DOCSLIB.ORG

Tesis Doctoral

Total Page：16

File Type：pdf, Size：1020Kb

Download full-text PDF Read full-text
Tesis Doctoral UNIVERSIDAD AUSTRAL DE CHILE FACULTAD DE CIENCIAS AGENTES CONDUCTORES DEL PROCESO DE ECLOSIÓN CAPSULAR: Crepipatella dilatata COMO MODELO DE ESTUDIO TESIS DOCTORAL PAOLA VIVIANA ANDRADE VILLAGRAN VALDIVIA – CHILE 2016 AGENTES CONDUCTORES DEL PROCESO DE ECLOSIÓN CAPSULAR: Crepipatella dilatata COMO MODELO DE ESTUDIO Tesis presentada a la Facultad de Ciencias de la Universidad Austral de Chile en cumplimiento parcial de los requisitos para optar al grado de Doctor en Ciencias por PAOLA VIVIANA ANDRADE VILLAGRAN Valdivia - Chile 2016 Dedicada a mi pequeña Leonor AGRADECIMIENTOS Quiero agradecer a todas las personas que de alguna u otra forma han aportado en el desarrollo de mi tesis. Dar las gracias a mi familia por el apoyo y la paciencia. También quisiera agradecer a las personas que trabajan en el laboratorio y a mis compañeros del Doctorado, por hacer siempre mas agradable y entretenida mi estadia en la universidad. Agradecer a mi profesor patrocinante Dr. Oscar Chaparro, por el apoyo, la paciencia y los consejos durante todos estos años de estudio. A mi comisión evaluadora, Dr. Antonio Brante, Dr. Kurt Paschke, Dr. Jorge Navarro, por participar en el proceso de evaluación. Dar las gracias al Doctor Luis Vargas por permitirme hacer uso de su laboratorio y a Ricardo por ayudarme con los analisis bioquímicos. Gracias a Conicyt por la beca de Doctorado nacional, que me permitió dedicarme con tranquilidad al Doctorado. A la beca de gastos operacionales 21120654 otorgada por Conicyt para el desarrollo de la tesis. También agradecer a la Dirección de Postgrado, Dirección de Investigación y Desarrollo, Escuela de Graduados de la Facultad de Ciencias de la Universidad Austral de Chile, por el apoyo económico otorgado durante mi asistencia a diferentes Congresos. Finalmente agradecer al proyecto Fondecyt 1141052 “Hatching process in brooding molluscs: More complex that thougth before”, otorgado al Doctor Oscar Chaparro , por las facilidades logisticas y operacionales otorgadas durante el desarrollo de mi tesis de Doctorado. INDICE GENERAL INDICE GENERAL I INDICE DE TABLAS IV INDICE DE FIGURAS V RESUMEN XI ABSTRACT XIV INTRODUCCION GENERAL 1 CAPITULO 1: WHO CONTROLS HATCHING IN THE BROODING ESTUARINE GASTROPOD Crepipatella dilatata? 9 SUMMARY 9 INTRODUCTION 10 MATERIAL AND METHODS 13 RESULTS 19 DISCUSSION 22 I TABLES 28 FIGURES 30 REFERENCES 40 CAPITULO 2: ACCIÓN BIOMECÁNICA Y OSMÓTICA: POSIBLES MECANISMOS DE ECLOSIÓN CAPSULAR EN Crepipatella dilatata 46 RESUMEN 46 INTRODUCCIÓN 47 METODOLOGÍA 51 RESULTADOS 58 DISCUSIÓN 62 TABLAS 69 FIGURAS 70 REFERENCIAS 82 II CAPITULO 3: ACCIÓN ENZIMÁTICA INTRACAPSULAR COMO MECANISMO DE ECLOSIÓN EN EL GASTRÓPODO INCUBADOR Crepipatella dilatata 89 RESUMEN 89 INTRODUCCIÓN 90 METODOLOGÍA 94 RESULTADOS 98 DISCUSIÓN 100 FIGURAS 106 REFERENCES 110 DISCUSION GENERAL Y CONCLUSIONES 116 REFERENCIAS (introducción y discusión general) 124 III INDICE DE TABLAS CAPITULO 1 Table 1. Crepipatella dilatata. Nested Anova performed for the effect of hatching time (initial and final) nested in the different egg-brood on the average number of juveniles, total number of offspring, shell length of juvenile, and the offspring biomass in each hatched capsule. Bold p-values indicate statistical significance. 28 Table 2. Crepipatella dilatata. Mean content characteristics of capsules hatched (n = 295) and nonhatched (n = 34) (mean ± SD). Different letter means significant differences for each variable between hatched and non-hatched capsules. 29 CAPITULO 2 Tabla 1. Crepipatella dilatata. Promedio de la longitud de concha de juveniles pre-eclosión (n = 4 capsulas), larvas veligeras (n = 5 capsulas) y larvas veligeras donde no se identificó la presencia de rádula (n = 4 capsulas), y tamaño promedio de sus respectivas rádulas. Valores promedio ± desviación estandar. 