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Development of Rapid Gene Expression Analysis and Its ESPOO 2007 VTT PUBLICATIONS 661 VTT PUBLICATIONS 661 Development of rapid gene expression analysis and its application to bioprocess monitoring VTT PUBLICATIONS 661 Development 12345678901234567890123456789012123456789012345678901234567890121234567890123456789012345678901 12345678901234567890123456789012123456789012345678901234567890121234567890123456789012345678901 Bioprocesses are used for production of industrial compounds and for 12345678901234567890123456789012123456789012345678901234567890121234567890123456789012345678901 12345678901234567890123456789012123456789012345678901234567890121234567890123456789012345678901 12345678901234567890123456789012123456789012345678901234567890121234567890123456789012345678901 12345678901234567890123456789012123456789012345678901234567890121234567890123456789012345678901 studying biological phenomena. In order to be able to better understand 12345678901234567890123456789012123456789012345678901234567890121234567890123456789012345678901 12345678901234567890123456789012123456789012345678901234567890121234567890123456789012345678901 12345678901234567890123456789012123456789012345678901234567890121234567890123456789012345678901 and control biological systems, development of methods that generate 12345678901234567890123456789012123456789012345678901234567890121234567890123456789012345678901 12345678901234567890123456789012123456789012345678901234567890121234567890123456789012345678901 12345678901234567890123456789012123456789012345678901234567890121234567890123456789012345678901 12345678901234567890123456789012123456789012345678901234567890121234567890123456789012345678901 detailed information about the physiological status of the living cells is 12345678901234567890123456789012123456789012345678901234567890121234567890123456789012345678901 12345678901234567890123456789012123456789012345678901234567890121234567890123456789012345678901 12345678901234567890123456789012123456789012345678901234567890121234567890123456789012345678901 12345678901234567890123456789012123456789012345678901234567890121234567890123456789012345678901 required. Genetic expression is an important aspect of physiology and 12345678901234567890123456789012123456789012345678901234567890121234567890123456789012345678901 12345678901234567890123456789012123456789012345678901234567890121234567890123456789012345678901 12345678901234567890123456789012123456789012345678901234567890121234567890123456789012345678901 there is an increasing need for technologies enabling time- and cost- 12345678901234567890123456789012123456789012345678901234567890121234567890123456789012345678901 12345678901234567890123456789012123456789012345678901234567890121234567890123456789012345678901 12345678901234567890123456789012123456789012345678901234567890121234567890123456789012345678901 12345678901234567890123456789012123456789012345678901234567890121234567890123456789012345678901 effective analysis of gene transcripts. 12345678901234567890123456789012123456789012345678901234567890121234567890123456789012345678901 12345678901234567890123456789012123456789012345678901234567890121234567890123456789012345678901 12345678901234567890123456789012123456789012345678901234567890121234567890123456789012345678901 The aim of the present study was to develop methods suitable for 12345678901234567890123456789012123456789012345678901234567890121234567890123456789012345678901 12345678901234567890123456789012123456789012345678901234567890121234567890123456789012345678901 12345678901234567890123456789012123456789012345678901234567890121234567890123456789012345678901 12345678901234567890123456789012123456789012345678901234567890121234567890123456789012345678901 transcript analysis of defined gene sets in bioprocess conditions. A 12345678901234567890123456789012123456789012345678901234567890121234567890123456789012345678901 12345678901234567890123456789012123456789012345678901234567890121234567890123456789012345678901 12345678901234567890123456789012123456789012345678901234567890121234567890123456789012345678901 sandwich hybridization assay and a multiplex high-throughput gene 12345678901234567890123456789012123456789012345678901234567890121234567890123456789012345678901 12345678901234567890123456789012123456789012345678901234567890121234567890123456789012345678901 12345678901234567890123456789012123456789012345678901234567890121234567890123456789012345678901 12345678901234567890123456789012123456789012345678901234567890121234567890123456789012345678901 expression analysis called TRAC (Transcript analysis with the aid of 12345678901234567890123456789012123456789012345678901234567890121234567890123456789012345678901 12345678901234567890123456789012123456789012345678901234567890121234567890123456789012345678901 