DOCSLIB.ORG

Actinopolyspora Saharensis Sp. Nov., a Novel Halophilic Actinomycete Isolated from a Saharan Soil of Algeria

Total Page：16

File Type：pdf, Size：1020Kb

Download full-text PDF Read full-text
Actinopolyspora Saharensis Sp. Nov., a Novel Halophilic Actinomycete Isolated from a Saharan Soil of Algeria See discussions, stats, and author profiles for this publication at: https://www.researchgate.net/publication/236639031 Saccharothrix saharensis sp nov., an actinomycete isolated from Algerian Saharan soil Article in International Journal of Systematic and Evolutionary Microbiology · May 2013 DOI: 10.1099/ijs.0.051839-0 · Source: PubMed CITATIONS READS 17 182 9 authors, including: Abdelghani Zitouni Noureddine Bouras École Normale Supérieure de Kouba, Alger, Algeria Université de Ghardaia 155 PUBLICATIONS 1,712 CITATIONS 219 PUBLICATIONS 1,072 CITATIONS SEE PROFILE SEE PROFILE Florence Mathieu Ahmed Lebrihi Institut National Polytechnique de Toulouse Institut National Polytechnique de Toulouse 265 PUBLICATIONS 4,922 CITATIONS 213 PUBLICATIONS 5,395 CITATIONS SEE PROFILE SEE PROFILE Some of the authors of this publication are also working on these related projects: Evaluation of microbial diversity of Gomishan wetland in Iran View project Ressources génétiques sahariennes View project All content following this page was uploaded by Ahmed Lebrihi on 29 January 2015. The user has requested enhancement of the downloaded file. Actinopolyspora saharensis sp. nov., a novel halophilic actinomycete isolated from a Saharan soil of Algeria Atika Meklat, Noureddine Bouras, Abdelghani Zitouni, Florence Mathieu, Ahmed Lebrihi, Peter Schumann, Cathrin Spröer, et al. Antonie van Leeuwenhoek Journal of Microbiology ISSN 0003-6072 Volume 103 Number 4 Antonie van Leeuwenhoek (2013) 103:771-776 DOI 10.1007/s10482-012-9859-z 1 23 Your article is protected by copyright and all rights are held exclusively by Springer Science +Business Media Dordrecht. This e-offprint is for personal use only and shall not be self- archived in electronic repositories. If you wish to self-archive your work, please use the accepted author’s version for posting to your own website or your institution’s repository. You may further deposit the accepted author’s version on a funder’s repository at a funder’s request, provided it is not made publicly available until 12 months after publication. 1 23 Author's personal copy Antonie van Leeuwenhoek (2013) 103:771–776 DOI 10.1007/s10482-012-9859-z ORIGINAL PAPER Actinopolyspora saharensis sp. nov., a novel halophilic actinomycete isolated from a Saharan soil of Algeria Atika Meklat • Noureddine Bouras • Abdelghani Zitouni • Florence Mathieu • Ahmed Lebrihi • Peter Schumann • Cathrin Spro¨er • Hans-Peter Klenk • Nasserdine Sabaou Received: 3 August 2012 / Accepted: 20 November 2012 / Published online: 30 November 2012 Ó Springer Science+Business Media Dordrecht 2012 Abstract A novel halophilic actinomycete, strain 6.0–7.0 and in the presence of 15–25 % (w/v) NaCl. The H32T, was isolated from a Saharan soil sample collected strain was observed to produce abundant aerial myce- in El-Oued province, south Algeria. The isolate was lium, which formed long chains of rod-shaped spores at characterized by means of polyphasic taxonomy. Opti- maturity, and fragmented substrate mycelium. The cell mal growth was determined to occur at 28–32 °C, pH wall was determined to contain meso-diaminopimelic acid and the characteristic whole-cell sugars were arabinose and galactose. The predominant menaqui- nones were found to be MK-10(H4) and MK-9(H4). The Electronic supplementary material The online version of predominant cellular fatty acids were determined to be this article (doi:10.1007/s10482-012-9859-z) contains supplementary material, which is available to authorized users. anteiso C17:0,iso-C15:0 and iso-C16:0. The diagnostic phospholipid detected was phosphatidylcholine. Phylo- A. Meklat Á N. Bouras Á A. Zitouni Á N. Sabaou (&) genetic analyses based on the 16S rRNA gene sequence Laboratoire de Biologie des Syste`mes Microbiens showed that this strain formed a distinct phyletic