ENVIS CENTER on ENVIRONMENTAL BIOTECHNOLOGY
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Optimization of Agro-Residues As Substrates for Pleurotus
Wu et al. AMB Expr (2019) 9:184 https://doi.org/10.1186/s13568-019-0907-1 ORIGINAL ARTICLE Open Access Optimization of agro-residues as substrates for Pleurotus pulmonarius production Nan Wu1†, Fenghua Tian1†, Odeshnee Moodley1, Bing Song1, Chuanwen Jia1, Jianqiang Ye2, Ruina Lv1, Zhi Qin3 and Changtian Li1* Abstract The “replacing wood by grass” project can partially resolve the confict between mushroom production and balancing the ecosystem, while promoting agricultural economic sustainability. Pleurotus pulmonarius is an economically impor- tant edible and medicinal mushroom, which is traditionally produced using a substrate consisting of sawdust and cottonseed hulls, supplemented with wheat bran. A simplex lattice design was applied to systemically optimize the cultivation of P. pulmonarius using agro-residues as the main substrate to replace sawdust and cottonseed hulls. The efects of difering amounts of wheat straw, corn straw, and soybean straw on the variables of yield, mycelial growth rate, stipe length, pileus length, pileus width, and time to harvest were demonstrated. Results indicated that a mix of wheat straw, corn straw, and soybean straw may have signifcantly positive efects on each of these variables. The high yield comprehensive formula was then optimized to include 40.4% wheat straw, 20.3% corn straw, 18.3% soybean straw, combined with 20.0% wheat bran, and 1.0% light CaCO3 (C/N 42.50). The biological efciency was 15.2% greater than that of the control. Most encouraging was the indication= that the high yield comprehensive formula may shorten the time to reach the reproductive stage by 6 days, compared with the control. -
Oyster Mushroom) Related with Its Chemical Composition: a Review on the Past Decade Findings
Biotechnological, nutritional and therapeutic uses of Pleurotus spp. (Oyster mushroom) related with its chemical composition: A review on the past decade findings Rúbia Carvalho Gomes Corrêaa,b,c, Tatiane Brugnaric, Adelar Brachtc, Rosane Marina Peraltac, Isabel C.F.R. Ferreiraa,* aMountain Research Centre (CIMO), ESA, Polytechnic Institute of Bragança, Campus de Santa Apolónia, 1172, 5301-855 Bragança, Portugal. bCAPES Foundation, Ministry of Education of Brazil, 70.040-020, Brasília, DF, Brazil. cState University of Maringá, Department of Biochemistry, 87020-900, Maringá, PR, Brazil. * Author to whom correspondence should be addressed (Isabel C.F.R. Ferreira; e-mail: [email protected]; telephone +351-273-303219; fax +351-273-325405). 1 Abstract Background: The particular characteristics of growth and development of mushrooms in nature result in the accumulation of a variety of secondary metabolites, several of them with biological activities. The genus Pleurotus is a cosmopolitan group of mushrooms with high nutritional value and therapeutic properties, besides a wide array of biotechnological and environmental applications. Scope and approach: The present report aims to provide a critical review on aspects related to chemical compounds isolated from the genus Pleurotus with possible biotechnological, nutritional and therapeutic uses. Investigations on the genus have immensely accelerated during the last ten years, so that only reports published after 2005 have been considered. Key findings and conclusions: The most important Pleurotus species cultivated in large scale are P. ostreatus and P. pulmonarius. However, more than 200 species have already been investigated to various degrees. Both basidiomata and mycelia of Pleurotus are a great renewable and easily accessible source of functional foods/nutraceuticals and pharmaceuticals with antioxidant, antimicrobial, anti- inflammatory, antitumor and immunomodulatory effects. -
Transcriptional and Enzymatic Profiling of Pleurotus Ostreatus
View metadata, citation and similar papers at core.ac.uk brought to you by CORE provided by Academica-e Transcriptional and Enzymatic Profiling of Pleurotus ostreatus Laccase Genes in Submerged and Solid-State Fermentation Cultures Raúl Castanera,a Gúmer Pérez,a Alejandra Omarini,a Manuel Alfaro,a Antonio G. Pisabarro,a Vincenza Faraco,b,c Antonella Amore,b and Lucía Ramíreza Genetics and Microbiology Research Group, Department of Agrarian Production, Public University of Navarre, Pamplona, Spaina; Department of Organic Chemistry and Biochemistry, Complesso Universitario Monte S. Angelo, University of Naples Federico II, Naples, Italyb; and School of Biotechnological Sciences, University of Naples Federico II, Naples, Italyc The genome of the white rot basidiomycete Pleurotus ostreatus includes 12 phenol oxidase (laccase) genes. In this study, we ex- amined their expression profiles in different fungal strains under different culture conditions (submerged and solid cultures) Downloaded from and in the presence of a wheat straw extract, which was used as an inducer of the laccase gene family. We used a reverse