Association of DNA with Nuclear Matrix in in Vitro Assembled Nuclei
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General Information on Logistics for the Second International Symposium on Nuclear Power Plant Life Management Shanghai, China, 15-18 October 2007 ______
General Information on Logistics for the Second International Symposium on Nuclear Power Plant Life Management Shanghai, China, 15-18 October 2007 __________________________________________________________________________________ Symposium Venue: Shanghai International Convention Center (Oriental Riverside Hotel) 2727, Riverside Avenue Shanghai 200120 China Tel: +86 21 50370000 Fax : +86 21 50370045 Email: [email protected] Web site: www.shicc.net The Shanghai International Convention Center is located in the heart of Lujia Zui - Shanghai's Financial & Trade Zone and southwest of the Oriental Pearl Tower. See map on this web site. Registration: The Registration Desk at the Shanghai International Convention Center (7th floor in front of the Grand Ballroom) will be open on Sunday, 14 October 2007 from 16:00 to 18:00 hours and on Monday, 15 October from 8:00 hours onwards. Registration is also possible throughout the week during the working hours of the symposium. Participants are requested, if possible, to register on Sunday, 14 October 2007. No registration fee is charged. Opening of the Symposium: Monday, 15 October 2007, at 09:00 hours. Documentation: A preliminary programme is available on the symposium web site. The final programme and the Book of Extended Synopses will be available upon registration. Proceedings: The proceedings of the symposium will be published by the IAEA as soon as possible after the meeting. Accommodation: Participants are expected to make their own travel and hotel arrangements. Block reservations have been made in the following three hotels: Oriental Riverside Hotel (Shanghai International Convention Center), Tong Mao Hotel, Embassy Suite Hotel. It has been possible to extend the deadline from 30 June to 31 July. -
This Is a Sample Copy, Not to Be Reproduced Or Sold
Startup Business Chinese: An Introductory Course for Professionals Textbook By Jane C. M. Kuo Cheng & Tsui Company, 2006 8.5 x 11, 390 pp. Paperback ISBN: 0887274749 Price: TBA THIS IS A SAMPLE COPY, NOT TO BE REPRODUCED OR SOLD This sample includes: Table of Contents; Preface; Introduction; Chapters 2 and 7 Please see Table of Contents for a listing of this book’s complete content. Please note that these pages are, as given, still in draft form, and are not meant to exactly reflect the final product. PUBLICATION DATE: September 2006 Workbook and audio CDs will also be available for this series. Samples of the Workbook will be available in August 2006. To purchase a copy of this book, please visit www.cheng-tsui.com. To request an exam copy of this book, please write [email protected]. Contents Tables and Figures xi Preface xiii Acknowledgments xv Introduction to the Chinese Language xvi Introduction to Numbers in Chinese xl Useful Expressions xlii List of Abbreviations xliv Unit 1 问好 Wènhǎo Greetings 1 Unit 1.1 Exchanging Names 2 Unit 1.2 Exchanging Greetings 11 Unit 2 介绍 Jièshào Introductions 23 Unit 2.1 Meeting the Company Manager 24 Unit 2.2 Getting to Know the Company Staff 34 Unit 3 家庭 Jiātíng Family 49 Unit 3.1 Marital Status and Family 50 Unit 3.2 Family Members and Relatives 64 Unit 4 公司 Gōngsī The Company 71 Unit 4.1 Company Type 72 Unit 4.2 Company Size 79 Unit 5 询问 Xúnwèn Inquiries 89 Unit 5.1 Inquiring about Someone’s Whereabouts 90 Unit 5.2 Inquiring after Someone’s Profession 101 Startup Business Chinese vii Unit -
PSF and P54nrb/Nono ^ Multi-Functional Nuclear Proteins