line (LBSM), Ecole Normale Supe´rieure de Kouba, Alger, Algeria within the radiation of the genus Actinopolyspora.The e-mail: [email protected] 16S rRNA gene sequence similarity indicated that strain H32T was most closely related to ‘Actinopolyspora A. Meklat algeriensis’ DSM 45476T (98.8 %) and Actinopolys- De´partement de Biologie, Faculte´ des sciences T Agronomiques, Ve´te´rinaires et Biologiques, pora halophila DSM 43834 (98.5 %). Furthermore, Universite´ Saaˆd Dahleb de Blida, Blida, Algeria the result of DNA–DNA hybridization between strain H32T and the type strains ‘A. algeriensis’ DSM 45476T, F. Mathieu Á A. Lebrihi A. halophila DSM 43834T and Actinopolyspora morti- Laboratoire de Ge´nie Chimique, UMR 5503 T (CNRS/INPT/UPS), INPT-ENSAT, Universite´ de vallis DSM 44261 demonstrated that this isolate Toulouse, 1 Avenue de l’Agrobiopoˆle, Auzeville represents a different genomic species in the genus Tolosane, BP 32607, 31326 Castanet-Tolosan, France Actinopolyspora. Moreover, the physiological and biochemical data allowed the differentiation of strain A. Lebrihi T Universite´ Moulay Ismail, Marjane 2, BP 298 Meknes, H32 from its closest phylogenetic neighbours. There- T Morocco fore, it is proposed that strain H32 represents a novel species of the genus Actinopolyspora, for which the P. Schumann Á C. Spro¨er Á H.-P. Klenk name Actinopolyspora saharensis sp. nov. is proposed. Leibniz Institute DSMZ, German Collection T T of Microorganisms and Cell Cultures, Inhoffenstraße 7B, ThetypestrainisH32 (=DSM 45459 =CCUG T 38124 Braunschweig, Germany 62966 ). 123 Author's personal copy 772 Antonie van Leeuwenhoek (2013) 103:771–776 Keywords Actinopolyspora Á A. saharensis sp. nov. Á using humic acid-vitamin agar medium (Hayakawa Halophilic actinomycete Á Sahara Á and Nonomura 1987) supplemented with actidione Polyphasic taxonomy (50 lgml-1) and 20 % (w/v) NaCl. After 4 weeks of incubation at 30 °C, the isolate, which formed a white colony, was transferred and purified on complex Introduction medium (CM) agar (Chun et al. 2000) supplemented with 20 % (w/v) NaCl. The purified strain was The genus Actinopolyspora wasproposedbyGochnauer maintained on CM agar slants at 4 °C and as 20 % et al. (1975) and its description has been emended (v/v) glycerol suspensions at -20 °C. Strain H32T was recently by Tang et al. (2011). It currently encom- deposited in the German Collection of Microorgan- passes the species Actinopolyspora halophila isms and Cell Cultures (DSMZ) as strain DSM (Gochnauer et al. 1975), Actinopolyspora mortivallis 45459T, and in the Culture Collection, University of (Yoshida et al. 1991), Actinopolyspora xinjiangensis Go¨teborg, Sweden (CCUG) as strain CCUG 62966T. (Guan et al. 2010), Actinopolyspora egyptensis (Hozzein and Goodfellow 2011), Actinopolyspora Phenotypic characterization alba and Actinopolyspora erythraea (Tang et al. 2011) and Actinopolyspora algeriensis (Meklat et al. Characterization of strain H32T was determined after 2012). The two species ‘A. egyptensis’ (Hozzein and growth at 30 °C for 3 weeks using the media of the Goodfellow 2011) and ‘A. algeriensis’ (Meklat et al. International Streptomyces Project, ISP 2 and ISP 4 2012) were described recently but these names have (Shirling and Gottlieb 1966), and also CM agar (Chun not yet been validated. The genus is characterized by a et al. 2000) and nutrient agar (Waksman 1961). The cell wall of type IVA (meso-diaminopimelic acid, colours of the substrate and aerial mycelia and any arabinose and galactose), a type PIII phospholipid soluble pigments produced were determined by com- pattern (phosphatidylcholine), the presence of MK-9 (H ) 4 parison with ISCC-NBS colour charts (Kelly and Judd and MK-10 (H )orMK-9(H) and MK-9 (H )asthe 4 4 2 1976). The morphological characteristics of strain predominant menaquinones, the presence of iso-C 16:0 H32T, including spore-chain morphology, spore and anteiso-C as the major fatty acids and the absence 17:0 size and surface ornamentation, were assessed by of mycolic acids (Gochnauer et al. 1989;Tangetal. light microscopy (B1, Motic) and scanning electron 2011). The