Rapid Tests and Reagents Microbiology Time Saving Tests

Total Page:16

File Type:pdf, Size:1020Kb

Rapid Tests and Reagents Microbiology Time Saving Tests Rapid Tests and Reagents Microbiology Time Saving Tests Manufactured by Rapid Tests and Reagents Microbiology Time Saving Tests Rapid Tests and Reagents Bile, SpotDrops™ Deoxycholate 10%, bile solubility test for Streptococcus pneumoniae. 15ml, Each .......... 10790-366 BlueMount™ A permanent mounting fluid and preservative that contains the stain lactophenol cotton blue. 15ml, Each .......... 10324-560 Butyrate See CatScreen™. C. diff PRO™ C. diff PRO™ Card Test Kit is recommended for use Albiquick™, for Candida albicans, Cat. no. 10324-428. in screening suspected AlbiQuick™ Beta-Lactamase, Chromogenic Clostridium difficile isolates A rapid five minute enzymatic See Nitrocef. for L-proline aminopeptidase card test to aid in the activity and a lack of indole ™ identification of Candida Bile, HardyDisks activity. albicans. (See image below.) To aid in the identification of 25 tests/kit ........... 10053-794 25 tests ..................10324-428 Bacteroides fragilis group on the basis of bile resistance. Campylobacter Anaerobe Identification 50 disks/cartridge .. 89426-240 See Campylobacter Latex. See Bile and Nitrate. Bacillus ID Panel For the identification of Bacillus spp. and related genera, Microgen® 20 tests/kit ............10032-748 Bacitracin, HardyDisks™ For the differentiation of Group A Strep, 0.04 Units (A disk). 5x50 disks/ cartridge .............. 10790-374 Bacteroides See Nitrocef. C. diff PRO™, aids in C. difficile identification, Cat. no. 10053-794. Rapid Tests & Reagents 1 Campylobacter Latex Rapid latex agglutination slide test for enteropathogenic Campylobacter grown from selective media. 50 tests/kit ........... 10062-766 Candida albicans See AlbiQuick™ and Germ Tube Cryo™. Candida glabrata See Trehalose, Rapid. Catalase, 3% Hydrogen peroxide 3%, detects catalase activity. ™ 15ml, Each ..........89407-446 CarboFerm , Cat. no. 10324-438, for Neisseria spp. and Moraxella catarrhalis. 60ml, Each ..........89407-444 Catalase, 15% CatScreen™ CarboFerm™ Hydrogen peroxide 15%, Rapid test for the detection of For rapid identification of detects bacterial catalase the enzyme butyrate esterase Neisseria gonorrhoeae, activity in anaerobes. in bacteria isolated on culture Neisseria meningitidis, 15ml, Each .......... 10063-756 media for the presumptive Moraxella catarrhalis, and identification of Branhamella other Neisseria spp. (Moraxella) catarrhalis. • Results in 2-4 hours. Performs a butyrate test within • Easy-to-read color change. 5 minutes. Simply moisten • Includes interpretation disk, inoculate with organism, guide and results pad. then wait for the development 24 tests ................ 10324-438 of a red color. Cefinase 25 disks tests/pk ... 10324-426 See Nitrocef. Clostridium difficile Latex Clostridium difficile latex agglutination slide test from culture, by Microgen. 50 tests/kit ........... 10062-776 Coagulase Cryo™ A ready-to-use vial of frozen coagulase rabbit plasma with EDTA for ID of Staphylococcus aureus, 0.5ml, 20 tests ................89407-462 BlueMount™, Cat. no. 10324-560, permanent Mycology stain. 2 Rapid Tests & Reagents CoaguStaph™ A lyophilized preparation of coagulase rabbit plasma with EDTA for the ID of Staphylococcus aureus. 6x3ml .................. 89407-458 6x20ml ................89407-460 ColiScreen™ A rapid test method for the identification of E. coli; because indole alone is not enough to identify E. coli. Performs an indole and glucuronidase test in one tube. Takes 1 to 4 hours. Requires Coagulase Cryo™, Cat. No. 89407-462, aids in Staphylococcus indole spot test reagent, Cat. aureus identification. no. 89428-932 which is not included. E. coliPRO™ O157 Latex Enterococcus 13x100mm, 0.3ml, A rapid latex agglutination test See PYR. 20 tests/pk ..........10789-402 kit for the identification of E. coli O157. Uses EnteroScreen 4™ Cryptococcus Test proprietary antibodies that Enables the screening of See India Ink. eliminate the possibility of enteric pathogens from plated E. coli cross-reactions with Escherichia stool specimens, and the See ColiScreen™ and Indole. hermanii and other sorbitol- differentiation of Salmonella negative Escherichia species. and Shigella from normal 50 tests ................ 