Review of Synthetic Human Faeces and Faecal Sludge for Sanitation and Wastewater Research
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Water Research 132 (2018) 222e240 Contents lists available at ScienceDirect Water Research journal homepage: www.elsevier.com/locate/watres Review Review of synthetic human faeces and faecal sludge for sanitation and wastewater research * Roni Penn a, , Barbara J. Ward a, Linda Strande a, Max Maurer a, b a Eawag, Swiss Federal Institute of Aquatic Science and Technology, 8600 Dübendorf, Switzerland b Institute of Civil, Environmental and Geomatic Engineering, ETH Zürich, 8093 Zurich, Switzerland article info abstract Article history: Investigations involving human faeces and faecal sludge are of great importance for urban sanitation, Received 17 July 2017 such as operation and maintenance of sewer systems, or implementation of faecal sludge management. Received in revised form However, working with real faecal matter is difficult as it not only involves working with a pathogenic, 22 December 2017 malodorous material but also individual faeces and faecal sludge samples are highly variable, making it Accepted 23 December 2017 difficult to execute repeatable experiments. Synthetic faeces and faecal sludge can provide consistently Available online 30 December 2017 reproducible substrate and alleviate these challenges. A critical literature review of simulants developed for various wastewater and faecal sludge related research is provided. Most individual studies sought to Keywords: fi Fecal sludge develop a simulant representative of speci c physical, chemical, or thermal properties depending on Fecal sludge simulant their research objectives. Based on the review, a suitable simulant can be chosen and used or further Feces developed according to the research needs. As an example, the authors present such a modification for Feces simulants the development of a simulant that can be used for investigating the motion (movement, settling and Onsite wastewater treatment sedimentation) of faeces and their physical and biological disintegration in sewers and in on-site sani- Sewers tation systems. © 2018 Elsevier Ltd. All rights reserved. Contents 1. Introduction . ....................... 223 2. Characteristics of human faeces and faecal sludge . ....................... 223 2.1. Faeces .................................................................... ..................................................223 2.2. Faecal sludge . ..................................................224 3. Synthetic faeces and faecal sludge found in the literature . ....................... 224 3.1. Physical parameters . ..........................................224 3.1.1. Faeces simulants . ..........................................225 3.1.2. Faecal sludge simulants . ..........................................226 3.2. Chemical, biological, and thermal parameters . ......................................228 3.2.1. Faeces simulants . ..........................................228 3.2.2. Faecal sludge simulants . ..........................................229 4. Discussion . ....................... 230 4.1. Making use of Table 8 for development of a new simulant . ...........................233 4.1.1. New faeces simulant . ..........................................233 4.1.2. New faecal sludge simulant . ..........................................233 5. Conclusions . ....................... 234 Development of new simulant . ....................... 235 Material and methods . ..........................................235 Chemicals and materials used . ..........................................235 Measurement methods . ..........................................235 * Corresponding author. E-mail address: [email protected] (R. Penn). https://doi.org/10.1016/j.watres.2017.12.063 0043-1354/© 2018 Elsevier Ltd. All rights reserved. R. Penn et al. / Water Research 132 (2018) 222e240 223 Base synthetic faeces recipe . .......................235 Experiments . .......................235 Results and discussion . .......................236 Physical structure . .......................236 Chemical composition . .......................238 Recommendation for recipes . .......................238 Recommended storage practices . .......................238 Synthetic faeces production . ................................................ 239 References.................................................................................................. .......................239 1. Introduction disintegration. For these kinds of investigations the simulant is required to obtain a combination of chemical, biological and Investigations involving human faeces are of great importance physical properties. Such a faeces simulant is still missing in the in many fields of research, such as medicine (Lewis and Heaton, literature. 