G?C contents of the genomic DNA are microscopy (Hitachi S450). Several physiological 64–69 mol%. tests were used to characterize actinomycete strain. During a study on halophilic actinomycetes from Growth at different temperatures (10, 15, 25, 28, 30, Saharan soils in El-Oued province (south Algeria), 32, 35, 40 and 45 °C), various pH values (5.0, 6.0, 7.0 strain H32T was isolated and purified. The present 8.0 and 9.0 using the buffer system described by Xu study was carried out to determine the taxonomic et al. 2005) and NaCl concentrations (0, 7, 10, 15, 20, status of this strain by using a polyphasic approach. 25, 28, 30, 32 and 35 %; w/v) were determined by using Based on phenotypic and genotypic evidence, it is nutrient agar medium, with the cultures incubated for proposed that the strain H32T represents a novel 21 days at 30 °C. Utilization of carbohydrates and species of the genus Actinopolyspora, for which the decarboxylation of organic acids were evaluated using name Actinopolyspora saharensis sp. nov. is proposed. the method of Gordon et al. (1974). Degradation of different other organic compounds was studied as Materials and methods described by Goodfellow (1971). Lysozyme sensitivity and production of nitrate reductase were determined Isolation and maintenance of isolate according to the methods of Gordon and Barnett (1977) and Marchal et al. (1987), respectively. During an investigation of actinomycete diversity in Saharan soils (Meklat et al. 2011), strain H32T was Chemotaxonomy isolated from a soil sample collected from El-Oued province (33°1905900N, 6°5205900E), south Algeria. For the chemotaxonomic analyses, strain H32T was Isolation was carried out by a dilution-plate method grown in complex broth medium containing 15 % (w/v) 123 Author's personal copy Antonie van Leeuwenhoek (2013) 103:771–776 773 NaCl at 30 °C for 10 days on a rotary shaker shaped spores. The spores were determined to have a (250 rpm). Biomass was harvested by centrifugation smooth surface (Fig. 1) and be non-motile. Growth at 3,500 rpm and washed several times with distilled was found to be weak on ISP 4 medium, with the aerial water. Analysis of diaminopimelic acid and whole-cell mycelium poorly developed.
Load more

Recommended publications
  • Genome Sequencing Identified a Novel Lasso Peptide
    EurAsian Journal of BioSciences Eurasia J Biosci 14, 7933-7941 (2020) Genome sequencing identified a novel lasso peptide Sirilak Namwong1*, Masahiro Yuki2, Takuji. Kudo2, Moriya Ohkuma2 Takashi Itoh2 Somboon Tanasupawat3 1Department of Biotechnology, Faculty of Science and Technology, Suan Sunandha Rajabhat University, Bangkok 10300, Thailand. 2Japan Collection of Microorganisms, RIKEN BioResource Center, 3-1-1 Koyadai, Tsukuba, Ibaraki 305-0074, Japan 3Department of Biochemistry and Microbiology, Faculty of Pharmaceutical Sciences, Chulalongkorn University, Bangkok 10330, Thailand *Corresponding author: [email protected] Abstract Background: One of the biggest problems to global health is rising of antibiotic resistance, however, the novel bioactive compounds are less discovered. To overcome this situation, genome sequencing and annotating have been developed in order to predict the biosynthesis pathway of new drugs. Materials and Methods: Strain BKK1 was classified based on 16S rRNA gene sequencing, ANI- Blast (ANIb), ANI-MUMmer (ANIm) and digital DNA-DNA hybridization (dDDH). The antiSMASH was selected for prediction the secondary metabolism gene clusters. Results: Strain BKK1 was proposed as Actinopolyspora saharensis based on 16S rRNA gene sequence similarity (99.07%), ANIb (98.59%,), ANIm (99.04%) and dDDH (90.9%). The gene clusters of biosynthetic pathways predicted to synthesize a class IV lasso peptide. Its leader region, core sequences and N-terminal end were divergent form class IV lasso peptides. According to the results, an active peptide from A. saharensis BKK1 was assumed to be a new biosynthesis cluster of class IV of lasso peptide. Additionally, its supernatant exhibited inhibitory activity against Escherichia coli ATCC 25922 and Staphylococcus aureus ATCC 25923.
