molecules Article Proteome Investigation of Rat Lungs Subjected to Ex Vivo Perfusion (EVLP) Valentina Roffia 1,2,† , Antonella De Palma 2,† , Caterina Lonati 3 , Dario Di Silvestre 2 , Rossana Rossi 2 , Marco Mantero 4, Stefano Gatti 1, Daniele Dondossola 5, Franco Valenza 6, Pierluigi Mauri 2,* and Francesco Blasi 4 1 Center for Preclinical Research, Fondazione IRCCS Ca’ Granda-Ospedale Maggiore Policlinico, 20122 Milan, Italy; valentina.roffi
[email protected] (V.R.);
[email protected] (S.G.) 2 Proteomics and Metabolomics Unit, Institute for Biomedical Technologies (ITB-CNR), 20090 Segrate (MI), Italy;
[email protected] (A.D.P.);
[email protected] (D.D.S.);
[email protected] (R.R.) 3 Department of Anesthesia and Critical Care, Fondazione IRCCS Ca’ Granda Ospedale Maggiore Policlinico, 20122 Milan, Italy;
[email protected] 4 Department of Pathophysiology and Transplantation, Università degli Studi di Milano, and Internal Medicine Department, Respiratory Unit and Adult Cystic Fibrosis Center, Fondazione IRCCS Cà Granda Ospedale Maggiore Policlinico, 20122 Milano, Italy;
[email protected] (M.M.);
[email protected] (F.B.) 5 Surgery and Liver Transplant Center, Fondazione IRCCS Ca’ Granda-Ospedale Maggiore Policlinico, 20122 Milan, Italy;
[email protected] 6 Department of Pathophysiology and Transplantation, University of Milan, 20122 Milan, Italy;
[email protected] * Correspondence:
[email protected]; Tel.: +39-02-2642-2736 † These authors contributed equally to this work. Received: 15 September 2018; Accepted: 21 November 2018; Published: 22 November 2018 Abstract: Ex vivo lung perfusion (EVLP) is an emerging procedure that allows organ preservation, assessment and reconditioning, increasing the number of marginal donor lungs for transplantation.