1 Revised version: November 21st, 2016 2 3 4 Denser mitogenomic sampling improves resolution of the phylogeny of the 5 superfamily Trochoidea (Gastropoda: Vetigastropoda) 6 7 8 Juan E. Uribe1, Suzanne T. Williams2, José Templado1, Samuel 9 Abalde1, and Rafael Zardoya1* 10 11 1Museo Nacional de Ciencias Naturales (MNCN-CSIC), José Gutiérrez 12 Abascal 2, 28006, Madrid, Spain 13 2Department of Life Sciences, Natural History Museum, Cromwell Rd, 14 London SW7 5BD, UK 15 16 17 18 19 20 21 *Correspondence: R. Zardoya; email:
[email protected] 22 23 ABSTRACT 24 The great morphological and ecological diversity within the superfamily Trochoidea s.l. 25 (Gastropoda: Vetigastropoda) has in the past hindered the reconstruction of a robust 26 phylogeny for the group based on morphology. Moreover, previous molecular 27 phylogenies disagreed on the monophyly and internal relationships of Trochoidea s.l., 28 as well as on its relative phylogenetic position within Vetigastropoda. In order to further 29 resolve the trochoidean and vetigastropod phylogenetic trees, we considerably increased 30 the representation of trochoidean families for which no previous mitochondrial (mt) 31 genomes were available: the complete mt genome of Cittarium pica (Tegulidae) and the 32 nearly complete mt genomes of Tectus virgatus (Tegulidae), Gibbula umbilicaris 33 (Trochidae), and Margarites vorticiferus (Margaritidae) were sequenced. In addition, 34 the nucleotide sequences of all protein coding and rRNA genes of Clanculus 35 margaritarius (Trochidae) and of Calliostoma zizyphinum (Calliostomatidae) were 36 derived from transcriptomic sequence data. The reconstructed phylogenetic trees using 37 probabilistic methods and Neomphalina as outgroup recovered with maximal support a 38 Trochoidea sensu Hickman & McLean, 1990 clade that included superfamilies 39 Angarioidea and Phasianelloidea deeply nested within superfamily Trochoidea sensu 40 Williams (2012).