    [Show full text]
  • Actinopolyspora Egyptensis Sp. Nov., a New Halophilic Actinomycete
    African Journal of Microbiology Research Vol. 5(2) pp. 100-105, 18 January, 2011 Available online http://www.academicjournals.org/ajmr DOI: 10.5897/AJMR10.667 ISSN 1996-0808 ©2011 Academic Journals Full Length Research Paper Actinopolyspora egyptensis sp. nov., a new halophilic actinomycete Wael N. Hozzein1,2* and Michael Goodfellow3 1Chair of Advanced Proteomics and Cytomics Research, College of Science, King Saud University, Riyadh, Saudi Arabia. 2Department of Botany, Faculty of Science, Beni-Suef University, Beni-Suef, Egypt. 3Division of Biology, University of Newcastle, Newcastle upon Tyne, NE1 7RU, UK. Accepted 22 December, 2010 A halophilic actinomycete, designated HT371T, was isolated from a soil sample collected from the shore of the salty Lake Qaroun, Egypt, and was the subject of a polyphasic study. Analysis of 16S rRNA indicated that the isolate belonged to the genus Actinopolyspora and constituted a separate clade in the Actinopolyspora 16S rRNA gene tree with similarity values of 96.5 and 96.2% with Actinopolyspora halophila DSM43834T and Actinopolyspora mortivallis DSM44261T, respectively. Isolate HT371T had chemotaxonomic and morphological properties consistent with its classification in the genus Actinopolyspora and could grow on agar plates at NaCl concentrations of up to 25% (w/v). The isolate was readily differentiated from the type strains of genus Actinopolyspora using a range of phenotypic characters. On the basis of polyphasic evidence, the strain HT371T represents a novel species for which the name Actinopolyspora egyptensis sp. nov. is proposed. The type strain is HT371T (=CGMCC 4.2041T). Key words: Actinopolyspora egyptensis sp. nov., halophilic isolate, polyphasic taxonomy. INTRODUCTION The genus Actinopolyspora was created by Gochnauer et taxonomic status, which showed that it merited al.
    [Show full text]
  • Isolation and Diversity of Sediment Bacteria in The
    bioRxiv preprint doi: https://doi.org/10.1101/638304; this version posted May 14, 2019. The copyright holder for this preprint (which was not certified by peer review) is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under aCC-BY 4.0 International license. 1 Isolation and Diversity of Sediment Bacteria in the 2 Hypersaline Aiding Lake, China 3 4 Tong-Wei Guan, Yi-Jin Lin, Meng-Ying Ou, Ke-Bao Chen 5 6 7 Institute of Microbiology, Xihua University, Chengdu 610039, P. R. China. 8 9 Author for correspondence: 10 Tong-Wei Guan 11 Tel/Fax: +86 028 87720552 12 E-mail: [email protected] 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 bioRxiv preprint doi: https://doi.org/10.1101/638304; this version posted May 14, 2019. The copyright holder for this preprint (which was not certified by peer review) is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under aCC-BY 4.0 International license. 29 Abstract A total of 343 bacteria from sediment samples of Aiding Lake, China, were isolated using 30 nine different media with 5% or 15% (w/v) NaCl. The number of species and genera of bacteria recovered 31 from the different media significantly varied, indicating the need to optimize the isolation conditions. 32 The results showed an unexpected level of bacterial diversity, with four phyla (Firmicutes, 33 Actinobacteria, Proteobacteria, and Rhodothermaeota), fourteen orders (Actinopolysporales, 34 Alteromonadales, Bacillales, Balneolales, Chromatiales, Glycomycetales, Jiangellales, Micrococcales, 35 Micromonosporales, Oceanospirillales, Pseudonocardiales, Rhizobiales, Streptomycetales, and 36 Streptosporangiales), including 17 families, 41 genera, and 71 species.
