DECEMBER 2012

Phaff Collection News

Yeasts of yesterday and today, for research of tomorrow

A big year! The year 2012 was full of activity at the Phaff Culture Collection, including several research projects, and useful additions to the collection catalog through internal research and deposits from external collections and 2012 at the Phaff Yeast Culture researchers. The Phaff collection is also Collection participating in national and The Phaff Yeast Culture Collection is in good company -- there international efforts to improve are a number of excellent yeast culture collections around the the standing of microbial world. To help publicize these collections to potential users in the culture collections. biotechnology field, Boundy-Mills sent a survey to selected yeast The Phaff Yeast Culture collection curators to gather information about uses of their Collection is the fourth largest culture collections. This information was combined with data collection of its kind, with over gleaned from the World Data Centre for Microorganisms website, 7,000 strains in the public and published in the Journal for Industrial Microbiology and catalog. Biotechnology (Boundy-Mills, JIMB 39 (5) 673-680). In this issue Pages 2-3 Page 4 Pages 5-7 Research on olive Interactions with Yeast and spoilage, US and strains available Drosophila/yeast international from the Phaff associations microbe collections collection

PHAFF COLLECTION NEWS DECEMBER 2012

Research publications Phaff collection research related to food spoilage, yeast lipids and yeast/insect ecology

Yeast lipids We are working to develop new yeast oils for fuels, chemicals, and food ingredients. The long-term goals are to identify specific high-oil yeast strains that grow well on specific feedstocks such as agricultural and food processing waste. We recently published an improved screening protocol using Nile Red staining, to identify lipid- accumulating (Sitepu et al., Journal of Microbiological Methods, 91 (2) 321-328). Continuing work on yeast lipids Next up: a survey of dozens of potential lipid- accumulating yeasts. Pectinolytic yeast causes olive

softening In a project led by Maria Marco (Food Science and Technology, UC Davis), we examined microbes associated with spoiled and unspoiled Sicilian-style olives, in an attempt to identify the agent causing softening of the mesocarp. We isolated and identified a number of pectinolytic yeasts, including a particularly active strain of . When this yeast was inoculated into pilot-scale fermentations, the olives became soft, indicating that we may have identified the spoilage culprit. This was published in 2012 (Golomb et al., Food Microbiology 33(1) 97-106). We are now working on olive fermentation inoculation conditions to help prevent this type of spoilage. QUESTION: Could the pectinolytic strains of S. cerevisiae, manshurica, and boidinii that we isolated be useful for conversion of food processing or other plant waste to biofuels?

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PHAFF COLLECTION NEWS DECEMBER 2012

New work on Drosophila-yeast associations

Drosophila melanogaster: In a long series Drosophila suzukii: In a project led by Frank Zalom of studies in collaboration with Judy (Department of Entomology, UC Davis) and his PhD Stamps (Evolution and Ecology, UC student Kelly Hamby and visiting professor Alejandro Davis), we examined the effects of Hernandez (Universidad de Extremadura, Badajoz, Spain), Drosophila melanogaster flies on yeast we studied yeasts associated with the agricultural pest abundance and diversity in banana Drosophila suzukii. This fruit fly recently invaded California, exposed to adults or larvae. We found and infests many soft fruits such as cherries and evidence of niche construction: raspberries. We are working with the Zalom lab to develop presence of flies resulted in more an improved yeast-based lure for this fly. We used culture- predictable numbers of yeasts, and a based and molecular methods to profile yeasts in infested narrower range of species. Larvae cherries and raspberries, and adult flies and larvae, and excrete viable yeasts in their fecal found a variety of yeast species. Surprisingly, just about pools, yeast species that they find every adult fly, larva and infested fruit sampled contained palatable, and discourage growth of Hanseniaspora uvarum. This was published in Applied and filamentous fungi. We published these Environmental Microbiology (Hamby et al., AEM 78(14) observations of proto-agriculture in 4869-4873). A very artistic rendering by Hamby of D. PLoS ONE (Stamps et al. 2012, PLoS suzukii using yeasts on an agar plate was the AEM July ONE 7(7) 1-10). 2012 cover art!

