Supplemental Table 1. Genes with at Least 2-Fold Change After 24 H and 72 H Incubation with PD- 0332991 Gene Symbol Description

Supplemental Table 1. Genes with At Least 2-Fold Change After 24 h and 72 h Incubation with PD- 0332991 AsPC-1 COLO-357

Gene Symbol Description Fold Change Fold Change

24 h 72 h 24 h 72 h CDT1 Homo sapiens chromatin licensing 0.38 0.95 0.39 0.86 and DNA replication factor 1 HMGB2 Homo sapiens high-mobility group 0.36 0.69 0.16 0.37 box 2 FANCI Homo sapiens Fanconi anemia, 0.48 0.98 0.45 0.8 complementation group I STMN1 Homo sapiens stathmin 1 (STMN1) 0.42 0.82 0.23 0.53 FAM116B Homo sapiens family with sequence 2.08 0.73 2 1.58 similarity 116, member B RFC5 Homo sapiens replication factor C 0.5 0.76 0.46 0.58 (activator 1) MCM7 Homo sapiens minichromosome 0.47 0.85 0.48 0.53 maintenance complex component 7 CDC20 Homo sapiens cell division cycle 20 0.5 0.84 0.37 0.56 homolog (S. cerevisiae) (CDC20) NDC80 Homo sapiens NDC80 homolog, 0.45 0.8 0.4 0.69 kinetochore complex component CENPF Homo sapiens centromere protein 0.41 0.81 0.3 0.82 F, 350/400ka (mitosin) TUBB Homo sapiens tubulin, beta 0.5 0.89 0.5 0.72 CDKN3 Homo sapiens cyclin-dependent 0.31 0.66 0.32 0.42 kinase inhibitor 3 CDC45L Homo sapiens CDC45 cell division 0.36 0.96 0.38 0.49 cycle 45-like (S. cerevisiae) RAD51AP1 Homo sapiens RAD51 associated 0.4 0.97 0.34 0.58 protein 1 PSRC1 Homo sapiens proline/serine-rich 0.3 0.7 0.18 0.53 coiled-coil 1 PBK Homo sapiens PDZ binding kinase 0.35 0.95 0.44 0.75 EXO1 Homo sapiens exonuclease 1 0.35 0.99 0.36 0.57 (EXO1) MGC40489 PREDICTED: Homo sapiens 0.37 0.92 0.41 0.69 hypothetical protein MGC40489 RRM2 Homo sapiens ribonucleotide 0.29 1.13 0.29 0.83 reductase M2 polypeptide CDCA5 Homo sapiens cell division cycle 0.44 0.97 0.49 0.73 associated 5 AURKB Homo sapiens aurora kinase B 0.26 0.75 0.2 0.52 KIF2C Homo sapiens kinesin family 0.35 0.76 0.26 0.55 member 2C TOP2A Homo sapiens topoisomerase 0.24 0.82 0.24 0.68 (DNA) II alpha 170kDa PCNA Homo sapiens proliferating cell 0.42 0.92 0.45 0.69 nuclear antigen ASF1B Homo sapiens ASF1 anti-silencing 0.36 0.86 0.38 0.82 function 1 homolog B KIF20A Homo sapiens kinesin family 0.18 0.52 0.11 0.43 member 20A (KIF20A)

POLA2 Homo sapiens polymerase (DNA 0.48 0.91 0.49 0.7 directed), alpha 2 (70kD subunit)

TTC14 Homo sapiens tetratricopeptide 2.27 2.04 2.27 1.67 repeat domain 14

TROAP Homo sapiens trophinin associated 0.37 0.65 0.23 0.5 protein (tastin)