12345678901234567890123456789012123456789012345678901234567890121234567890123456789012345678901 affinity capture) were developed. These methods enable simple and rapid 12345678901234567890123456789012123456789012345678901234567890121234567890123456789012345678901 12345678901234567890123456789012123456789012345678901234567890121234567890123456789012345678901 12345678901234567890123456789012123456789012345678901234567890121234567890123456789012345678901 12345678901234567890123456789012123456789012345678901234567890121234567890123456789012345678901 analysis directly from cell lysates and semi-automated processing of large 12345678901234567890123456789012123456789012345678901234567890121234567890123456789012345678901 12345678901234567890123456789012123456789012345678901234567890121234567890123456789012345678901 12345678901234567890123456789012123456789012345678901234567890121234567890123456789012345678901 sample numbers. The TRAC method was applied for frequent monitoring 12345678901234567890123456789012123456789012345678901234567890121234567890123456789012345678901 12345678901234567890123456789012123456789012345678901234567890121234567890123456789012345678901 12345678901234567890123456789012123456789012345678901234567890121234567890123456789012345678901 12345678901234567890123456789012123456789012345678901234567890121234567890123456789012345678901 of fungal bioprocesses and was shown to have value in physiological 12345678901234567890123456789012123456789012345678901234567890121234567890123456789012345678901 12345678901234567890123456789012123456789012345678901234567890121234567890123456789012345678901 12345678901234567890123456789012123456789012345678901234567890121234567890123456789012345678901 evaluation of the organisms and in process optimization. 12345678901234567890123456789012123456789012345678901234567890121234567890123456789012345678901 12345678901234567890123456789012123456789012345678901234567890121234567890123456789012345678901 12345678901234567890123456789012123456789012345678901234567890121234567890123456789012345678901 12345678901234567890123456789012123456789012345678901234567890121234567890123456789012345678901 12345678901234567890123456789012123456789012345678901234567890121234567890123456789012345678901 12345678901234567890123456789012123456789012345678901234567890121234567890123456789012345678901 12345678901234567890123456789012123456789012345678901234567890121234567890123456789012345678901 12345678901234567890123456789012123456789012345678901234567890121234567890123456789012345678901 12345678901234567890123456789012123456789012345678901234567890121234567890123456789012345678901 12345678901234567890123456789012123456789012345678901234567890121234567890123456789012345678901 12345678901234567890123456789012123456789012345678901234567890121234567890123456789012345678901 12345678901234567890123456789012123456789012345678901234567890121234567890123456789012345678901 12345678901234567890123456789012123456789012345678901234567890121234567890123456789012345678901 12345678901234567890123456789012123456789012345678901234567890121234567890123456789012345678901 12345678901234567890123456789012123456789012345678901234567890121234567890123456789012345678901 12345678901234567890123456789012123456789012345678901234567890121234567890123456789012345678901 12345678901234567890123456789012123456789012345678901234567890121234567890123456789012345678901 12345678901234567890123456789012123456789012345678901234567890121234567890123456789012345678901 12345678901234567890123456789012123456789012345678901234567890121234567890123456789012345678901 12345678901234567890123456789012123456789012345678901234567890121234567890123456789012345678901 12345678901234567890123456789012123456789012345678901234567890121234567890123456789012345678901 12345678901234567890123456789012123456789012345678901234567890121234567890123456789012345678901 12345678901234567890123456789012123456789012345678901234567890121234567890123456789012345678901 12345678901234567890123456789012123456789012345678901234567890121234567890123456789012345678901 12345678901234567890123456789012123456789012345678901234567890121234567890123456789012345678901 12345678901234567890123456789012123456789012345678901234567890121234567890123456789012345678901 12345678901234567890123456789012123456789012345678901234567890121234567890123456789012345678901 Jari Rautio Development of rapid gene expression analysis and its application to bioprocess monitoring Julkaisu on saatavana Publikationen distribueras av This publication is available from VTT VTT VTT PL 1000 PB 1000 P.O. Box 1000 02044 VTT 02044 VTT FI-02044 VTT, Finland Puh. 020 722 4520 Tel. 020 722 4520 Phone internat. + 358 20 722 4520 http://www.vtt.fi http://www.vtt.fi http://www.vtt.fi ISBN 978-951-38-7057-7 (soft back ed.) ISBN 978-951-38-7058-4 (URL: http://www.vtt.fi/publications/index.jsp)
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