Anti- DIABLO Antibody

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anti- DIABLO antibody Product Information Catalog No.: FNab02384 Size: 100μg Form: liquid Purification: Immunogen affinity purified Purity: ≥95% as determined by SDS-PAGE Host: Rabbit Clonality: polyclonal Clone ID: None IsoType: IgG Storage: PBS with 0.02% sodium azide and 50% glycerol pH 7.3, -20℃ for 12 months (Avoid repeated freeze / thaw cycles.) Background Promotes apoptosis by activating caspases in the cytochrome c/Apaf-1/caspase-9 pathway. Acts by opposing the inhibitory activity of inhibitor of apoptosis proteins(IAP). Inhibits the activity of BIRC6/bruce by inhibiting its binding to caspases. Isoform 3 attenuates the stability and apoptosis-inhibiting activity of XIAP/BIRC4 by promoting XIAP/BIRC4 ubiquitination and degradation through the ubiquitin-proteasome pathway. Isoform 3 also disrupts XIAP/BIRC4 interacting with processed caspase-9 and promotes caspase-3 activation. Isoform 1 is defective in the capacity to down-regulate the XIAP/BIRC4 abundance.This antibody recognizes all the three isoforms of DIABLO. Immunogen information Immunogen: diablo homolog(Drosophila) Synonyms: DIABLO, Diablo homolog, mitochondrial, DIABLO S, SMAC, SMAC3 Observed MW: 20 kDa UniprotID : Q9NR28 Application 1 Wuhan Fine Biotech Co., Ltd. B9 Bld, High-Tech Medical Devices Park, No. 818 GaoxinAve.East Lake High-Tech Development Zone.Wuhan, Hubei, China(430206) Tel :( 0086)027-87384275 Fax: (0086)027-87800889 www.fn-test.com Reactivity: Human, Mouse, Rat Tested Application: ELISA, WB, IHC, IP Recommended dilution: WB: 1:500-1:2000; IP: 1:500-1:1000; IHC: 1:20-1:200 Image: Immunohistochemistry of paraffin-embedded human testis using FNab02384(DIABLO antibody) at dilution of 1:50 IP Result of anti-DIABLO (IP:FNab02384, 4ug; Detection:FNab02384 1:500) with HEK-293 cells lysate 2000ug. 2 Wuhan Fine Biotech Co., Ltd. B9 Bld, High-Tech Medical Devices Park, No. 818 GaoxinAve.East Lake High-Tech Development Zone.Wuhan, Hubei, China(430206) Tel :( 0086)027-87384275 Fax: (0086)027-87800889 www.fn-test.com HepG2 cells were subjected to SDS PAGE followed by western blot with FNab02384(DIABLO antibody) at dilution of 1:1000 3 Wuhan Fine Biotech Co., Ltd. B9 Bld, High-Tech Medical Devices Park, No. 818 GaoxinAve.East Lake High-Tech Development Zone.Wuhan, Hubei, China(430206) Tel :( 0086)027-87384275 Fax: (0086)027-87800889 www.fn-test.com .

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Cloning and Sequence Analysis of Canine Apoptosis-Associated Molecules
Zurich Open Repository and Archive University of Zurich Main Library Strickhofstrasse 39 CH-8057 Zurich www.zora.uzh.ch Year: 2007 Cloning and sequence analysis of canine apoptosis-associated molecules Schade, Benjamin Abstract: The aim of the study was to clone and sequence the coding sequences of a representative set of proteins, primarily from the intrinsic apoptotic pathway in dogs and to assess their conservation with hu- man and murine orthologues. cDNA for these proteins, including Bcl-2 family members (Bcl-XL, Bcl-w, Mcl-1, Bax, Bak, Bad, Noxa), caspases (Caspase-3, Caspase-8, Caspase-9), Inhibitors of Apoptosis Pro- teins (XIAP, cIAP-1, cIAP-2, Survivin), their mitochondrial inhibitors (Smac/ DIABLO, Omi/HtrA2) and p53, were generated by RT-PCR with RNA extracted from two canine non-neoplastic cell lines. Eleven sequences are novel for the dog. Interspecies comparison revealed strongest similarity between the sequences of human and canine intrinsic apoptosis pathway members. Differences with potential func- tional impact, however, were observed in both dogs and mice. In dogs, these changes involve the putative Inhibitor of Apoptosis Protein binding motif of canine Omi/HtrA2, some caspase substrate recognition motifs and some functionally relevant residues of p53. Canine XIAP yields a caspase-cleavage site reported as unique to humans. In conclusion, the generally high degree of similarity of canine apoptosis-associated proteins as compared to human counterparts is supportive of the use of dogs as a model for human dis- eases. Single interspecies sequence variations with potential functional relevance under physiologic and neoplastic conditions do exist, however, and will require further analysis.
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Supplemental Table S1
Entrez Gene Symbol Gene Name Affymetrix EST Glomchip SAGE Stanford Literature HPA confirmed Gene ID Profiling profiling Profiling Profiling array profiling confirmed 1 2 A2M alpha-2-macroglobulin 0 0 0 1 0 2 10347 ABCA7 ATP-binding cassette, sub-family A (ABC1), member 7 1 0 0 0 0 3 10350 ABCA9 ATP-binding cassette, sub-family A (ABC1), member 9 1 0 0 0 0 4 10057 ABCC5 ATP-binding cassette, sub-family C (CFTR/MRP), member 5 1 0 0 0 0 5 10060 ABCC9 ATP-binding cassette, sub-family C (CFTR/MRP), member 9 1 0 0 0 0 6 79575 ABHD8 abhydrolase domain containing 8 1 0 0 0 0 7 51225 ABI3 ABI gene family, member 3 1 0 1 0 0 8 29 ABR active BCR-related gene 1 0 0 0 0 9 25841 ABTB2 ankyrin repeat and BTB (POZ) domain containing 2 1 0 1 0 0 10 30 ACAA1 acetyl-Coenzyme A acyltransferase 1 (peroxisomal 3-oxoacyl-Coenzyme A thiol 0 1 0 0 0 11 43 ACHE acetylcholinesterase (Yt blood group) 1 0 0 0 0 12 58 ACTA1 actin, alpha 1, skeletal muscle 0 1 0 0 0 13 60 ACTB actin, beta 01000 1 14 71 ACTG1 actin, gamma 1 0 1 0 0 0 15 81 ACTN4 actinin, alpha 4 0 0 1 1 1 10700177 16 10096 ACTR3 ARP3 actin-related protein 3 homolog (yeast) 0 1 0 0 0 17 94 ACVRL1 activin A receptor type II-like 1 1 0 1 0 0 18 8038 ADAM12 ADAM metallopeptidase domain 12 (meltrin alpha) 1 0 0 0 0 19 8751 ADAM15 ADAM metallopeptidase domain 15 (metargidin) 1 0 0 0 0 20 8728 ADAM19 ADAM metallopeptidase domain 19 (meltrin beta) 1 0 0 0 0 21 81792 ADAMTS12 ADAM metallopeptidase with thrombospondin type 1 motif, 12 1 0 0 0 0 22 9507 ADAMTS4 ADAM metallopeptidase with thrombospondin type 1
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