換え生物等の第二種使用等に当たって執るべき拡散防止 措置等を定める省令（以下「省令」という。）第二条第十三号の文部科学大臣が定める認 定宿主ベクター系は、別表第一に掲げるとおりとする。 （実験分類の区分ごとの微生物等） 第二条 省令第三条の表第一号から第四号までの文部科学大臣が定める微生物等は、別表第 二の上欄に掲げる区分について、それぞれ同表の下欄に掲げるとおりとする。 （特定認定宿主ベクター系） 第三条 省令第五条第一号ロの文部科学大臣が定める特定認定宿主ベクター系は、別表第一 の２の項に掲げる認定宿主ベクター系とする。 （自立的な増殖力及び感染力を保持したウイルス及びウイロイド） 第四条 省令別表第一第一号ヘの文部科学大臣が定めるウイルス及びウイロイドは、別表第 三に掲げるとおりとする。 別表第１（第１条関係） 区 分 名 称 宿主及びベクターの組合せ １ Ｂ１ (１) ＥＫ１ Escherichia coli K12株、B株、C株及びW株又は これら各株の誘導体を宿主とし、プラスミド又は バクテリオファージの核酸であって、接合等によ り宿主以外の細菌に伝達されないものをベクター とするもの（次項(１)のＥＫ２に該当するものを除 く。） (２) ＳＣ１ Saccharomyces cerevisiae又はこれと交雑可能な 分類学上の種に属する酵母を宿主とし、これらの 宿主のプラスミド、ミニクロモソーム又はこれら の誘導体をベクターとするもの（次項(２)のＳＣ２ に該当するものを除く。） (３) ＢＳ１ Bacillus subtilis Marburg168株、この誘導体又 はB. licheniformis全株のうち、アミノ酸若しく は核酸塩基に対する複数の栄養要求性突然変異を 有する株又は胞子を形成しない株を宿主とし、こ れらの宿主のプラスミド（接合による伝達性のな いものに限る。）又はバクテリオファージの核酸 をベクターとするもの（次項(３)のＢＳ２に該当す るものを除く。） (４) Thermus属細菌 Thermus属細菌（T. thermophilus、T. aquaticus、 T. flavus、T. caldophilus及びT. ruberに限る。） を宿主とし、これらの宿主のプラスミド又はこの 誘導体をベクターとするもの (５) Rhizobium属細菌 Rhizobium属細菌（R. radiobacter（別名Agroba- cterium tumefaciens）及びR. rhizogenes（別名 Agrobacterium rhizogenes）に限る。）を宿主と し、これらの宿主のプラスミド又はRK2系のプラ スミドをベクターとするもの (６) Pseudomonas putida Pseudomonas putida KT2440株又はこの誘導体を 宿主とし、これら宿主への依存性が高く、宿主以 外の細胞に伝達されないものをベクターとするも の (７) Streptomyces属細菌 Streptomyces属細菌（S. avermitilis、S. coel- icolor [S. violaceoruberとして分類されるS. coelicolor A3(２)株を含む]、S. lividans、S. p- arvulus、S. griseus及びS.
