toxins Article Molecular Identification and Toxin Analysis of Alexandrium spp. in the Beibu Gulf: First Report of Toxic A. tamiyavanichii in Chinese Coastal Waters Yixiao Xu 1,2,* , Xilin He 1, Huiling Li 1, Teng Zhang 1, Fu Lei 3, Haifeng Gu 4 and Donald M. Anderson 5 1 Key Laboratory of Environment Change and Resources Use in Beibu Gulf, Ministry of Education, Nanning Normal University, Nanning 530001, China;
[email protected] (X.H.);
[email protected] (H.L.);
[email protected] (T.Z.) 2 Guangxi Laboratory on the Study of Coral Reefs in the South China Sea, Guangxi University, Nanning 530004, China 3 Guangxi Key Laboratory of Marine Environmental Science, Beibu Gulf Marine Research Center, Guangxi Academy of Sciences, Nanning 530007, China;
[email protected] 4 Third Institute of Oceanography, Ministry of Natural Resources, Xiamen 361005, China;
[email protected] 5 Biology Department, Woods Hole Oceanographic Institution, Woods Hole, MA 02543, USA;
[email protected] * Correspondence:
[email protected] Abstract: The frequency of harmful algal blooms (HABs) has increased in China in recent years. Information about harmful dinoflagellates and paralytic shellfish toxins (PSTs) is still limited in China, especially in the Beibu Gulf, where PSTs in shellfish have exceeded food safety guidelines on multiple occasions. To explore the nature of the threat from PSTs in the region, eight Alexandrium strains were isolated from waters of the Beibu Gulf and examined using phylogenetic analyses of large subunit (LSU) rDNA, small subunit (SSU) rDNA, and internal transcribed spacer (ITS) sequences. Their toxin composition profiles were also determined using liquid chromatography-tandem mass spectrometry Citation: Xu, Y.; He, X.; Li, H.; (LC-MS/MS).