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( 12 ) United States Patent US010589003B2 (12 ) United States Patent ( 10 ) Patent No.: US 10,589,003 B2 Raad (45 ) Date of Patent : Mar. 17 , 2020 ( 54 ) METHODS FOR COATING SURFACES WITH (58 ) Field of Classification Search ANTIMICROBIAL AGENTS CPC A61L 29/16 See application file for complete search history . (71 ) Applicant: The Board of Regents of the University of Texas System , Austin , ( 56 ) References Cited TX (US ) U.S. PATENT DOCUMENTS (72 ) Inventor: Issam Raad , Missouri City, TX (US ) 3,635,652 A 1/1972 Streck (73 ) Assignee : The Board of Regents of the 4,015,937 A 4/1977 Miyamoto et al . University of Texas System , Austin , 4,107,121 A 8/1978 Stoy TX (US ) (Continued ) ( * ) Notice: Subject to any disclaimer , the term of this FOREIGN PATENT DOCUMENTS patent is extended or adjusted under 35 EP 0300961 1/1989 U.S.C. 154 (b ) by 0 days . EP 0348947 6/1989 (Continued ) ( 21) Appl. No .: 15 /920,698 ( 22 ) Filed : Mar. 14 , 2018 OTHER PUBLICATIONS (65 ) Prior Publication Data U.S. Appl. No. 08 / 150,472 , Sherertz . (Continued ) US 2018/0303977 A1 Oct. 25 , 2018 Primary Examiner Dah - Wei D. Yuan Related U.S. Application Data Assistant Examiner - Andrew J Bowman (63 ) Continuation of application No. 11/ 560,300 , filed on (74 ) Attorney, Agent, or Firm - Parker Highlander PLLC Nov. 15 , 2006 , now abandoned . (60 ) Provisional application No. 60 / 738,198 , filed on Nov. ( 57 ) ABSTRACT 18 , 2005 . Disclosed are methods for coating or impregnating a surface with an antimicrobial agent that involve contacting the (51 ) Int. Ci. surface with a composition that includes an antimicrobial AGIL 29/16 ( 2006.01 ) agent and a solvent, and curing the surface by applying heat . AOIN 25/24 ( 2006.01 ) Also disclosed are methods for reducing the risk of devel AOIN 25/34 ( 2006.01 ) opment or progression of an infection in a subject in need of A61L 27/54 ( 2006.01 ) a medical device , that involve coating or impregnating a A61L 31/16 ( 2006.01 ) surface of the medical device with an antimicrobial agent (52 ) U.S. CI. and then curing the surface by applying heat, wherein the CPC A61L 29/16 ( 2013.01) ; AOIN 25/24 risk of development or progression of an infection is ( 2013.01 ) ; AOIN 25/34 ( 2013.01) ; A61L 27/54 reduced . ( 2013.01) ; A61L 31/16 ( 2013.01 ) ; AGIL 2300/406 (2013.01 ) ; A61L 2300/442 (2013.01 ) 18 Claims, 18 Drawing Sheets Durability of Spectrum compared to Heated Mino Rifampin catheters over 12 weeks - tested against MRSA 4798 35 30 25 ZoneofInhibition(mm) 20 15 10 5 0 5 10 15 20 25 30 35 40 45 50 55 60 65 70 75 80 85 Days Silicone Spectrum Silicone - MR1 US 10,589,003 B2 Page 2 ( 56 ) References Cited WO WO 2000/007574 2/2000 WO WO 2000/065915 11/2000 U.S. PATENT DOCUMENTS WO WO 2001/054661 8/2001 WO WO 2002/082907 10/2002 4,233,263 A 11/1980 Schaeffer WO WO 2007/024974 3/2007 4,349,029 A 9/1982 Mott 4,442,133 A 4/1984 Greco et al . OTHER PUBLICATIONS 4,592,920 A 6/1986 Murtfeldt 4,863,445 A 9/1989 Mayhan et al. 4,895,566 A 1/1990 Lee U.S. Appl. No. 10 /044,836 , Raad . 