89407-502 enteric flora in one tube. 16x125mm tube, CatScreen™, Cat. 20 tubes/bx ......... 10324-472 no. 10324-426, for Moraxella Ferric Chloride, 10% catarrhalis. Developer for tryptophan and Read-out in only phenylalanine deaminase five minutes! tests. 15ml, Each .......... 89407-514 Flagella Stain Stain for bacterial flagella. 15ml, Each .......... 89407-516 Gardnerella vaginalis See Hippurate Test, Metronidazole, and Sulfonamide. Rapid Tests & Reagents 3 Germ Tube Cryo™ Iodine-Iodide Solution A ready-to-use frozen vial with For use with Tetrathionate serum for Candida albicans Broth, Cat. no. 89407-968. germ tube test, 0.5ml. 50ml, Each .......... 89407-624 20 vials/pk .......... 10324-568 1 liter polycarbonate bottle, Gram Test 990ml fill, Each ....10053-840 See LanaGram™. KOH, 10% Haemophilus spp. Potassium hydroxide for fungal wet mounts. See ALA Disks, Indole Rapid, 15ml, Each .......... 10063-758 CoaguStaph™, Cat. and XV Factor Disks. no. 89407-458, for LAP Staphylococcus aureus. Helicobacter pylori Intended for the preliminary See Urea Medium, Rapid. identification of catalase- Lactophenol Cotton Blue India Ink negative, gram-positive cocci. Stain for fungal wet mounts, LAP positive organisms include Negative stain for capsule also see BlueMount™. Pediococci, Lactococci, detection in Cryptococcus 15ml, Each .......... 89428-922 Enterococci, Streptococcus neoformans and pneumoniae, and ™ Cryptococcus gattii. LanaGram Streptococcus pyogenes; while A rapid enzymatic test to 15ml, Each .......... 89428-916 other ß-hemolytic streptococci, assist in the determination of Indole, Kovac’s Aerococcus and Leuconostoc the Gram reaction in difficult Benzaldehyde indole test to be species are LAP negative. to stain bacteria. performed in tubes, cupules, The disks are impregnated 15 tests ................ 10789-718 or on ID strips. with leucine-ß-naphthylamide, which can be hydrolyzed Legionella Latex 15ml, Each .......... 89407-610 by the enzyme leucine A rapid latex slide Indole, Rapid aminopeptidase produced by agglutination test for identifying Legionella pneumophila grown For the detection of indole some organisms. on selective medium. production by fastidious 3ml PYR reagent and LAP 50 tests per kit ...... microorganisms, especially disks, 25 disks ......10153-944 10032-744 Haemophilus spp., ™ Cardiobacterium spp., E. coliPRO O157, Cat. no. Suttonella spp., and other 89407-502, latex agglutination kit. fastidious bacteria. 5ml, 10/pk .......... 10324-572 Indole, DMACA Cinnamaldehyde reagent for performing rapid indole test on filter paper. 15ml, Each .......... 89428-932 Iodine, Dobell and O’Connor Iodine stain for parasite ova and cysts in fecal wet mounts. 15ml, Each .......... 10790-370 4 Rapid Tests & Reagents Mineral Oil Heavy mineral oil for biochemical tests that require oil overlays. 15ml, Each .......... 89407-734 Moraxella catarrhalis See CatScreen™ and CarboFerm™ MRSA Latex, PBP2’ A 15 minute latex agglutination test for EnteroScreen 4™, identification of PBP2’ in Cat. no. 10324-472. methicillin resistant Staphylococcus aureus. 50 tests ................ 10052-554 Listeria Latex MGP, Rapid (VRE) A latex agglutination slide Rapid enzymatic test to assist NALC test for the identification of in ID of vancomycin resistant (N-Acetyl-L-Cysteine) motile Listeria spp. from solid enterococci (VRE), rules out A digestant specimen selective media. non-pathogens Enterococcus preparation for AFB culture. 50 tests/kit ........... 