69 IV INDICE DE FIGURAS CAPITULO 1 Fig. 1 Crepipatella dilatata. Degree of synchronization in hatching among egg capsules within a brood. Y-axis shows the time between hatching of the first egg capsule in a brood and the last egg capsule in the same egg mass. The broods considered in the graph correspond to those in which at least 50% of the capsules had hatched. n = 23 broods. HT: hatching time, NC: number of capsules. 30 Fig. 2 Crepipatella dilatata. A) Mean number of metamorphosed juveniles and C) total offspring (veliger and juveniles) per capsule, hatched at the beginning and at the end of the hatching period from each individual egg mass (n = offspring from 33 egg capsules). Initial hatching (full circles) represent the 25% of those capsules hatched at the beginning and the open circles represent the 25% of those at the end of the hatching period for each egg mass (brood). Mean of juveniles (B) and total offspring (D) in hatched capsules at the beginning and at the end of hatching period for a 33 different egg masses. Different letter on bars show significant differences. Lines on bar show SD. 31 Fig. 3 Crepipatella dilatata. (A) Mean shell length of the metamorphosed juveniles and (C) mean offspring biomass from capsules hatched at the beginning and at the end of the hatching period for each egg mass (n = 33 capsules). Initial hatching (full circles) represent the 25% of those capsules hatched earlier and the open circles represent the 25% of the last hatched capsules in relation of the total time spent during the hatching process for each egg mass. Mean juvenile shell length (B) and mean offspring biomass (D) in capsules hatched at the beginning and at the end of the hatching period for the 33 egg masses. Different letter on bars show significant differences. Lines on bar show SD. 33 V Fig. 4 Crepipatella dilatata. Relationship between developmental stage (metamorphosed, not yet metamorphosed) and egg capsule status (hatched, not yet hatched) on juvenile mean shell length. Bars represent the mean shell length of metamorphosed juveniles (n = 293 egg capsules) and non-metamorphosed veligers (n = 77 egg capsules) from hatched capsules and metamorphosed juveniles (n = 34 egg capsules) and non-metamorphosed veligers (n = 24 egg capsule) from non-hatched capsules. Vertical lines above bars indicate SD and different letters signify significant differences between means. 35 Fig. 5 Crepipatella dilatata. Total number of brooded offspring per capsule and its relation with (A) juvenile shell length at metamorphosis (n = offspring from 293 egg capsules) and (B) offspring brooded biomass (n = offspring from 283 egg capsules) recorded in hatching capsules. SLJ: Shell length of juveniles, OBB: Offspring brooded biomass, TNO: Total number of offspring. Each dot represents the mean value from one capsule. 36 Fig. 6 Crepipatella dilatata. Relationship between mean shell length of metamorphosed juveniles, mean number of metamorphosed juveniles, and mean number of nurse eggs remaining for hatched (n = 283) and non-hatched (n = 34) egg capsules, respectively. 37 Fig. 7 Crepipatella dilatata. Effect of capsule mates on the timing of hatching over 7 days. A) Percentage of capsules opening from different broods, kept individually (n = 59 capsules from 11 broods) or grouped (n = 59 capsules from 11 broods, 2-10 capsules form each brood) with their capsule mates. B) Mean percentage of hatching in all capsules kept individually or grouped. * = non-hatched capsule. Different letters mean significant differences. Bars represent SD. 38 Fig. 8 Crepipatella dilatata. A) Estimated time of maternal investment in the capsular laying process and time registered of hatching for each brood. The broods controlled were those in which at least 50% of capsules had hatched. The capsular laying time for each female was VI estimated multiplying the average time used in laying one capsule (see M & M) by the total hatched capsule number in each experimental brood. B) Mean time (h) spend during capsular laying and the hatching process in each capsule brood (n = 23). Numbers above the bars represent the number of capsules in each brood. Different letters mean significant differences. Bars mean SD. 39 CAPITULO 2 Fig. 1 Crepipatella dilatata. A) Rádula de juvenil pre-eclosión provenientes desde capsulas con desarrollo embrionario en estadío avanzado. B) Juvenil pre-eclosión. 