Action Biology. Advanced Placement for the Second Year. First Edition
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Light-Powered CO 2 Fixation in a Chloroplast Mimic with Natural And
Light-powered CO 2 fixation in a chloroplast mimic with natural and synthetic parts Tarryn Miller, Thomas Beneyton, Thomas Schwander, Christoph Diehl, Mathias Girault, Richard Mclean, Tanguy Chotel, Peter Claus, Niña Socorro Cortina, Jean-Christophe Baret, et al. To cite this version: Tarryn Miller, Thomas Beneyton, Thomas Schwander, Christoph Diehl, Mathias Girault, et al.. Light- powered CO 2 fixation in a chloroplast mimic with natural and synthetic parts. Science, American Association for the Advancement of Science, 2020, 368 (6491), pp.649-654. 10.1126/science.aaz6802. hal-02870971 HAL Id: hal-02870971 https://hal.archives-ouvertes.fr/hal-02870971 Submitted on 24 Feb 2021 HAL is a multi-disciplinary open access L’archive ouverte pluridisciplinaire HAL, est archive for the deposit and dissemination of sci- destinée au dépôt et à la diffusion de documents entific research documents, whether they are pub- scientifiques de niveau recherche, publiés ou non, lished or not. The documents may come from émanant des établissements d’enseignement et de teaching and research institutions in France or recherche français ou étrangers, des laboratoires abroad, or from public or private research centers. publics ou privés. Light-powered CO2 fixation in a chloroplast mimic with natural and synthetic parts Tarryn E. Miller1, Thomas Beneyton3, Thomas Schwander1, Christoph Diehl1, Mathias Girault3, Richard McLean1, Tanguy Chotel3, Peter Claus1, Niña Socorro Cortina1, Jean- Christophe Baret3,4*, Tobias J. Erb1,2* 1Max Planck Institute for terrestrial Microbiology, Department of Biochemistry and Synthetic Metabolism 2Center for Synthetic Microbiology, Karl-von-Frisch-Str. 10, D-35043 Marburg, Germany. 3Univ. Bordeaux, CNRS, CRPP UMR 5031, Pessac, France. -
Self-Assembly of the Protocell from a Self-Ordered Polymer"
https://ntrs.nasa.gov/search.jsp?R=19680013724 2020-03-12T08:56:08+00:00Z Self-Assembly of the Protocell from a Self-ordered Polymer" Paper presented to International Convention of Biochemists, Bangalore, India 7 September 1967 GPO PR,CE $ % CFSTI PRICE(S) $ Hard copy (HC)- - Microfiche (MF) - - ff 653 July65 I *Since 1960, this research has been aided by the National Aeronautics and Space Administration, currently Grant no. NsG-689. Contribution no. 096 of the Institute of Molecular Evolution. Sidney W. Fox Institute of Molecular Evolution University of Miami Coral Gables, Florida, U.S.A. -1- The problem of the origin of life, or in truly perceptive nineteenth century terms, the problem of spontaneous generation, has often been regarded as one of overwhelming complexity. Upon analysis, with the aid of hindsight, this problem loses some of its imponderability. The aspect of evolution which first received major attention was that of the progression, in principle, from primitive cell to contemporary cell and to contemporary multicellular organisms, This stage is the one that has been illuminated mechanistically by Darwin's theory of selection. We can now regard this stage as far more intri- cate and involved than the emergence of primitive life from the primordial reactant gases. By such an analysis, the primordial cell is emphasized, the highly ramified later stages are removed from purview, and the limits of the meaning- ful problem are identified. The preorganismic stage can also be analyzed. For intellectual convenience, it may be divided into two or three parts. The first of these parts is that of the spontaneous organic synthesis involved in the production of the small organic molecules which are necessary for contemporary and, presumably for, primitive organisms. -
Cell Paintballing Using Optically Targeted Coacervate Microdroplets† Cite This: Chem