10062-770 casseliflavus and Enterococcus For use with TB Digestant, sold gallinarum. separately. Lysostaphin 20 tubes/pk .........10324-570 20x250mg ........... 10324-432 A rapid test that differentiates between Micrococcus spp. Micrococcus Neisseria and Staphylococcus spp. See Lysostaphin. See CarboFerm™. 50 disks/vial ........10136-070 Mayer’s Albumin Used to adhere fecal Germ Tube Cryo™, specimens to glass slides in the Cat. no. 10324-568. Modified Iron Hematoxylin/ Kinyoun or Trichrome staining process. 15ml, Each .......... 10789-716 Methyl Red Developer reagent for the Methyl Red test. 15ml, Each .......... 10222-176 Methylene Blue, Loeffler’s General purpose bacterial stain, and for fecal leukocytes. 15ml, Each ..........10789-454 Rapid Tests & Reagents 5 Ninhydrin Color developer in alcohol for the hippurate hydrolysis test. 15ml, Each .......... 89407-768 Nitrate Reagent A (Sulfanilimide, 0.2%) Sulfanilic acid for nitrate reduction test, compatible with strip/cupule tests. 15ml, Each .......... 89407-776 Nitrate Reagent B (Naphthylethylenediamine, 0.1%) Naphthylamine for nitrate reduction test, compatible with strip/cupule tests. 15ml, Each .......... 89407-772 Positive Negative Uninoculated Nitrate Reagent C Indole Broth, Rapid, Cat. no. 10324-572. Zinc for nitrate reduction test. 5gm, Each ........... 89407-774 Novobiocin HardyDisks™, 5µg OxiDrops™ Oxidase For the identification of A sensitive, ready-to-use liquid ™ Nitrocef, HardyDisks Staphylococcus saprophyticus. reagent for the detection of A nitrocefin (chromogenic) 50 disks/vial ........ 10789-452 cytochrome oxidase activity. test for beta-lactamase activity 7ml, Each ............89428-918 in bacteria, disk format. Optochin, HardyDisks™ ™ 50 disks/vial ........ 10324-434 A disk for the differentiation of OxiStrips Oxidase alpha-hemolytic Streptococcus Paper strips, tetramethyl pneumoniae, (P
Recommended publications
  • Characterization and Antibiotic Sensitivity Profile of Bacteria Isolated from Patients with Respiratory Tract Infections in Bangladesh
    Characterization and Antibiotic Sensitivity Profile of Bacteria Isolated from Patients with Respiratory Tract Infections in Bangladesh Shukla Promite1, Sajal K. Saha2, Sunjukta Ahsan1 and Marufa Zerin Akhter1 1Department of Microbiology, University of Dhaka, Dhaka, Bangladesh 2Department of General Practice, Monash University, Building 1, 270 Ferntree Gully Road, Notting Hill VIC 3168, Australia (Received: October 08, 2017; Accepted: December 15, 2017; Published (web): December 23, 2017) ABSTRACT: The study was aimed to characterize bacterial isolates from respiratory tract infections (RTI) and investigate their antibiotic sensitivity profile. Selective media and biochemical tests were used to characterize 40 bacterial isolates. Antibiotic sensitivity testing was conducted using Kirby-Bauer disc diffusion method. About 42.5% (17) RTI patients were infected by Klebsiella pneumoniae, 30% (12) by Escherichia coli and 27.5% (11) by Pseudomonas aeruginosa with no significant gender variation (p-value <0.578). Overall, 47% (out of 20) antibiotics were sensitive, whereas 48% were resistant. Surprisingly, 18% P. aeruginosa and 20% K. pneumoniae were carbapenem-resistant and 4 out of 7 cephalosporin antibiotics were highly resistant irrespective of pathogens. E. coli showed better sensitivity to nitrofurantoin (78%) and levofloxacin (89%), while K. pneumoniae was insensitive to cotrimoxazole (88%), gentamycin (77%) and piperacillin/tazobactam (66%). On the other hand, P. aeruginosa did not respond to P. aeruginosa to nalidixic acid (60%) and ciprofloxacin (60%). This study concludes that nitrofurantoin, levofloxacin, cotrimoxazole, gentamycin and piperacillin/tazobactam antibiotics could be better alternative in treating bacterial RTIs. Key words: Antibiotic sensitivity, bacterial pathogens, RTIs, Bangladesh. INTRODUCTION Antibiotic resistance (AR) is a global public The rise of AR in Bangladesh is probably due to 1 health concern.