70 Fig. 2 Crepipatella dilatata. Relación entre la longitud de concha promedio de larvas y juveniles encapsulados, con la longitud de rádula promedio. Círculos negros corresponden a juveniles pre-eclosión (n = 42 juveniles en total provenientes de 4 capsulas), círculos grises indican larvas veligeras (n = 23 veligeras en total provenientes de 5 capsulas), y círculos blancos representa larvas veligeras donde no se observó rádula (n = 52 veligeras provenientes de 4 capsulas). Cada capsula proviene de una postura diferente. Barras indican SD. 71 Fig. 3 Crepipatella dilatata. A) Microfotografía de MEB mostrando la zona de eclosión interna de una capsula con veligeras en estadio de desarrollo intermedio. ZH indica zona de eclosión. B) Zona de eclosión maximizada de acuerdo a Fig.3 A. Ancho de rádula de las veligeras encapsuladas corresponde a 33 µm. 72 Fig.4 Crepiaptella dilatata. Microfotografía de MEB en A) Zona de eclosión interna de una capsula con estadío de desarrollo embrionario final (pre-eclosión). HZ indica zona de eclosión. B) zona de eclosión maximizada de acuerdo a Fig.4 A. Ancho promedio de rádula de los juveniles pre-eclosión corresponde a 40 µm. 73 VII Fig. 5 Crepipatella dilatata. Microfotografía de MEB en A) Zona de eclosión interna de capsulas eclosionadas. B) zona de eclosión interna maximizada de acuerdo a Fig. 5 A. HZ indica zona de eclosión. Ancho de rádula de juveniles corresponde a 40 µm. 74 Fig. 6 Crepipatella dilatata. Microfotografía de MEB en zona de eclosión externa de la capsula de C. dilatata. A) Exterior de capsula con embriones en estadio de desarrollo avanzado (juveniles pre-eclosión). B) Detalle de la zona de eclosión externa en capsulas con embriones con nivel de desarrollo avanzado. C) Exterior de capsula eclosionada. D) Detalle de zona de eclosión externa en capsulas eclosionadas. HZ indica zona de eclosión. 75 Fig. 7 Crepipatella dilatata. A) Concentración osmótica del fluido obtenidos de capsulas con diferentes niveles de desarrollo de los embriones encapsulados.
Load more

Recommended publications
  • Research Article Early Development of Monoplex Pilearis
    1 Research Article 2 Early Development of Monoplex pilearis and Monoplex parthenopeus (Gastropoda: 3 Cymatiidae) - Biology and Morphology 4 5 Ashlin H. Turner*, Quentin Kaas, David J. Craik, and Christina I. Schroeder* 6 7 Institute for Molecular Bioscience, The University of Queensland, Brisbane, 4072, Qld, Australia 8 9 *Corresponding authors: 10 Email: [email protected], phone: +61-7-3346-2023 11 Email: [email protected], phone: +61-7-3346-2021 1 12 Abstract 13 Members of family Cymatiidae have an unusually long planktonic larval life stage (veligers) which 14 allows them to be carried within ocean currents and become distributed worldwide. However, little 15 is known about these planktonic veligers and identification of the larval state of many Cymatiidae 16 is challenging at best. Here we describe the first high-quality scanning electron microscopy images 17 of the developing veliger larvae of Monoplex pilearis and Monoplex parthenopeus (Gastropoda: 18 Cymatiidae). The developing shell of Monoplex veligers was captured by SEM, showing plates 19 secreted to form the completed shell. The incubation time of the two species was recorded and 20 found to be different; M. parthenopeus took 24 days to develop fully and hatch out of the egg 21 capsules, whereas M. pilearis took over a month to leave the egg capsule. Using scanning electron 22 microscopy and geometric morphometrics, the morphology of veliger larvae was compared. No 23 significant differences were found between the shapes of the developing shell between the two 24 species; however, it was found that M. pilearis was significantly larger than M.