Chemical Science View Article Online EDGE ARTICLE View Journal | View Issue Cell paintballing using optically targeted coacervate microdroplets† Cite this: Chem. Sci.,2015,6,6106 James P. K. Armstrong,‡*abc Sam N. Olof,‡§*abd Monika D. Jakimowicz,abcd Anthony P. Hollander,{c Stephen Mann,b Sean A. Davis,b Mervyn J. Miles,d Avinash J. Patil*b and Adam W. Perriman*bc We present a new approach for the directed delivery of biomolecular payloads to individual cells with high spatial precision. This was accomplished via active sequestration of proteins, oligonucleotides or molecular dyes into coacervate microdroplets, which were then delivered to specific regions of stem cell membranes using a dynamic holographic assembler, resulting in spontaneous coacervate microdroplet–membrane Received 23rd June 2015 fusion. The facile preparation, high sequestration efficiency and inherent membrane affinity of the Accepted 20th July 2015 microdroplets make this novel “cell paintballing” technology a highly advantageous option for spatially- DOI: 10.1039/c5sc02266e directed cell functionalization, with potential applications in single cell stimulation, transfection and www.rsc.org/chemicalscience differentiation. Creative Commons Attribution 3.0 Unported Licence. Introduction approaches have been used to visualize sub-cellular structure,7 provide nutrients during tissue engineering,8 improve in vivo The emergence of crosscutting techniques such as cellular cell targeting,9 facilitate the external manipulation of cells,10 levitation,1 bio-microuidics2 and live-cell microscopy,3 as well and initiate signalling cascades for cancer therapies.11 Such as advances in single-cell proteomics,4 genomics5 and spec- biotechnologies are limited to some extent by their indiscrim- troscopy,6 has created a demand for new approaches to inter- inate nature, with widespread membrane labelling of the entire rogate cells on an individual basis. -
Marsilea Vestita Hooker & Grev., HAIRY PEPPERWORT. Perennial
Marsilea vestita Hooker & Grev., HAIRY PEPPERWORT. Perennial herb (aquatic or terrestrial), clonal, rhizomatous, fibrous-rooted at nodes, rosetted with acaulous plantlets along horizontal rhizomes, aquatic form with floating leaves (not observed), land form with ascending leaves to 16 cm tall; shoot rosettes with 1−several leaves at each node along rhizome, blades exhibiting sleep movements, very young leaves arising from folded and indistinctly coiled fiddleheads, shoots in range soft-hairy but especially densely stiff- hairy at nodes; rhizomes creeping, congested (mother plant) but often with aboveground, slender, unbranched, stolonlike extensions, cylindric, to 1 mm diameter, internodes of stolonlike rhizome to 20 mm long and sparsely hairy. Leaves (fronds): helically alternate, pinnately compound and 4-foliolate (appearing palmately compound) of 2 opposite pairs of leaflets and having a very short rachis, long-petiolate; petiole (stipe) of land leaves cylindric, (13−)25−150+ × to 0.7 mm, tough, ± villous, hairy at expanded top; rachis to 0.5 mm long; petiolules to 1 mm long, pulvinuslike; blades of leaflets fan-shaped, in range (3−)5−17 × (2−)4−13 mm, thin, triangular-tapered at base, entire, rounded at tip and often reddish along edge, finely ± dichotomously veined with some cross veins, villous with mostly appressed hairs. Sporocarp (sporangium case): containing elongate sori of male and female sporangia, attached on stiff stalk at base of each moderate-sized leaf just above mud level; stalk ascending, unbranched, ± 5 mm long + ridge -
RNA Localization (1960-1961)
Tuesday. Tissue-Specific Gene Expression III (1957-1959) 337a 1957 1958 ANTIGEN PRESENTATION AFTER DNA VACCINATION: GENERATION DIFFERENTIAL DISPLAY RT-PCR (DDRT-PCR) AS A TOOL TO STUDY OF IMMUNE RESPONSES BY EXPRESSION OF A VIRAL PROTEIN IN GENE EXPRESSION: OPTIMIZATION AND APPLICATION. ((K.R. MUSCLE CELLS IN VIVO. ((effrey B. Ulmer, R. Randall Deck, Corrille M. Luehrsenl, M.G. Brubacherl, S. Cumberledge2, A. Lloyd3, and P.E. DeWitt, John J. Donnelly, and Margaret A. Liu)) Department of Virus & Cell MayrandI)) IPerkin Elmer Corp., Applied Biosystems Division, Foster City, Biology, Merck Reearch Laboratories, West Point, PA 19486. CA 94404; 2Dept. of Biochemistry, Univ. of Massachusetts, Amherst, MA CA 94305. Transfection of muscle cells viw has been acdhieved by intramuscular 01003; 3Dept. of Biological Sciences, Stanford Univ., Stanford, injection of naked plasmid DNA (Wolff ct al, Science 247, 1465, 1990). We gene underlies the phenotypic differences between cell utilized this technique as a novel means of vaccination and generated protective Differential expression types. is a standard tool to identify cell-mediated and humoral immune responses agaist influenza in mice (Uner cDNA library construction and screening and clone the sequences of differentially expressed mRNAs. However, c al, Science 259, 1745, 1993). Tlhse results sugested that muscle cells may be in antigen presentation leading to the generation of the immune traditional cDNA libraries that rely on plasmid or phage vectors and passage involved E. from several drawbacks including the requirement of responses observed after DNA injection. However, injected DNA may have been through coli suffer libraries intralized and expessed by other cells and, moreover, muscle cells are not large amounts of polyA+ mRNA, an intensive labor commitment and considered to be antigen presenting cells. -
