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Prof.Dr. Nermin GÖZÜKIRMIZI

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Prof.Dr. Nermin GÖZÜKIRMIZI Kişisel Bilgiler İş Telefonu: +90 212 455 5700 Dahili: 1515101 EF-apxo Tsetale: fnoenrum:i n+@9i0s t2a1n2b u4l5.e5d u5.8tr11 PWoesbta: hAtdtpr:e//sai:v İe.Üs..iFsteann Fbaukl.üedltues.tir, /Mnoelremküinle/r Biyoloji ve Genetik Bölümü, 34134 Vezneciler-İstanbul Biyografi Prof.Dr.Nermin Gözükırmızı, İstanbul Üniversitesi Profesörü, İzmit, Türkiye'de 18 Haziran 1951 tarihinde doğdu. A19n6ab7i lyimılın Ddaal ıg'nirddai ğPi rİostfa. Dnbr.u El mÜninivee Brsiligtes’ni iBno dtaonkitko-rZao öoğlorjei nBcöislüi omlaürnadke bna 1ş9la7d2ığ yı ıdlınodktao mrae çzaulnış omldalua r vıneı P rBooft.Danri.Nk evbea Gheantetik ıYşaınklaarr-ıTna ent kyiölenrei’tdimiri. nde 1979 yılında tamamladı. Doktora tezi başlığı: Vicia faba üzerine bazı kimyasal maddelerin ve 1 985 İstanbul Üniversitesi Biyoloji Bölümü'e doçent olarak atandı ve 2003 yılından itibaren akademik çalışmalarını Moleküler Biyoloji ve Genetik Bölümünde sürdürmektedir ve 1993 yılında profesör ünvanını almıştır. 1N9e9rm2-i2n0 G0ö6z Büiktıkrim Bıizyıo TtÜekBnİToAloKji, A Mraşrtmıramra BAirraimştıirnmina vMee İrsktaenzib, uGle Ünn Mivüehresintedsisi lMiğoi vleek Bülieyro tBeikynoololojij iv Ae rGaeşntıermtika BEönlsütmitülesrüi,nin P koulrimulomrafiszımnd vae aMkotilfe gköürleerv malamrkışetrırla. Ar,r GaDştOır manaa alilzain vlea rbı;i yGoe-ngeütvike,n Bliikt kvie d Eopkiug eknüelttüikr üo lsairsatekm talenrıim vlea nGaebni ltir.a nLsisfearnis, ve lisansüstü düzeyinde dersler vermektedir. bAirlipmas, eblu mğdaakya,l ep a(t7a0te) sv, en okihtaupt, bkaövlüamk, lperaimniunk ,( p2i0ri)n yça gziabri ıfdaırk. Slıo bnit ykıil atürrdlaekri iulel ubsilaimrasreal sçıa ylıaşymınallanrımnıınş byialinmıssıeral ,m açkoakl eslaeyrıida Öreğtrreontrcailnersip (oYzaorndla.Dro, gçe., nD torça.,nPsrfoefr.)i vTeü srtkrieyse tvoel edriağnesrı üillek eillgeirliddeir ö. n2e0m ylüi kbsielikm lsiseal nksa tvkeıl a2r0 s daoğklatmoraak teazdiı rylöanr.etmiştir. Eğitim Bilgileri Post Doktora, TUhneiv Uernsiviter Dsi't'y A Omf iHenosu, sBtoionl,o Bgiyo Dloegpya Drtempeanrtm, Fernatn, sAam 1e9r8ik5a -B 1ir9l8e5şik Devletleri 1979 - 1980 LDiosakntosr, aİs, tİasntabnubl uÜl nÜivneivresritseistei,s Fi,e Fne Fna Fkaükltüelstei,s Bi, oDtar.nRiekr-Z.Noaotl,o Tjiü, rTküiyrkei y1e9 7159 6- 81 9- 719972 Yabancı Diller Yİnagbiliazcnec, Bı 2D Oilrltea