    [Show full text]
  • Of the 16 Strains Obtained from Salty Soil in Mongolia, Six Strains Were
    African Journal of Microbiology Research Vol. 7(4), pp. 298-308, 22 January, 2013 Available online at http://www.academicjournals.org/AJMR DOI: 10.5897/AJMR12.498 ISSN 1996 0808 ©2013 Academic Journals Full Length Research Paper Isolation, classification, phylogenetic analysis and scanning electron microscopy of halophilic, halotolerant and alkaliphilic actinomycetes isolated from hypersaline soil Ismet Ara1,2*, D. Daram4, T. Baljinova4, H. Yamamura5, W. N. Hozzein3, M. A. Bakir1, M. Suto2 and K. Ando2 1Department of Botany and Microbiology, College of Science, King Saud University, P. O. Box 22452, Riyadh-11495, Kingdom of Saudi Arabia. 2Biotechnology Development Center, Department of Biotechnology (DOB), National Institute of Technology and Evaluation (NITE), Kazusakamatari 2-5-8, Kisarazu, Chiba, 292-0818, Japan. 3Bioproducts Research Chair (BRC), College of Science, King Saud University. 4Division of Applied Biological Sciences, Interdisciplinary Graduate School of Medicine and Engineering, University of Yamanashi, Takeda-4, Kofu 400-8511, Japan. Accepted 9 January, 2013 Actinomycetes were isolated by plating of serially diluted samples onto humic acid-vitamin agar prepared with or without NaCl and characterized by physiological and phylogenetical studies. A total of 16 strains were isolated from hypersaline soil in Mongolia. All strains showed alkaliphilic characteristics and were able to abundantly grow in media with pH 9.0. Six strains were halotolerant actinomycetes (0 to 12.0% NaCl) and 10 strains were moderately halophiles (3.0 to 12.0% NaCl). Most strains required moderately high salt (5.0 to 10.0%) for optimal growth but were able to grow at lower NaCl concentrations. Among the 16 strains, 5 were able to grow at 45°C.
    [Show full text]
  • Actinopolyspora Saharensis Sp. Nov., a Novel Halophilic Actinomycete Isolated from a Saharan Soil of Algeria
    View metadata, citation and similar papers at core.ac.uk brought to you by CORE provided by Open Archive Toulouse Archive Ouverte Open Archive Toulouse Archive Ouverte ( OATAO ) OATAO is an open access repository that collects the work of Toulouse researchers and makes it freely available over the web where possible. This is an author-deposited version published in: http://oatao.univ-toulouse.fr/ Eprints ID: 9939 To link to this article : DOI:10.1007/s10482-012-9859-z http://dx.doi.org/10.1007/s10482-012-9859-z To cite this version : Meklat, Atika and Bouras, Noureddine and Zitouni, Abdelghani and Mathieu, Florence and Lebrihi, Ahmed and Schumann, Peter and Spröer, Cathrin and Klenk, Hans-Peter and Sabaou, Nasserdine Actinopolyspora saharensis sp. nov., a novel halophilic actinomycete isolated from a Saharan soil of Algeria. (2013) Antonie van Leeuwenhoek, vol. 103 (n° 4). pp. 771-776. ISSN 0003-6072 Any correspondence concerning this service should be sent to the repository administrator: [email protected] Actinopolyspora saharensis sp. nov., a novel halophilic actinomycete isolated from a Saharan soil of Algeria Atika Meklat • Noureddine Bouras • Abdelghani Zitouni • Florence Mathieu • Ahmed Lebrihi • Peter Schumann • Cathrin Spro¨er • Hans-Peter Klenk • Nasserdine Sabaou Abstract A novel halophilic actinomycete, strain 6.0–7.0 and in the presence of 15–25 % (w/v) NaCl. The H32T, was isolated from a Saharan soil sample collected strain was observed to produce abundant aerial myce- in El-Oued province, south Algeria. The isolate was lium, which formed long chains of rod-shaped spores at characterized by means of polyphasic taxonomy.