Phase Transition of RNA−Protein Complexes Into Ordered Hollow Condensates
Phase transition of RNA−protein complexes into ordered hollow condensates Ibraheem Alshareedaha,1, Mahdi Muhammad Moosaa,1, Muralikrishna Rajub, Davit A. Potoyanb,2, and Priya R. Banerjeea,2 aDepartment of Physics, University at Buffalo, The State University of New York, Buffalo, NY 14260; and bDepartment of Chemistry, Iowa State University, Ames, IA 50011 Edited by David A. Weitz, Harvard University, Cambridge, MA, and approved May 27, 2020 (received for review December 20, 2019) Liquid−liquid phase separation of multivalent intrinsically disor- morphologies (14–16).Inanothersystem,itwasobservedthat dered protein−RNA complexes is ubiquitous in both natural and simple overexpression of TDP-43, a stress granule protein, biomimetic systems. So far, isotropic liquid droplets are the most can give rise to multilayered compartments with vacuolated commonly observed topology of RNA−protein condensates in ex- nucleoplasm-filled internal space (17). However, the physical periments and simulations. Here, by systematically studying the driving forces behind these hollow morphologies remain phase behavior of RNA−protein complexes across varied mixture poorly understood. compositions, we report a hollow vesicle-like condensate phase of Recently, we demonstrated that RNA can mediate a reentrant nucleoprotein assemblies that is distinct from RNA−protein drop- phase transition of ribonucleoproteins (RNPs) containing arginine- lets. We show that these vesicular condensates are stable at specific rich low-complexity domains (LCDs) through multivalent heterotypic mixture compositions and concentration regimes within the phase interactions (18, 19). At the substoichiometric regime, RNA triggers diagram and are formed through the phase separation of anisotropic RNP phase separation, whereas, at superstoichiometric ratios, excess protein−RNA complexes. Similar to membranes composed of am- RNA leads to droplet dissolution due to charge inversion on the phiphilic lipids, these nucleoprotein−RNA vesicular membranes ex- surface of RNP−RNA complexes (18). -
(B.Sc.) in Biological Sciences Under CBCS Department of Life Science
Structure and Detailed Syllabus of the Undergraduate Course (B.Sc.) in Biological Sciences under CBCS Department of Life Sciences Presidency University Department of Life Sciences (Faculty of Natural and Mathematical Sciences) Presidency University Hindoo College (1817-1855), Presidency College (1855-2010) 86/1, College Street, Kolkata - 700 073 West Bengal, India 0 | P a g e Content Topic Page No. A. Semester-wise Course Structure and Module Compositions 3 B. Detailed Syllabus for respective Modules 6 Core Course BIOS01C1: Chemistry 5 BIOS01C2: Light and Life 6 BIOS02C3: Biophysics 7 BIOS02C4: Biodiversity 8 BIOS03C5: Proteins and Enzymes 10 BIOS03C6: Cell Biology 11 BIOS03C7: Ecology 13 BIOS04C8: Systems Physiology 14 BIOS04C9: Molecular Biology 15 BIOS04C10: Metabolism and Integration 17 BIOS05C11: Growth and Reproduction 18 BIOS05C12: Genetics 19 BIOS06C13: Defense Mechanisms 21 BIOS06C14: Evolutionary Biology 22 Discipline Specific Elective BIOS05DSE1: Biostatistics & Bioinformatics 25 BIOS05DSE2: Analytical Techniques in Biology 26 BIOS06DSE3: Stress Biology 27 BIOS06DSE4: Classification, Biosystematics and Molecular Analysis 28 Ability Enhancement Compulsory Course AE-1: English communication AE-2: Environmental science Skill Enhancement Elective Courses BIOS03SEC1: Public Health and Management 29 1 | P a g e BIOS04SEC2: Recombinant DNA Technology 29 Generic Elective (GE) BIOS01GE1: World of Animals 31 BIOS02GE2: Economic applications of plant and microbial biotechnology 31 BIOS03GE3: Modern Lifestyle, Behaviors and Ailments 32 -
Multiphase Complex Coacervate Droplets Tiemei Lu and Evan Spruijt*