Drosophila as a “model organism” Drawing produced by Kelly Hamby (PhD Student, Entomology, UC Davis)

INGREDIENTS: To highlight our work on yeasts 1 Petri plate associated with the agricultural pest Agar medium Drosophila suzukii (Spotted Wing 2 active cultures of yeasts Drosophila, SWD), graduate student isolated from Drosophila Kelly Hamby produced a drawing of suzukii SWD, using yeasts we isolated from 1 “model organism” willing SWD. Applied and Environmental to pose for a portrait Microbiology used the photo for the Patience cover of their July 2012 issue.

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PHAFF COLLECTION NEWS DECEMBER 2012

Comparing Notes

Networking with collection curators Public microbe collections are an important element of biological research infrastructure. While the collection of viable, pure, properly identified microbes are an essential element of a microbe collection, much more is required: knowledge of , database management, customer service, quality control, intellectual property issues, and adherence to national and international regulations such as IATA and the Convention on Biodiversity. Networking with other collection curators makes these jobs easier. Over the years, the Phaff National networking collection has improved collection management and customer service thanks to advice Kyria Boundy-Mills, curator of the Phaff Yeast Culture from other collection curators. Collection, is on the steering committee of the US Culture Collection Network (USCCN). This five-year, NSF- funded project is led by Kevin McCluskey, curator of the Fungal Genetic Stock Center at the University of Missouri, Kansas City. The goals of the USCCN are to implement a National Microbial Germplasm system, and to foster communication between US collections, foreign collections, and international collection networks. In September 2012, Boundy-Mills attended the kick-off event of the USCCN, a gathering of curators of numerous US culture collections and other stakeholders and policy makers, held in Kansas City, MO in September. Discussions included pricing structures, databasing, quality control, financial support of collections, IP issues and customer service, which are resulting in improved service to Phaff collection users.

International networking

Boundy-Mills was also one of three representatives of the USCCN that attended the first meeting of an EU-funded effort being conducted in Europe called Microbial Resource Research Infrastructure (MIRRI), held in Braunschweig, Germany in December 2012. Implementation of the Convention on Biodiversity and Nagoya Protocol were hot issues. One of the high points of this trip was a tour of the DSMZ collection, an exemplary government-funded microbe collection in Germany.

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PHAFF COLLECTION NEWS DECEMBER 2012

much more Hundreds of yeast species, • OTHER ENVIRONMENTAL: oceans, rivers, lakes, swamps, glaciers, atmosphere, hundreds of potential uses! sewage sludge, wood pulp, soil • CLINICAL ISOLATES: cerebrospinal In 2012, Phaff collection yeasts were distributed fluid, colostrum, dandruff, teeth, feces, hair, to academic, government agency and industrial lung, sputum researchers around the world for a broad variety of uses such as: New additions to the public catalog of the Phaff collection in 2012 include: • Conversion of biomass to biofuels • Control of agricultural pests • Strains of Cryptococcus, Candida and other • Phylogenomics species isolated from oak, birch and • Taxonomy maple, deposited by Joseph Heitman, Duke University • Validation of clinical diagnostic kits • Development of a reference database for • Yeasts isolated from solitary bees and Illumina sequence data analysis their feeding substrates by Ryder Diaz (Evolution and Ecology, UC Davis) • And much more…. • Yeasts isolated from cockroach and stick Does the Phaff collection have what you need insects by Matan Shelomi (Entomology, for your research? The Phaff collection contains UC Davis) 7,000 yeasts belonging to over 750 different • Yeasts and filamentous fungi isolated species, with anywhere from a single strain to from walnut twig (in collaboration over 500 strains per species. While other yeast with Steve Seybold, USDA Forest collections emphasize lab strains, wine strains, Service, Davis, CA) or medical strains, the Phaff collection’s main focus is yeasts isolated from environmental A full strain catalog is available online at habitats. Many yeasts were isolated from www.phaffcollection.org. You can search by decaying plant matter and the insects that genus, species, strain ID number, geographic inhabit them, making them particularly useful location, source habitat, etc. for research on conversion of biomass to value- added products. Examples of habitats of origin include: • FOODS AND BEVERAGES: wine, baking, brewing, spoiled beer, lambic beer, sausage, sauerkraut, olives, traditional fermentations, coffee beans, dairy products, fish, soft drinks, honey, meats • FRUITS AND VEGETABLES: including apple, banana, cherry, raspberry, soybean, many fruit juices • PLANTS: flowers from allium to zinnia, shrubs, grasses, dozens of cactus species, dozens of tree species such as aspen, birch, chestnut, and the rest of the alphabet • INSECTS: ants, , bees, cockroach, Drosophila, lacewing, mites, wasps, and 5 41235