1997; Bekkali et al., 2009), sanitary product development (such as Three distinct recipes for synthetic faecal sludge have been re- diapers, toilets etc.) (Stern and Holtman, 1987; Palumbo and ported in the literature. Their intended purposes include research D'acchioli, 2001), operation and maintenance of sewer systems into anaerobic digestion (Zuma, 2013; Colon et al., 2015) and pit (Butler et al., 2003; Penn et al., 2017), and implementation of faecal latrine emptying (Radford et al., 2015). Together with the synthetic sludge collection and treatment for onsite sanitation systems faeces recipes presented in this review, they could be used as a basis (Wignarajah et al., 2006; Bassan et al., 2014; Colon et al., 2015). for the development of improved faecal sludge simulants in the Development of synthetic faeces and faecal sludge is a challenging future. task due to their high variability depending on diet, lifestyle and In this article, we provide a critical literature review of synthetic geographical location (Rose et al., 2015). In this paper, we focus on faeces and faecal sludge used for human waste related research. synthetic faeces and faecal sludge developed for sanitation Based on this overview we present a modified simulant recipe that research, hence resembling human stool and faecal sludge in spe- is applicable to be used for studying the fate of faeces in sewers and cific physical and chemical properties. The high variability of faeces in onsite sanitation systems. A series of experimental results (Rose et al., 2015) and faecal sludge collected from onsite systems showing how these properties can be selectively manipulated by (Strande et al., 2014) makes it difficult to obtain consistent samples making changes in the recipe and an explicit preparation procedure and therefore execute repeatable experiments. Moreover, due to can be found in the appendix of this paper. the potentially pathogenic content of human excreta, working with real faecal matter involves special safety precautions. Working with 2. Characteristics of human faeces and faecal sludge synthetic faecal matter can alleviate these challenges. Faeces and faecal sludge are different. Faecal sludge is the faecal 2.1. Faeces waste stored within onsite sanitation technologies. In addition to faeces it includes everything that goes into the toilet, for example, Faecal solids are composed of proteins, fats, fibre, bacterial urine, flush water, greywater, anal cleansing materials and munic- biomass, inorganic materials and carbohydrates. Their chemical ipal solid waste (Strande et al., 2014). Faecal sludge differs signifi- and physical characteristics vary widely depending on person's cantly from fresh faeces alone; it is typically much more dilute due health and diet, as presented in Table 1. The average number of to the addition of liquids. Additionally, its characteristics are highly stools produced by adults is one per day (Cibae Geigy, 1977). The variable due to differences in storage duration, storage temperature median daily wet mass of faeces produced per person is 128 g (Rose and storage technology, and can range from fresh, to partially et al., 2015), which falls within the reported full range of 35e796 g degraded, to completely stabilized (Strande et al., 2014). Synthetic reported by Ciba e Geigy (1977) and Rose et al. (2015). Wyman et al. faeces have been developed to address many sanitation related (1978) compared average stool sizes of 20 people (average of 10 research questions. Most of the developed simulants mimic specific samples from each individual). They identified that 250 g/stool and physical, chemical or thermal characteristics of human faeces 111.3 g/stool were the maximum of these averaged weights of the important to the research objectives for which they are developed. men and women participants, respectively, in the study. In their Physical properties such as shape, size, density and rheology are of review of faeces characteristics Rose et al. (2015) further report that importance for simulating phenomena such as faeces settling, live and dead bacteria comprise between 25 and 54% of the dry transport in sewer pipes, dewatering, viscous heating for pathogen weight of faeces. The median water content in faeces is 75%, with a destruction, and physical disintegration (e.g., Butler et al., 2003; range of 63e86% across mean values of studies. Variations in water Veritec Consulting Inc. & Koeller and Company, 2010; Podichetty content and faecal mass are attributed to differences in fibre intake, et al., 2014). Chemical properties including chemical and biolog- as non-degradable fibre absorbs more water in the colon and ical oxygen demand, nutrient concentration, pH and conductivity degradable fibre stimulates growth of bacterial biomass (Eastwood, are of importance for simulating biological disintegration,