    [Show full text]
  • Isolation and Diversity of Sediment Bacteria in The
    bioRxiv preprint doi: https://doi.org/10.1101/638304; this version posted May 14, 2019. The copyright holder for this preprint (which was not certified by peer review) is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under aCC-BY 4.0 International license. 1 Isolation and Diversity of Sediment Bacteria in the 2 Hypersaline Aiding Lake, China 3 4 Tong-Wei Guan, Yi-Jin Lin, Meng-Ying Ou, Ke-Bao Chen 5 6 7 Institute of Microbiology, Xihua University, Chengdu 610039, P. R. China. 8 9 Author for correspondence: 10 Tong-Wei Guan 11 Tel/Fax: +86 028 87720552 12 E-mail: [email protected] 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 bioRxiv preprint doi: https://doi.org/10.1101/638304; this version posted May 14, 2019. The copyright holder for this preprint (which was not certified by peer review) is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under aCC-BY 4.0 International license. 29 Abstract A total of 343 bacteria from sediment samples of Aiding Lake, China, were isolated using 30 nine different media with 5% or 15% (w/v) NaCl. The number of species and genera of bacteria recovered 31 from the different media significantly varied, indicating the need to optimize the isolation conditions. 32 The results showed an unexpected level of bacterial diversity, with four phyla (Firmicutes, 33 Actinobacteria, Proteobacteria, and Rhodothermaeota), fourteen orders (Actinopolysporales, 34 Alteromonadales, Bacillales, Balneolales, Chromatiales, Glycomycetales, Jiangellales, Micrococcales, 35 Micromonosporales, Oceanospirillales, Pseudonocardiales, Rhizobiales, Streptomycetales, and 36 Streptosporangiales), including 17 families, 41 genera, and 71 species.
    [Show full text]
  • A Novel Strain of Actinopolyspora Mortivallis with Antibacterial Activity Isolated from a Saharan Soil
    Ann Microbiol (2012) 62:1049–1057 DOI 10.1007/s13213-011-0346-y ORIGINAL ARTICLE A novel strain of Actinopolyspora mortivallis with antibacterial activity isolated from a Saharan soil Atika Meklat & Nasserdine Sabaou & Noureddine Bouras & Abdelghani Zitouni & Cathrin Spröer & Hans-Peter Klenk & Florence Mathieu & Ahmed Lebrihi Received: 21 March 2011 /Accepted: 29 August 2011 /Published online: 17 September 2011 # Springer-Verlag and the University of Milan 2011 Abstract A new halophilic actinomycete strain, designated hybridization confirmed that it belonged to A. mortivallis. H16, has been isolated from a hypersaline Saharan soil in This strain showed high activity against Klebsiella Ouargla province (southern Algeria) and characterized pneumoniae and optimally secreted antibiotics during the taxonomically using a polyphasic approach. The strain mid-stationary growth phase in liquid culture. The anti- grew at 18–50°C, pH 5–9, and 7–32% (w/v) NaCl. It biotics were extracted with n-butanol and separated on produced abundant aerial mycelia, which formed long silica gel plates by ethanol–ammonia–water (mobile chains of rod-shaped spores at maturity, and fragmented phase). The results of bioautography revealed the presence substrate mycelia. The strain contained chemotaxonomic of five antibiotics. The chemical revelations showed that markers that were diagnostic for the genus Actinopolyspora, these antibiotics were glycosylated polycyclic aromatic such as meso-diaminopimelic acid, arabinose, and galactose, compounds containing amine groups and hydroxamic phosphatidylcholine as a diagnostic phospholipid, and acids. The UV-visible and mass spectra of the most active predominant menaquinones MK-9(H4) and MK-10(H4). antibiotics were determined. The predominant fatty acids were anteiso- and iso-C17:0, iso-C15:0, and 9-methyl-C16:0.