This is an open access article published under a Creative Commons Non-Commercial No Derivative Works (CC-BY-NC-ND) Attribution License, which permits copying and redistribution of the article, and creation of adaptations, all for non-commercial purposes. pubs.acs.org/JACS Article Multiphase Complex Coacervate Droplets Tiemei Lu and Evan Spruijt* Cite This: https://dx.doi.org/10.1021/jacs.9b11468 Read Online ACCESS Metrics & More Article Recommendations *sı Supporting Information ABSTRACT: Liquid−liquid phase separation plays an important role in cellular organization. Many subcellular condensed bodies are hierarchically organized into multiple coexisting domains or layers. However, our molecular understanding of the assembly and internal organization of these multicomponent droplets is still incomplete, and rules for the coexistence of condensed phases are lacking. Here, we show that the formation of hierarchically organized multiphase droplets with up to three coexisting layers is a generic phenomenon in mixtures of complex coacervates, which serve as models of charge- driven liquid−liquid phase separated systems. We present simple theoretical guidelines to explain both the hierarchical arrangement and the demixing transition in multiphase droplets using the interfacial tensions and critical salt concentration as inputs. Multiple coacervates can coexist if they differ sufficiently in macromolecular density, and we show that the associated differences in critical salt concentration can be used to predict multiphase droplet formation. We also show that the coexisting coacervates present distinct chemical environments that can concentrate guest molecules to different extents. Our findings suggest that condensate immiscibility may be a very general feature in biological systems, which could be exploited to design self-organized synthetic compartments to control biomolecular processes. -
Energy, Structure and Carbon Dioxide: a Realistic View of the Organism
Energy, structure and carbon dioxide: A realistic view of the organism by Ray Peat, PhD “But the philosophy of Causes & Consequences misled Lavater as it has all his Contemporaries. Each thing is its own cause & its own effect.” W. Blake, c. 1788 What could be more important to understand than biological energy? Thought, growth, movement, every philosophical and practical issue involves the nature of biological energy. The question of biological energy is usually handled in the manner of the cosmologist who explained that the earth rests on the back of an elephant; when asked what the elephant stood on, the cosmologist replied that “it’s elephants all the way down.” Several decades ago, it was discovered that ATP mediates many processes in the energized cell, but there is still fundamental disagreement on the question of how ATP is synthesized, and how its energy is used to produce movement, to control the movement of water in cells and organs and to regulate the ionic balance of cells and fluids, and even why its absence produces rigor mortis. When people actually try to examine the question of how the “high energy bond” of ATP can be transformed into usable energy, they sometimes find that it is easier to propose fundamental changes in the laws of physics than to find an explanation within ordinary physics and chemistry. (For example, Physiologie 1986 Jan-Mar;23(1):65-8, “The non- conservation of parity in the domain of elementary particles and a possible mechanism for the delivery of energy from the ATP molecule,” Portelli, C.) More often, biologists simply prefer not to go beyond the first or second elephant. -
Systemic Dissemination of Viral Vectors During Intratumoral Injection
Molecular Cancer Therapeutics 1233 Systemic dissemination of viral vectors during intratumoral injection Yong Wang,1 Jim Kang Hu,2 Ava Krol,1 therapy (1, 2). However, the efficacy of gene therapy is Yong-Ping Li,2 Chuan-Yuan Li,2 and Fan Yuan1 still limited by the delivery of therapeutic genes into 1 2 target cells. Systemic delivery of viral vectors is inade- Departments of Biomedical Engineering and Radiation quate, primarily due to the poor interstitial penetration in Oncology, Duke University, Durham, NC solid tumors (3, 4) and normal tissue toxicity caused by viral vectors and/or gene products (5–15). Different approaches have been developed for reducing Abstract the toxicity in normal tissues. One is to switch to non-viral Intratumoral injection is a routine method for local viral vectors, such as cationic liposomes or polymers (16, 17). gene delivery that may improve interstitial transport of viral Non-viral vectors are less