rÜstü Sertifika, Kurs ve Eğitimler IDDiğBe arn, Tdh TeU IBnItTerAnKa-tMioRnCa-lR TIhGeEoBr,i t2ic0a0l1 Course on genetics and Molecular Biology of Stress Tolerance in Plants, COMSTECH, Eğitim Yönetimi ve Planlama, ARpFpLlPic iant iPolnasn to Bf creleludlianrg ,a InCdG EMBo, l1ec9u9l1ar Techniques to Plant Germplasm Conservation, ICRO- EUğNitEimSC YOö-UnNetIiLm, i1 v9e9 0Planlama, Recent Developments in Biotechnology, The Instıtute for Biotechnological studies, 1985 Yaptığı Tezler 1D9o7k9tora, Bazı Kimyasal Madde ve Işınların Vicia faba L. Üzerine Etkileri, İstanbul Üniversitesi, Botanik-Genetik, Genetik, Araştırma Alanları MYaüşhaemn dBiislilmiğil,e Grie, nBoiymoitkesk, nToelmojei,l BBiitlkimi Bleiyroteknolojisi, Moleküler Biyoloji ve Genetik , Bitki Moleküler Genetiği, Genetik APrkoaf.Ddre.,m İsitakn Ubunl vÜaninvelarsri t/es Gi, Föerne Fvalkeürltesi, Moleküler Biyoloji ve Genetik Bölümü, 1993 - Devam Ediyor Aİstkaandbuelm Ünikiv eİrdsiatersii ,D Meonleekyüliemr Biyoloji Ve Genetik, 2005 - 2009 VBietkrid Dioğkiu D Keürltsülreür, Lisans, 2014 - 2015, 2015 - 2016, 2016 - 2017, 2017 - 2018 TKermomelo Gzoenme tBikiy, oLliosajinsis, ,L 2is0a1n3s ,- 22001134 ,- 22001144 ,- 22001145 ,- 22001165 ,- 22001167 ,- 22001177 - 2018 BİnİsTaKnİ GMeOnLeEtiKğÜi, LEisRa nBsİ,Y 2O0L1O6J İ-S 2İ 0V1E7 G, 2E0N1E7T İ-Ğ 2İ0 L1A8BORATUAR PROJESİ, Yüksek Lisans, 2017 - 2018 İBşiltekvi sMelo gleeknüolmeri kB, iDyolkotjoisria v, e2 0G1e4n e- t2iğ0i1 L5a, b2o0r1a5tu -a 2r0 P1r6o,j e2s0i1, Y6ü -k 2s0ek1 7Lisans, 2015 - 2016, 2016 - 2017 İDşolekvtsoerla g Aenlaonm Dike,r Dsio, Dktookrtao, r2a0, 1230 1- 72 0- 1240,1 28017 - 2018 BKirtokmi Moozolemkü Bleiyro Bloiyjioslios,j iLsiis, aLnissa, n2s0, 1250 1- 62 0- 126017 Bitikrimleere P Groejne sTir, aLnissafners , Y2ö0n1t4e m- 2le0r1i, 5Yüksek Lisans, 2016 - 2017 BSeitmkii nMeorl, eLkiüsalenrs ,B 2iy0o1l3o j-i s2i 0v1e4 G, e2n0e1t4iğ -i L2a0b1o5ratuar Projesi, Yüksek Lisans, 2014 - 2015 Doktora Alan Dersi, Doktora, 2014 - 2015, 2015 - 2016 Bİnitskain M Goelnekeütilğeir, LBisiyaonlso, j2is0i 1v4e G- 2en0e1t5iğ, i2 L0a1b5o -r 2at0u1a6r Projesi, Yüksek Lisans, 2013 - 2014 Bitkilered eG Rene kTormanbsinfearn Yt öPnrotetmeinle rÜir, eYtüimksie vke L İizsoalnas,y 2o0n1u4, D - o2k0t1o5ra, 2014 - 2015 İBNİTSAKNİ M GOELNEEKTÜİĞLİE, RL iBsaİYnOs,L 2O0J0İS9İ -V 2E0 G1E0N, 2E0T1İĞ0İ - L 2A0B1O1R, 2A0T1U3A -R 2 P0R1O4JESİ, Yüksek Lisans, 2011 - 2012, 2012 - 2013 BİTKİL DEORKDUE KRÜELKTOÜMRBÜİ,N LAisNaTn sP, R2O0T0E9İ -N 2 Ü0R1E0T, 2İM01İ 0V E- 2İZ0O1L1A, 2S0Y1O3N -U 2, D01o4ktora, 2009 - 2010, 2010 - 2011, 2011 - 2012, 2013 İ-Ş 2L0E1V4SEL GENOMİK, Doktora, 2012 - 2013 BKİRTOKMİ MOZOOLMEK BÜİLYEORL OBJİYSOİ, LLOisJaİSnİs, ,L 2is0a1n1s ,- 22000192 ,- 22001120 ,- 22001103 - 2011, 2013 - 2014 GBEitLirİmŞİeM P GroEjNesEiT, LİĞisİa, Lniss, a2n0s0, 82 0- 1210 0- 92,0 21020, 92 0- 1220 1- 02,0 210310 - 2011, 2011 - 2012, 2012 - 2013 TBEİTMKEİLE GREEN GEETNİK T, LRiAsaNnSsF, E2R0 1Y1Ö N- 2T0E1M2L, E2R0İ1, 2Y ü- k2s0e1k3 Lisans, 2009 - 2010, 2010 - 2011, 2013 - 2014 DBİETVKEİL MOOPLMEEKNÜTLAELR G BEİNYOELTOICJSİS, İL, iLsiasnasn, s2, 02081 2- 2- 02091,3 2009 - 2010, 2010 - 2011, 2012 - 2013 BİTKİ LDEORKDUE KRÜELKTOÜMRBÜİ,N LAisNaTn sP, R2O01T1E İ-N 2 Ü0R12E,T 2İM01İ 2V E- 2İZ0O13LASYONU, Doktora, 2011 - 2012, 2012 - 2013 BGİETNKEİL TERRAEN GSEFNER TSR TAON SPFLEARN TYSÖ N(ETREAMSLMEURSİ,) Y, Yüükskeske kL Lisiasnans,s 2, 2001122 - -2 2001133 BİNİTSKAİN M GOELNEEKTÜİĞLİE, RL iBsaİYnOs,L 2O0J1İS1İ , -L 2is0a1n2s,, 22001121 -- 22001132 BİŞİLTEKVİ SMEOLL GEEKNÜOLMERİK B, İDYoOkLtOorJİaS, İ2 V0E0 8G E- N20E0T9İĞ, İ2 L0A09B O- R2A01T0U,A 2R0 1P0R O- J2E0S1İ,1 Yüksek Lisans, 2008 - 2009, 2009 - 2010, 2010 - T2E0M11EL GENETİK, Lisans, 2010 - 2011 KROMOZOM BİYOLOJİSİ, Lisans, 20089 - 200190, 2010 - 2011 Yönetilen Tezler EG.ÖKZaÜrlKıkI(RÖMğIrZeIn Nci.), ,A 2r0p1a8 (Hordeum vulgare L. )’da Uzun Kodlama Yapmayan RNA’lar Üzerine Çalışmalar, Doktora, 2G0Ö1Z8ÜKIRMIZI N., İnsan Genomunda Retrotranspozon ve Endojen Retrovirüs Analizleri, Doktora, B.Çakmak(Öğrenci), HGÖOZMÜOKBIRAMSISZİIN NO.,S TTUEZR OSTİDR EESTİK AİSLİT, IDNoDkAto YraE,T Sİ.ŞMTaİRraİLkElı(NÖ AğrRePnAc i()H, 2o0r1d6eum vulgare L.) BİTKİLERİNE GÖZÜKIRMIZI N., BPiarkinırç 'Stter e(Osir vyez aB sraatsisvian oLs.)t eGrDoOid lveer itnr aAnysçpiçoesğoin ( Hanealilaiznltehrui, sD aonkutoursa L, .G) .üYzüezrbianşeı oeğtkluil(eÖriğ, rYeünkcsi)e,k 2 L0i1sa6ns, GKÖ.BZeÜtüKlI(RÖMğrIeZnI cNi).,, A2r0p1a4'da (Hordeum vulgare L.) endojen sirevirus analizi, Yüksek Lisans, B.Çakmak(Öğrenci), 2014 KG.ÖŞZahÜiKnI(RÖMğrIZenI cNi)., , İ2n0sa1n4da SIRT6 geninde Yaşlanmaya Bağlı Meydana Gelen Genetik ve Epigenetik Değişiklikler, Doktora, DGÖokZtÜoKraIR, GM.KIZAI RNT.,A ALr(pÖağ (r eHnocri)d, e2u0m13 vulgare L. ) Doku kültüründe Sukkula retrotranspozon hareketlerinin belirlenmesi, tGhÖrZoÜugKhIR AMgIrZoIb Nac., tGereinuemra –t imone doifa hteedr btricaindsef-otorlmeraatinotn t,o Dboakcctor(aN, iYco.KtAiaYnAa( tÖağbraecnucmi)), 