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Supplemental Material Supplemental Table 1. Genes activated by alcohol in cultured cortical neurons, as assessed by micro-array analysis. Gene Description Genbank Acc No Folds of increase Gpnmb glycoprotein (transmembrane) nmb NM_053110 2.58 Lyzs lysozyme NM_017372 2.36 Gpnmb glycoprotein (transmembrane) nmb NM_053110 2.33 Gpnmb glycoprotein (transmembrane) nmb NM_053110 2.27 Gpm6a glycoprotein m6a NM_153581 2.05 Mtap1b microtubule-associated protein 1 B NM_008634 2.00 Gfap glial fibrillary acidic protein NM_010277 1.94 C1qg complement component 1, q subcomponent, C chain NM_007574 1.90 C1qb complement component 1, q subcomponent, beta polypeptide, mRNA NM_009777 1.87 Laptm5 lysosomal-associated protein transmembrane 5 NM_010686 1.82 Apoc1 apolipoprotein C-I NM_007469 1.81 Lgals3 lectin, galactose binding, soluble 3 NM_010705 1.81 Fcer1g Fc receptor, IgE, high affinity I, gamma polypeptide NM_010185 1.81 Cd68 CD68 antigen NM_009853 1.81 Apoe apolipoprotein E NM_009696 1.76 C1qa complement component 1, q subcomponent, alpha polypeptide NM_007572 1.75 Lgmn legumain NM_011175 1.74 Msr2 macrophage scavenger receptor 2 NM_030707 1.72 Trem2 triggering receptor expressed on myeloid cells 2 NM_031254 1.72 Serpina3n serine (or cysteine) peptidase inhibitor, clade A, member 3N NM_009252 1.71 Igf1 insulin-like growth factor 1, transcript variant 1 NM_010512 1.71 Ctsz cathepsin Z NM_022325 1.71 Adfp adipose differentiation related protein NM_007408 1.69 Pdgfra platelet derived growth factor receptor, alpha polypeptide NM_011058 1.67 Mmp12 matrix metallopeptidase 12 NM_008605 1.66 Nrgn neurogranin NM_022029 1.65 4831417L10 ELMO domain containing 1 NM_177769 1.64 Cyba cytochrome b-245, alpha polypeptide NM_007806 1.63 Eef2 eukaryotic translation elongation factor 2 NM_007907 1.63 Ctsb cathepsin B NM_007798 1.62 Bcl2a1a B-cell leukemia/lymphoma 2 related protein A1b NM_007534 1.61 Apoc1 apolipoprotein C-I NM_007469 1.61 Abcg1 ATP-binding cassette, sub-family G (WHITE), member 1 NM_009593 1.61 Igf1 insulin-like growth factor 1, transcript variant 2 NM_184052 1.59 Trf transferrin NM_133977 1.57 elf1; elf3 spectrin beta 2, transcript variant 1 NM_175836 1.57 Hnrpk heterogeneous nuclear ribonucleoprotein K NM_025279 1.57 Mtap1b microtubule-associated protein 1 B NM_008634 1.57 Gpc5 glypican 5 NM_175500 1.57 Grn granulin NM_008175 1.57 Fcgr3 Fc receptor, IgG, low affinity III NM_010188 1.56 Bcl2a1d B-cell leukemia/lymphoma 2 related protein A1d NM_007536 1.55 Lpl lipoprotein lipase NM_008509 1.54 1 Sec23a SEC23A (S. cerevisiae) NM_009147 1.53 Ly86 lymphocyte antigen 86 NM_010745 1.53 Syt1 synaptotagmin I NM_009306 1.53 Cdh13 cadherin 13 NM_019707 1.52 Blnk B-cell linker NM_008528 1.52 Sgk serum/glucocorticoid regulated kinase NM_011361 1.51 Igf1 insulin-like growth factor 1, transcript variant 1 NM_010512 1.51 2 Supplemental Table 2. Genes activated by heat shock in cultured cortical neurons, as assessed by micro-array analysis. Gene Description Genbank Acc No Ranking Gucy1a3 guanylate cyclase 1, soluble, alpha 3 (Gucy1a3) NM_021896 3.34 Sv2b synaptic vesicle glycoprotein 2 b (Sv2b), NM_153579 3.10 Dfy Duffy blood group, chemokine receptor (Darc) NM_010045 