    [Show full text]
  • Diversity of Benthic Marine Mollusks of the Strait of Magellan, Chile
    ZooKeys 963: 1–36 (2020) A peer-reviewed open-access journal doi: 10.3897/zookeys.963.52234 DATA PAPER https://zookeys.pensoft.net Launched to accelerate biodiversity research Diversity of benthic marine mollusks of the Strait of Magellan, Chile (Polyplacophora, Gastropoda, Bivalvia): a historical review of natural history Cristian Aldea1,2, Leslie Novoa2, Samuel Alcaino2, Sebastián Rosenfeld3,4,5 1 Centro de Investigación GAIA Antártica, Universidad de Magallanes, Av. Bulnes 01855, Punta Arenas, Chile 2 Departamento de Ciencias y Recursos Naturales, Universidad de Magallanes, Chile 3 Facultad de Ciencias, Laboratorio de Ecología Molecular, Departamento de Ciencias Ecológicas, Universidad de Chile, Santiago, Chile 4 Laboratorio de Ecosistemas Marinos Antárticos y Subantárticos, Universidad de Magallanes, Chile 5 Instituto de Ecología y Biodiversidad, Santiago, Chile Corresponding author: Sebastián Rosenfeld ([email protected]) Academic editor: E. Gittenberger | Received 19 March 2020 | Accepted 6 June 2020 | Published 24 August 2020 http://zoobank.org/9E11DB49-D236-4C97-93E5-279B1BD1557C Citation: Aldea C, Novoa L, Alcaino S, Rosenfeld S (2020) Diversity of benthic marine mollusks of the Strait of Magellan, Chile (Polyplacophora, Gastropoda, Bivalvia): a historical review of natural history. ZooKeys 963: 1–36. https://doi.org/10.3897/zookeys.963.52234 Abstract An increase in richness of benthic marine mollusks towards high latitudes has been described on the Pacific coast of Chile in recent decades. This considerable increase in diversity occurs specifically at the beginning of the Magellanic Biogeographic Province. Within this province lies the Strait of Magellan, considered the most important channel because it connects the South Pacific and Atlantic Oceans. These characteristics make it an interesting area for marine research; thus, the Strait of Magellan has histori- cally been the area with the greatest research effort within the province.
    [Show full text]
  • The Slipper Snail, Crepidula: an Emerging Lophotrochozoan Model System†
    Reference: Biol. Bull. 218: 211–229. (June 2010) © 2010 Marine Biological Laboratory The Slipper Snail, Crepidula: An Emerging Lophotrochozoan Model System† JONATHAN J. HENRY1,* RACHEL COLLIN2, AND KIMBERLY J. PERRY1 1University of Illinois, Department of Cell & Developmental Biology, 601 S. Goodwin Ave, Urbana, Illinois 61801; and 2Smithsonian Tropical Research Institute, Box 0843-03092, Balboa, Republic of Panama Abstract. Recent developmental and genomic research al., 1995; Nielsen, 2001; Mallatt and Winchell, 2002; Pas- focused on “slipper snails” in the genus Crepidula has samaneck and Halanych, 2006; Dunn et al., 2008; Paps et positioned Crepidula fornicata as a de facto model system al., 2009). The relationships among the lophotrochozoan for lophotrochozoan development. Here we review recent taxa are still somewhat poorly resolved (Dunn et al., 2008; developments, as well as earlier reports demonstrating the Paps et al., 2009), but the core group (lophotrochozoan widespread use of this system in studies of development and sensu stricto of Paps et al., 2009) includes molluscs, anne- life history. Recent studies have resulted in a well-resolved lids, nemertines, the lophophorates, platyhelminths, and fate map of embryonic cell lineage, documented mecha- other smaller phyla). Molecular phylogenies sometimes re- nisms for axis determination and D quadrant specification, cover other groups such as rotifers and gastrotrichs nested preliminary gene expression patterns, and the successful within the core lophotrochozoans (e.g., Passamaneck and application of loss- and gain-of-function assays. The recent Halanych, 2006), although these groups sometimes fall out- development of expressed sequence tags and preliminary side, as sisters to the core group taxa (e.g., Paps et al., genomics work will promote the use of this system, partic- 2009).