2b0y 1e3xpressing dehalogenase gene GÖZÜKIRMIZI N., Arpa (Hordeum vulgare L.) Doku Kültüründe Retrotranspozon Hareketlerinin ve Ürünlerinin GBÖelZirÜleKnIRmMesIZi, ID No.k, Dtoorkau, S k.Yüılltmüraüzn(Ödğer teunzc (i)N, a2C0l1) 3stresi kopşullarında yetiştirilen arpa ( Hordeum vulgare L. ) bitkilerinde GgeÖnZeÜtiKkI RveM eIZpIig Ne.n, eİntiska ann Eanlidzloejre,n Y Rükestreokv Lirisüasn Hs, (AH.DEERMV-İHR)K'IınR AGNen(Öoğmriekn Acin)a, l2iz0i1, Y2üksek Lisans, M.GULİYEV(Öğrenci), 2012 SG.ÖMZAÜRKAIKRLMII(ZÖIğ Nre., nAcri)p,a 2 (0H1o2rdeum vulgare L.) Kültür Varyetelerinde Transpozon ve Kromozom Analizleri, Yüksek Lisans, LGiÖsaZnÜsK, IER.BMAIZYIR NA.M, A(rÖpğar e(n Hcio)r, d2e0u1m1 vulgare L. ) doku kültürlerinde Nikita retrotranspozonunun hareketleri, Yüksek GÖZÜKIRMIZI N., Anrptiak aDrosiknuo Kgeünltiükr alenrtiinbdiyeo Gtike neeptiirku vbeis Einpiing ein evtiitkr oV abritykai sgyeolinşliamri, Düzoekrtionrea e, Atk.TileErMi, EDLo(kÖtğorrean,ci), 2011 GHÖ.HZAÜMKAIRTM(ÖIZğIr eNn.,c Ai)r, p2a0 (1H1ordeum vulgare L.) Doku Kültürlerinde Transpozon Analizi, Yüksek Lisans, GC.ÖEZVÜRKEINRSMEILZ(IÖ Nğ.r, eTnecki )y, u2m01u0rta ikizlerinde metilasyon analizleri, Yüksek Lisans, K.ŞAHİN(Öğrenci), 2010 2G0Ö0Z8ÜKIRMIZI N., VenözTromboli Hastalarında Faktör VIII Gen Bölgesinin Moleküler Tanısı, Doktora, M.AY(Öğrenci), G.ÖKZAÜRKTIARLM(IÖZğIr Nen., cBi)r,a 2ss0i0n8osteroidlerin arpa (Hordeum vulgare L.) üzerine sitogenetik etkileri, Yüksek Lisans, LGiÖsaZnÜsK, IBR.EMRIZEIN N(Ö., ğArrepnac i()H, 2o0rd0e7um vulgare )bitkisinde bakırın in vitro koşullarda kök gelişimi üzerine etkileri, Yüksek bGuÖğZdÜaKyI RçeMştIlZeIr Nin.d, Yer t1a0ra Bnumğadsaıy, Y (üTkrsiteicku Lmis anest, iAvu.TmEM LE.)L s(aÖrğı rpeansc (i)P, u2c0c0in6ia striiformis) dayanıklılık geninin ekmeklik GÖZÜKIRMIZI N., DKaolkıtus aKl üTlrtüormübnodfeil iYneinti şMtiroilleeknü Alerrp Aa n(aHloizri,d Deuomkt ovrual,g Bar.DeÖ LL.)E ÜKz(eÖrğirneen Bcia)k, ı2r0ın0 (4Cu+2) Etkilerinin GİnÖcZeÜleKnImReMsIiZ”,I İ Nst.a, nPbaulo Üwnniviae resliotensgia, tYaü’nkısne kD Loiksua nKsü, lDtü.ÖrüZ CSAisNte(mÖğlerreinncini) ,O 2p0ti0m3izasyonu ve Sentetik Tohum Üretimi, Doktora, GZ.ÖİPZEÜKKÇIRİ(MÖğIZrIe nNc.,i )P, a2t0at0e2s (Solanum tuberasum L.) Çeşitlerinde Polifenol Oksidaz Genleri Üzerine Çalışmalar, Doktora, GE.ÖAZRÜICKAIRNM(ÖIZğIr eNn.,c Ai)r, p2a0 (0H2ordeum vulgare
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  • Transposons Continue the Amaze