4,917,686 A 4/1990 Bayston “ Biofilm : disinfecting biofilms using hydrogen peroxide/ silver based 4,952,419 A 8/1990 Bayston et al. biocide , ” Accepta : Leading eChemical Procurement, http : // accepta . 5,013,306 A 5/1991 Solomon et al . com , 2004 . 5,236,355 A * 8/1993 Brizzolara A61C 19/063 “ Removal of residual microbicide from sterilised medical devices 433/80 5,308,611 A 5/1994 Thompson using a neutralising sol. comprising, e.g. ascorbic acid or an 5,310,524 A 5/1994 Campbell et al . enzyme, ” Dialog File , Derwent WPI , Thompson Derwent , 2004 . 5,344,652 A 9/1994 Hall, II et al . “ Synercid I.V. — Dosing, ” website found at http ://www.synercid . 5,362,754 A 11/1994 Raad et al . com /dosing /default.htm . , Printed Aug. 11 , 2000 . 5,589,507 A 12/1996 Hall, II " Synercid ( R ) Approved in UK , ” Company News on Call. Article 5,616,119 A 4/1997 Davis found at http://www.prnewswire.com/cgi-bin/stories.pl?ACCT+105 5,624,704 A * 4/1997 Darouiche A61L 27/54 & STORY + /www / sto ... 000099630 . Dated Sep. 5 , 2000 . 427 / 2.24 “ Synercid , a new antibiotic , approved in the European Union ; first 5,688,516 A 11/1997 Raad 5,709,672 A 1/1998 Illner Avenits Pharma product approval, ” Company News on Call. Article 5,756,145 A 5/1998 Darouiche found at http://www.prnewswire.com/cgi-bin/stories.pl?ACCT+105 5,811,471 A 9/1998 Shanbrom & STORY + www / sto ... / 000110315. Printed Sep. 5 , 2000 . 5,820,607 A 10/1998 Tcholakiran et al . “ Synercid , Compassionate Use Antibiotic ,” webstie found at www . 5,820,918 A 10/1998 Ronan et al. cystic-1.org/handbook/html/synercid_compassionate_use_an.htm . 5,840,343 A 11/1998 Hall , II et al. Printed Sep. 5 , 2000 . 5,853,745 A 12/1998 Dariouche 5,871,692 A 2/1999 Haire et al. “ The breakthrough technology behind STERRAD sterilization sys 5,902,283 A 5/1999 Darouiche et al. tems, ” Advanced Sterilization Products , Johnson & Johnson Com 5,928,916 A 7/1999 Keogh pany, http://www.sterrad.com . 6,068,972 A 5/2000 Levy “ US FDA Approval of Synercid ( quinupristin /dalfopristin ) I.V., ” 4th 6,162,487 A 12/2000 Darouiche Scientia Europaea Forum News Report, Sep. 20 , 1999. Found at 6,165,484 A 12/2000 Raad et al . http://www.rhone-poulenc.com/bodyu/nw990052.htm . Printed Sep. 6,187,768 B1 2/2001 Wele et al. 5 , 2000 . 6,193,994 B1 2/2001 Lee et al. 6,261,457 B1 7/2001 Wenthold et al. Aeschlimann , et al ., “ Treatment of vancomycin - resistant Enterococ 6,267,979 B1 7/2001 Raad cus faecium with RP 59500 ( quinupristin -dalfopristin ) administered 6,350,251 B1 2/2002 Prosl et al . by intermittent or continuous infusion , alone or in combination with 6,361,524 B1 3/2002 Odell et al . doxycycline , in an in vitro pharmocodynamic infection model with 6,368,317 B2 4/2002 Chang simulated endocardial vegetations ,” Antimicrob . Agents Chemother. , 6,428,799 B1 8/2002 Cen et al. 42 : 2710-2717 , 1998 . 6,448,006 B1 9/2002 Levy 6,503,539 B2 1/2003 Gestrelius et al. Aumercier, et al. , “ P59500 .: A proposed mechanism for its bacte 6,509,319 B1 1/2003 Raad et al . ricidal activity ,” J. Antimicrob . Chemother ., 30 ( Suppl. A ) : 9-14 , 6,585,934 B1 7/2003 Oberleitner et al . 