PHAFF COLLECTION NEWS DECEMBER 2012

Yeast species available from the Phaff collection, and the number of strains of each, are listed below. In addition, there are dozens of undescribed species, awaiting names. Contact collection curator Kyria Boundy-Mills for more information, [email protected].

1 Aciculoconidium aculeatum 1 Candida fabianii 3 Candida pini 2 Cryptococcus curvatus 5 Ambrosiozyma angophorae 6 Candida famata 1 Candida ponderosae 5 Cryptococcus dimennae 4 Filobasidium floriforme 1 Ambrosiozyma monospora 1 Candida fermentati 12 Candida populi 2 Cryptococcus ferigula 17 Filobasidium globisporum 1 Ambrosiozyma philentoma 1 Candida flavicans 1 Candida pseudointermedia 1 Cryptococcus festocosus 22 Galactomyces geotrichum 1 Ambrosiozyma platypodis 1 Candida floccose 1 Candida pyralidae 3 Cryptococcus flavescens 5 Galactomyces reessii 83 Aureobasidium pullulans 1 Candida floricola 2 Candida quercitrusa 1 Cryptococcus flavus 2 Geotrichum citri-aurantii 1 Babjaviella inositovora 1 Candida floris 4 Candida quercuum 3 Cryptococcus foliicola 1 Geotrichum fermentans 13 Barnettozyma californica 2 Candida fluviatilis 12 Candida railenensis 3 Cryptococcus gastricus 10 Geotrichum klebahnii 8 Barnettozyma hawaiiensis 2 Candida fragi 3 Candida rancensis 1 Cryptococcus guttulatus 2 Guehomyces pullulans 5 Barnettozyma populi 5 Candida freyschussii 1 Candida rhagii 2 Cryptococcus heveanensis 1 Hanseniaspora clermontiae 1 Barnettozyma pratensis 3 Candida friedrichii 2 Candida rugopelliculosa 35 Cryptococcus humicola 10 Hanseniaspora guilliermondii 3 Barnettozyma salicaria 1 Candida galacta 6 Candida rugosa 1 Cryptococcus infirmo-miniatus 4 Hanseniaspora meyeri 1 Barnettozyma wickerhamii 1 Candida geochares 54 Candida sake 42 Cryptococcus laurentii 5 Hanseniaspora occidentalis 1 Blastobotrys americana 1 Candida gropengiesseri 2 Candida salmanticensis 12 Cryptococcus luteolus 4 Hanseniaspora opuntiae 1 Blastobotrys arbuscula 1 Candida guaymorum 12 Candida santamariae 12 Cryptococcus macerans 5 Hanseniaspora osmophila 1 Blastobotrys capitula 12 Candida guilliermondii 1 Candida santjacobensis 32 Cryptococcus magnus 288 Hanseniaspora uvarum 1 Blastobotrys chiropterorum 1 Candida heliconiae 1 Candida saopaulonensis 2 Cryptococcus marinus 14 Hanseniaspora valbyensis 1 Blastobotrys indianaensis 1 Candida hollandica 1 Candida schatavii 5 Cryptococcus oeirensis 2 Hanseniaspora vineae 1 Blastobotrys muscicola 1 Candida homilentoma 1 Candida scorzettiae 4 Cryptococcus ramirezgomezianus 1 Holtermanniella festucosus 1 Blastobotrys parvus 5 Candida humilis 2 Candida sequanensis 10 Cryptococcus saitoi 5 Hyphopichia burtonii 1 Blastobotrys robertii 1 Candida incommunis 4 Candida shehatae 13 Cryptococcus skinneri 7 Kazachstania exigua 4 Brettanomyces bruxellensis 6 Candida inconspicua 1 Candida silvae 5 Cryptococcus stepposus 1 Kazachstania gamospora 1 1 Candida insectamans 5 Candida silvanorum 1 Cryptococcus tephrensis 4 Kazachstania lodderae 2 Brettanomyces