Polyclonal Antibody to AMFR / RNF45 (N-Term) - Purified

OriGene Technologies, Inc. OriGene Technologies GmbH

9620 Medical Center Drive, Ste 200 Schillerstr. 5 Rockville, MD 20850 32052 Herford UNITED STATES GERMANY Phone: +1-888-267-4436 Phone: +49-5221-34606-0 Fax: +1-301-340-8606 Fax: +49-5221-34606-11 [email protected] [email protected]

AP12067PU-N Polyclonal Antibody to AMFR / RNF45 (N-term) - Purified

Alternate names: AMF receptor isoform 2, Autocrine motility factor receptor isoform 2, RING finger protein 45, gp78 Quantity: 0.4 ml Concentration: lot specific Background: Autocrine motility factor (AMF) is a protein secreted by tumor cells that stimulates tumor motility. The gene for AMFR encodes a 323-amino acid polypeptide that has a single transmembrane domain and several putative glycosylation sites. The protein sequence has some homology to human tumor protein p53. Uniprot ID: Q9UKV5 NCBI: NP_001135 GeneID: 267 Host / Isotype: Rabbit / Ig Immunogen: This antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the N-terminal region of human AMFR. Format: State: Liquid purified Ig fraction. Purification: Protein G Chromatography, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS. Buffer System: PBS with 0.09% (W/V) Sodium Azide as preservative. Applications: ELISA: 1/1,000. Western Blot: 1/100-1/500. Immunohistochemistry: 1/50-1/100. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user. Specificity: This antibody is specific to AMFR (N-term). Species: Human. Other species not tested. Add. Information: Predicted Molecular weight: 34325 Da Storage: Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing. Shelf life: one year from despatch. Caution: This product is for research use only. Not for use in diagnostic or therapeutic procedures. General Readings: 1. Kenichiro Araki, et al. J. Biol. Chem. 2009; 284:32305-32311. 2. Huang, B., et al., Biochem. Biophys. Res. Commun. 212(3):727-742 (1995). 3. Watanabe, H., et al., J. Biol. Chem. 266(20):13442-13448 (1991).

For research and in vitro use only. Not for diagnostic or therapeutic work. Material Safety Datasheets are available at www.acris-antibodies.com or on request. OG/20130805 1 / 2 AP12067PU-N: Polyclonal Antibody to AMFR / RNF45 (N-term) - Purified

Pictures: (LEFT) Formalin-fixed and paraffin- embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. (RIGHT) Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with AMFR Antibody (N-term) (Cat.#AP12067PU-N), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. (LEFT) Western blot analysis of anti-AMFR Antibody (N-term) (Cat.#AP12067PU-N) in CEM cell line lysate (35ug/lane). AMFR (arrow) was detected using the purified Pab. (RIGHT) Western blot analysis of AMFR (arrow) using AMFR Antibody (N- term) (Cat.#AP12067PU-N). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the AMFR gene (Lane 2) (Origene Technologies).

For research and in vitro use only. Not for diagnostic or therapeutic work. Material Safety Datasheets are available at www.acris-antibodies.com or on request. OG/20130805 2 / 2