    [Show full text]
  • Neisseria Acinetobacter, Moraxella ? and Kingella Based on Partial 16S Ribosomal DNA Sequence Analysis4
    INTERNATIONALJOURNAL OF SYSTEMATICBACTERIOLOGY, July 1994, p. 387-391 Vol. 44, No. 3 0020-7713/94/$04.00 + 0 Copyright 0 1994, International Union of Microbiological Societies Phylogenetic Relationships between Some Members of the Genera Neisseria Acinetobacter, Moraxella ? and Kingella Based on Partial 16s Ribosomal DNA Sequence Analysis4. M. C. ENRIGHT,” P. E. CARTER, I. A. MACLEAN,AND H. McKENZIE Department of Medical Microbiology, Faculty of Medicine, University of Aberdeen, Foresterhill, Aberdeen, Scotland AB9 220 We obtained 16s ribosomal DNA (rDNA) sequence data for strains belonging to 11 species of Proteobacteria, including the type strains of Kingella kingae, Neisseria lactamica, Neisseria meningitidis, Moraxella lacunata subsp. lacunata, [Neisseria] ovis, Moraxella catarrhalis, Moraxella osloensis, [Moraxella] phenylpyruvica, and Acineto- bacter lwo$i, as well as strains of Neisseria subflava and Acinetobacter calcoaceticus. The data in a distance matrix constructed by comparing the sequences supported the proposal that the genera Acinetobacter and Moraxella and [N.] ovis should be excluded from the family Neisseriaceae. Our results are consistent with hybridization data which suggest that these excluded taxa should be part of a new family, the Moraxellaceae. The strains that we studied can be divided into the following five groups: (i) M. lacunata subsp. lacunata, [N.] ovis, and M. catarrhalis; (ii) M. osloensis; (iii) [M.]phenylpyruvica; (iv) A. calcoaceticus and A. lwofii; and (v) N. meningitidis, N. subflava, N. lactamica, and K. kingae. We agree with the previous proposal that [N.] ovis should be renamed Moraxella ovis, as this organism is closely related to Moraxella species and not to Neisseria species. The generically misnamed taxon [M.] phenylpyruvica belongs to the proposed family Moraxellaceae, but it is sufficiently different to warrant exclusion from the genus Moraxella.
    [Show full text]
  • Acinetobacter Baumannii – a Neglected Pathogen in Veterinary and Environmental Health in Germany
    Veterinary Research Communications (2019) 43:1–6 https://doi.org/10.1007/s11259-018-9742-0 REVIEW ARTICLE Acinetobacter baumannii – a neglected pathogen in veterinary and environmental health in Germany Gamal Wareth1 & Heinrich Neubauer1 & Lisa D. Sprague1 Received: 25 October 2018 /Accepted: 6 December 2018 /Published online: 27 December 2018 # The Author(s) 2018 Abstract The emergence and global spread of drug resistant Acinetobacter (A.) baumannii is a cause of great concern. The current knowledge on antibiotic resistance in A. baumannii from animal origin is mostly based on few internationally published case reports, investigations of strain collections and several whole genome analyses. This lack of data results in a somewhat sketchy picture on how to assess the possible impact of drug resistant A. baumannii strains on veterinary and public health in Germany. Consequently, there is an urgent need to intensify the surveillance of A. baumannii in pet animals, the farm animal population and wildlife. Keywords Acinetobacter baumannii . Review . Antibiotic resistance . Germany Introduction limited number of widespread clones appear to be responsible for hospital outbreaks in many countries (Diancourt et al. Acinetobacter (A.) baumannii is a gram-negative opportunis- 2010). Eight international clonal lineages have been described tic nosocomial pathogen belonging to the genus Acinetobacter so far and comparative typing of outbreak strains obtained all and a member of the family Moraxellaceae. Acinetobacter over Europe revealed the dominance of three clones, original- spp. are non-motile, non-fastidious Gram-negative, non- ly named European clones I-III (Dijkshoorn et al. 1996; Karah fermenting, catalase positive, oxidase negative, strictly aero- et al.