C9orf140 Homo sapiens chromosome 9 open 0.44 0.57 0.42 0.54 reading frame 140 HJURP Homo sapiens Holliday junction 0.25 0.73 0.24 0.7 recognition protein CDCA8 Homo sapiens cell division cycle 0.38 0.84 0.45 0.82 associated 8 FBXO5 Homo sapiens F-box protein 5 0.39 0.87 0.35 0.59 CDC25A Homo sapiens cell division cycle 25 0.41 1.11 0.4 0.79 homolog A (S. pombe) UBE2T Homo sapiens ubiquitin-conjugating 0.39 0.86 0.38 0.58 enzyme E2T (putative) CCNB1 Homo sapiens cyclin B1 0.32 0.81 0.27 0.57 UBE2C Homo sapiens ubiquitin-conjugating 0.35 0.85 0.2 0.55 enzyme E2C (UBE2C) CENPE Homo sapiens centromere protein 0.41 0.84 0.29 0.64 E, 312kDa GMNN Homo sapiens geminin, DNA 0.47 0.9 0.47 0.59 replication inhibitor KPNA2 Homo sapiens karyopherin alpha 2 0.34 0.89 0.37 0.66 (RAG cohort 1, importin alpha 1) CDC25C Homo sapiens cell division cycle 25 0.4 0.77 0.43 0.58 homolog C (S. pombe) NUSAP1 Homo sapiens nucleolar and 0.28 0.68 0.22 0.5 spindle associated protein 1 PRC1 Homo sapiens protein regulator of 0.36 0.96 0.38 0.62 cytokinesis 1 FAM64A Homo sapiens family with sequence 0.42 0.59 0.26 0.61 similarity 64, member A LOC651816 PREDICTED: Homo sapiens similar 0.33 0.89 0.31 0.63 to Ubiquitin-conjugating enzyme E2S PLK1 Homo sapiens polo-like kinase 1 0.39 0.71 0.31 0.57 (Drosophila) CDCA3 Homo sapiens cell division cycle 0.28 0.75 0.15 0.49 associated 3 ANLN Homo sapiens anillin, actin binding 0.5 1.03 0.32 0.62 protein PHF19 Homo sapiens PHD finger protein 0.48 0.88 0.29 0.58 19 (PHF19) CEP55 Homo sapiens centrosomal protein 0.39 0.97 0.4 0.63 55kDa CDC2 Homo sapiens cell division cycle 2, 0.31 0.77 0.34 0.65 G1 to S and G2 to M DLGAP5 Homo sapiens discs, large 0.26 0.65 0.2 0.44 (Drosophila) homolog-associated protein 5 NCAPG Homo sapiens non-SMC condensin 0.29 0.76 0.26 0.51 I complex, subunit G CKAP2L Homo sapiens cytoskeleton 0.37 0.83 0.24 0.74 associated protein 2-like KIF15 Homo sapiens kinesin family 0.48 0.79 0.48 0.54 member 15 FEN1 Homo sapiens flap structure- 0.44 1.11 0.46 0.73 specific endonuclease 1 MXD4 Homo sapiens MAX dimerization 2.33 1.43 4 3.36 protein 4 DONSON Homo sapiens downstream 0.43 0.95 0.41 0.56 neighbor of SON C13orf34 Homo sapiens chromosome 13 0.45 0.63 0.4 0.5 open reading frame 34 LOC399942 PREDICTED: Homo sapiens similar 0.47 0.7 0.33 0.52 to Tubulin alpha-2 chain ABCA1 Homo sapiens ATP-binding 2.27 2.61 2.44 2.28 cassette, sub-family A (ABC1), member 1 TMPO Homo sapiens thymopoietin 0.41 0.78 0.36 0.6 (TMPO), transcript variant 1

RRM1 Homo sapiens ribonucleotide 0.45 0.95 0.44 0.7 reductase M1 polypeptide

CCNF Homo sapiens cyclin F (CCNF) 0.28 0.66 0.22 0.47 RDH5 Homo sapiens retinol 2.17 1.1 2.38 1.96 dehydrogenase 5 (11-cis/9-cis) POLE2 Homo sapiens polymerase (DNA 0.43 0.77 0.39 0.49 directed), epsilon 2 (p59 subunit) NCAPD2 Homo sapiens non-SMC condensin 0.3 0.76 0.29 0.62 I complex, subunit D2 E2F2 Homo sapiens E2F transcription 0.35 0.84 0.43 0.72 factor 2 FAM111A Homo sapiens family with sequence 0.47 0.93 0.49 1.05 similarity 111, member A TUBB2C Homo sapiens tubulin, beta 2C 0.38 0.78 0.48 0.83 (TUBB2C) HMMR Homo sapiens hyaluronan- 0.29 0.69 0.22 0.49 mediated motility receptor

FAM83D Homo sapiens family with sequence 0.31 0.73 0.25 0.53 similarity 83, member D PRR11 Homo sapiens proline rich 11 0.42 0.85 0.34 0.68 (PRR11) LOC731314 PREDICTED: Homo sapiens similar 0.38 0.77 0.29 0.64 to H2A histone family, member X

