EPHA4 Sirna Set I EPHA4 Sirna Set I

Catalog # Aliquot Size E16-911-05 3 x 5 nmol E16-911-20 3 x 20 nmol E16-911-50 3 x 50 nmol EPHA4 siRNA Set I siRNA duplexes targeted against three exon regions Catalog # E16-911 Lot # Z2037-21 Specificity Formulation EPHA4 siRNAs are designed to specifically knock-down The siRNAs are supplied as a lyophilized powder and human EPHA4 expression. shipped at room temperature. Product Description Reconstitution Protocol EPHA4 siRNA is a pool of three individual synthetic siRNA Briefly centrifuge the tubes (maximum RCF 4,000g) to duplexes designed to knock-down human EPHA4 mRNA collect lyophilized siRNA at the bottom of the tube. expression. Each siRNA is 19-25 bases in length. The gene Resuspend the siRNA in 50 µl of DEPC-treated water accession number is NM_004438. (supplied by researcher), which results in a 1x stock solution (10 µM). Gently pipet the solution 3-5 times to mix Gene Aliases and avoid the introduction of bubbles. Optional: aliquot SEK, HEK8, TYRO1 1x stock solutions for storage. Storage and Stability Related Products The lyophilized powder is stable for at least 4 weeks at room temperature. It is recommended that the Product Name Catalog Number lyophilized and resuspended siRNAs are stored at or EPHA1, Active E13-11G below -20oC. After resuspension, siRNA stock solutions ≥2 EPHA1, Active E13-18G µM can undergo up to 50 freeze-thaw cycles without EPHA2, Active E14-11G significant degradation. For long-term storage, it is EPHA3, Active E15-11G recommended that the siRNA is stored at -70oC. For most EPHA4, Active E16-11G favorable performance, avoid repeated handling and EPHA6, Active E18-11G multiple freeze/thaw cycles. EPHA7, Active E19-11G EPHA8 Protein E20-35G Scientific Background EPHA4 also known as EPH receptor A4, belongs to the ephrin receptor subfamily of protein-tyrosine kinases which have been implicated in mediating EPHA4 siRNA Set I siRNA duplexes targeted against three exon regions developmental events, particularly in the nervous system (1). The EPHA4 ligand ephrin-A3 is localized to the Catalog Number E16-911 astrocytic processes that envelop the spine. Activation of Specific Lot Number Z2037-21 EPHA4 by ephrin-A3 induces spinal retraction and Packaging Specifications 2.5 nmol/tube for 3 x 5 nmol reduces spine density and inhibits the interaction Format Lyophilized powder distorted spine shape and organization. EPHA4-null mice Stability 1yr at -70oC from date of shipment possess defects in the corticospinal tract and anterior Storage & Shipping The lyophilized powder is stable for at commissure indicating a model in which an ephrin ligand least 4 weeks at room temperature. It is recommended that the lyophilized on the axons senses EPHA4 on spinal cord cells and resuspended siRNAs are stored at surrounding the corticospinal tract (2). or below -20oC. After resuspension, siRNA stock solutions ≥2 µM can undergo up to 50 freeze-thaw cycles References without significant degradation. For 1. Flanagan, J.G. et al: The ephrins and Eph receptors in long-term storage, it is recommended neural development.". Annu. Rev. Neurosci. 1998;21: that the siRNA is stored at -70oC. For most favorable performance, avoid 309–45. repeated handling and multiple 2. Dottori, M. et al: EphA4 (Sek1) receptor tyrosine freeze/thaw cycles. kinase is required for the development of the corticospinal tract. Proc. Nat. Acad. Sci. 95: 13248- 13253, 1998. To place your order, please contact us by phone 1-(604)-232-4600, fax 1-604-232-4601 or by email: [email protected] www.signalchem.com FOR IN VITRO RESEARCH PURPOSES ONLY. NOT INTENDED FOR USE IN HUMAN OR ANIMALS. .

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