toxic and may have similar vectors in tumor tissues and reduce systemic toxicity. transfection efficiency in vitro as viral vectors. However, However, the concentration of transgene products in non-viral vectors are in general less efficient in vivo. The normal organs, such as in the liver, may still exceed normal second approach is to use tissue targeting viral vectors tissue tolerance if the products are highly toxic. The (18–23), which can be achieved through at least two elevated concentration in normal tissues is likely to be mechanisms. One is to incorporate specific molecular caused by the dissemination of viral vectors from the structures on the vector surface that can bind to unique tumor. -
Active Coacervate Droplets Are Protocells That Grow and Resist Ostwald Ripening
1 Active coacervate droplets are protocells that grow and resist Ostwald ripening 2 3 Karina K. Nakashima1, Merlijn H. I. van Haren1, Alain A. M. André1, Irina Robu1 and 4 Evan Spruijt1* 5 6 1 Institute for Molecules and Materials, Radboud University, Heyendaalseweg 135, 7 6525 AJ Nijmegen, the Netherlands. 8 * 9 Correspondence: [email protected] 10 11 Keywords: protocells, complex coacervates, artificial growth model, active droplets 12 13 Abstract 14 Active coacervate droplets are liquid condensates coupled to a chemical reaction that turns over 15 their components, keeping the droplets out of equilibrium. This turnover can be used to drive active 16 processes such as growth, and provide an insight into the chemical requirements underlying 17 (proto)cellular behaviour. Moreover, controlled growth is a key requirement to achieve population 18 fitness and survival. Here we present a minimal, nucleotide-based coacervate model for active 19 droplets, and report three key findings that make these droplets into evolvable protocells. First, we 20 show that coacervate droplets form and grow by the fuel-driven synthesis of new coacervate 21 material. Second, we find that these droplets do not undergo Ostwald ripening, which we attribute 22 to the attractive electrostatic interactions within complex coacervates, active or passive. Finally, 23 we show that the droplet growth rate reflects experimental conditions such as substrate, enzyme 24 and protein concentration, and that a different droplet composition (addition of RNA) leads to 25 altered growth rates and droplet fitness. These findings together make active coacervate droplets a 26 powerful platform to mimic cellular growth at a single-droplet level, and to study fitness at a 27 population level. -
Proteinoid Nanocapsules for Drug Delivery Applications
polymers Article Engineering of Doxorubicin-Encapsulating and TRAIL-Conjugated Poly(RGD) Proteinoid Nanocapsules for Drug Delivery Applications Elad Hadad 1, Safra Rudnick-Glick 1, Ella Itzhaki 1, Matan Y. Avivi 2, Igor Grinberg 1, Yuval Elias 1 and Shlomo Margel 1,* 1 Department of Chemistry, Institute of Nanotechnology & Advanced Materials, Bar-Ilan University, Ramat Gan 5290002, Israel; [email protected] (E.H.); [email protected] (S.R.-G.); [email protected] (E.I.); [email protected] (I.G.); [email protected] (Y.E.) 2 The Mina and Everard Goodman Faculty of Life Sciences, Institute of Nanotechnology & Advanced Materials, Bar-Ilan University, Ramat Gan 5290002, Israel; [email protected] * Correspondence: [email protected]; Tel.: +972-52-889-8600 Received: 8 October 2020; Accepted: 11 December 2020; Published: 16 December 2020 Abstract: Proteinoids are non-toxic biodegradable polymers prepared by thermal step-growth polymerization of amino acids. Here, P(RGD) proteinoids and proteinoid nanocapsules (NCs) based on D-arginine, glycine, and L-aspartic acid were synthesized and characterized for targeted tumor therapy. Doxorubicin (Dox), a chemotherapeutic drug used for treatment of a wide range of cancers, known for its adverse side effects, was encapsulated during self-assembly to form Dox/P(RGD) NCs. In addition, tumor necrosis factor-related apoptosis-inducing ligand (TRAIL), which can initiate apoptosis in most tumor cells but undergoes fast enzyme degradation, was stabilized by covalent conjugation to hollow P(RGD) NCs. The effect of polyethylene glycol (PEG) conjugation was also studied. Cytotoxicity tests on CAOV-3 ovarian cancer cells demonstrated that Dox/P(RGD) and TRAIL-P(RGD) NCs were as effective as free Dox and TRAIL with cell viability of 2% and 10%, respectively, while PEGylated NCs were less effective.