1992 . 6,592,564 B2 7/2003 Finch Bergeron and Montay , “ The pharmacokinetics of quinupristin / 6,679,870 B1 1/2004 Finch et al . dalfopristin in laboratory animals and in humans, ” J. Antimicrob . 6,685,694 B2 2/2004 Finch et al. Chemoter. , 39( Suppl . A ) : 129-138 , 1997 . 2001/0047195 A1 11/2001 Crossley et al . Bhatnager and Sundaram , “ Studies on antibacterial properties of 2002/0007175 A1 1/2002 Chang gentian violet impregnated silastic , ” Indian J.Med . Res. , [ A ]97 :206 2002/0052404 A1 5/2002 Hunter 208, 1993 . 2002/0087156 A1 7/2002 Maguire et al . Blot, et al. , “ Diagnosis of catheter- related bacteremia : a prospective 2002/0133072 A1 9/2002 Wang comparison of the time to positivity of hub -blood versus peripheral 2003/0007939 A1 1/2003 Murad blood cultures, " Lancet , 354 : 1071-1077 , 1999 . 2003/0032605 A1 2/2003 Raad et al. Chaiban et al. , “ A rapid method of impregnating endotracheal tubes 2003/0065292 Al 4/2003 Darouiche and urinary catheters with gendine : a novel antiseptic agent, ” 2003/0078242 Al 4/2003 Raad et al . Journal of Antimicrobial Chemotherapy 55 :51-56 , 2005. 2004/0254545 Al 12/2004 Rider, II et al . Chatzinikolaou et al. , “ Minocycline and Edta ( M - EDTA ) as a flush 2005/0013836 A1 1/2005 Raad solution for implantable ports ( IP ) used in pediatric cancer patients, " 2005/0197634 Al 9/2005 Raad et al . Shea Merck Healthcare Epidemiology Search Abstracts , 2002 . Curbelo , et al ., “ Treatment and outcome in 100 patients with FOREIGN PATENT DOCUMENTS vancomycin - resistant enterococcal (VRE ) bacteremia ,” Abstract J - 7 . In Program and abstracts of the 37th Interscience Conference on EP 0328421 8/1989 Antimicrobial Agents and Chemotherapy , Washington , DC ., 1997. EP 0865785 9/1998 Desautels et al. , “ Maintenance of sterility in urinary drainage bags ,>>” EP 1044686 10/2000 EP 1245247 10/2002 Surg . Gynecol. Obstet. , 154 ( 6 ) : 838-840 , 1982 . GB 2007096 5/1979 Dever , et al. , “ Treatment of vancomycin - resistant Enterococcus WO WO 1994/010838 5/1994 faecium infections with an investigational streptogramin antibiotic WO WO 1995/005203 2/1995 ( quinupristin /dalfopristin ) : A report of fifteen cases, ” Microb . Drug WO WO 1995/032625 12/1995 Resist. , 2 : 407-413 , 1996 . WO WO 1997/018707 5/1997 Donelli et al, “ Pharmacokinetics of anticancer agents in patients WO WO 1999/017791 4/1999 with impaired liver function ,” Abstract, European Journal of Can WO WO 2000/001238 1/2000 cer, Pergamon Press, UK , 34 ( 1 ) :33-46 , 1998 . US 10,589,003 B2 Page 3 ( 56 ) References Cited Moellering, Jr., “ Vancomycin - resistant Enterococci, " Clinical Infec tious Diseases , 26 : 1196-1199 , 1998 . OTHER PUBLICATIONS Montecalvo , et al. , “ Bloodstream infections with vancomycin resistant Enterococci, ” Arch . Intern . Med . , 156 : 1458-1462, 1996 . Edmond , et al. , “ Vancomycin -resistant enterococcal bacteremia : Moreno , et al ., 1994. “ An old antibiotic for a new multiple -resistant Natural history and attributable mortality ,” Clin .
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