Orientation of Loci Within the Human Major Histocompatibility Complex by Chromosomal in Situ Hybridization (Human Leukocyte Antigen/Gene Mapping) CYNTHIA C
Proc. Nati. Acad. Sci. USA Vol. 81, pp. 2816-2820, May 1984 Genetics Orientation of loci within the human major histocompatibility complex by chromosomal in situ hybridization (human leukocyte antigen/gene mapping) CYNTHIA C. MORTON*t, ILAN R. KIRSCHt, WALTER E. NANCE*, GLEN A. EVANS§, ALAN J. KORMAN¶, AND JACK L. STROMINGER¶ *Department of Human Genetics, Medical College of Virginia, Virginia Commonwealth University, Richmond, VA 23298; tNational Cancer Institute, Navy Medical Oncology Branch, The National Naval Medical Center, Building 1, Room 415, Bethesda, MD 20814; §The Cancer Biology Laboratory, The Salk Institute, San Diego, CA 92138; and ¶Fairchild Biochemistry Building, Harvard University, 7 Divinity Avenue, Cambridge, MA 02138 Contributed by Jack L. Strominger, December 29, 1983 ABSTRACT We have determined the localization and ori- with closely linked markers such as glyoxylase 1 (GLO 1) entation of two genetic probes within the human major histo- have permitted inferences to be drawn about the order and compatibility complex by chromosomal in situ hybridization. map distances between marker loci on the proximal portion Our data indicate that a cloned genomic probe cross-hybridiz- of the short arm of chromosome 6, as follows: GLO 1-4 ing to HLA-A, -B, and -C heavy chain loci is homologous to centimorgans (cM)-HLA-DR-0.3 cM-BF, C4B, C4A, sequences located on chromosome 6 at band p21.3 while a sub- C2-0.7 cM-HLA-B-0.1 cM-HLA-C-0.7 cM-HLA-A clone of the genomic HLA-DR a-chain gene corresponding to (16-18). the nonpolymorphic p34 protein is homologous to sequences in Improvements in chromosomal in situ hybridization have band 6p21.1.
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