    [Show full text]
  • MIO Medium (Motility Indole Ornithine Medium) M378
    MIO Medium (Motility Indole Ornithine Medium) M378 Motility Indole Ornithine Medium (MIO Medium) is used for the identification of Enterobacteriaceae on the basis of motility, indole production and ornithine decarboxylase activity. Composition** Ingredients Gms / Litre Casein enzymic hydrolysate 10.000 Peptic digest of animal tissue 10.000 Yeast extract 3.000 L-Ornithine hydrochloride 5.000 Dextrose 1.000 Bromocresol purple 0.020 Agar 2.000 Final pH ( at 25°C) 6.5±0.2 **Formula adjusted, standardized to suit performance parameters Directions Suspend 31.02 grams in 1000 ml distilled water. Heat to boiling to dissolve the medium completely. Dispense in test tubes in 5 ml amounts. Sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes. Cool the tubes in an upright position. Principle And Interpretation Motility, indole production and ornithine decarboxylation are routine biochemical tests employed during identification of Enterobacteriaceae . Motility can be demonstrated microscopically (hanging drop) or macroscopically (tube method), where motility is observed as a diffused zone of growth flaring out from the line of inoculation. Indole test is carried out to determine the ability of an organism to split indole from tryptophan by the tryptophanase enzyme. On reaction with Kovacs reagent, indole combines with the colour in the alcohol layer, which is visualized as a red ring (in the alcohol layer) (1). If the test organisms possess the specific decarboxylase enzyme, then ornithine is decarboxylated to putrescine, an amine, resulting in a subsequent rise in the pH of the medium towards alkalinity. This causes the pH indicator bromocresol purple to change from purple to yellow colour.
    [Show full text]
  • 05686 DMACA (Indol Detection Disks)
    05686 DMACA (Indol Detection Disks) The DMACA Indole discs are used for Indole test to determine the ability of an organism to split tryptophan into indole and α-aminopropionic acid. The presence of indole can be detected by the addition of DMACA which results in a bluish-purple complex. With this method it is possible to differentiate Escherichia coli from Klebsiella. Composition: (1 package contains 50 disks) The discs contain p-Dimethylaminocinnamaldehyde (DMACA). Directions: Place the disc on suspected colony from e.g. UTI ChromoSelect Agar, modified (16636) or Christensen’s Urea Agar (27048) plate on filter paper. Observe for appearance of blue-purple color within 10-30 seconds. Quality control: Test Organisms (ATCC) DMACA Escherichia coli (25922) + Pseudomonas aeruginosa (27853) - Klebsiella pneumoniae (13883) - 1. Staphylococcus aureus 2. Escherichia coli Page 1 of 2 References: 1. R. Vracko, J.C. Sherris, Indole-spot test in bacteriology., Am. J. Clin. Pathol., 39, 429 (1963) 2. V.L. Sutter, W.T. Carter, Evaluation of media and reagents for indole-spot test in anaerobic bacteriology., Am. J. Clin. Pathol., 58, 335 (1972) 3. G.D. Fay, A.L. Barry, Methods for detecting indole production by gram-negative nonsporeforming anaerobes., Appl. Micro. 27, 562 (1974) 4. D.F. Welch, P.A. Ahlin, J.M. Matsen, Differentiation of Haemophilus spp. in respiratory isolate cultures by an indole spot test., J. Clin. Micro. 15, 216 (1982) 5. H.D. Isenberg, Ed., Clinical microbiology procedures handbook, Vol 1., Washington, DC, ASM (1992) 6. B.A. Forbes, D.F. Sahm, A.S. Weissfeld, Bailey and Scott's diagnostic microbiology., 10th ed., St Louis, Mosby (1998) 7.
