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US 20140377385A1 (19) United States (12) Patent Application Publication (10) Pub. No.: US 2014/0377385 A1 ENAN (43) Pub. Date: Dec. 25, 2014 (54) PEST CONTROL COMPOSITIONS AND AOIN35/04 (2006.01) METHODS AOIN 43/78 (2006.01) AOIN 43/88 (2006.01) (71) Applicant: TyraTech, Inc., Morrisville, NC (US) AOIN 6.5/22 (2006.01) AOIN 65/16 (2006.01) (72) Inventor: Essam ENAN, Davis, CA (US) AOIN37/02 (2006.01) AOIN 65/12 (2006.01) 1-1. (52) U.S. Cl. (22) Filed: May 29, 2014 CPC ................ A0IN 65/32 (2013.01); A0IN 65/12 O O (2013.01); A0IN 65/08 (2013.01); A0IN37/18 Related U.S. Application Data (2013.01); A0IN37/36 (2013.01); A0IN 27/00 (63) Continuation of application No. 12/009.220, filed on (2013.01); A0IN 43/50 (2013.01); A0IN 43/78 Jan. 16, 2008, now abandoned. (2013.01); A0IN 43/88 (2013.01); A0IN 65/22 (60) Provisional application No. 60/885,214, filed on Jan. (2013.01); "N'e','!N 7e 16, 2007, provisional application No. 60/885,403, • u. fs filed on Jan. 17, 2007, provisional application No. USPC ............................ 424/745; 424/764: 514/341 60/889,259, filed on Feb. 9, 2007. (57) ABSTRACT Publication Classification Embodiments of the present invention provide compositions for controlling a target pest including a pest control product (51) Int. Cl. and at least one active agent, wherein: the active agent can be AOIN 65/32 (2006.01) capable of interacting with a receptor in the target pest; the AOIN 65/08 (2006.01) pest control product can have a first activity against the target AOIN37/18 (2006.01) pest when applied without the active agent and the composi AOIN 37/36 (2006.01) tions can have a second activity against the target pest, and the AOIN 27/00 (2006.01) second activity can be greater than the first activity. Patent Application Publication Dec. 25, 2014 Sheet 1 of 15 US 2014/0377385 A1 Patent Application Publication Dec. 25, 2014 Sheet 2 of 15 US 2014/0377385 A1 Patent Application Publication Dec. 25, 2014 Sheet 3 of 15 US 2014/0377385 A1 PKC Biogenic amine receptors coupled Ca?: to intracellular P3/DAG signating pathway FIG. 3 Patent Application Publication Dec. 25, 2014 Sheet 4 of 15 US 2014/0377385 A1 % o s e d s s wn Ø s s 3,s o SSSSSSSSSSSSSSSSSSNS s (s SN %% N & d Š s N O SS c a. SSSNSN 3, % T & s SSNSS SNSS 3, s E o3 NS 2 c s s 2S qa : re SSSS S 2, w NSNS w 3, s ss s E SNSN 2. ra s co s n o a SeSN g d o d k g d r N KD Patent Application Publication Dec. 25, 2014 Sheet 5 of 15 US 2014/0377385 A1 Middle Dose of H1 with Clothianidin: % Knock Down at 30 seconds Post Application of 1 m of Test Material: Aedes aegypti. 120 Treatment FIG. 4B Patent Application Publication Dec. 25, 2014 Sheet 6 of 15 US 2014/0377385 A1 % Knock Down at 30 seconds Post Application of 1 ml of Test Material: Aedes aegypti. 120 NF.N Hists 2 % Ns N Treatment FG. 4C Patent Application Publication Dec. 25, 2014 Sheet 7 of 15 US 2014/0377385 A1 % Knock Down at 30 seconds Post Application of 1 ml of Test Material: Drosophila sp. 80 Treatment FIG. 4D Patent Application Publication Dec. 25, 2014 Sheet 8 of 15 US 2014/0377385 A1 o % E grew ca3. c SN %: 8 c s 2 s d e. a o f. d es O S. 3ver Näs;% s es ea y SN-2 s e s NSS3, 2 Š9 SS r " S. g E es s s N s waus wed s c c s o N ce es c 3. % KD Patent Application Publication Dec. 25, 2014 Sheet 9 Of 15 US 2014/0377385 A1 © %), F.G. 6A /0377385 A1 Synergy Test for American Cockroa ch 1:100, 1:50, :500 imidacloprid/5% HL-1 ````* ```` N ````§ ```` §º2§,ºg,ºg F.G. 6B Patent Application Publication Dec. 25, 2014 Sheet 11 of 15 US 2014/0377385 A1 20 00 s 80 o 60 CL4 e - O - pyrethrum 40 -v- mix t 20 as 0 2 4 6 8 10 12 14 16 18 20 22 24 Time elapsed after treatment, hrs FIG. 7 Patent Application Publication Dec. 25, 2014 Sheet 12 of 15 US 2014/0377385 A1 +?————————————————r————---+ +---+---+-------------------------~--~~~~~~~~~~~~~r????????????????????+ +——————————~~~~~~——————---+ +———————————————————————H +————————––1––1––1–––1––1––1–––4 +————————–––1––––––1––1––? Patent Application Publication Dec. 25, 2014 Sheet 13 of 15 US 2014/0377385 A1 +~~~~~~~~~~~~~~~~~} +—————————~~~~f~~~~—~~~~} +—————————+–––1––1––1––––1–––? Patent Application Publication Dec. 25, 2014 Sheet 14 of 15 US 2014/0377385 A1 |||||()|| 00089 00087 ||||||||\(|||||||||||()||()|| RFU Patent Application Publication Dec. 25, 2014 Sheet 15 of 15 US 2014/0377385 A1 |`?)|| |||()|| ||---- 0802090y0Z010 > ?I |(-)|T(S)>||||| ||||||()||NL|||| |No.