UHRF1 Homo sapiens ubiquitin-like with 0.47 0.95 0.43 0.64 PHD and ring finger domains 1 CCNA2 Homo sapiens cyclin A2 (CCNA2) 0.22 0.63 0.23 0.46 TTK Homo sapiens TTK protein kinase 0.41 0.8 0.42 0.59 (TTK) KIF11 Homo sapiens kinesin family 0.47 0.68 0.34 0.58 member 11 TPX2 Homo sapiens TPX2, microtubule- 0.33 0.8 0.28 0.68 associated, homolog (Xenopus laevis) KIF4A Homo sapiens kinesin family 0.37 0.79 0.29 0.67 member 4A CENPA Homo sapiens centromere protein 0.25 0.65 0.17 0.5 A (CENPA), transcript variant 2 CCNB2 Homo sapiens cyclin B2 0.35 0.7 0.33 0.47 SLC16A4 Homo sapiens solute carrier family 2.7 1.54 2.44 2.14 16, member 4 TK1 Homo sapiens thymidine kinase 1, 0.36 0.97 0.37 0.56 soluble TYMS Homo sapiens thymidylate 0.29 0.74 0.27 0.69 synthetase KIF14 Homo sapiens kinesin family 0.4 0.73 0.31 0.56 member 14 LOC731049 PREDICTED: Homo sapiens similar 0.4 0.93 0.32 0.61 to Ubiquitin-conjugating enzyme E2S GINS2 Homo sapiens GINS complex 0.29 0.74 0.28 0.6 subunit 2 (Psf2 homolog) KIF23 Homo sapiens kinesin family 0.36 0.86 0.31 0.73 member 23, transcript variant 2

SPC25 Homo sapiens SPC25, NDC80 0.43 0.84 0.24 0.43 kinetochore complex component PTTG1 Homo sapiens pituitary tumor- 0.26 0.73 0.26 0.6 transforming 1 PTTG3P Homo sapiens pituitary tumor- 0.3 0.68 0.25 0.5 transforming 3 (pseudogene) (PTTG3P) NEK2 Homo sapiens NIMA (never in 0.33 0.68 0.29 0.53 mitosis gene a)-related kinase 2 NCAPG2 Homo sapiens non-SMC condensin 0.5 0.95 0.4 0.64 II complex, subunit G2 BARD1 Homo sapiens BRCA1 associated 0.45 0.79 0.44 0.66 RING domain 1 HIST1H4C Homo sapiens histone cluster 1, 0.44 0.8 0.39 0.68 H4c RACGAP1 Homo sapiens Rac GTPase 0.4 0.86 0.36 0.63 activating protein 1 DEPDC1B Homo sapiens DEP domain 0.45 0.65 0.45 0.66 containing 1B LMNB1 Homo sapiens lamin B1 0.27 0.62 0.24 0.47 GPSM2 Homo sapiens G-protein signalling 0.44 0.63 0.33 0.48 modulator 2 (AGS3-like, C.elegans) C15orf23 Homo sapiens chromosome 15 0.47 0.91 0.39 0.54 open reading frame 23 (C15orf23)

SPC24 Homo sapiens SPC24, NDC80 0.48 0.67 0.3 0.45 kinetochore complex component OIP5 Homo sapiens Opa interacting 0.49 0.72 0.45 0.51 protein 5 BUB1 Homo sapiens BUB1 budding 0.38 0.67 0.36 0.52 uninhibited by benzimidazoles 1 homolog (yeast) PRIM1 Homo sapiens primase, DNA, 0.4 0.82 0.32 0.49 polypeptide 1 (49kDa) MELK Homo sapiens maternal embryonic 0.43 0.76 0.44 0.52 leucine zipper kinase KIFC1 Homo sapiens kinesin family 0.24 0.7 0.25 0.63 member C1 SMC4 Homo sapiens structural 0.49 0.66 0.36 0.63 maintenance of chromosomes, transcript variant KIAA0101 Homo sapiens KIAA0101, transcript 0.25 0.67 0.26 0.59 variant 1 RPS29 Homo sapiens ribosomal protein 2.13 1.41 2.27 1.53 S29, transcript variant 2, mRNA. FOXM1 Homo sapiens forkhead box M1, 0.38 0.76 0.37 0.62 transcript variant 2 BIRC5 Homo sapiens baculoviral IAP 0.32 0.95 0.26 0.92 repeat-containing 5, transcript

variant 1, INCENP Homo sapiens inner centromere 0.48 0.72 0.37 0.56 protein antigens 135/155kDa,

transcript v AURKA Homo sapiens aurora kinase A, 0.37 0.87 0.33 0.56 transcript variant 3 ZWINT Homo sapiens ZW10 interactor, 0.44 0.79 0.31 0.52 transcript variant 3 EZH2 Homo sapiens enhancer of zeste 0.4 0.75 0.41 0.68 homolog 2 (Drosophila), transcript variant CENPM Homo sapiens centromere protein 0.4 0.8 0.32 0.47 M, transcript variant 2 CCNE2 Homo sapiens cyclin E2, transcript 0.32 0.89 0.49 0.92 variant 2 STIL Homo sapiens SCL/TAL1 0.48 0.85 0.5 0.71 interrupting locus, transcript variant 2 MCM10 Homo sapiens minichromosome 0.46 1.01 0.49 0.77 maintenance complex component 10 LOC10012800 PREDICTED: Homo sapiens 0.5 0.83 0.45 0.7 7 misc_RNA PREDICTED: Homo sapiens 0.44 0.82 0.45 0.7 LOC729816 misc_RNA