    Transposons Continue the Amaze

    Gozukirmizi, N. International Journal of Science Letters. 2019. 1(1): 1-13. Review Transposons continue the amaze Nermin Gozukirmizi 1Department of Molecular Biology and Genetics, Faculty of Art and Science, Istinye University, Istanbul/Turkey Abstract Article History Transposable elements (TEs) were first discovered in maize plants. Received 01.06.2019 However, they exist almost in all species with a few exceptions Accepted 02.08.2019 (Plasmodium falciparum, Ashbya gossypii and Kluveromuyces lactis). They are the most important contributors to genome plasticity and evolution and even epigenetic genome regulation. Organisms with large genomes have high transposon percentages. For example, Arabidopsis thaliana has a genome size of 125 Mb which comprises 14% Keywords transposons, Homo sapiens (3000 Mb) 45-48.5%, and Hordeum vulgare genome (5300 Mb) has 80%. TEs are classified into two major groups Evolution, based on their transposition mechanisms: Class I (RNA transposons – Genome dynamics, retrotransposons) and Class II (DNA transposons). Recent progress in Mobile elements, whole-genome sequencing and long-read assembly have resulted in Over-sized identification of unprecedentedly long transposable units spanning transposable elements, dozens or even hundreds of kilobases, initially in prokaryotic and more Transposon based recently in eukaryotic systems. All TEs in a cell are named as genome editing transposome (mobilome), and transposomics is a new area to work with transposome. Although a number of bioinformatics softwares have recently been developed for the annotation of TEs in sequenced genomes, there are very few computational tools strictly dedicated to the identification of active TEs using genome-wide approaches. In this review article, after a brief introduction and review of the transposable elements, I discussed their effects in gene expression, evolution, recent applications and also share our research on retrotransposons with different organisms.
  • RETROTRANSPOSON BASED MARKERS and THEIR APPLICATIONS in BARLEY (Hordeum Vulgare L.Cvs.) TISSUE CULTURE

    RETROTRANSPOSON BASED MARKERS and THEIR APPLICATIONS in BARLEY (Hordeum Vulgare L.Cvs.) TISSUE CULTURE

    PROCEEDINGS ______ The 5th International Symposium on Sustainable Development_______ ISSD 2014 RETROTRANSPOSON BASED MARKERS AND THEIR APPLICATIONS IN BARLEY (Hordeum vulgare L.cvs.) TISSUE CULTURE Nermin Gozukirmizi Istanbul University, Department of Molecular Biology and Genetics, 34134 Vezneciler, Istanbul/Turkey E-mail: [email protected] Abstract Barley has economical value and it is an important model plant. Transposons cover more than 80% of barley genome. More than 40 retrotransposons were characterized in barley genome. This type of transposons replicate via RNA and move in the genome. As a result of these movements, mutations and genome enlargements are occurred. During the recent years, active transcripts and protein products of some retrotransposons have been determined. Somaclonal variations are spontaneously occurred variations in tissue culture conditions. These variations could be produced by genetic and/or epigenetic mechanisms and result in problems in gen transfer applications. We investigated the retrotransposon movements in barley tissue culture and regenerated plantlets using inter retrotransposon amplified polymorphism (IRAP), inter primer binding side (iPBS) and analytical techniques (DNA and RNA levels) and determined the relationship between retrotransposon movements, changes in copy number and differention in culture conditions. For these purposes BARE1, NIKITA, BAGY2 and SUKKULA retrotransposons were analyzed. Our research results show that tissue culture conditions and time increase the transposon based variation and copy numbers of retrotransposons and thus, cause genome enlargements. This research will be contribute the understanding of basic mechanisms related to plant development and differentiation in cultured material and also restriction of variations in applications. Keywords: Barley, Tissue Culture, Retrotransposon markers, Somaclonal variation 1 | P a g e ISSD 2014 The 5th International Symposium on Sustainable Development_______ PROCEEDINGS 1.
  • Long Non-Coding Rnas