    [Show full text]
  • Escherichia Coli Isolates Causing Asymptomatic Bacteriuria in Catheterized and Noncatheterized Individuals Possess Similar Virulence Propertiesᰔ 1 2 1 2
    JOURNAL OF CLINICAL MICROBIOLOGY, July 2010, p. 2449–2458 Vol. 48, No. 7 0095-1137/10/$12.00 doi:10.1128/JCM.01611-09 Copyright © 2010, American Society for Microbiology. All Rights Reserved. Escherichia coli Isolates Causing Asymptomatic Bacteriuria in Catheterized and Noncatheterized Individuals Possess Similar Virulence Propertiesᰔ 1 2 1 2 Rebecca E. Watts, Viktoria Hancock, Cheryl-Lynn Y. Ong, Rebecca Munk Vejborg, Downloaded from Amanda N. Mabbett,1 Makrina Totsika,1 David F. Looke,3 Graeme R. Nimmo,4 Per Klemm,2 and Mark A. Schembri1* School of Chemistry and Molecular Biosciences, University of Queensland, Brisbane, Queensland, Australia1; Microbial Adhesion Group, DTU Food, Technical University of Denmark, Lyngby, Denmark2; and Infection Management Services, Princess Alexandra Hospital,3 and Queensland Health Pathology Service, Department of Microbiology, Royal Brisbane and Women’s Hospital,4 Brisbane, Queensland, Australia Received 19 August 2009/Returned for modification 12 November 2009/Accepted 22 April 2010 http://jcm.asm.org/ Urinary tract infections (UTIs) are among the most common infectious diseases of humans, with Escherichia coli being responsible for >80% of all cases. Asymptomatic bacteriuria (ABU) occurs when bacteria colonize the urinary tract without causing clinical symptoms and can affect both catheterized patients (catheter- associated ABU [CA-ABU]) and noncatheterized patients. Here, we compared the virulence properties of a collection of ABU and CA-ABU nosocomial E. coli isolates in terms of antibiotic resistance, phylogenetic grouping, specific UTI-associated virulence genes, hemagglutination characteristics, and biofilm formation. CA-ABU isolates were similar to ABU isolates with regard to the majority of these characteristics; exceptions were that CA-ABU isolates had a higher prevalence of the polysaccharide capsule marker genes kpsMT II and on October 22, 2015 by University of Queensland Library kpsMT K1, while more ABU strains were capable of mannose-resistant hemagglutination.
    [Show full text]
  • Spot Indole Reagent
    PRODUCT DETERIORATION This product should not be used if (1) the color has changed, (2) the expiration date has passed, or (3) there are other signs of deterioration. SPOT INDOLE REAGENT SPECIMEN COLLECTION, STORAGE, TRANSPORT Specimens should be collected and handled INTENDED USE 3 Remel Spot Indole Reagent is recommended for use following recommended guidelines. in qualitative procedures to determine the ability of an organism to split indole from the tryptophan MATERIALS REQUIRED BUT NOT SUPPLIED molecule. (1) Loop sterilization device, (2) Inoculating loop, swab, collection containers, (3) Incubators, alternative SUMMARY AND EXPLANATION environmental systems, (4) Supplemental media, Vracko and Sherris, in 1963, utilized Spot Indole (5) Quality control organisms, (6) Whatman (No. 1) Reagent for the presumptive separation of the filter paper. Proteus species and Escherichia coli.1 In 1969, Lowrance, Reich, and Traub found ρ-Dimethylamino- PROCEDURE cinnamaldehyde to be the most sensitive indole Filter Paper Method: reagent, capable of detecting 3 mcg of indole per 1. Dispense 1 or 2 drops of reagent onto a piece of milliliter of medium.2 Whatman (No. 1) filter paper or equivalent. 2. Smear the growth from an actively growing pure PRINCIPLE culture onto the saturated filter paper. Intracellular enzymes (i.e., tryptophanases) mediate 3. Observe for the development of a blue color the production of indole by hydrolytic activity against within 1 to 3 minutes. the amino acid tryptophan. Indole combines with Swab Method: dimethylaminocinnamaldehyde to form a blue-green 1. Dispense 1 or 2 drops of reagent onto the tip of compound. The reaction occurs by a condensation a cotton swab.