FEBS 26628 FEBS Letters 531 (2002) 109^114 View metadata, citation and similar papers at core.ac.uk brought to you by CORE Minireview provided by Elsevier - Publisher Connector PSF and p54nrb/NonO ^ multi-functional nuclear proteins Yaron Shav-Tal, Dov Ziporià Department of Molecular Cell Biology, The Weizmann Institute of Science, Rehovot 76100, Israel Received 30 May 2002; revised 10 September 2002; accepted 11 September 2002 First published online 7 October 2002 Edited by Takashi Gojobori glutamine-rich N-terminus of PSF might be involved in pro- Abstract Proteins are often referred to in accordance with the activity with which they were ¢rst associated or the organelle in tein^protein interactions [2]. nrb which they were initially identi¢ed. However, a variety of nu- p54 (human) and NonO (mouse) are highly homologous clear factors act in multiple molecular reactions occurring si- to the C-terminus of PSF (Fig. 1) [7,8]. Proteomics have iden- multaneously within the nucleus. This review describes the func- ti¢ed PSF and p54nrb/NonO in the nucleolus [9] and in asso- tions of the nuclear factors PSF (polypyrimidine tract-binding ciation with the nuclear membrane [10]. p54nrb/NonO was protein-associated splicing factor) and p54nrb/NonO. PSF was recently shown to be a component of a novel nuclear domain initially termed a splicing factor due to its association with the termed paraspeckles [11].TheDrosophila homolog of these nrb second step of pre-mRNA splicing while p54 /NonO was proteins is the NONA/BJ6 protein encoded by the no-on-tran- thought to participate in transcriptional regulation. -
Narrative Inquiry Into Chinese International Doctoral Students
Volume 16, 2021 NARRATIVE INQUIRY INTO CHINESE INTERNATIONAL DOCTORAL STUDENTS’ JOURNEY: A STRENGTH-BASED PERSPECTIVE Shihua Brazill Montana State University, Bozeman, [email protected] MT, USA ABSTRACT Aim/Purpose This narrative inquiry study uses a strength-based approach to study the cross- cultural socialization journey of Chinese international doctoral students at a U.S. Land Grant university. Historically, we thought of socialization as an institu- tional or group-defined process, but “journey” taps into a rich narrative tradi- tion about individuals, how they relate to others, and the identities that they carry and develop. Background To date, research has employed a deficit perspective to study how Chinese stu- dents must adapt to their new environment. Instead, my original contribution is using narrative inquiry study to explore cross-cultural socialization and mentor- ing practices that are consonant with the cultural capital that Chinese interna- tional doctoral students bring with them. Methodology This qualitative research uses narrative inquiry to capture and understand the experiences of three Chinese international doctoral students at a Land Grant in- stitute in the U.S. Contribution This study will be especially important for administrators and faculty striving to create more diverse, supportive, and inclusive academic environments to en- hance Chinese international doctoral students’ experiences in the U.S. Moreo- ver, this study fills a gap in existing research by using a strength-based lens to provide valuable practical insights for researchers, practitioners, and policymak- ers to support the unique cross-cultural socialization of Chinese international doctoral students. Findings Using multiple conversational interviews, artifacts, and vignettes, the study sought to understand the doctoral experience of Chinese international students’ experience at an American Land Grant University. -
Kūnqǔ in Practice: a Case Study
KŪNQǓ IN PRACTICE: A CASE STUDY A DISSERTATION SUBMITTED TO THE GRADUATE DIVISION OF THE UNIVERSITY OF HAWAI‘I AT MĀNOA IN PARTIAL FULFILLMENT OF THE REQUIREMENTS FOR THE DEGREE OF DOCTOR OF PHILOSOPHY IN THEATRE OCTOBER 2019 By Ju-Hua Wei Dissertation Committee: Elizabeth A. Wichmann-Walczak, Chairperson Lurana Donnels O’Malley Kirstin A. Pauka Cathryn H. Clayton Shana J. Brown Keywords: kunqu, kunju, opera, performance, text, music, creation, practice, Wei Liangfu © 2019, Ju-Hua Wei ii ACKNOWLEDGEMENTS I wish to express my gratitude to the individuals who helped me in completion of my dissertation and on my journey of exploring the world of theatre and music: Shén Fúqìng 沈福庆 (1933-2013), for being a thoughtful teacher and a father figure. He taught me the spirit of jīngjù and demonstrated the ultimate fine art of jīngjù music and singing. He was an inspiration to all of us who learned from him. And to his spouse, Zhāng Qìnglán 张庆兰, for her motherly love during my jīngjù research in Nánjīng 南京. Sūn Jiàn’ān 孙建安, for being a great mentor to me, bringing me along on all occasions, introducing me to the production team which initiated the project for my dissertation, attending the kūnqǔ performances in which he was involved, meeting his kūnqǔ expert friends, listening to his music lessons, and more; anything which he thought might benefit my understanding of all aspects of kūnqǔ. I am grateful for all his support and his profound knowledge of kūnqǔ music composition. Wichmann-Walczak, Elizabeth, for her years of endeavor producing jīngjù productions in the US. -