    [Show full text]
  • Downloaded Skin Zaka Electronic Gmbh, Cologne, Germany)
    Li et al. Microbiome (2021) 9:47 https://doi.org/10.1186/s40168-020-00995-7 RESEARCH Open Access Characterization of the human skin resistome and identification of two microbiota cutotypes Zhiming Li1,2†, Jingjing Xia3,4,5†, Liuyiqi Jiang3†, Yimei Tan4,6†, Yitai An1,2, Xingyu Zhu4,7, Jie Ruan1,2, Zhihua Chen1,2, Hefu Zhen1,2, Yanyun Ma4,7, Zhuye Jie1,2, Liang Xiao1,2, Huanming Yang1,2, Jian Wang1,2, Karsten Kristiansen1,2,8, Xun Xu1,2,9, Li Jin4,10, Chao Nie1,2*, Jean Krutmann4,5,11*, Xiao Liu1,12,13* and Jiucun Wang3,4,10,14* Abstract Background: The human skin microbiota is considered to be essential for skin homeostasis and barrier function. Comprehensive analyses of its function would substantially benefit from a catalog of reference genes derived from metagenomic sequencing. The existing catalog for the human skin microbiome is based on samples from limited individuals from a single cohort on reference genomes, which limits the coverage of global skin microbiome diversity. Results: In the present study, we have used shotgun metagenomics to newly sequence 822 skin samples from Han Chinese, which were subsequently combined with 538 previously sequenced North American samples to construct an integrated Human Skin Microbial Gene Catalog (iHSMGC). The iHSMGC comprised 10,930,638 genes with the detection of 4,879,024 new genes. Characterization of the human skin resistome based on iHSMGC confirmed that skin commensals, such as Staphylococcus spp, are an important reservoir of antibiotic resistance genes (ARGs). Further analyses of skin microbial ARGs detected microbe-specific and skin site-specific ARG signatures.
    [Show full text]
  • Linking the Resistome and Plasmidome to the Microbiome
    The ISME Journal (2019) 13:2437–2446 https://doi.org/10.1038/s41396-019-0446-4 ARTICLE Linking the resistome and plasmidome to the microbiome 1,2 3 3 3 1,2 Thibault Stalder ● Maximilian O. Press ● Shawn Sullivan ● Ivan Liachko ● Eva M. Top Received: 15 February 2019 / Revised: 2 May 2019 / Accepted: 10 May 2019 / Published online: 30 May 2019 © The Author(s) 2019. This article is published with open access Abstract The rapid spread of antibiotic resistance among bacterial pathogens is a serious human health threat. While a range of environments have been identified as reservoirs of antibiotic resistance genes (ARGs), we lack understanding of the origins of these ARGs and their spread from environment to clinic. This is partly due to our inability to identify the natural bacterial hosts of ARGs and the mobile genetic elements that mediate this spread, such as plasmids and integrons. Here we demonstrate that the in vivo proximity-ligation method Hi-C can reconstruct a known plasmid-host association from a wastewater community, and identify the in situ host range of ARGs, plasmids, and integrons by physically linking them to their host chromosomes. Hi-C detected both previously known and novel associations between ARGs, mobile genetic elements and host genomes, thus validating this method. We showed that IncQ plasmids and class 1 integrons had the broadest host range in this wastewater, and identified bacteria belonging to Moraxellaceae, Bacteroides,andPrevotella, and 1234567890();,: 1234567890();,: especially Aeromonadaceae as the most likely reservoirs of ARGs in this community. A better identification of the natural carriers of ARGs will aid the development of strategies to limit resistance spread to pathogens.
    [Show full text]
  • Phase and Antigenic Variation Mediated by Genome Modifications
    Antonie van Leeuwenhoek (2008) 94:493–515 DOI 10.1007/s10482-008-9267-6 REVIEW PAPER Phase and antigenic variation mediated by genome modifications Florence Wisniewski-Dye´ Æ Ludovic Vial Received: 6 April 2008 / Accepted: 9 July 2008 / Published online: 29 July 2008 Ó Springer Science+Business Media B.V. 2008 Abstract Phase and antigenic variation is used by Keywords Adaptation Á Antigenic variation Á several bacterial species to generate intra-population Genome plasticity Á Phenotypic switch diversity that increases bacterial fitness and is important in niche adaptation, or to escape host defences. By this adaptive process, bacteria undergo frequent and usually reversible phenotypic changes Introduction resulting from genetic or epigenetic alterations at specific genetic loci. Phase variation or phenotypic One of the most obvious features of phase variation is switch allows the expression of a given phenotype to the appearance of a minority of colonies or colony be switched ON or OFF. Antigenic variation refers to sectors displaying a different aspect. Phase variation the expression of a number of alternative forms of an or phenotypic switch is used by several bacterial antigen on the cell surface, and at a molecular level, species to generate intra-population diversity that shares common features with phase variation mech- increases bacterial fitness and is important in niche anisms. This review will focus on phase and antigenic adaptation, or to escape host defences. Phase varia- variation mechanisms implying genome modifica- tion allows that the expression of a given phenotype tions, with an emphasis on the diversity of is either ON or OFF; these events are usually phenotypes regulated by these mechanisms, and the reversible (ON $ OFF) but may be irreversible ecological relevance of variant appearance within a (ON ? OFF or OFF ? ON), and result from genetic given population.