    [Show full text]
  • Biochemical Tests Indole Test
    Biochemical Tests Indole test Objective: to detect the ability of organism to produce enzyme tryptophanase. Principle: Indole test is a biochemical test which differentiates the coliform from other members of Enterobacteriacee by detecting their ability to produce the enzyme tryptophanase. This enzyme hydrolyses the amino acid tryptophan into indole, pyruvic acid and ammonia. It is the intracellular enzyme (endoenzyme). Tryptophan + H2O————tryptophanase enzyme——> Indole + Pyruvic acid + Ammonia Pyruvic acid can then be used by the organism in the Kreb’s cycle or it can enter glycolysis and be used to synthesize other compounds necessary for the cell. The media that is used for indole test is SIM (sulphide, indole, motility) medium or nutrient peptone, both of those media provides sufficient amino acid, tryptophan which acts as substrate for the above reaction. Hence, the organism that are able to produce the pyruvic acid as main product and indole, ammonia as the byproduct. The reagent used for this test is kovac’s reagent; (HCI and dimenthyl aminobenzaldehyde dissolved in amyl alcohol) which reacts with the side product of the tryptophan catabolism reaction i.e indole to form Rosindole dye which is cherry red in colour. Hence the formation of cherry red colour of Rosindole dye indicates positive indole test, otherwise not Escherichia coli is positive to indole test while klebsiella is negative to it. Voges–Proskauer (VP) Test The Voges-Proskauer (VP) test is used to determine if an organism produces acetylmethyl carbinol from glucose fermentation. If present, acetylmethyl carbinol is converted to diacetyl in the presence of ∝- naphthol, strong alkali (40% KOH), and atmospheric oxygen.
    [Show full text]
  • SPOT INDOLE REAGENT Σ (For in Vitro Diagnostic Use) 250
    PRODUCT CODE PL.391-10 SPOT INDOLE REAGENT Σ (for in vitro diagnostic use) 250 INTENDED USE MATERIALS REQUIRED BUT NOT PROVIDED should be checked with known positive and negative control Pro-Lab’s Spot Indole Reagent is to be used in the qualitative method to 1. Inoculating loops organisms. determine the ability of an organism to split indole from the tryptophan 2. Filter paper (Whatman No. 1 or equivalent) 5. Only pure cultures of organisms are to be tested. Weakly false molecule. 3. Cotton-tipped swabs positive reactions may occur if the inoculum is a mixed culture of 4. Incubator indole positive and negative organisms, since adjacent colonies are SUMMARY AND EXPLANATION 5. Supplemental media likely to take up diffused indole4. Spot Indole Reagent was used by Vracko and Sherris in 1963 for the 6. Quality control organisms presumptive differentiation of Proteus species and Escherichia coli1. The REFERENCES work of Lowrence, Reich and Traub in 1969, indicated that p-diemethyl- PROCEDURE 1. Vracko, R. and J.C. Sherris. (1963). Am. J. Clin. Path. 39:429-432. aminocinnamaldeyde is the most sensitive indole reagent, capable of Allow the reagent to come to room temperature prior to use. 2. Lowrance, B.L., P. Reich and W.H. Traub. (1969). Appl. Microbiol. detecting 3 mcg of indole per millilitre of medium2. Filter Paper Method: 17:923-924. 1. Dispense 1 to 2 drops of Spot Indole Reagent onto a piece of filter 3. Balzevic, D.J. and G.M. Ederer. (1975). Principles of Biochemical Tests PRINCIPLE OF THE PROCEDURE paper (Whatman No.
