05686 DMACA (Indol Detection Disks)

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05686 DMACA (Indol Detection Disks) The DMACA Indole discs are used for Indole test to determine the ability of an organism to split tryptophan into indole and α-aminopropionic acid. The presence of indole can be detected by the addition of DMACA which results in a bluish-purple complex. With this method it is possible to differentiate Escherichia coli from Klebsiella. Composition: (1 package contains 50 disks) The discs contain p-Dimethylaminocinnamaldehyde (DMACA). Directions: Place the disc on suspected colony from e.g. UTI ChromoSelect Agar, modified (16636) or Christensen’s Urea Agar (27048) plate on filter paper. Observe for appearance of blue-purple color within 10-30 seconds. Quality control: Test Organisms (ATCC) DMACA Escherichia coli (25922) + Pseudomonas aeruginosa (27853) - Klebsiella pneumoniae (13883) - 1. Staphylococcus aureus 2. Escherichia coli Page 1 of 2 References: 1. R. Vracko, J.C. Sherris, Indole-spot test in bacteriology., Am. J. Clin. Pathol., 39, 429 (1963) 2. V.L. Sutter, W.T. Carter, Evaluation of media and reagents for indole-spot test in anaerobic bacteriology., Am. J. Clin. Pathol., 58, 335 (1972) 3. G.D. Fay, A.L. Barry, Methods for detecting indole production by gram-negative nonsporeforming anaerobes., Appl. Micro. 27, 562 (1974) 4. D.F. Welch, P.A. Ahlin, J.M. Matsen, Differentiation of Haemophilus spp. in respiratory isolate cultures by an indole spot test., J. Clin. Micro. 15, 216 (1982) 5. H.D. Isenberg, Ed., Clinical microbiology procedures handbook, Vol 1., Washington, DC, ASM (1992) 6. B.A. Forbes, D.F. Sahm, A.S. Weissfeld, Bailey and Scott's diagnostic microbiology., 10th ed., St Louis, Mosby (1998) 7. J.F. MacFaddin, Biochemical tests for identification of medical bacteria., 3rd ed., Philadelphia, Lippincott Williams & Wilkins (2000) Precautions and Disclaimer This product is for R&D use only, not for drug, household, or other uses. Please consult the Material Safety Data Sheet for information regarding hazards and safe handling practices. The vibrant M, Millipore, and Sigma-Aldrich are trademarks of Merck KGaA, Darmstadt, Germany or its affiliates. Detailed information on trademarks is available via publicly accessible resources. © 2018 Merck KGaA, Darmstadt, Germany and/or its affiliates. All Rights Reserved. Page 2 of 2 .

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Characterization and Antibiotic Sensitivity Profile of Bacteria Isolated from Patients with Respiratory Tract Infections in Bangladesh
Characterization and Antibiotic Sensitivity Profile of Bacteria Isolated from Patients with Respiratory Tract Infections in Bangladesh Shukla Promite1, Sajal K. Saha2, Sunjukta Ahsan1 and Marufa Zerin Akhter1 1Department of Microbiology, University of Dhaka, Dhaka, Bangladesh 2Department of General Practice, Monash University, Building 1, 270 Ferntree Gully Road, Notting Hill VIC 3168, Australia (Received: October 08, 2017; Accepted: December 15, 2017; Published (web): December 23, 2017) ABSTRACT: The study was aimed to characterize bacterial isolates from respiratory tract infections (RTI) and investigate their antibiotic sensitivity profile. Selective media and biochemical tests were used to characterize 40 bacterial isolates. Antibiotic sensitivity testing was conducted using Kirby-Bauer disc diffusion method. About 42.5% (17) RTI patients were infected by Klebsiella pneumoniae, 30% (12) by Escherichia coli and 27.5% (11) by Pseudomonas aeruginosa with no significant gender variation (p-value <0.578). Overall, 47% (out of 20) antibiotics were sensitive, whereas 48% were resistant. Surprisingly, 18% P. aeruginosa and 20% K. pneumoniae were carbapenem-resistant and 4 out of 7 cephalosporin antibiotics were highly resistant irrespective of pathogens. E. coli showed better sensitivity to nitrofurantoin (78%) and levofloxacin (89%), while K. pneumoniae was insensitive to cotrimoxazole (88%), gentamycin (77%) and piperacillin/tazobactam (66%). On the other hand, P. aeruginosa did not respond to P. aeruginosa to nalidixic acid (60%) and ciprofloxacin (60%). This study concludes that nitrofurantoin, levofloxacin, cotrimoxazole, gentamycin and piperacillin/tazobactam antibiotics could be better alternative in treating bacterial RTIs. Key words: Antibiotic sensitivity, bacterial pathogens, RTIs, Bangladesh. INTRODUCTION Antibiotic resistance (AR) is a global public The rise of AR in Bangladesh is probably due to 1 health concern.
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