Open Access Journal of Veterinary Science & Research ISSN: 2474-9222 Isolation and Identification of Methicilin Resistant Staphylcoccus aureus (MRSA) from Bovine Mastitic Milk in and around Wolaita Sodo, Southern Ethiopia Biniam Tadesse Derib1*, Biruk Tesfaye Birhanu2, Tesfaye Sisay Research Article 3 1 Tessema and Ashenafi Kiros Wubshet Volume 2 Issue 3 1National Animal Health Diagnostic and Investigation Center, Sebeta, Ethiopia Received Date: February 07, 2017 Published Date: June 13, 2017 2Addis Ababa University, College of Veterinary Medicine and Agriculture, Bishoftu, Ethiopia 3Addis Ababa University, institute of biotechnology, Addis Ababa, Ethiopia *Corresponding author: Biniam Tadesse, National Animal Health Diagnostic and Investigation Center, Sebeta, Ethiopia, Email:
[email protected] Abstract In recent years, there has been increased concern about antibiotic resistant strains of Staphylococcus aureus and multiple antibiotic resistant strains have started to emerge. Development of resistance has been attributed to the extensive therapeutic use of antimicrobials. A cross sectional study was conducted from November 2013 to May 2014 in and around Wolaita Sodo town, Southern Ethiopia, to isolate and identify MRSA and their resistance to different antimicrobials and also identify risk factors associated with occurrence of dairy cow mastitis. A total of 257 dairy cows were included during the study period. Total of 1010 quarters were examined to detect clinical and subclinical mastitis by physical examinations of udder and milk and California mastitis Test (CMT), respectively. Milk samples were collected from each of clinically and sub clinically mastitic non-blind quarters of the selected cows for bacterial isolation. The Staphylococcus aureus isolates were tested for anti-microbial susceptibility by disc diffusion method.