custersianus 8 Candida insectorum 1 Candida silvatica 9 Cryptococcus terreus 1 Kazachstania pintolopesii 2 Brettanomyces naardenensis 5 Candida intermedia 11 Candida silvicola 1 Cryptococcus uniguttulatus 1 Kazachstania rosinii 2 Brettanomyces nanus 1 Candida ipomoeae 15 Candida solani 2 Cryptococcus 1 Kazachstania servazzii 2 Bullera alba 1 Candida ishiwadae 233 Candida sonorensis uzbekistanensis 1 Kazachstania spencerorum 2 Bullera dendrophila 1 Candida jaroonii 2 Candida sorbophila 51 Cryptococcus victoriae 1 Kazachstania telluris 1 Bullera ninhbinhensis 1 Candida kefyr 3 Candida sorbosa 2 Cryptococcus vishniacii 5 Kazachstania unispora 2 Bullera pseudoalba 1 Candida kofuensis 2 Candida sorboxylosa 1 Cryptococcus watticus 1 Kazachstania viticola 2 Bulleromyces albus 1 Candida kruisii 2 Candida spandovensis 3 Cryptococcus wieringae 1 Kloeckera africana 4 Candida aaseri 1 Candida kuoi 1 Candida spencermartinsiae 3 Cuniculitrema polymorpha 10 Kloeckera apiculata 2 Candida abiesophila 3 Candida lactis-condensi 1 Candida sphagnicola 1 Curvibasidium cygneicollum 8 Kloeckera apis 1 Candida alimentaria 8 Candida lambica 1 Candida spherica 3 Cyberlindnera amylophila 1 Kloeckera japonica 1 Candida amapae 1 Candida lassenensis 1 Candida stelleta 1 Cyberlindnera bimundalis 1 Kloeckera javanica 1 Candida ambrosiae 2 Candida leandrae 1 Candida stellimalicola 1 Cyberlindnera euphorbiae 5 Kloeckera lindneri 2 Candida anatomiae 1 Candida lipolytica 1 Candida subhashii 1 Cyberlindnera euphorbiiphila 3 Kluyveromyces aestuarii 2 Candida anglica 1 Candida lipophila 1 Candida succiphila 2 Cyberlindnera fabianii 2 Kluyveromyces dobzhanskii 8 Candida apicola 4 Candida lusitaniae 1 Candida suecica 1 Cyberlindnera lachancei 38 Kluyveromyces lactis 2 Candida apis 1Candida magnoliae 1 Candida suzukii 1 Cyberlindnera lachancei 66 Kluyveromyces marxianus 2 Candida arabinofermentans 7 Candida maltosa 1 Candida tallmaniae 1 Cyberlindnera meyerae 7 Kluyveromyces wickerhamii 1 Candida arcana 1 Candida maris 1 Candida tartarivorans 1 Cyberlindnera misumaiensis 1 Kodamaea laetipori 1 Candida asiatica 16 Candida maritima 9 Candida tenuis 1 Cyberlindnera mrakii 1 Kodamaea nitidulidarum 4 Candida asparagi 2 Candida melibiosica 1 Candida tepae 1 Cyberlindnera rhodanensis 14 Kodamaea ohmeri 1 Candida atlantica 4 Candida membranifaciens 2 Candida torresii 1 Cyberlindnera sargentensis 7 Komagataella pastoris 3 Candida atmosphaerica 8 Candida mesenterica 1 Candida tropicalis 2 Cyberlindnera subsufficiens 1 Komagataella phaffi 1 Candida auringiensis 1 Candida methanosorbosa 1 Candida tsuchiyae 4 Cystofilobasidium bisporidii 1 Komagataella populi 1 Candida azyma 1 Candida milleri 1 Candida ubatubensis 2 Cystofilobasidium capitatum 1 Komagataella pseudopastoris 1 Candida batistae 4 Candida mogii 1 Candida ulmi 30 Cystofilobasidium 1 Kregervanrija delftensis 1 Candida bentonensis 2 Candida molischiana 7 Candida utilis infirmominiatum 15 Kregervanrija fluxuum 84 Candida