|||| 00000}, 0 US 2014/0377385 A1 Dec. 25, 2014 PEST CONTROL COMPOSITIONS AND target pest at a standard concentration of pest control product, METHODS and the compositions exhibit a greater disablement effect than the pest control product applied without the active agent. The CROSS-REFERENCE TO RELATED first activity can persist for a first period, the second activity APPLICATIONS can persist for a second period, and the second period can be 0001. This application is a continuation of U.S. patent longer than the first period. The active agent can include a application Ser. No. 12/009,220, filed Jan. 16, 2008, which synergistic combination of at least two receptor ligands. The claims priority from U.S. Provisional Application Ser. Nos. second activity can reflect a synergistic interaction of the 60/885,214 filed Jan. 16, 2007, 60/885,403 filed Jan. 17, active agent and the pest control product. 2007, and 60/889,259 filed Feb. 9, 2007, each of which is 0007. The target pest can be selected from the group con hereby incorporated by reference in its entirety. sisting of a fungus, a plant, an animal, a moneran, and a protist. The target pest can be an arthropod species, such as, FIELD OF THE INVENTION for example, an insect, an arachnid, or an arachnoid. The target pest can be a species belonging to an animal order 0002 The present invention relates to compositions and selected from: Acari, Anoplura, Araneae, Blattodea, methods related to controlling insects. Coleoptera, Collembola, Diptera, Grylloptera, Heteroptera, Homoptera, Hymenoptera, Isopoda, Isoptera, Lepidoptera, BACKGROUND OF THE INVENTION Mantodea, Mallophaga, Neuroptera, Odonata, Orthoptera, 0003. While the first recorded use of chemicals to control Psocoptera, Siphonaptera, Symphyla, Thysanura, and Thys pests dates back to 2500 BC, only in the last 60 years has anoptera. chemical control has been widely used. Early pesticides 0008. The pest control product can be a chlorphenoxy included hellebore to control body lice, nicotine to control compound Such as, for example, 2,4-D Amine and/or 2.4D aphids, and pyrithrinto control a wide variety of insects. Lead IBE. Likewise, the pest control product can be a carbamate arsenate was first used in 1892 as an orchard spray, while at Such as, for example, methomyl, carbofuran, carbaryl, the same time it was discovered that a mixture of lime and BPMC, carbendazim, carbosulfan, captain hydrochloride, copper sulphate (Bordeaux mixture) controlled downy mil and/or cartap. The pest control product can be an organophos dew, a fungal disease of grapes. phate such as, for example, acephate, malathion, diazinon, 0004. The modern era of chemical pest control com chlorpy firos, fenoxycab, edifenphos, febuconazole, chlor menced during World War II. For example, DDT played a phenapyr, magnesium phosphide, metamidophos, and/or major role in maintaining the health and welfare of Soldiers fenitrothion. The pest control product can be an organochlo who used it to control body lice and mosquitoes. Further rine such as, for example, DDT. DDE, and/or heptachlorep developments of pesticides followed, and with their relatively oxide. The pest control product can be a pyrethroid such as, low cost, ease of use, and effectiveness, they became the for example, cypermethrin, cynmethylin +2,4-D IBE, lamb primary means of pest control. Protection of crops, produce, dacyhalothrin, dazomet, cyfluthrin, betacypermethrin, pen animals, and humans over extended periods became possible dimethlin, permethrin, deltamethrin, bifenethrin, alphacyper with corresponding increases in food production and methrin, fenvalerate, propanil, and/or esfenvalerate. The pest improved standards of living. control product can be a neonicotinoid such as, for example, 0005. Some modern pesticides are sophisticated com thiomethoxam, fipronil, clothianidin, and/or imidacloprid. pounds that are carefully researched to ensure they are effec The pest control product can include at least one of an aver tive against target organisms, generally safe to the environ mectin, abamectin, spinosad, fluxastrobin, and/or indoxac ment, and can be used without undue hazard to users or arb. The pest control product can be a botanical product Such consumers. Many of these have been developed to target as, for example, rotenone, nicotine, caffeine, a pyrethrum, an specific biochemical reactions within the target organism, essential oil, and/or a fixed oil. The pest control product can e.g. an enzyme necessary for photosynthesis within a plant or be a fungicide, a nematicide, an insecticide, an acaricide, a hormone required for normal development in an insect. and/or a bactericide. Thus, Some modern chemicals are safer, more specific, and 0009. The receptor can be a G protein-coupled receptor friendlier to the environment than the older products they (GPCR), such as a GPCR of the insect olfactory cascade, such have replaced. as, for example, a tyramine receptor, an olfactory receptor Or43a, an olfactory receptor Or83b and/or an octopamine SUMMARY receptor.
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