tran- scription-quantitative PCR (RT-qPCR)-based approach and focused on determining the reaction parameters (in particular, the reference gene set for the normalization and reaction efficiency determinations) used to achieve an accurate estimation of the relative gene expression values. The results suggested that (i) laccase gene transcription is upregulated in the induced submerged fermentation (iSmF) cultures but downregulated in the solid fermentation (SSF) cultures, (ii) the Lacc2 and Lacc10 genes are the main sources of laccase activity in the iSmF cultures upon induction with water-soluble wheat straw extracts, and (iii) an addi- tional, as-yet-uncharacterized activity (Unk1) is specifically induced in SSF cultures that complements the activity of Lacc2 and Lacc10. -
Structural Characterization and Biological Activity of Lactarius Scrobiculatus
Structural characterization and biological activity of Lactarius scrobiculatus Ivana Tomic Thesis for the Master´ degree in Pharmacy 45 study points Department of Pharmaceutical Chemistry School of Pharmacy Faculty of Mathematics and Natural Sciences UNIVERSITY OF OSLO November/2018 II Structural characterization and biological activity of Lactarius scrobiculatus Thesis for Master´ degree in Pharmacy Department for Pharmaceutical chemistry School of Pharmacy Faculty of Mathematics and Natural Sciences University in Oslo Ivana Tomic November 2018 Supervisor: Anne Berit Samuelsen III © Author 2018 Structural characterization and biological activity of Lactarius scrobiculatus Ivana Tomic http://www.duo.uio.no/ Print: Reprosentralen, Universitetet i Oslo IV Acknowledgments The present thesis was carried out at the Departement of Pharmaceutical Chemistry, University of Oslo (UiO), for the Master´s degree in Pharmacy at the University of Oslo. The other institute include Norwegian Centre of Molecular Medicine, where I have performed activity assay. First and foremost, I would like to thank to my supervisor Anne Berit Samuelsen for hers support and guidance throughout my work and useful comments during the writing. Further, I also want to thank Hoai Thi Nguyen and Cristian Winther Wold for help with carrying out GC and GC-MS analysis. Also, I am very thankful to Karl Malterud for help with NMR analysis. Special thanks to Suthajini Yogarajah for her patience and lab support. I would also like to thank to Kari Inngjerdingen for good and helpful Forskningforberedende kurs. My gratitude goes also to Prebens Morth group at NMCC, special to Julia Weikum and Bojana Sredic, who were always kind and helpful. Finally, I would like to express my fabulous thanks to my wonderful parents, my husband and my four sons for their great patience, sacrifice, moral support and encouragement during my master thesis. -
Vascular Flora and Vegetation Classification of the South Atlantic Coastal Plain Limestone Forest Association of Central Georgia
VASCULAR FLORA AND VEGETATION CLASSIFICATION OF THE SOUTH ATLANTIC COASTAL PLAIN LIMESTONE FOREST ASSOCIATION OF CENTRAL GEORGIA by PATRICK SUMNER LYNCH (Under the Direction of J.L. Hamrick) ABSTRACT The South Atlantic Coastal Plain Limestone forest is a globally imperiled (G2) forest association known only from the upper Coastal Plain of central Georgia. These calcareous forest communities support diverse floristic assemblages unique among the Georgia Coastal Plain, but have not been subject to detailed floristic study. I conducted a comprehensive floristic inventory, multivariate community analyses and floristic quality assessments to document composition, elucidate community structure and underlying physiographic regimes, and assess habitat integrity for seven sites in Houston, Bleckley and Twiggs counties. Community analyses revealed twelve community types within two floristically defined domains corresponding to uplands and slopes, and bottomlands, respectively, and governed largely by moisture content and degree of inclination. Floristic quality assessments revealed varying degrees of floristic quality and habitat integrity corresponding primarily to local physiography and disturbance history. Floristic inventory recovered 339 vascular plant taxa representing 218 genera in 98 families, including 17 rare Georgia species. INDEX WORDS: Limestone forest, community analysis, floristic inventory, floristic quality assessment, Georgia, Coastal Plain. VASCULAR FLORA AND VEGETATION CLASSIFICATION OF THE SOUTH ATLANTIC COASTAL PLAIN LIMESTONE FOREST ASSOCIATION OF CENTRAL GEORGIA by PATRICK SUMNER LYNCH B.S., The University of Illinois, Urbana-Champaign, 2006 A Thesis Submitted to the Graduate Faculty of The University of Georgia in Partial Fulfillment of the Requirements for the Degree MASTER OF SCIENCE ATHENS, GEORGIA 2010 © 2012 Patrick Sumner Lynch All Rights Reserved VASCULAR FLORA AND VEGETATION CLASSIFICATION OF THE SOUTH ATLANTIC COASTAL PLAIN LIMESTONE FOREST ASSOCIATION OF CENTRAL GEORGIA by PATRICK SUMNER LYNCH Major Professor: J.L. -
Bioactive Compounds and Medicinal Properties of Oyster Mushrooms (Pleurotus Sp.)