boidinii 1 Candida montana 15 Candida valida 1 Cystofilobasidium macerans 22 Kuraishia capsulata 1 Candida boleticola 4 Candida multigemmis 2 Candida vartiovaarae 1 Debaryomyces coudertii 1 Kuraishia molischiana 7 Candida bombi 5 Candida musae 2 Candida versatilis 1 Debaryomyces fabryi 1 Kwoniella mangroviensis 1 Candida buinensis 1 Candida mycetangii 14 Candida vini 68 Debaryomyces hansenii 1 Lachancea cidri 50 Candida californica 1 Candida nanaspora 2 Candida wickerhamii 1 Debaryomyces maramus 19 Lachancea fermentati 5 Candida carpophila 3 Candida natalensis 18 Candida wyomingensis 1 Debaryomyces nepalensis 21 Lachancea kluyveri 17 Candida caseinolytica 1 Candida nemodendra 1 Candida xylopsoci 9 Debaryomyces prosopidis 51 Lachancea thermotolerans 1 Candida castellii 5 Candida nitratophila 116 Candida zemplinina 1 Debaryomyces robertsiae 7 Lachancea waltii 6 Candida catenulata 16 Candida norvegica 9 Candida zeylanoides 1 Debaryomyces tamarii 1 Leucosporidiella creatinivora 1 Candida cerambycidarum 1 Candida novakii 1 Cephaloascus fragrans 2 Dekkera anomala 5 Leucosporidiella muscorum 1 Candida chilensis 2 Candida odintsovae 4 Citeromyces hawaiiensis 16 Dekkera bruxellensis 1 Leucosporidium scottii 1 Candida chiropterorum 15 Candida oleophila 7 Citeromyces matritensis 1 Dioszegia catarinonii 3 Lindnera bimundalis 1 Candida cidri 12 Candida orba 1 Cladosporium cladosporioides 1 Dioszegia cryoxerica 1 Lindnera fabianii 2 Candida cleridarum 13 Candida oregonensis 9 Clavispora lusitaniae 1 Dioszegia fristingensis 15 Lindnera lachancei 1 Candida coipomoensis 1 Candida orthopsilosis 138 Clavispora opuntiae 1 Dioszegia hungarica 1 Lindnera mississippiensis 2 Candida cylindracea 1 Candida oslonensis 2 Cryptococcus taibaiensis 1 Dioszegia zsoltii 2 Lindnera misumaiensis 1 Candida dendrica 1 Candida palmioleophila 2 Cryptococcus aerius 2 Dipodascopsis uninucleata 3 Lindnera mrakii 1 Candida dendronema 2 Candida parapsilosis 40 Cryptococcus albidus 16 Dipodascus aggregatus 4 Lindnera rhodanensis 47 Candida deserticola 2 Candida pararugosa 1 Cryptococcus amylolyticus 1 Dipodascus albidus 1 Lindnera sargentensis 11 Candida diddensiae 5 Candida pelliculosa 1 Cryptococcus armeniacus 1 Dipodascus armillariae 9 Lindnera saturnus 4 Candida diversa 1 Candida peltata 1 Cryptococcus aureus 1 Farysizyma acheniorum 1 Lindnera suaveolens 2 Candida elateridarum 2 Candida peoriensis 7 Cryptococcus bhutanensis 1 Farysizyma setubalensis 2 Lindnera veronae 1 Candida emberorum 1 Candida picachoensis 1 Cryptococcus bromeliarum 1 Fellomyces penicillatus 1 Lipomyces arxii 3 Candida eremophila 5 Candida piceae 22 Cryptococcus carnescens 1 Fellomyces polyborus 1 Lipomyces kockii 1 Candida ergatensis 1 Candida picinguabensis 2 Cryptococcus chernovii 1 Fibulobasidium inconspicuum 2 Lipomyces kononenkoae 4 Candida etchellsii 3 Candida pignaliae 1 Cryptococcus cistialbidi 3 Filobasidium capsuligenum 3 Lipomyces lipofer 7 Candida ethanolica 18 Candida pimensis 6 106789

