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@M Table of Contents Acknowledgements ......................................................................................................................... iv List of Figures ................................................................................................................................ vi List of Tables ................................................................................................................................. vii List of Abbreviations .................................................................................................................... viii CHAPTER 1 .................................................................................................................................... 1 Literature Review ............................................................................................................................ 1 Lyme Disease Epidemiology ........................................................................................................... 1 The Enzootic Cycle of Borrelia burgdorferi ................................................................................... 2 Borrelia burgdorferi Physiology ..................................................................................................... 3 Lyme Disease Pathogenesis ............................................................................................................ 5 The innate immune response to Borrelia burgdorferi .................................................................... 6 The adaptive immune response to Borrelia burgdorferi ............................................................... 10 Borrelia burgdorferi evasion and dissemination .......................................................................... 11 References ..................................................................................................................................... 14 CHAPTER II ................................................................................................................................. 22 Insights in the Characterization of the Immune Response to B. burgdorferi Peptidoglycan ....... 22 Introduction ................................................................................................................................... 22 Methodology ................................................................................................................................. 23 Results ........................................................................................................................................... 27 Discussion ..................................................................................................................................... 32 References ..................................................................................................................................... 67 CHAPTER III ................................................................................................................................ 71 NapA is a Peptidoglycan Associating Protein in B. burgdorferi .................................................. 71 Introduction ................................................................................................................................... 71 Methodology ................................................................................................................................. 72 Results ........................................................................................................................................... 80 Discussion ..................................................................................................................................... 88 References ................................................................................................................................... 108 CHAPTER IV .............................................................................................................................. 114 Conclusions and Future Directions ............................................................................................ 114 References ................................................................................................................................... 118 M -562*-+74)5 @>LI< *!JKIL:KLI<8E;;@==<I<EK@8K@FE @>LI<FOGI<JJJ@FEE8CPJ@J @>LI<!)EI@:?D<EKE8CPJ@J,<JLCKJ=FIK?<*'J-K@DLC8K<;N@K?8:K<I@8C*!J=FI 8E;"FLIJ @>LI<!<E<OGI<JJ@FEE8CPJ@J@E*'J-K@DLC8K<;N@K?8:K<I@8C*!J=FI 8E; 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