FOLIA HORTICULTURAE Folia Hort. 30(2), 2018, 191-201 Published by the Polish Society DOI: 10.2478/fhort-2018-0012 for Horticultural Science since 1989 REVIEW Open access www.foliahort.ogr.ur.krakow.pl Bioactive compounds and medicinal properties of Oyster mushrooms (Pleurotus sp.) Iwona Golak-Siwulska, Alina Kałużewicz*, Tomasz Spiżewski, Marek Siwulski, Krzysztof Sobieralski Department of Vegetable Crops Faculty of Horticulture and Landscape Architecture, Poznań University of Life Sciences Dąbrowskiego 159, Poznań, Poland ABSTRACT There are about 40 species in the Pleurotus genus, including those with high economic significance, i.e. P. ostreatus and P. pulmonarius. The fruiting bodies of oyster mushrooms are of high nutritional and health- promoting value. In addition, many species belonging to the Pleurotus genus have been used as sources of substances with documented medicinal properties, such as high-molecular weight bioactive compounds (polysaccharides, peptides and proteins) and low-molecular weight compounds (terpenoids, fatty acid esters and polyphenols). The bioactive substances contained in the mycelium and fruiting bodies of Pleurotus species exhibit immunostimulatory, anti-neoplastic, anti-diabetic, anti-atherosclerotic, anti-inflammatory, antibacterial and anti-oxidative properties. Their multidirectional positive influence on the human organism is the result of interaction of bioactive substances. Extracts from individual Pleurotus species can be used for the production of dietary supplements increasing the organism’s immunity. -
Morphological and Molecular Identification of Four Brazilian Commercial Isolates of Pleurotus Spp
397 Vol.53, n. 2: pp. 397-408, March-April 2010 BRAZILIAN ARCHIVES OF ISSN 1516-8913 Printed in Brazil BIOLOGY AND TECHNOLOGY AN INTERNATIONAL JOURNAL Morphological and Molecular Identification of four Brazilian Commercial Isolates of Pleurotus spp. and Cultivation on Corncob Nelson Menolli Junior 1,2*,Tatiane Asai 1, Marina Capelari 1 and Luzia Doretto Paccola- 3 Meirelles 1Instituto de Botânica; Núcleo de Pesquisa em Micologia; C. P. 3005; 01061-970; São Paulo - SP - Brasil. 2Instituto Federal de Educação, Ciência e Tecnologia; Rua Pedro Vicente 625; Canindé; 01109-010; São Paulo - SP - Brasil. 3 Universidade Estadual de Londrina; Departamento de Biologia Geral; C. P. 6001; 86051-990; Londrina - PR - Brasil ABSTRACT The species of Pleurotus have great commercial importance and adaptability for growth and fructification within a wide variety of agro-industrial lignocellulosic wastes. In this study, two substrates prepared from ground corncobs supplemented with rice bran and charcoal were tested for mycelium growth kinetics in test tubes and for the cultivation of four Pleurotus commercial isolates in polypropylene bags. The identification of the isolates was based on the morphology of the basidiomata obtained and on sequencing of the LSU rDNA gene. Three isolates were identified as P. ostreatus , and one was identified as P. djamor . All isolates had better in-depth mycelium development in the charcoal-supplemented substrate. In the cultivation experiment, the isolates reacted differently to the two substrates. One isolate showed particularly high growth on the substrate containing charcoal. Key words : charcoal, edible mushroom cultivation, molecular analysis, taxonomy INTRODUCTION sugarcane bagasse, banana skins, corn residues, grass, sawdust, rice and wheat straw, banana The genus Pleurotus (Fr.) P. -
Lilioceris Egena Air Potato Biocontrol Environmental Assessment
United States Department of Field Release of the Beetle Agriculture Lilioceris egena (Coleoptera: Marketing and Regulatory Chrysomelidae) for Classical Programs Biological Control of Air Potato, Dioscorea bulbifera (Dioscoreaceae), in the Continental United States Environmental Assessment, February 2021 Field Release of the Beetle Lilioceris egena (Coleoptera: Chrysomelidae) for Classical Biological Control of Air Potato, Dioscorea bulbifera (Dioscoreaceae), in the Continental United States Environmental Assessment, February 2021 Agency Contact: Colin D. Stewart, Assistant Director Pests, Pathogens, and Biocontrol Permits Plant Protection and Quarantine Animal and Plant Health Inspection Service U.S. Department of Agriculture 4700 River Rd., Unit 133 Riverdale, MD 20737 Non-Discrimination Policy The