    Long Non-Coding Rnas

    Journal of Analytical & Pharmaceutical Research New Player of ncRNAs: Long Non-coding RNAs Abstract Mini Review Long non-coding RNAs (lncRNAs) play important roles in a wide range of biological processes as regulatory factors at the epigenetic, transcriptional and Volume 3 Issue 4 - 2016 of lncRNAs discoveries including their identification, classifications and functions. post- transcriptional levels. In this review, we summarized the current knowledge 1Department of Biotechnology, Istanbul University, Turkey Keywords: Long non-coding RNAs; Discovery of lncRNAs; Classification of 2Department of Molecular Biology and Genetics, Istanbul University, Turkey lncRNAs; Post- transcriptional levels; Epigenetic; Regulatory factors *Corresponding author: Nermin Gözükırmızi, Department of MolecularBiology and Genetics, Istanbul University, Faculty of Science, Istanbul, 34118 Vezneciler, Turkey, Email: Received: | Published: November 04, 2016 November 15, 2016 Introduction Novel lncRNAs can be detected and discovered by both The mechanism underlying the functions of non-protein coding RNAs (ncRNAs or npcRNAs) that have no or little protein-coding experimental (next generation sequencing, NGS, technologies) potential is a fascinating area of research [1]. Based on transcript microarrays.and computational Then, thescreenings transcripts [33-35]. sequences First, are the mapped fragments to the of transcripts are obtained by using NGS technologies or tilling The criteria for discriminating between coding and non-coding length, ncRNAs are classified as short (<200 nt) and long ncRNAs as cDNA/EST in silico mining, whole-genome tilling array and sequencesreference genome of RNAs and are identified based on transcribed similarity unitsto known of the codingRNAs. (lncRNAs; >200 nt). The recent high-throughput analysis such RNA-sequencing (RNA-seq) has revealed that the transcription sequences or statistics of codon frequencies for coding potential landscape in eukaryotes is much more complex than had been to[36].
  • 2 2019 Issue / Sayı: 3

    2 2019 Issue / Sayı: 3

    Volume / Cilt: 2 2019 Issue / Sayı: 3 Editor in Chief / Baş Editör Assit. Prof. Dr. Yilmaz Kaya, Department of Agricultural Biotechnology Ondokuz Mayis University, Turkey Editorial Boards/ Editör Kurulu Fahrul Zaman Huyop, Department of Biosciences, Faculty of Science, Universiti Teknologi Malaysia, Malaysia, Ismail Kocaçalışkan, Department of Molecular Biology and Genetics, Yildiz Technical University, Turkey Ercan Bursal, Department of Nursing Science, Muş Alparslan University, Turkey Nermin Gozukirmizi, Department of Molecular Biology and Genetics, Istinye University, Turkey Azzmer A. Abdul Hamid, Department of Biotechnology, International Islamic University Malaysia, Malaysia Muhammet Kurulay, Department of Mathematics Engineering, Yıldız Technical University, Turkey Ertan Ermis, Department of Food Engineering, Istanbul S. Zaim University, Turkey Mohamed Faraj Edbeib, Department of Animal Production, Faculty of Agriculture, Baniwalid University, Libya Ali Yuksek, Department of Islamic Law, Ondokuz Mayıs University, Turkey Hasan Murat Aksoy, Department of Plant Protection, Ondokuz Mayıs University, Turkey Ayşe Feyza Tufan, Department of Molecular Biology and Genetics, Halic University, Turkey Muhammed Yüceer, Department of Food Processing, Çanakkale Onsekiz Mart University, Turkey Sevgi Marakli, Department of Medical Techniques and Services, Amasya University, Turkey Sibel Yilmaz, Department of Molecular Biology and Genetics, Yeni Yüzyıl University, Turkey, Sahin Ozel, Department of Chemistry, Bursa Uludağ University, Turkey Kasım
  • Editor in Chief / Baş Editör Asst. Prof. Dr. Yilmaz Kaya Ondokuz Mayis University, Turkey

    Editor in Chief / Baş Editör Asst. Prof. Dr. Yilmaz Kaya Ondokuz Mayis University, Turkey