2.58 Fst follistatin (Fst) NM_008046 2.58 Gucy1a3 guanylate cyclase 1, soluble, alpha 3 (Gucy1a3) NM_021896 2.57 Gpc5 glypican 5 (Gpc5), NM_175500 2.39 Gucy1b3 guanylate cyclase 1, soluble, beta 3 (Gucy1b3) NM_017469 2.38 Nrgn neurogranin (Nrgn) NM_022029 2.38 Tes testis derived transcript (Tes) NM_011570 2.37 Gucy1a3 guanylate cyclase 1, soluble, alpha 3 (Gucy1a3) NM_021896 2.36 3110047P20Rik RIKEN cDNA 3110047P20 gene (3110047P20Rik XM_132047 2.33 Gpr88 G-protein coupled receptor 88 (Gpr88) NM_022427 2.31 Nef3 neurofilament, medium polypeptide (Nefm), NM_008691 2.26 Cbln1 cerebellin 1 precursor protein (Cbln1 NM_019626 2.26 Ttr transthyretin (Ttr), NM_013697 2.24 Efcbp1 EF hand calcium binding protein 1 (Efcbp1), NM_178617 2.23 Pcsk2 proprotein convertase subtilisin/kexin type 2 (Pcsk2), NM_008792 2.22 Spnb3 spectrin beta 3 (Spnb3 XM_129130 2.18 Cdh13 cadherin 13 (Cdh13), NM_019707 2.18 Syt1 synaptotagmin I (Syt1) NM_009306 2.15 Cdh13 cadherin 13 (Cdh13), NM_019707 2.15 Nnat neuronatin (Nnat) NM_010923 2.15 4831417L10 domain containing 1 (Elmod1) NM_177769 2.10 Stmn4 stathmin-like 4 (Stmn4) NM_019675 2.10 Dmn desmuslin (Dmn) NM_207663 2.09 Caln1 calneuron 1 (Caln1) NM_021371 2.09 Prss12 protease, serine, 12 neurotrypsin (motopsin) (Prss12) NM_008939 2.09 Ngef neuronal guanine nucleotide exchange factor (Ngef) NM_019867 2.08 Spock1 sparc/osteonectin, cwcv and kazal-like domains proteoglycan 1 (Spock1) NM_009262 2.06 Hpca hippocalcin (Hpca) NM_010471 2.05 Rac3 RAS-related C3 botulinum substrate 3 (Rac3) NM_133223 2.05 DNA segment, Chr 3, Brigham & Women's Genetics 0562 expressed D3Bwg0562e (D3Bwg0562e) NM_177664 2.05 Ntf3 neurotrophin 3 (Ntf3) NM_008742 2.03 Ppp2r2c protein phosphatase 2, regulatory subunit B (PR 52), gamma isoform (Ppp2r2c) NM_172994 2.01 Syt4 synaptotagmin IV (Syt4) NM_009308 2.00 Epha8 Eph receptor A8 (Epha8) NM_007939 2.00 Zfp537 teashirt zinc finger family member 3 (Tshz3) NM_172298 1.99 Chst8 carbohydrate (N-acetylgalactosamine 4-0) sulfotransferase 8 (Chst8) NM_175140 1.99 Zfp238 zinc finger protein 238 (Zfp238) NM_013915 1.99 AI836003 expressed sequence AI836003 (AI836003) NM_177716 1.98 Slc29a4 solute carrier family 29 (nucleoside transporters), member 4 (Slc29a4) NM_146257 1.98 5330439J01Rik cDNA 5330439J01 gene (5330439J01Rik) NM_175407 1.98 Tes testis derived transcript (Tes) NM_011570 1.98 3 D6Ertd365e zinc and ring finger 2 (Znrf2) NM_199143 1.97 Akt3 thymoma viral proto-oncogene 3 (Akt3) NM_011785 1.97 Tubb4 tubulin, beta 4 (Tubb4) NM_009451 1.97 1700001L19Rik cDNA 1700001L19 gene (1700001L19Rik) XM_127414 1.96 Arrdc4 arrestin domain containing 4 (Arrdc4) NM_025549 1.96 Dmn desmuslin (Dmn) NM_207663 1.94 Ldb2 LIM domain binding 2 (Ldb2) NM_010698 1.94 Smpd3 sphingomyelin phosphodiesterase 3, neutral NM_021491 1.94 Flrt3 fibronectin leucine rich transmembrane protein 3 NM_178382 1.94 Vsnl1 visinin-like 1 NM_012038 1.93 Ldb2 LIM domain binding 2 (Ldb2), transcript variant 1 NM_010698 1.93 Acat2 acetyl-Coenzyme A acetyltransferase 2 NM_009338 1.93 Wsb2 WD repeat and SOCS box-containing 2 NM_021539 1.93 Rab15 RAB15, member RAS oncogene family NM_134050 1.90 Brunol4 bruno-like 4, RNA binding protein (Drosophila) NM_133195 1.89 Emid2 EMI domain containing 