Localization of Condensin Subunit XCAP-E in Interphase Nucleus, Nucleoid and Nuclear
1 Localization of condensin subunit XCAP-E in interphase nucleus, nucleoid and nuclear matrix of XL2 cells. Elmira Timirbulatova, Igor Kireev, Vladimir Ju. Polyakov, and Rustem Uzbekov* Division of Electron Microscopy, A.N.Belozersky Institute of Physico-Chemical Biology, Moscow State University, 119899, Moscow, Russia. *Author for correspondence: telephone. 007-095-939-55-28; FAX 007-095-939-31-81 e-mail: [email protected] Key words: XCAP-E; nucleolus; condensin; nuclear matrix; Xenopus. Abbreviations: DAPI , 4’, 6 diamidino-2-phenylindole; DNP, deoxyribonucleoprotein; DRB, 5,6-dichloro-1b-d-ribofuranosylbenzimidazole; SMC, structural maintenance of chromosomes; XCAP-E, Xenopus chromosome associated protein E. 2 Abstract The Xenopus XCAP-E protein is a component of condensin complex In the present work we investigate its localization in interphase XL2 cells and nucleoids. We shown, that XCAP-E is localizes in granular and in dense fibrillar component of nucleolus and also in small karyoplasmic structures (termed “SMC bodies”). Extraction by 2M NaCl does not influence XCAP-E distribution in nucleolus and “SMC bodies”. DNAse I treatment of interphase cells permeabilized by Triton X-100 or nucleoids resulted in partial decrease of labeling intensity in the nucleus, whereas RNAse A treatment resulted in practically complete loss of labeling of nucleolus and “SMC bodies” labeling. In mitotic cells, however, 2M NaCl extraction results in an intense staining of the chromosome region although the labeling was visible along the whole length of sister chromatids, with a stronger staining in centromore region. The data are discussed in view of a hypothesis about participation of XCAP-E in processing of ribosomal RNA. -
Disease and Clinical Signs Poster
African Clawed Frog Xenopus laevis Diseases and Clinical Signs in Photographs Therese Jones-Green University Biomedical Services Abstract Reported clinical signs associated with ill health and Clinical signs found between 2016 to 2018 Many diseases of Xenopus laevis frogs have been described in the • Opened in 2005, the biofacility can hold up to 900 female and 240 disease Clinical signs found between 2016 to 2018 male frogs in a Marine Biotech (now serviced by MBK Installations Ltd.) • 80 recirculating aquatic system. • Buoyancy problems 70 bridge this gap using photographs taken of frogs with clinical signs. The system monitors parameters such as: temperature, pH, • • Changes in activity e.g. lethargy or easily startled. My aims are: conductivity, and Total Gas Pressure. 60 • Unusual amount of skin shedding. 2018 • To share my experiences, via images, to help improve the welfare and • Frogs are maintained on mains fed water with a 20% water exchange. 50 ases • Weight loss, or failure to feed correctly. c husbandry of this species. • 2017 • Coelomic (body cavity) distention. 40 • To stimulate a dialogue which results in further sharing of ideas and 2016 Whole body bloat. 30 information. • Frogs are held under a 12 hour light/dark cycle. • Number of • Each tank has a PVC enrichment tube (30cm x 10cm), with smoothed • Fungal strands/tufts. 20 Introduction cut edges. • Sores or tremors at extremities (forearms and toes). 10 Between January 2016 and October 2018, cases of ill health and disease • Frogs are fed three times a week with a commercial trout pellet. • Redness or red spots. 0 Oedema Red leg Red leg (bad Red leg Gas bubble Sores on forearm Opaque cornea Skeletal Growth (cartilage Scar Cabletie Missing claws Eggbound in a colony of Xenopus laevis frogs housed by the University Biological hormone) (nematodes) disease deformity under skin) • The focus of the research undertaken in the biofacility is embryonic and • Excessive slime/mucous production or not enough (dry dull skin). -
Building the Interphase Nucleus: a Study on the Kinetics of 3D Chromosome Formation, Temporal Relation to Active Transcription, and the Role of Nuclear Rnas