    [Show full text]
  • Cave-73-02-Fullr.Pdf
    EDITORIAL Production Changes for Future Publication of the Journal of Cave and Karst Studies SCOTT ENGEL Production Editor The Journal of Cave and Karst Studies has experienced December 2011 issue, printed copies of the Journal will be budget shortfalls for the last several years for a multitude automatically distributed to paid subscribers, institutions, of reasons that include, but are not limited to, increased and only those NSS members with active Life and cost of paper, increased costs of shipping through the Sustaining level memberships. The remainder of the NSS United State Postal Service, increased submissions, and membership will be able to view the Journal electronically stagnant funding from the National Speleological Society online but will not automatically receive a printed copy. Full (NSS). The cost to produce the Journal has increased 5 to content of each issue of the Journal will be available for 20 percent per year for the last five years, yet the budget for viewing and downloading in PDF format at no cost from the the Journal has remained unchanged. To offset rising costs, Journal website www.caves.org/pub/journal. the Journal has implemented numerous changes over recent Anyone wishing to receive a printed copy of the Journal years to streamline the production and printing process. will be able to subscribe for an additional cost separate However, the increasing production costs, combined with from normal NSS dues. The cost and subscription process the increasing rate of good-quality submissions, has were still being determined at the time of this printing. resulted in the number of accepted manuscripts by the Once determined, the subscription information will be Journal growing faster than the acquisition of funding to posted on the Journal website.
    [Show full text]
  • Characterization of Environmental and Cultivable Antibiotic- Resistant Microbial Communities Associated with Wastewater Treatment
    antibiotics Article Characterization of Environmental and Cultivable Antibiotic- Resistant Microbial Communities Associated with Wastewater Treatment Alicia Sorgen 1, James Johnson 2, Kevin Lambirth 2, Sandra M. Clinton 3 , Molly Redmond 1 , Anthony Fodor 2 and Cynthia Gibas 2,* 1 Department of Biological Sciences, University of North Carolina at Charlotte, Charlotte, NC 28223, USA; [email protected] (A.S.); [email protected] (M.R.) 2 Department of Bioinformatics and Genomics, University of North Carolina at Charlotte, Charlotte, NC 28223, USA; [email protected] (J.J.); [email protected] (K.L.); [email protected] (A.F.) 3 Department of Geography & Earth Sciences, University of North Carolina at Charlotte, Charlotte, NC 28223, USA; [email protected] * Correspondence: [email protected]; Tel.: +1-704-687-8378 Abstract: Bacterial resistance to antibiotics is a growing global concern, threatening human and environmental health, particularly among urban populations. Wastewater treatment plants (WWTPs) are thought to be “hotspots” for antibiotic resistance dissemination. The conditions of WWTPs, in conjunction with the persistence of commonly used antibiotics, may favor the selection and transfer of resistance genes among bacterial populations. WWTPs provide an important ecological niche to examine the spread of antibiotic resistance. We used heterotrophic plate count methods to identify Citation: Sorgen, A.; Johnson, J.; phenotypically resistant cultivable portions of these bacterial communities and characterized the Lambirth, K.; Clinton,
    [Show full text]
  • Moraxella Species: Infectious Microbes Identified by Use of Time-Of