    [Show full text]
  • 1 a Cytoplasmic Peptidoglycan Amidase Homologue Controls Mycobacterial Cell Wall
    1 A cytoplasmic peptidoglycan amidase homologue controls mycobacterial cell wall 2 synthesis 3 4 Cara C. Boutte1, Christina E. Baer2, Kadamba Papavinasasundaram2, Weiru 5 Liu1, Michael R Chase1, Xavier Meniche2, Sarah M. Fortune1, Christopher M. 6 Sassetti2, Thomas R. Ioerger3, Eric J. Rubin1,4,* 7 8 1Department of Immunology and Infectious Disease, Harvard T.H. Chan School 9 of Public Health, Boston, MA 02115, USA 10 2Department of Microbiology and Physiological Systems, University of 11 Massachusetts Medical School, Worcester, MA 01655, USA 12 3Department of Computer Science, Texas A&M University, College Station, TX 13 77842, USA 14 4Department of Microbiology and Immunobiology, Harvard Medical School, 15 Boston, MA 02115, USA 16 *correspondence: [email protected] 17 18 Author contributions: Conceptualization, C.C.B. and E.J.R.; Methodology, C.C.B., 19 C.E.B., M.R.C. and E.J.R.; Investigation, C.C.B., T.R.I., W.L., X.M., K.P.; 20 Resources, C.C.B., C.E.B., K.P., C.M.S., E.J.R.; Writing-Original Draft, C.C.B.; 21 Writing-Review & Editing, C.C.B., T.R.I., C.M.S., C.E.B., E.J.R.; Visualization, 22 C.C.B.; Supervision, S.R.M., C.M.S., E.J.R.; Funding acquisition, E.J.R., C.C.B. 23 1 24 Abbreviations: Mtb = Mycobacterium tuberculosis. Msmeg = Mycobacterium 25 smegmatis. PG = peptidoglycan. Protein and gene names refer to the M. 26 smegmatis homologs. When the Mtb proteins and genes are discussed, they are 27 indicated, e.g.: CwlMTB.
    [Show full text]
  • Genome-Based Taxonomic Classification of the Phylum
    ORIGINAL RESEARCH published: 22 August 2018 doi: 10.3389/fmicb.2018.02007 Genome-Based Taxonomic Classification of the Phylum Actinobacteria Imen Nouioui 1†, Lorena Carro 1†, Marina García-López 2†, Jan P. Meier-Kolthoff 2, Tanja Woyke 3, Nikos C. Kyrpides 3, Rüdiger Pukall 2, Hans-Peter Klenk 1, Michael Goodfellow 1 and Markus Göker 2* 1 School of Natural and Environmental Sciences, Newcastle University, Newcastle upon Tyne, United Kingdom, 2 Department Edited by: of Microorganisms, Leibniz Institute DSMZ – German Collection of Microorganisms and Cell Cultures, Braunschweig, Martin G. Klotz, Germany, 3 Department of Energy, Joint Genome Institute, Walnut Creek, CA, United States Washington State University Tri-Cities, United States The application of phylogenetic taxonomic procedures led to improvements in the Reviewed by: Nicola Segata, classification of bacteria assigned to the phylum Actinobacteria but even so there remains University of Trento, Italy a need to further clarify relationships within a taxon that encompasses organisms of Antonio Ventosa, agricultural, biotechnological, clinical, and ecological importance. Classification of the Universidad de Sevilla, Spain David Moreira, morphologically diverse bacteria belonging to this large phylum based on a limited Centre National de la Recherche number of features has proved to be difficult, not least when taxonomic decisions Scientifique (CNRS), France rested heavily on interpretation of poorly resolved 16S rRNA gene trees. Here, draft *Correspondence: Markus Göker genome sequences
    [Show full text]
  • Actinobacteria Derived from Algerian Ecosystems As a Prominent Source of Antimicrobial Molecules
    antibiotics Review Actinobacteria Derived from Algerian Ecosystems as a Prominent Source of Antimicrobial Molecules Ibtissem Djinni 1,2,*, Andrea Defant 2, Mouloud Kecha 1 and Ines Mancini 2,* 1 Laboratoire de Microbiologie Appliquée, Faculté des Sciences de la Nature et de la Vie, Université de Bejaia, Targa Ouzemmour 06000, Algeria; [email protected] 2 Bioorganic Chemistry Laboratory, Department of Physics, University of Trento, 38123 Trento, Italy; [email protected] * Correspondence: [email protected] (I.D.); [email protected] (I.M.); Tel.: +39-0461-281548 (I.D.) Received: 6 September 2019; Accepted: 25 September 2019; Published: 1 October 2019 Abstract: Actinobacteria, in particular “rare actinobacteria” isolated from extreme ecosystems, remain the most inexhaustible source of novel antimicrobials, offering a chance to discover new bioactive metabolites. This is the first overview on actinobacteria isolated in Algeria since 2002 to date with the aim to present their potential in producing bioactive secondary metabolites. Twenty-nine new species and one novel genus have been isolated, mainly from the Saharan soil and palm groves, where 37.93% of the most abundant genera belong to Saccharothrix and Actinopolyspora. Several of these strains were found to produce antibiotics and antifungal metabolites, including 17 new molecules among the 50 structures reported, and some of these antibacterial metabolites have shown interesting antitumor activities. A series of approaches used to enhance the production of bioactive compounds is also presented as the manipulation of culture media by both classical methods and modeling designs through statistical strategies and the associations with diverse organisms and strains. Focusing on the Algerian natural sources of antimicrobial metabolites, this work is a representative example of the potential of a closely combined study on biology and chemistry of natural products.