    [Show full text]
  • Research Journal of Pharmaceutical, Biological and Chemical Sciences
    ISSN: 0975-8585 Research Journal of Pharmaceutical, Biological and Chemical Sciences Analysis of Important Dairy Products: Isolation and Characterization LK Attri* and Humeet Narang Desh Bhagat University, Mandi Gobindgarh-147 301, Punjab, India. ABSTRACT In the present study, different daily use dairy products were procured and isolation was carried out using standard morphological. Based upon the morphological and biochemical standard test, the isolates were confirmed to be E.coli, Pseudomonas, Lactobacillus and Staphylococcus. Different biochemical standard methods including Indole production test, Methyl-Red and Voges-Proskauer (MRVP) test, Catalase Test and Citrate Utilization Test etc. were performed for final conclusion. Therefore, the results showed the presence of harmful bacterias in daily use dairy product. Further, Slants of nutrient agar media were made and kept for solidification. After solidification pure colonies of isolates were streaked on these slants and incubated at 37° C for 2-3 days. After growth these slants were stored at 4° C for further use. Keywords: Antibacterial agents, bacterias, standard tests, nutrient medias *Corresponding author January - February 2014 RJPBCS 5(1) Page No. 698 ISSN: 0975-8585 INTRODUCTION Dairy products are generally defined as food produced from the milk of mammals (the Food Standards Agency of the United Kingdom defines dairy as "foodstuffs made from mammalian milk). They are usually high energy-yielding food products. A production plant for the processing of milk is called a dairy or a dairy factory. Apart from breastfed infants, the human consumption of dairy products is sourced primarily from the milk of cows, goats, sheep, yaks, camels, and other mammals are other sources of dairy products consumed by humans.
    [Show full text]
  • Indole Test Reagents ­ Kovacs, DMACA, Spot Test
    10/20/2016 Indole Test Reagents ­ Kovacs, DMACA, Spot test INDOLE TEST REAGENTS Cat. no. Z65 Indole Spot Reagent 15ml Cat. no. Z67 Indole Kovacs Reagent 15ml INTENDED USE Hardy Diagnostics Indole Spot Reagent and Indole Kovacs Reagent are recommended for use in determining the indole reaction of bacteria. SUMMARY The indole test is a qualitative procedure for determining the ability of bacteria to produce indole by deamination of tryptophan. Using Kovacs tube method, indole combines, in the presence of a tryptophan rich medium, with p­ Dimethylaminobenzaldehyde at an acid pH in alcohol to produce a red­violet compound. In the spot test, indole combines, in the filter paper matrix, at an acid pH with p­Dimethylaminocinnamaldehyde (DMACA) to produce a blue to blue­green compound. Indole Spot Reagent (DMACA) has been reported to be useful in detecting indole production by members of the family Enterobacteriaceae and certain anaerobic species. REAGENT FORMULA Ingredients per liter:* Indole Spot Reagent: p­Dimethylaminocinnamaldehyde (DMACA) 10.0gm Hydrochloric Acid, 37% 100.0ml Deionized Water 900.0ml Indole Kovacs Reagent: p­Dimethylaminobenzaldehyde 50.0gm Hydrochloric Acid, 37% 250.0ml Amyl Alcohol 750.0ml * Adjusted and/or supplemented as required to meet performance criteria. STORAGE AND SHELF LIFE https://catalog.hardydiagnostics.com/cp_prod/Content/hugo/IndoleTestRgnts.htm 1/5 10/20/2016 Indole Test Reagents ­ Kovacs, DMACA, Spot test Storage: Upon receipt store at 2­30ºC. Products should not be used if there are any signs of deterioration or if the expiration date has passed. The expiration dating on the product label applies to the product in its intact packaging when stored as directed.