U.S. Department of Agriculture (USDA) prohibits discrimination against its customers, employees, and applicants for employment on the bases of race, color, national origin, age, disability, sex, gender identity, religion, reprisal, and where applicable, political beliefs, marital status, familial or parental status, sexual orientation, or all or part of an individual's income is derived from any public assistance program, or protected genetic information in employment or in any program or activity conducted or funded by the Department. (Not all prohibited bases will apply to all programs and/or employment activities.) To File an Employment Complaint If you wish to file an employment complaint, you must contact your agency's EEO Counselor (PDF) within 45 days of the date of the alleged discriminatory act, event, or in the case of a personnel action. Additional information can be found online at http://www.ascr.usda.gov/complaint_filing_file.html. To File a Program Complaint If you wish to file a Civil Rights program complaint of discrimination, complete the USDA Program Discrimination Complaint Form (PDF), found online at http://www.ascr.usda.gov/complaint_filing_cust.html, or at any USDA office, or call (866) 632-9992 to request the form. -
Vegetation Community Monitoring at Ocmulgee National Monument, 2011
National Park Service U.S. Department of the Interior Natural Resource Stewardship and Science Vegetation Community Monitoring at Ocmulgee National Monument, 2011 Natural Resource Data Series NPS/SECN/NRDS—2014/702 ON THE COVER Duck potato (Sagittaria latifolia) at Ocmulgee National Monument. Photograph by: Sarah C. Heath, SECN Botanist. Vegetation Community Monitoring at Ocmulgee National Monument, 2011 Natural Resource Data Series NPS/SECN/NRDS—2014/702 Sarah Corbett Heath1 Michael W. Byrne2 1USDI National Park Service Southeast Coast Inventory and Monitoring Network Cumberland Island National Seashore 101 Wheeler Street Saint Marys, Georgia 31558 2USDI National Park Service Southeast Coast Inventory and Monitoring Network 135 Phoenix Road Athens, Georgia 30605 September 2014 U.S. Department of the Interior National Park Service Natural Resource Stewardship and Science Fort Collins, Colorado The National Park Service, Natural Resource Stewardship and Science office in Fort Collins, Colorado, publishes a range of reports that address natural resource topics. These reports are of interest and applicability to a broad audience in the National Park Service and others in natural resource management, including scientists, conservation and environmental constituencies, and the public. The Natural Resource Data Series is intended for the timely release of basic data sets and data summaries. Care has been taken to assure accuracy of raw data values, but a thorough analysis and interpretation of the data has not been completed. Consequently, the initial analyses of data in this report are provisional and subject to change. All manuscripts in the series receive the appropriate level of peer review to ensure that the information is scientifically credible, technically accurate, appropriately written for the intended audience, and designed and published in a professional manner. -
Spatial Distribution of Glucan Type and Content Between Caps and Stalks in Pleurotus Eryngii: Impact on the Anti-Inflammatory Functionality
International Journal of Molecular Sciences Article Spatial Distribution of Glucan Type and Content between Caps and Stalks in Pleurotus eryngii: Impact on the Anti-inflammatory Functionality Vaclav Vetvicka 1, Ofer Gover 2, Hilla Hayby 2, Ofer Danay 3, Nirit Ezov 4, Yitzhak Hadar 5 and Betty Schwartz 2,* 1 University of Louisville, Department of Pathology, University of Louisville, Louisville, KY 40202, USA; [email protected] 2 Institute of Biochemistry, School of Nutritional Sciences, Food Science and Nutrition, The Robert H. Smith Faculty of Agriculture, Food and Environment, The Hebrew University of Jerusalem, Rehovot 76100, Israel; [email protected] (O.G.); [email protected] (H.H.) 3 Edible Mushrooms, MIGAL, 11016 Kiryat Shmona, and Tel Hai College, 12210 Upper Galilee, Israel; [email protected] 4 Edible Mushrooms Development, MIGAL, Kiryat Shmona 11016, Israel; [email protected] 5 Department of Plant Pathology and Microbiology, The Robert H. Smith Faculty of Agriculture, Food and Environment, The Hebrew