2 NM_024474 1.89 elf1; elf3 spectrin beta 2 (Spnb2), transcript variant 1 NM_175836 1.89 3110006E14Rik cDNA 3110006E14 gene (3110006E14Rik) XM_127466 1.88 Lrrn3 leucine rich repeat protein 3, neuronal (Lrrn3) NM_010733 1.88 Slc2a6 solute carrier family 2 (facilitated glucose transporter), member 6 NM_172659 1.88 BC017634 CaM kinase-like vesicle-associated NM_145621 1.87 Lor loricrin NM_008508 1.87 Enc1 ectodermal-neural cortex 1 NM_007930 1.86 Crmp1 collapsin response mediator protein 1 NM_007765 1.86 Cobl cordon-bleu NM_172496 1.86 Sncb synuclein, beta NM_033610 1.86 Neurl neuralized-like homolog (Drosophila) NM_021360 1.85 Bach acyl-CoA thioesterase 7 NM_133348 1.84 Mtap1b microtubule-associated protein 1 B NM_008634 1.84 Nudt4 nudix (nucleoside diphosphate linked moiety X)-type motif 4 NM_027722 1.84 Aacs acetoacetyl-CoA synthetase NM_030210 1.84 Atp9a ATPase, class II, type 9A NM_015731 1.84 Stx1a syntaxin 1A ( NM_016801 1.83 Neo1 neogenin (Neo1), transcript variant 1 NM_008684 1.83 Slco3a1 solute carrier organic anion transporter family, member 3a1 NM_023908 1.83 Ncald neurocalcin delta NM_134094 1.83 Nsdhl NAD(P) dependent steroid dehydrogenase-like NM_010941 1.83 Arhgdig Rho GDP dissociation inhibitor (GDI) gamma NM_008113 1.83 Sqle squalene epoxidase NM_009270 1.83 Eef1a2 eukaryotic translation elongation factor 1 alpha 2 NM_007906 1.82 Syn1 synapsin I NM_013680 1.82 Prkcz protein kinase C, zeta (Prkcz), transcript variant 1 NM_008860 1.82 AI593442 expressed sequence AI593442 (AI593442), transcript variant 1 NM_177907 1.82 4930420O11Rik Mus musculus microtubule associated serine/threonine kinase family XM_283179 1.82 Slc17a6 solute carrier family 17 (sodium-dependent inorganic phosphate cotransporter) NM_080853 1.82 Nsdhl NAD(P) dependent steroid dehydrogenase-like NM_010941 1.81 Lrfn2 leucine rich repeat and fibronectin type III domain containing 2 NM_027452 1.81 4 Atp9a ATPase, class II, type 9A NM_015731 1.81 Fdps farnesyl diphosphate synthetase NM_134469 1.81 Scn3b sodium channel, voltage-gated, type III, beta (Scn3b), transcript variant 2 NM_178227 1.81 Satb2 special AT-rich sequence binding protein 2 NM_139146 1.81 Cdkl2 cyclin-dependent kinase-like 2 (CDC2-related kinase) NM_177270 1.81 Mef2c myocyte enhancer factor 2C NM_025282 1.81 Slc6a17 solute carrier family 6 (neurotransmitter transporter), member 17 NM_172271 1.81 Gprin1 G protein-regulated inducer of neurite outgrowth 1 NM_012014 1.80 Wdr47 WD repeat domain 47 NM_181400 1.80 Met met proto-oncogene NM_008591 1.80 Jakmip1 janus kinase and microtubule interacting protein 1 NM_178394 1.80 Chst1 carbohydrate (keratan sulfate Gal-6) sulfotransferase 1 NM_023850 1.80 Acat2 acetyl-Coenzyme A acetyltransferase 2 NM_009338 1.79 D15Bwg0669e metallophosphoesterase domain containing 1 NM_172610 1.79 Nsdhl NAD(P) dependent steroid dehydrogenase-like NM_010941 1.79 Ly6h lymphocyte antigen 6 complex, locus H NM_011837 1.78 Igsf4b cell adhesion molecule 3 NM_053199 1.78 Sertad4 SERTA domain containing 4 NM_198247 1.78 Hmgcr 3-hydroxy-3-methylglutaryl-Coenzyme A reductase NM_008255 1.78 Gnao1 guanine nucleotide binding protein, alpha o NM_010308 1.77 Pou3f1 POU domain, class
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