    [Show full text]
  • Practicalities of Maldi-Tof*
    PRACTICALITIES OF MALDI-TOF* REPORTING, QC/TROUBLESHOOTING, FURTHER APPLICATIONS (AND OTHER MISCELLANY) DR DAN LENNON, MICROBIOLOGY REGISTRAR, NSWHP-HUNTER, NSW AUSTRALIA JUNE 2020 DISCLOSURES No conflicts of interest to disclose: no affiliation with any company As my lab uses Bruker, will focus on this platform, I will focus on this platform. Clark AE, Kaleta EJ, Arora A, Wolk DM. Matrix-Assisted Laser Desorption Ionization–Time of Flight Mass Spectrometry: a Fundamental Shift in the Routine Practice of Clinical Microbiology. Clinical Microbiology Reviews. 2013;26(3):547-603. QUICK REVISION (FOR THOSE WHO MISSED TONY'S TALK) SLIDES Biotyper has two types of target slides disposable, single use only slides (MBT Biotarget 96) Reusable, ground steel target plates with hydrophobic target rings Laser requires an extremely flat surface – target plate will become unusable if dropped or bent Needs to be thoroughly washed after use to prevent memory effects SAMPLE PREPARATION SAMPLE PREPARATION REPORTING Score value: log score that represents the probability of isolate ID SV is a composite score of 3 values Number of peaks in the reference spectrum that have a closely matching partner in the test spectrum ( value 0-1) Number of peaks in the test spectrum that have a closely matching partner in the reference spectrum (value 0-1) Peak height symmetry of the matching peaks (value 0 – 1) Above 3 values are multiplied together and normalised to 1000, and then the base 10 logarithm is taken to give a final Bruker score Michael A Reeve,
    [Show full text]
  • Indole Test Protocol
    Indole Test Protocol | | Created: Tuesday, 08 December 2009 Author • Maria P. MacWilliams Information History As far back as 1889, the indole test was used as a means to distinguish between Escherichia coli andEnterobacter aerogenes (4). The numerous variations of the indole test alone and in combination with other biochemical tests attest to the central role this test has played in the characterization of coliforms (gram-negative nonsporulating bacilli that ferment lactose, producing acid and gas) (5). The indole test is still used as a classic test to distinguish indole-positive E. coli from indole- negativeEnterobacter and Klebsiella. (8) Purpose The indole test screens for the ability of an organism to degrade the amino acid tryptophan and produce indole. It is used as part of the IMViC procedures, a battery of tests designed to distinguish among members of the family Enterobacteriaceae. Theory (3, 5) Tryptophan is an amino acid that can undergo deamination and hydrolysis by bacteria that express tryptophanase enzyme. tryptophan + water = indole + pyruvic acid + ammonia The chief requirement for culturing an organism prior to performing the indole test is that the medium contains a sufficient quantity of tryptophan (5). The presence of indole when a microbe is grown in a medium rich in tryptophan demonstrates that an organism has the capacity to degrade tryptophan. Detection of indole, a by-product of tryptophan metabolism, relies upon the chemical reaction between indole and p- dimethylaminobenzaldehyde (DMAB) under acidic conditions to produce the red dye rosindole (5, 8). RECIPES The main requirement for a suitable indole test medium is that it contain a sufficient amount of tryptophan.
    [Show full text]
  • INDOLE SPOT REAGENT (1% CINNAMALDEHYDE) - for in Vitro Use Only - Catalogue No
    INDOLE SPOT REAGENT (1% CINNAMALDEHYDE) - For in vitro use only - Catalogue No. RI40 Our Indole Spot Reagent is used to detect the 3. Proteus vulgaris (+), P. inconstans (+), P. presence of indole, which is one of the degradation rettgeri (+) from other Proteus spp. (-) products of the bacterial metabolism of 4. Klebsiella oxytoca (+), K. ornithinolytica (+) tryptophan. from other Klebsiella spp. (usually -) Tryptophan is an amino acid that can be Other uses: oxidized by some bacteria to form three major end 1. Along with other tests (urease and ornithine) products: indole, pyruvic acid, and ammonia. subdivides Haemophilus influenzae and Detection of indole indicates tryptophan Haemophilus parainfluenzae into biotypes degradation and can be accomplished by the 2. Along with sialidase, α and β-glucosidase, addition of certain aldehydes to form colored end α-fructosidase, differentiates between products. Indole Reagent contains the active black-pigmented anaerobes: Porphyromonas ingredient, p-dimethylaminocinnamaldehyde asaccharolyticus (+), P. endodontalis (+), P. (PACA), which reacts with indole to form a gingivalis (+), and Prevotella intermedia (+) blue-green compound. Other available reagents from Prevetolla corporis (-), P. denticola (-), for the detection of indole include Ehrlich’s P. loesheii (-), P. melaninogenica (-), and reagent and Kovac’s Reagent (Dalynn RK75) both Porphyromonas levii (-) of which contain the aldehyde p-dimethylamino-benzaldehyde. Comparatively, Indole Reagent (PACA) is the most sensitive Formulation per 100 mL reagent and can detect as little as 3.0 µg of indole per mL. p-Dimethylaminocinnamaldehyde ................. 1.0 g Indole Reagent is the reagent of choice for Hydrochloric Acid (10%) ...................... 100.0 mL performing the spot indole test. The spot indole test is a rapid procedure designed by Vracko and Sherris.
    [Show full text]