US 2015 0080239A1 (19) United States (12) Patent Application Publication (10) Pub. No.: US 2015/0080239 A1 RHODES et al. (43) Pub. Date: Mar. 19, 2015

(54) CLASSIFICATION AND ACTIONABILITY Publication Classification INDICES FOR CANCER (51) Int. Cl. (71) Applicant: LIFE TECHNOLOGIES CI2O I/68 (2006.01) CORPORATION, Carlsbad, CA (US) (52) U.S. Cl. CPC ...... CI2O I/6886 (2013.01); C12O 2600/1 18 (72) Inventors: Daniel RHODES, Ann Arbor, MI (US); (2013.01); C12O 2600/158 (2013.01) Seth SADIS, Ann Arbor, MI (US) USPC ...... SO6/9 (73) Assignee: LIFE TECHNOLOGIES CORPORATION, Carlsbad, CA (US) (57) ABSTRACT (21) Appl. No.: 14/212,717 The disclosure provides compositions, kits, and methods for 1-1. detecting a plurality of genes and associated variants in a (22) Filed: Mar 14, 2014 sample from a subject with cancer. The compositions, kits, O O and methods include a set of oligonucleotides, typically prim Related U.S. Application Data ers and/or probes that can hybridize to identify a gene variant. (60) Provisional application No. 61/877,827, filed on Sep. The methods disclosed herein provide for a mutation status of 13, 2013, provisional application No. 61/891,224, a tumor to be determined and Subsequently associated with a filed on Oct. 15, 2013. report comprising an actionable treatment recommendation. Patent Application Publication Mar. 19, 2015 Sheet 1 of 7 US 2015/0080239 A1

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