University of Jerusalem, Rehovot 76100, Israel; [email protected] * Correspondence: [email protected] Received: 8 October 2018; Accepted: 26 October 2018; Published: 28 October 2018 Abstract: Pleurotus eryngii is recognized for its prominent nutritional and medicinal value. In our study, we tested the effect of glucans on lipopolysaccharide (LPS)-induced production of TNF-α. We demonstrated that glucan extracts are more effective than mill mushroom preparations. Additionally, the effectiveness of stalk-derived glucans were slightly more pronounced than of caps. Cap and stalk glucans from mill or isolated glucan competed dose-dependently with anti-Dectin-and anti-CR-3 antibodies, indicating that they contain β-glucans recognized by these receptors. -
Coleoptera: Chrysomelidae) – a Potential Biological Control Agent of Air Potato, Dioscorea Bulbifera (Dioscoreaceae) Robert W
This article was downloaded by: [University of Florida] On: 13 January 2014, At: 06:28 Publisher: Taylor & Francis Informa Ltd Registered in England and Wales Registered Number: 1072954 Registered office: Mortimer House, 37-41 Mortimer Street, London W1T 3JH, UK Biocontrol Science and Technology Publication details, including instructions for authors and subscription information: http://www.tandfonline.com/loi/cbst20 Laboratory host range testing of Lilioceris sp. near impressa (Coleoptera: Chrysomelidae) – a potential biological control agent of air potato, Dioscorea bulbifera (Dioscoreaceae) Robert W. Pemberton a & Gloria L. Witkus a a USDA-ARS Invasive Plant Research Laboratory , Ft. Lauderdale, FL, USA Published online: 01 Mar 2010. To cite this article: Robert W. Pemberton & Gloria L. Witkus (2010) Laboratory host range testing of Lilioceris sp. near impressa (Coleoptera: Chrysomelidae) – a potential biological control agent of air potato, Dioscorea bulbifera (Dioscoreaceae), Biocontrol Science and Technology, 20:6, 567-587, DOI: 10.1080/09583150903531332 To link to this article: http://dx.doi.org/10.1080/09583150903531332 PLEASE SCROLL DOWN FOR ARTICLE Taylor & Francis makes every effort to ensure the accuracy of all the information (the “Content”) contained in the publications on our platform. However, Taylor & Francis, our agents, and our licensors make no representations or warranties whatsoever as to the accuracy, completeness, or suitability for any purpose of the Content. Any opinions and views expressed in this publication are the opinions and views of the authors, and are not the views of or endorsed by Taylor & Francis. The accuracy of the Content should not be relied upon and should be independently verified with primary sources of information. -
Fungal Incidence and Growth of Two Pleurotus
atholog P y & nt a M Sobowale et al., J Plant Pathol Microbiol 2018, 9:8 l i P c f r o o b DOI: 10.4172/2157-7471.1000448 l i Journal of a o l n o r g u y o J ISSN: 2157-7471 Plant Pathology & Microbiology ReviewResearch Article Article OpenOpen Access Access Fungal Incidence and Growth of Two Pleurotus Species on Sawdust of Ceiba pentandra (Linn.) Gaertn and Ficus Mucuso welw (Softwoods) Sobowale AA*, Atoyebi FT and Adenipekun CO Department of Botany, University of Ibadan, Ibadan, Nigeria Abstract Objective: Nigeria is faced with one of the most pressing environmental challenge which is the large production of sawdust waste of which Ceiba pentandra and Ficus mucuso are among the major contributors. This work seeks to harness waste through mushroom cultivation thereby reducing environmental hazards usually caused by improper disposal and burning. Softwood sawdust of Ceiba pentandra and Ficus mucuso was evaluated for their effects on the production of Pleurotus ostreatus and Pleurotus pulmonarius. Similarly, probable relationship among the fungal incidence of the substrates (sawdust) and that of mushrooms were examined. Methods: The treatments used were replicated three times in a completely randomized design. The fruiting bodies were harvested and growth parameters, total yield and biological efficiency (BE) of the mushroom were recorded while their nutritional analysis was carried out. Resident fungi in the sawdust and mushrooms were isolated and identified after obtaining pure cultures. The data obtained were subjected to analysis. Results: Ceiba pentandra and Ficus mucuso sawdust (substrates) supported the growth of the two mushrooms.