    Volume / Cilt: 3 2020 Issue / Sayı: 2 Editor in Chief / Baş Editör Asst. Prof. Dr. Yilmaz Kaya Ondokuz Mayis University, Turkey Section Editors / Bölüm Editörleri* * sıralama akademik unvan içinde alfabetik sıralamaya göredir. * The ranking is arranged alphabetically within the academic title. Prof. Dr. Ali ASLAN, PhD, Van Yuzuncuyıl University Prof. Dr. Ercan BURSAL, PhD, Muş Alparslan University Prof. Dr. Hasan AKAN, PhD, Harran University Prof. Dr. Nermin GOZUKİRMIZİ, PhD Istinye University Prof. Dr. Tengku Haziyamin TENGKU ABDUL HAMİD, PhD, International Islamic University Malaysia Assoc. Prof. Dr. Ayhan HORUZ, PhD Ondokuz Mayıs University Assoc. Prof. Dr. Hasan Murat AKSOY, PhD, Ondokuz Mayıs University Editorial Board / Editör Kurulu Prof. Dr. İsmail KOCAÇALIŞKAN, PhD, Yildiz Technical University Prof. Dr. Muhammet KURULAY, PhD, Yildiz Technical University Assoc. Prof. Dr. Gulbubu KURMANBEKOVA, Kyrgyz-Turkish Manas University Assoc. Prof. Dr. İsmail ERPER, PhD, Ondokuz Mayıs University Assoc. Prof. Dr. Muhammad Arshad JAVED, PhD, Universiti Teknologi Malaysia Assoc. Prof. Dr. Roswanira AB. WAHAB, PhD, Universiti Teknologi Malaysia Assoc. Prof. Dr. Sevgi MARAKLI, PhD, Amasya University Asst. Prof. Dr. Abdussamat GÜZEL, PhD, Inonu University Asst. Prof. Dr. Ali Yuksek, PhD, Ondokuz Mayıs University Asst. Prof. Dr. Cihan İNAN, PhD, Karadeniz Technical University Asst. Prof. Dr. Ertan ERMİŞ, PhD, Istanbul Sabahattin Zaim University Asst. Prof. Dr. Feyza TUFAN, PhD, Halic University Asst. Prof. Dr. Harun ÖZER, PhD, Ondokuz Mayıs University Asst. Prof. Dr. Kasım TAKIM, PhD, Harran University Asst. Prof. Dr. Mohamed EDBEİB, PhD, Baniwalid University, Libya Asst. Prof. Dr. Muhammed YÜCEER, PhD, Canakkale Onsekiz Mart University, Dr. Aliyu ADAMU, PhD, Kaduna State University Dr. Lect. Abdulgani DEVLET, PhD, Bilecik Seyh Edebali University Dr.
  • 211 Nikita Retrotransposon Movements in Callus Cultures of Barley (Hordeum Vulgare

    211 Nikita Retrotransposon Movements in Callus Cultures of Barley (Hordeum Vulgare

    POJ 5(3):211-215 (2012) ISSN:1836-3644 Nikita retrotransposon movements in callus cultures of barley (Hordeum vulgare L.) Emre Bayram, Sibel Yilmaz, Halide Hamat-Mecbur, Gonul Kartal-Alacam, Nermin Gozukirmizi* Istanbul University, Faculty of Science, Department of Molecular Biology and Genetics, 34134, Vezneciler, Istanbul-TURKEY *Corresponding author: [email protected] Abstract Retrotransposons are dynamic elements of the genome and exist in high percentages in the genome of many organisms. However, the majority of retrotransposons are inactivated during development by different mechanisms such as methylation. Some stress conditions may have a stimulating effect on the activation of retrotransposons. In vitro culture conditions can be considered as one of these stress factors due to nutrients, chemicals, physical factors and photoperiods. In this study, Nikita retrotransposon polymorphism was investigated on different calli ages (30-, 60- and 90-day-old) of barley, which developed from the same embryo on MS medium supplemented with 3 mg/L 2,4-D. Mature barley embryos (Hordeum vulgare L. cv. Zafer-160) were cultured for callus formation and sub-cultured every 30 days. Three experiment sets were constructed to determine the polymorphism between individual calli originated from different embryos in the same culture time. Polymorphism was detected using Inter-Retrotransposon Amplified Polymorphism (IRAP) technique with two different Nikita specific forward primers. Three mature embryos were used as control. In total, 20 homomorphic PCR bands were obtained from both reactions in intact embryos. However, some polymorphic bands (~ 550 and 650 bp) were solely observed in calli. Our results showed that tissue culture conditions caused the movement of Nikita retrotransposon at different ages of calli that originated from the same embryo and at the same time.
  • Nermin Gözükırmızı 2. Date of Birth : 18.06.1951 3