    [Show full text]
  • A New Pliocene Mollusk Fauna from Mejillones, Northern Chile Sven N
    A new Pliocene mollusk fauna from Mejillones, northern Chile Sven N. Nielsen Paläontologische Zeitschrift Scientific Contributions to Palaeontology ISSN 0031-0220 Volume 87 Number 1 Paläontol Z (2013) 87:33-66 DOI 10.1007/s12542-012-0146-0 1 23 Your article is protected by copyright and all rights are held exclusively by Springer- Verlag. This e-offprint is for personal use only and shall not be self-archived in electronic repositories. If you wish to self-archive your work, please use the accepted author’s version for posting to your own website or your institution’s repository. You may further deposit the accepted author’s version on a funder’s repository at a funder’s request, provided it is not made publicly available until 12 months after publication. 1 23 Author's personal copy Pala¨ontol Z (2013) 87:33–66 DOI 10.1007/s12542-012-0146-0 RESEARCH PAPER A new Pliocene mollusk fauna from Mejillones, northern Chile Sven N. Nielsen Received: 12 March 2012 / Accepted: 13 June 2012 / Published online: 5 July 2012 Ó Springer-Verlag 2012 Abstract A new Pliocene (3.4 Ma) mollusk fauna from (Vermeij and DeVries, 1997), Austrofusus steinmanni Mejillones Peninsula, northern Chile is described and (Mo¨ricke, 1896) und Leukoma antiqua (King, 1832). Fu¨r compared with the Pliocene La Cueva fauna of little con- verschiedene Arten werden die a¨ltesten Nachweise und strained age from central Chile and some species from the geographische Reichweiten erweitert. Ein gemeinsames Huenteguapi Sandstone overlying the Ranquil Formation Vorkommen von Warmwasserarten, welche dem MIS 11 on Arauco Peninsula, south central Chile.
    [Show full text]
  • Includes Abstracts of the
    Number 65 (August 2015) The Malacologist Page 1 NUMBER 65 AUGUST 2015 Contents EDITORIAL …………………………….. ............................2 ANNUAL GENERAL MEETING—SPRING 2015 Annual Report of Council ...........................................................21 NOTICES ………………………………………………….2 Election of officers ………………………………………….....24 RESEARCH GRANT REPORTS Molecular phylogeny of Chaetodermomorpha (=Caudofoveata) EUROMOL CONFERENCE Programme in retrospect ……………………………………….….25 (Mollusca). Conference Abstracts - Oral presentations………………….....26 Nina Mikkelsen …………………………….………………..4 - Poster presentations ……………...…..53 The Caribbean shipworm Teredothyra dominicensis (Bivalvia, Teredinidae) has invaded and established breeding populations FORTHCOMING MEETINGS …………………………….…..... 72 in the Mediterranean Sea. Molluscan Forum .......................................................................72 J. Reuben Shipway, Luisa Borges, Johann Müller GRANTS AND AWARDS OF THE SOCIETY.............................76 & Simon Cragg ……………………………………………….7 MEMBERSHIP NOTICES ………………………………………....77 ANNUAL AWARD Evolution of chloroplast sequestration in Sacoglossa (Gastropoda, Heterobranchia) Gregor Christa ...……………………………………………....10 AGM CONFERENCE Programme in retrospect Planktic Gastropods ……………...….12 Conference Abstracts - Oral presentations………………….....13 - Poster presentations …………….....…18 Includes abstracts of the .. Images from The heart of a dragon: extraordinary circulatory system of the scaly-foot gastropod revealed Chong Chen, Jonathan Copley, Katrin Linse,
    [Show full text]
  • Los Invertebrados Marinos
    LOS INVERTEBRADOS MARINOS Editor responsable Javier A. Calcagno LOS INVERTEBRADOS MARINOS VAZQUEZ MAZZINI EDITORES Fundación de Historia Natural Félix de Azara Departamento de Ciencias Naturales y Antropológicas CEBBAD - Instituto Superior de Investigaciones Universidad Maimónides Hidalgo 775 - 7° piso (1405BDB), Ciudad Autónoma de Buenos Aires, República Argentina. Teléfonos: 011-4905-1100 (int. 1228) E-mail: [email protected] Página web: www.fundacionazara.org.ar Editor responsable: Javier A. Calcagno Foto de Tapa: Sergio Massaro Fotos de contratapa: Foto de la izquierda, Gentileza de Agustín Schiariti Las otras fotos son Gentileza de Cota Cero Buceo (Las Grutas, Río Negro) Realización, diseño y producción gráfica: José Luis Vázquez, Fernando Vázquez Mazzini y Cristina Zavatarelli Vázquez Mazzini Editores [email protected] www.vmeditores.com.ar Re ser va dos los de re chos pa ra to dos los paí ses. Nin gu na par te de es ta pu bli ca ción, in cluido el di se ño de la cu bier ta, pue de ser re pro du ci da, al ma ce na da, o trans mi ti da de nin gu na for ma, ni por nin gún me dio, sea es te electró ni co, quí- mi co, me cá ni co, electro-óp ti co, gra ba ción, fo to co pia, CD Rom, In ter net, o cual quier otro, sin la pre via au to ri za ción es cri ta por par te de la edi to rial. Este trabajo refleja exclusivamente las opiniones profesionales y científicas de los autores y no es responsabilidad de la editorial el contenido de la presente obra.