7 Lipomyces starkeyi 1 kodamae 3 Rhodosporidium paludigenum henricii 1 Trichomonascus petasosporus 1 Lipomyces suomiensis 1 Ogataea methanolica 4 Rhodosporidium sphaerocarpum 2 Schizosaccharomyces japonicus 5 Trichosporon asahii 5 Lipomyces tetrasporus 1 Ogataea methylivora 13 Rhodosporidium toruloides 2 Schizosaccharomyces octosporus 1 Trichosporon asteroides 4 Lodderomyces elongisporus 7 Ogataea minuta 9 Rhodotorula araucariae 9 Schizosaccharomyces pombe 2 Trichosporon coremiiforme 8 Magnusiomyces capitatus 2 Ogataea naganishii 7 Rhodotorula aurantiaca 2 Schwanniomyces capriotti 1 Trichosporon cutaneum 5 Magnusiomyces ingens 3 Ogataea philodendri 1 Rhodotorula babjevae 2 Schwanniomyces etchellsii 2 Trichosporon dermatis 6 Magnusiomyces magnusii 2 Ogataea pilisensis 3 Rhodotorula bacarum 23 Schwanniomyces occidentalis 1 Trichosporon domesticum 6 Magnusiomyces ovetensis 15 Ogataea pini 1 Rhodotorula bogoriensis 13 Schwanniomyces polymorphus 1 Trichosporon guehoae 3 Magnusiomyces spicifer 2 1 Rhodotorula buffonii 2 Schwanniomyces 1 Trichosporon insectorum 32 Magnusiomyces starmeri 1 Ogataea populialbae 1 Rhodotorula calyptogenae pseudopolymorphus 2 Trichosporon laibachii 2 Magnusiomyces tetrasperma 1 Ogataea ramenticola 4 Rhodotorula colostri 9 Schwanniomyces vanrijiae 1 Trichosporon montevideense 2 Metschnikowia agaves 1 Ogataea trehaloabstinens 6 Rhodotorula creatinivora 4 Sporidiobolus johnsonii 5 Trichosporon porosum 6 Metschnikowia australis 4 Ogataea trehalophila 3 Rhodotorula dairenensis 2 Sporidiobolus metaroseus 1 Trigonopsis californica 37 Metschnikowia bicuspidata 3 Ogataea wickerhamii 25 Rhodotorula fujisanensis 14 Sporidiobolus pararoseus 3 Trigonopsis variabilis 5 Metschnikowia chrysoperlae 1 Oosporidium margaritiferum 33 Rhodotorula glutinis 11 Sporidiobolus ruineniae 1 Trigonopsis vinaria 1 Metschnikowia corniflorae 3 Pachysolen tannophilus 20 Rhodotorula graminis 23 Sporidiobolus salmonicolor 1 Udeniomyces pannonicus 5 Metschnikowia fructicola 8 Peterozyma toletana 2 Rhodotorula hylophila 2 Sporobolomyces gracilis 1 Udeniomyces puniceus 13 Metschnikowia gruessii 2 Peterozyma xylosa 1 Rhodotorula ingeniosa 3 Sporobolomyces odoratus 3 Udeniomyces pyricola 2 Metschnikowia hawaiiensis 11 Phaffia rhodozyma 3 Rhodotorula lactosa 1 Sporobolomyces oryzicola 1 Ustilago cynodontis 1 Metschnikowia kamienski 20 Phaffomyces antillensis 1 Rhodotorula laryngis 41 Sporobolomyces roseus 3 Vanderwaltozyma