    Japanese Journal of Ophthalmology (2019) 63:328–336 https://doi.org/10.1007/s10384-019-00669-4 CLINICAL INVESTIGATION Moraxella species: infectious microbes identifed by use of time‑of‑fight mass spectrometry Shunsuke Takahashi1 · Kazuhiro Murata1 · Kenji Ozawa1 · Hiroki Yamada2 · Hideaki Kawakami3 · Asami Nakayama4 · Yuko Asano5 · Kiyofumi Mochizuki1 · Hiroshige Mikamo6 Received: 14 August 2018 / Accepted: 2 April 2019 / Published online: 4 July 2019 © Japanese Ophthalmological Society 2019 Abstract Purpose To report the clinical manifestations, identifcation, antimicrobial susceptibilities, and treatment outcomes of ocular infections caused by Moraxella species. Study design Retrospective study. Patients and methods The medical records of all patients treated at the Departments of Ophthalmology of the Ogaki Munici- pal Hospital and the Gifu University Graduate School of Medicine for ocular infections caused by Moraxella species between January 2011 and June 2017 were examined. The stored Moraxella species isolated from ocular samples were identifed by matrix-assisted laser desorption/ionization time-of-fight mass spectrometry (MALDI-TOF MS), molecular identifcation, and the biochemical properties. Results Sixteen eyes of 16 patients were treated for Moraxella ocular infections. The patients’ median age was 72 years. A predisposing systemic or ocular condition was identifed in 15 of the patients. Nine of the patients developed keratitis; four, conjunctivitis; and three, blebitis. M lacunata (6 eyes), M catarrhalis (6), M nonliquefaciens (3), and M osloensis (1) were identifed by MALDI-TOF MS. All isolates were sensitive to levofoxacin, tobramycin, ceftazidime, ceftriaxone, and cefa- zolin. Twelve patients with keratitis or blebitis were treated with various topical antimicrobial combinations, and systemic antibiotics were used in 10 of the 12 patients.
    [Show full text]
  • Table S5. the Information of the Bacteria Annotated in the Soil Community at Species Level
    Table S5. The information of the bacteria annotated in the soil community at species level No. Phylum Class Order Family Genus Species The number of contigs Abundance(%) 1 Firmicutes Bacilli Bacillales Bacillaceae Bacillus Bacillus cereus 1749 5.145782459 2 Bacteroidetes Cytophagia Cytophagales Hymenobacteraceae Hymenobacter Hymenobacter sedentarius 1538 4.52499338 3 Gemmatimonadetes Gemmatimonadetes Gemmatimonadales Gemmatimonadaceae Gemmatirosa Gemmatirosa kalamazoonesis 1020 3.000970902 4 Proteobacteria Alphaproteobacteria Sphingomonadales Sphingomonadaceae Sphingomonas Sphingomonas indica 797 2.344876284 5 Firmicutes Bacilli Lactobacillales Streptococcaceae Lactococcus Lactococcus piscium 542 1.594633558 6 Actinobacteria Thermoleophilia Solirubrobacterales Conexibacteraceae Conexibacter Conexibacter woesei 471 1.385742446 7 Proteobacteria Alphaproteobacteria Sphingomonadales Sphingomonadaceae Sphingomonas Sphingomonas taxi 430 1.265115184 8 Proteobacteria Alphaproteobacteria Sphingomonadales Sphingomonadaceae Sphingomonas Sphingomonas wittichii 388 1.141545794 9 Proteobacteria Alphaproteobacteria Sphingomonadales Sphingomonadaceae Sphingomonas Sphingomonas sp. FARSPH 298 0.876754244 10 Proteobacteria Alphaproteobacteria Sphingomonadales Sphingomonadaceae Sphingomonas Sorangium cellulosum 260 0.764953367 11 Proteobacteria Deltaproteobacteria Myxococcales Polyangiaceae Sorangium Sphingomonas sp. Cra20 260 0.764953367 12 Proteobacteria Alphaproteobacteria Sphingomonadales Sphingomonadaceae Sphingomonas Sphingomonas panacis 252 0.741416341
    [Show full text]