    [Show full text]
  • Large-Scale Gene Flow in the Barnacle Jehlius Cirratus and Contrasts with Other Broadly-Distributed Taxa Along the Chilean Coast
    Large-scale gene flow in the barnacle Jehlius cirratus and contrasts with other broadly-distributed taxa along the Chilean coast Baoying Guo1 and John P. Wares2 1 College of Marine Science and Technology, Zhejiang Ocean University, Zhoushan, Zhejiang, China 2 Department of Genetics and Odum School of Ecology, University of Georgia, Athens, GA, USA ABSTRACT We evaluate the population genetic structure of the intertidal barnacle Jehlius cirratus across a broad portion of its geographic distribution using data from the mitochondrial cytochrome oxidase I (COI) gene region. Despite sampling diversity from over 3,000 km of the linear range of this species, there is only slight regional structure indicated, with overall 8CT of 0.036 (p < 0:001) yet no support for isolation by distance. While these results suggest greater structure than previous studies of J. cirratus had indicated, the pattern of diversity is still far more subtle than in other similarly-distributed species with similar larval and life history traits. We compare these data and results with recent findings in four other intertidal species that have planktotrophic larvae. There are no clear patterns among these taxa that can be associated with intertidal depth or other known life history traits. Subjects Biodiversity, Ecology, Evolutionary Studies, Marine Biology, Zoology Keywords Barnacle, Chile, Phylogeography, Population genetics, Biogeography INTRODUCTION Submitted 26 September 2016 A persistent question in marine biogeography and population biology involves the Accepted 9 January 2017 Published 7 February 2017 interaction of species life history, geographic range, and trait or genealogical diversity within that range. In some cases, genealogical diversity or ``structure'' (Wares, 2016) within Corresponding author John P.
    [Show full text]
  • LIST of AQUATIC ALIEN SPECIES of the IBERIAN PENINSULA (2020) Updated List of Aquatic Alien Species Introduced and Established in Iberian Inland Waters
    Red-eared slider (Trachemys scripta) © Javier Murcia Requena LIST OF AQUATIC ALIEN SPECIES OF THE IBERIAN PENINSULA (2020) Updated list of aquatic alien species introduced and established in Iberian inland waters Authors Oliva-Paterna F.J., Ribeiro F., Miranda R., Anastácio P.M., García-Murillo P., Cobo F., Gallardo B., García-Berthou E., Boix D., Medina L., Morcillo F., Oscoz J., Guillén A., Aguiar F., Almeida D., Arias A., Ayres C., Banha F., Barca S., Biurrun I., Cabezas M.P., Calero S., Campos J.A., Capdevila-Argüelles L., Capinha C., Carapeto A., Casals F., Chainho P., Cirujano S., Clavero M., Cuesta J.A., Del Toro V., Encarnação J.P., Fernández-Delgado C., Franco J., García-Meseguer A.J., Guareschi S., Guerrero A., Hermoso V., Machordom A., Martelo J., Mellado-Díaz A., Moreno J.C., Oficialdegui F.J., Olivo del Amo R., Otero J.C., Perdices A., Pou-Rovira Q., Rodríguez-Merino A., Ros M., Sánchez-Gullón E., Sánchez M.I., Sánchez-Fernández D., Sánchez-González J.R., Soriano O., Teodósio M.A., Torralva M., Vieira-Lanero R., Zamora-López, A. & Zamora-Marín J.M. LIFE INVASAQUA – TECHNICAL REPORT LIFE INVASAQUA – TECHNICAL REPORT Pumpkinseed (Lepomis gibbosus) © Bernard Dupont.. CC-BY-SA-2.0 5 LIST OF AQUATIC ALIEN SPECIES OF THE IBERIAN PENINSULA (2020) Updated list of aquatic alien species introduced and established in Iberian inland waters LIFE INVASAQUA - Aquatic Invasive Alien Species of Freshwater and Estuarine Systems: Awareness and Prevention in the Iberian Peninsula. LIFE17 GIE/ES/000515 This publication is a Technical report by the European Project LIFE INVASAQUA (LIFE17 GIE/ES/000515).