polyspora 6 Metschnikowia krissii 42 Phaffomyces opuntiae 4 Rhodotorula marina 1 Sporobolomyces ruberrimus 1 Vanderwaltozyma yarrowii 2 Metschnikowia lunata 27 Phaffomyces thermotolerans 27 Rhodotorula minuta 1 Sporobolomyces singularis 1 Wickerhamia fluorescens 1 Metschnikowia orientalis 33 Pichia barkeri 57 Rhodotorula mucilaginosa 2 Sporobolomyces tsugae 3 Wickerhamomyces alni 1 Metschnikowia proteae 443 Pichia cactophila 1 Rhodotorula nothofagi 128 Sporopachydermia cereana 55 Wickerhamomyces anomalus 39 Metschnikowia pulcherrima 15 Pichia cephalocereana 1 Rhodotorula pallida 2 Sporopachydermia lactativora 1 Wickerhamomyces bisporus 33 Metschnikowia reukaufii 19 Pichia deserticola 3 Rhodotorula pinicola 1 Sporopachydermia oaxacaensis 1 Wickerhamomyces bovis 1 Metschnikowia vanudenii 2 Pichia dianae 1 Rhodotorula pustula 110 Sporopachydermia opuntiana 16 Wickerhamomyces canadensis 8 Metschnikowia zobellii 55 Pichia eremophila 7 Rhodotorula slooffiae 1 Sporopachydermia quercuum 1 Wickerhamomyces chambardii 4 Meyerozyma caribbica 4 Pichia exigua 36 Saccharomyces bayanus 1 Sporopachydermia stenocereana 6 Wickerhamomyces ciferrii 14 Meyerozyma guilliermondii 9 Pichia fermentans 1 Saccharomyces cariocanus 29 Starmera amethionina 1 Wickerhamomyces hampshirensis 1 Millerozyma acaciae 63 Pichia heedii 516 Saccharomyces cerevisiae 38 Starmera caribaea 1 Wickerhamomyces mucosus 7 Millerozyma farinosa 9 Pichia insulana 2 Saccharomyces kudriavzevii 2 Starmera dryadoides 1 Wickerhamomyces onychis 1 Moniliella megachiliens 204 Pichia kluyveri 1 Saccharomyces mikatae 16 Starmera pachycereana 3 Wickerhamomyces pijperi 1 Moniliella suaveolens 62 Pichia kudriavzevii 19 Saccharomyces paradoxus 1 Starmera quercuum 2 Wickerhamomyces silvicola 1 Myxozyma geophila 67 Pichia manshurica 7 Saccharomyces pastorianus 3 Starmerella bombicola 1 Wickerhamomyces strasburgensis 1 Myxozyma kluyveri 69 Pichia membranifaciens 4 Saccharomyces servazzii 1 Sugiyamaella americana 5 Wickerhamomyces strasburgensis 2 Myxozyma melibiosi 9 Pichia nakasei 2 Saccharomyces uvarum 1 Sugiyamaella chiloensis 1 Wickerhamomyces sydowiorum 1 Myxozyma monticola 2 Pichia norvegensis 7 Saccharomycodes ludwigii 1 Taphrina communis 1 Xanthophyllomyces dendrorhous 52 Myxozyma mucilagina 5 Pichia occidentalis 2 Saccharomycodes sinensis 1 Taphrina deformans 36 Yamadazyma mexicana 2 Myxozyma neglecta 23 Pichia pseudocactophila 3 Saccharomycopsis capsularis 1 Taphrina