    Nermin Gözükırmızı 2. Date of Birth : 18.06.1951 3

    1.Name Surname : Nermin Gözükırmızı 2. Date of Birth : 18.06.1951 3. Title : Prof.Dr. 4. State of Education : Prof.Dr. 5. Current Institution : Istinye University Education: BSc. Istanbul University, Faculty of Science, Botany- Zoology, Istanbul, 1972. Ph.D. University of Istanbul, Science Faculty, Department of Botany and Genetics, Istanbul 1979. Dissertation Title: “Effects of some chemicals and rays on Vicia faba”. Assoc. Prof. 1985, University of Istanbul, Science Faculty, Department of Molecular Biology and Genetics Prof. 1992-2018, University of Istanbul, Science Faculty, Department of Molecular Biology and Genetics 6. Academic Appointments Academic Member of Istinye University, Head of Molecular Biology and Genetics Department since September, 2018. Academic Member of Istanbul University since 1972 -2018 June. Bioscience and Biomedical Engineering Faculty, Department of Biosciences and Bioengineering, University Technology, Malaysia, 21.04.2013 - 28.04.2013 The Scientific and Technological Research Council of Turkey , Research Institutes of Genetics Engineering & Biotechnology, Group leader, 1992-2006. Visiting Scientist: Houston University, Biology Dept. 1979-1980. 7. Research Interest: Transposons, Endogenous retroviruses, Long non-coding RNAs Epigenetics, Plant Biotechnology 8. Research Projects and Activities 1. NATO-TU “Biotechnology-Establishment and Promotion of New Biotechnological Techniques in Turkey” Establishment of Tissue Culture and Gene Transfer Systems in Barley 1991-1994. 2. NATO -TU Biotech; "Monoclonal Antibodies- Modification by recombinant DNA Technology and Exploitation", 1994-1998. 3. Plant Biotechnology (TUBITAK)1992-2000 4. Paulownia Tissue Culture and Propagation (TEKNOGEN Comp.)2000 5. Development of Biotechnologicaly Improved Poplar Clones, (Turkish Paper and Pulp Industries)1996-2000. 6. Eureka Cereal Stresstol-1322, 1996-2003. 7. Cotton tissue culture, gene transfer and fiber improvement (Turkish Textile Foundation), 2002-2006.
  • Salt Stress and Homobrassinosteroid Interactions During Germination in Barley Roots Sevgi MARAKLI*, Aslihan TEMEL, Nermin GOZUKIRMIZI

    Salt Stress and Homobrassinosteroid Interactions During Germination in Barley Roots Sevgi MARAKLI*, Aslihan TEMEL, Nermin GOZUKIRMIZI

    Available online: www.notulaebotanicae.ro Print ISSN 0255-965X; Electronic 1842-4309 Not Bot Horti Agrobo, 2014, 42(2):446-452. DOI:10.15835/nbha4229461 Salt Stress and Homobrassinosteroid Interactions during Germination in Barley Roots Sevgi MARAKLI*, Aslihan TEMEL, Nermin GOZUKIRMIZI Istanbul University, Faculty of Science, Department of Molecular Biology and Genetics, 34134, Vezneciler Street-Istanbul, Turkey; [email protected] (*corresponding author) Abstract Potential alleviation effects of Homobrassinosteroid (HBR) (0.5 and 1 µM HBR) on root germination, cell division and antioxidant system enzymes (superoxide dismutase and catalase) of barley (Hordeum vulgare L. cv. ‘Hilal’) roots grown under different salt concentrations (150 mM and 250 mM) were investigated during 48 and 72 h at dark with their controls. Salt applications decreased primary root lengths, seminal root lengths, number of roots from one seed, mitotic activity and induced mitotic abnormalities. In addition, salt application decreased protein content but increased enzyme activities both at 48 h and 72 h when compared to control. Roots treated with HBR enhanced root lengths and root number. HBR-treated roots showed more mitotic activity, mitotic abnormalities and significant enlargements at the root tips when compared to controls and only salt-treated samples. Salt + HBR applications stimulated root lengths and showed more mitotic activity and mitotic abnormalities when compared to only salt-treated samples. Salt application increased superoxide dismutase and catalase activities both at 48 h and 72 h. However, HBR application decreased total soluble protein content and increased enzyme activities especially at 48 h compared to control. Depending on concentration and timing, salt + HBR treatments showed varying results in total soluble protein content and enzyme activities.