    [Show full text]
  • Molluscan Studies Journal of Molluscan Studies (2018): 1–11
    Journal of The Malacological Society of London Molluscan Studies Journal of Molluscan Studies (2018): 1–11. doi:10.1093/mollus/eyy045 Downloaded from https://academic.oup.com/mollus/advance-article-abstract/doi/10.1093/mollus/eyy045/5105852 by guest on 22 September 2018 Enveloping walls, encapsulated embryos and intracapsular fluid: changes during the early development stages in the gastropod Acanthina monodon (Muricidae) J.A. Büchner-Miranda1, R.J. Thompson2, L.M. Pardo1,3, H. Matthews-Cascon4,5, L.P. Salas-Yanquin1, P.V. Andrade-Villagrán1,6 and O.R. Chaparro1 1Instituto de Ciencias Marinas y Limnológicas, Universidad Austral de Chile, Valdivia, Chile; 2Ocean Sciences Centre, Memorial University, St John’s, NL A1C 5S7, Canada; 3Centro FONDAP de Investigación en Dinámica de Ecosistemas Marinos de Altas Latitudes (IDEAL), Universidad Austral de Chile, Valdivia, Chile; 4Laboratório de Invertebrados Marinhos, Departamento de Biologia, Centro de Ciências, Universidade Federal do Ceará, Fortaleza, Brasil; 5Instituto de Ciências do Mar, Universidade Federal do Ceará, Fortaleza, Brasil; and 6Centro de Investigación en Biodiversidad y Ambientes Sustentables (CIBAS), Facultad de Ciencias, Universidad Católica de la Santísima Concepción, Concepción, Chile Correspondence: O.R. Chaparro; e-mail: [email protected] (Received 28 February 2018; editorial decision 14 August 2018) ABSTRACT Encapsulation of embryos in marine gastropods affords protection for the developing young, whether or not parental care takes place. The capsule wall is laminated and its dimensions change during develop- ment. Dissolution of the capsule wall releases dissolved organic matter (DOM) into the intracapsular fluid, providing a nutritional source for the embryo. The capsule wall of Acanthina monodon is composed of three layers, a thin outer layer with projections to the exterior, a thicker intermediate layer containing vacuoles and a thin inner layer that gives rise to the hatching plug.
    [Show full text]
  • Confirmation of the Identification and Establishment of the South American Slipper Limpet Crepipatella Dilatata (Lamark 1822) (C
    Aquatic Invasions (2009) Volume 4, Issue 2: 377-380 DOI 10.3391/ai.2009.4.2.13 © 2009 The Author(s) Journal compilation © 2009 REABIC (http://www.reabic.net) This is an Open Access article Short communication Confirmation of the identification and establishment of the South American slipper limpet Crepipatella dilatata (Lamark 1822) (Caenogastropoda: Calyptraeidae) in Northern Spain Rachel Collin1*, Paul Farrell2 and Simon Cragg2 1Smithsonian Tropical Research Institute, Apartado Postal 0843-03092, Balboa, Ancon, Republic of Panama E-mail: [email protected] 2School of Biological Sciences, Institute of Marine Sciences, Ferry Road, Portsmouth P04 9LY, UK E-mails: [email protected], [email protected] *Corresponding author Received 22 February 2009; accepted in revised form 16 March 2009; published online 3 April 2009 Abstract Calyptraeid gastropods have been introduced frequently in bays and ports around the world, and have become rampantly invasive in several cases. Here we confirm the identification and establishment of a recently-detected population of Crepipatella in northern Spain. Because their shells do not have many diagnostic features, introductions of calyptraeids are often accompanied by confusion about the identity and therefore origin of the species in question. We use DNA sequence data and developmental observations to verify the species identity of this population as the South American species Crepipatella dilatata. The apparently rapid spread of this species, which lacks a larval stage, may be due to human action. Key words: Galicia, Crepidula, barcode, COI, direct development, exotic species Several species of calyptraeid gastropods and is now widespread in Korea, Japan, and (slipper limpets, cup-and-saucer limpets, and hat Hong Kong (Choe and Park 1992; Ekawa 1985; limpets) have been introduced and established Morton 1987).