wiesneri 1 Yamadazyma nakazawae 1 Myxozyma neotropica 14 Pichia scutulata 1 Saccharomycopsis crataegensis 1 Tetrapisispora blattae 47 Yamadazyma scolyti 1 Myxozyma nipponensis 48 Pichia terricola 3 Saccharomycopsis fibuligera 1 Tetrapisispora phaffii 3 Yamadazyma triangularis 1 Myxozyma sirexii 11 Priceomyces carsonii 7 Saccharomycopsis javanensis 1 Tilletiopsis washingtonensis 22 Yarrowia lipolytica 1 Myxozyma vanderwaltii 1 Priceomyces castillae 1 Saccharomycopsis malanga 60 Torulaspora delbrueckii 4 Zygoascus hellenicus 2 Nadsonia commutata 3 Priceomyces haplophilus 1 Saccharomycopsis schoenii 4 Torulaspora globosa 3 Zygoascus meyerae 11 Nadsonia fulvescens 1 Priceomyces medius 1 Saccharomycopsis selenospora 3 Torulaspora microellipsoides 1 Zygoascus ofunaensis 2 Nakaseomyces bacillisporus 1 Priceomyces melissophilus 1 Saccharomycopsis synnaedendra 8 Torulaspora pretoriensis 1 Zygoascus tannicolus 3 Nakaseomyces delphensis 1 Pseudozyma aphidis 9 Sakaguchia dacryoidea 2 aurantia 44 Zygosaccharomyces bailii 20 Nakazawaea holstii 1 Pseudozyma graminicola 6 Saprochaete clavata 3 Tremella encephala 8 Zygosaccharomyces bisporus 2 Naumovomyces dairenensis 1 Pseudozyma hubeiensis 2 Saprochaete suaveolens 2 Tremella foliacea 1 Zygosaccharomyces kombuchaensis 1 Naumovozyma castellii 1 Pseudozyma rugulosa 1 Saturnispora besseyi 1 Tremella fuciformis 5 Zygosaccharomyces mellis 1 Occultifur externus 2 Pseudozyma shanxiensis 4 Saturnispora dispora 1 Tremella globispora 18 Zygosaccharomyces rouxii 1 Ogataea allantospora 1 Rhodosporidium paludigenum 4 Saturnispora saitoi 1 Tremella indecorata 2 Zygotorulaspora florentina 1 Ogataea dorogensis 17 Rhodosporidium diobovatum 3 Scheffersomyces spartinae 1 1 Zygotorulaspora mrakii 4 Ogataea glucozyma 1 Rhodosporidium fluviale 17 Scheffersomyces stipitis 2 Tremella moriformis 2 Ogataea henricii 3 Rhodosporidium kratochvilovae 1 Schizoblastosporion starkeyi- 1 Trichomonascus farinosus

Phaff Collection Quick Facts: Contact Information: • Over 7,000 strains in the public Kyria Boundy-Mills, Ph.D., curator catalog Food Science and Technology • Over 750 different yeast species University of California Davis (roughly half of the known yeast One Shields Ave species) Davis, CA 95616 USA • Oldest yeast: isolated by the UC Phone 530-754-5575 Berkeley cellarmaster in 1893 Email [email protected] • Percent of strains not available from Website www.phaffcollection.org any other collection: 80%