University of Massachusetts Medical School eScholarship@UMMS GSBS Dissertations and Theses Graduate School of Biomedical Sciences 2020-07-28 Building the Interphase Nucleus: A study on the kinetics of 3D chromosome formation, temporal relation to active transcription, and the role of nuclear RNAs Kristin N. Abramo University of Massachusetts Medical School Let us know how access to this document benefits ou.y Follow this and additional works at: https://escholarship.umassmed.edu/gsbs_diss Part of the Bioinformatics Commons, Cell Biology Commons, Computational Biology Commons, Genomics Commons, Laboratory and Basic Science Research Commons, Molecular Biology Commons, Molecular Genetics Commons, and the Systems Biology Commons Repository Citation Abramo KN. (2020). Building the Interphase Nucleus: A study on the kinetics of 3D chromosome formation, temporal relation to active transcription, and the role of nuclear RNAs. GSBS Dissertations and Theses. https://doi.org/10.13028/a9gd-gw44. Retrieved from https://escholarship.umassmed.edu/ gsbs_diss/1099 Creative Commons License This work is licensed under a Creative Commons Attribution-Noncommercial 4.0 License This material is brought to you by eScholarship@UMMS. It has been accepted for inclusion in GSBS Dissertations and Theses by an authorized administrator of eScholarship@UMMS. For more information, please contact [email protected]. BUILDING THE INTERPHASE NUCLEUS: A STUDY ON THE KINETICS OF 3D CHROMOSOME FORMATION, TEMPORAL RELATION TO ACTIVE TRANSCRIPTION, AND THE ROLE OF NUCLEAR RNAS A Dissertation Presented By KRISTIN N. ABRAMO Submitted to the Faculty of the University of Massachusetts Graduate School of Biomedical Sciences, Worcester in partial fulfillment of the requirements for the degree of DOCTOR OF PHILOSPOPHY July 28, 2020 Program in Systems Biology, Interdisciplinary Graduate Program BUILDING THE INTERPHASE NUCLEUS: A STUDY ON THE KINETICS OF 3D CHROMOSOME FORMATION, TEMPORAL RELATION TO ACTIVE TRANSCRIPTION, AND THE ROLE OF NUCLEAR RNAS A Dissertation Presented By KRISTIN N. -
Nuclear Matrix
Nuclear matrix, nuclear envelope and premature aging syndromes in a translational research perspective Pierre Cau, Claire Navarro, Karim Harhouri, Patrice Roll, Sabine Sigaudy, Elise Kaspi, Sophie Perrin, Annachiara de Sandre-Giovannoli, Nicolas Lévy To cite this version: Pierre Cau, Claire Navarro, Karim Harhouri, Patrice Roll, Sabine Sigaudy, et al.. Nuclear matrix, nuclear envelope and premature aging syndromes in a translational research perspective. Seminars in Cell and Developmental Biology, Elsevier, 2014, 29, pp.125-147. 10.1016/j.semcdb.2014.03.021. hal-01646524 HAL Id: hal-01646524 https://hal-amu.archives-ouvertes.fr/hal-01646524 Submitted on 20 Dec 2017 HAL is a multi-disciplinary open access L’archive ouverte pluridisciplinaire HAL, est archive for the deposit and dissemination of sci- destinée au dépôt et à la diffusion de documents entific research documents, whether they are pub- scientifiques de niveau recherche, publiés ou non, lished or not. The documents may come from émanant des établissements d’enseignement et de teaching and research institutions in France or recherche français ou étrangers, des laboratoires abroad, or from public or private research centers. publics ou privés. Review Nuclear matrix, nuclear envelope and premature aging syndromes in a translational research perspective Pierre Cau a,b,c,∗, Claire Navarro a,b,1, Karim Harhouri a,b,1, Patrice Roll a,b,c,1,2, Sabine Sigaudy a,b,d,1,3, Elise Kaspi a,b,c,1,2, Sophie Perrin a,b,1, Annachiara De Sandre-Giovannoli a,b,d,1,3, Nicolas Lévy a,b,d,∗∗ a Aix-Marseille -
HIV-1 Transmitted Drug Resistance-Associated Mutations
Jiao et al. BMC Infectious Diseases 2014, 14:689 http://www.biomedcentral.com/1471-2334/14/689 RESEARCH ARTICLE Open Access HIV-1 transmitted drug resistance-associated mutations and mutation co-variation in HIV-1 treatment-naïve MSM from 2011 to 2013 in Beijing, China Yang Jiao1,2†, Shuming Li2†, Zhenpeng Li1, Zheng Zhang2, Jianhong Zhao2,LiLi2, Lijuan Wang2, Qianqian Yin1, Yan Wang1, Zhaoli Zeng2, Yiming Shao1* and Liying Ma1* Abstract Background: Transmitted drug resistance (TDR) is an important public health issue, because TDR-associated mutation may affect the outcome of antiretroviral treatment potentially or directly. Men who have sex with men (MSM) constitute a major risk group for HIV