    [Show full text]
  • A New Species of Crepipatella (Gastropoda: Calyptraeidae) from Northern Chile
    Molluscan Research 32(3): 145–153 ISSN 1323-5818 http://www.mapress.com/mr/ Magnolia Press A new species of Crepipatella (Gastropoda: Calyptraeidae) from northern Chile DAVID VELIZ1, FEDERICO M. WINKLER2, CHITA GUISADO3 & RACHEL COLLIN4 1Departamento de Ciencias Ecológicas and Instituto de Ecología y Biodiversidad (IEB), Universidad de Chile. Casilla 653, Santiago, Chile. E-mail: [email protected] (corresponding author) 2 Departamento de Biología Marina, Universidad Católica del Norte, Sede Coquimbo. Casilla 117, Coquimbo, Chile. E-mail: [email protected] 3 Facultad de Ciencias del Mar y Recursos Naturales, Universidad de Valparaíso. Casilla 5080, Reñaca, Viña del Mar, Chile. E-mail: [email protected] 4 Smithsonian Tropical Research Institute, Apartado Postal 0843-03092, Balboa, Ancon, República de Panamá. E-mail: [email protected] Abstract Crepipatella occulta n. sp. is described from the intertidal zone in northern Chile. This species is morphologically cryptic with two other Crepipatella species from Chile, Crepipatella dilatata (Lamarck, 1822) and Crepipatella peruviana (Lamarck, 1822) (a senior synonym of C. fecunda), with respect to adult shell morphology and anatomy. However, Crepipatella occulta is clearly distinguishable from both of them on the basis of embryonic development. It can be distinguished from Crepipatella peruviana, a planktotroph, and Crepipatella dilatata, a direct developer with uncleaved nurse eggs, because it has direct devel- opment with developing nurse embryos that are consumed before the juveniles hatch. Genetic data from DNA sequences also support the distinct status of this species, and show that the South African species C. capensis (Quoy & Gaimard, 1832–33) is more closely related to C. dilatata and C.
    [Show full text]
  • Reproductive Biology of the Encapsulating, Brooding Gastropod Crepipatella Dilatata Lamarck (Gastropoda, Calyptraeidae)
    RESEARCH ARTICLE Reproductive biology of the encapsulating, brooding gastropod Crepipatella dilatata Lamarck (Gastropoda, Calyptraeidae) 1 1,2 3 1,4 Oscar R. ChaparroID *, VõÂctor M. Cubillos , Jaime A. Montory , Jorge M. Navarro , Paola V. Andrade-VillagraÂn5 1 Instituto de Ciencias Marinas y LimnoloÂgicas, Universidad Austral de Chile, Valdivia, Chile, 2 Laboratorio de Recursos AcuaÂticos y Costeros de Calfuco, Universidad Austral de Chile, Valdivia, Chile, 3 Centro i~mar, Universidad de Los Lagos, Puerto Montt, Chile, 4 Centro Fondap de InvestigacioÂn DinaÂmica de Ecosistemas Marinos de Altas Latitudes (IDEAL), Universidad Austral de Chile, Valdivia, Chile, 5 Centro de InvestigacioÂn a1111111111 en Biodiversidad y Ambientes Sustentables (CIBAS), Facultad de Ciencias, Universidad CatoÂlica de la a1111111111 SantõÂsima ConcepcioÂn, ConcepcioÂn, Chile a1111111111 a1111111111 * [email protected] a1111111111 Abstract Among calyptraeid gastropods, males become females as they get older, and egg capsules OPEN ACCESS containing developing embryos are maintained beneath the mother's shell until the encap- Citation: Chaparro OR, Cubillos VM, Montory JA, sulated embryos hatch. Crepipatella dilatata is an interesting biological model considering  Navarro JM, Andrade-Villagran PV (2019) that is an estuarine species and thus periodically exposed to elevated environment-physio- Reproductive biology of the encapsulating, brooding gastropod Crepipatella dilatata Lamarck logical pressures. Presently, there is not much information about the reproductive biology (Gastropoda, Calyptraeidae). PLoS ONE 14(7): and brooding parameters of this gastropod. This paper describes field and laboratory obser- e0220051. https://doi.org/10.1371/journal. vations monitoring sex changes, brooding frequencies, sizes of brooding females, egg pone.0220051 mass characteristics, and embryonic hatching conditions. Our findings indicate that C.
    [Show full text]