Commemorating the 1913 Michaelismenten
REVIEW ARTICLE Commemorating the 1913 Michaelis–Menten paper Die Kinetik der Invertinwirkung: three perspectives Ute Deichmann1, Stefan Schuster2, Jean-Pierre Mazat3,4 and Athel Cornish-Bowden5 1 Jacques Loeb Centre for the History and Philosophy of the Life Sciences, Ben-Gurion University of the Negev, Beer Sheva, Israel 2 Department of Bioinformatics, Friedrich Schiller University of Jena, Germany 3 CNRS-UMR 5095, Bordeaux, France 4 University of Bordeaux, France 5 Unite de Bioenerg etique et Ingenierie des Proteines, Institut de Microbiologie de la Mediterran ee, Centre National de la Recherche Scientifique and Aix-Marseille Universite, France Keywords Methods and equations for analysing the kinetics of enzyme-catalysed reac- enzyme kinetics; Henri; history and tions were developed at the beginning of the 20th century in two centres in philosophy; initial rate; Menten; Michaelis; particular; in Paris, by Victor Henri, and, in Berlin, by Leonor Michaelis steady state and Maud Menten. Henri made a detailed analysis of the work in this area Correspondence that had preceded him, and arrived at a correct equation for the initial rate A. Cornish-Bowden, Unitede of reaction. However, his approach was open to the important objection Bioenerg etique et Ingenierie des Proteines, that he took no account of the hydrogen-ion concentration (a subject lar- Institut de Microbiologie, de la gely undeveloped in his time). In addition, although he wrote down an Mediterran ee, Centre National de la expression for the initial rate of reaction and described the hyperbolic form Recherche Scientifique, Aix-Marseille of its dependence on the substrate concentration, he did not appreciate the Universite, 31 chemin Joseph-Aiguier, great advantages that would come from analysis in terms of initial rates 13402 Marseille Cedex 20, France Fax: +33 491 16 40 97 rather than time courses.
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