transmission. However, current reports are scarce on HIV TDR-associated mutations and their co-variation among MSM. Methods: Blood samples from 262 newly diagnosed HIV-positive, antiretroviral therapy (ART)-naïve MSM, were collected from January 2011 and December 2013 in Beijing. The polymerase viral genes were sequenced to explore TDR-associated mutations and mutation co-variation. Results: A total of 223 samples were sequenced and analyzed. Among them, HIV-1 CRF01_AE are accounted for 60.5%, followed by CRF07_BC (27.8%), subtype B (9.9%), and others. Fifty-seven samples had at least one TDR-associated mutation, mainly including L10I/V (6.3%), A71L/T/V (6.3%), V179D/E (5.4%), and V106I (2.7%), with different distributions of TDR-associated mutations by different HIV-1 subtypes and by each year. Moreover, eight significant co-variation pairs were found between TDR-associated mutations (V179D/E) and seven overlapping polymorphisms in subtype CRF01_AE. -
Xenopus for Dummies.Docx
Xenopus for DUMMIES 1 Xenopus for DUMMIES OUTLINE Introduction ........................................................................................................................... 2 1. Why is Xenopus interesting for iGEM? ........................................................................... 4 2. Requirements before planning to work on Xenopus......................................................... 5 3. Guidelines to define an iGEM project with Xenopus ........................................................ 5 4. The micro-injection procedure ........................................................................................ 7 a. Step 1: Fertilized eggs ........................................................................................ 7 b. Step 2: DNA injection .......................................................................................... 7 c. Place the injected embryos in incubator 21°C ..................................................... 8 d. The following days .............................................................................................. 8 e. Embryos injection: efficiency ............................................................................... 8 5. The biobrick plasmids for Xenopus ................................................................................. 9 6. Xenopus debugging tool ............................................................................................... 10 a. How? ................................................................................................................ -
Nuclear Domains
View metadata, citation and similar papers at core.ac.uk brought to you by CORE provided by Cold Spring Harbor Laboratory Institutional Repository CELL SCIENCE AT A GLANCE 2891 Nuclear domains dynamic structures and, in addition, nuclear pore complex has been shown to rapid protein exchange occurs between have a remarkable substructure, in which David L. Spector many of the domains and the a basket extends into the nucleoplasm. Cold Spring Harbor Laboratory, One Bungtown nucleoplasm (Misteli, 2001). An The peripheral nuclear lamina lies Road, Cold Spring Harbor, NY 11724, USA extensive effort is currently underway by inside the nuclear envelope and is (e-mail: [email protected]) numerous laboratories to determine the composed of lamins A/C and B and is biological function(s) associated with thought to play a role in regulating Journal of Cell Science 114, 2891-2893 (2001) © The Company of Biologists Ltd each domain. The accompanying poster nuclear envelope structure and presents an overview of commonly anchoring interphase chromatin at the The mammalian cell nucleus is a observed nuclear domains. nuclear periphery. Internal patches of membrane-bound organelle that contains lamin protein are also present in the the machinery essential for gene The nucleus is bounded by a nuclear nucleoplasm (Moir et al., 2000). The expression. Although early studies envelope, a double-membrane structure, cartoon depicts much of the nuclear suggested that little organization exists of which the outer membrane is envelope/peripheral lamina as within this compartment, more contiguous with the rough endoplasmic transparent, so that internal structures contemporary studies have identified an reticulum and is often studded with can be more easily observed.