    [Show full text]
  • Actinopolyspora Righensis Sp. Nov., a Novel Halophilic Actinomycete
    Actinopolyspora righensis sp. nov., a novel halophilic actinomycete isolated from Saharan soil in Algeria Atika Meklat, Noureddine Bouras, Abdelghani Zitouni, Florence Mathieu, Ahmed Lebrihi, Peter Schumann, Cathrin Spröer, Hans-Peter Klenk, Nasserdine Sabaou To cite this version: Atika Meklat, Noureddine Bouras, Abdelghani Zitouni, Florence Mathieu, Ahmed Lebrihi, et al.. Actinopolyspora righensis sp. nov., a novel halophilic actinomycete isolated from Saharan soil in Al- geria. Antonie van Leeuwenhoek, Springer Verlag, 2013, vol. 104 (n° 3), pp. 301-307. 10.1007/s10482- 013-9948-7. hal-01177618 HAL Id: hal-01177618 https://hal.archives-ouvertes.fr/hal-01177618 Submitted on 17 Jul 2015 HAL is a multi-disciplinary open access L’archive ouverte pluridisciplinaire HAL, est archive for the deposit and dissemination of sci- destinée au dépôt et à la diffusion de documents entific research documents, whether they are pub- scientifiques de niveau recherche, publiés ou non, lished or not. The documents may come from émanant des établissements d’enseignement et de teaching and research institutions in France or recherche français ou étrangers, des laboratoires abroad, or from public or private research centers. publics ou privés. Open Archive Toulouse Archive Ouverte (OATAO) OATAO is an open access repository that collects the work of Toulouse researchers and makes it freely available over the web where possible. This is an author-deposited version published in: http://oatao.univ-toulouse.fr/ Eprints ID: 9759 DOI:10.1007/s10482-013-9948-7 Official URL: http://dx.doi.org/10.1007/s10482-013-9948-7 To cite this version: Meklat, Atika and Bouras, Noureddine and Zitouni, Abdelghani and Mathieu, Florence and Lebrihi, Ahmed and Schumann, Peter and Spröer, Cathrin and Klenk, Hans-Peter and Sabaou, Nasserdine Actinopolyspora righensis sp.
    [Show full text]
  • Actinopolyspora Algeriensis Sp. Nov., a Novel Halophilic Actinomycete Isolated from a Saharan Soil
    Actinopolyspora algeriensis sp. nov., a novel halophilic actinomycete isolated from a Saharan soil Atika Meklat, Noureddine Bouras, Abdelghani Zitouni, Florence Mathieu, Ahmed Lebrihi, Peter Schumann, Cathrin Spröer, Hans-Peter Klenk, Nasserdine Sabaou To cite this version: Atika Meklat, Noureddine Bouras, Abdelghani Zitouni, Florence Mathieu, Ahmed Lebrihi, et al.. Actinopolyspora algeriensis sp. nov., a novel halophilic actinomycete isolated from a Saharan soil. Extremophiles, Springer Verlag, 2012, vol. 16, pp. 771-776. 10.1007/s00792-012-0473-9. hal- 00927627 HAL Id: hal-00927627 https://hal.archives-ouvertes.fr/hal-00927627 Submitted on 13 Jan 2014 HAL is a multi-disciplinary open access L’archive ouverte pluridisciplinaire HAL, est archive for the deposit and dissemination of sci- destinée au dépôt et à la diffusion de documents entific research documents, whether they are pub- scientifiques de niveau recherche, publiés ou non, lished or not. The documents may come from émanant des établissements d’enseignement et de teaching and research institutions in France or recherche français ou étrangers, des laboratoires abroad, or from public or private research centers. publics ou privés. Open Archive Toulouse Archive Ouverte (OATAO) OATAO is an open access repository that collects the work of Toulouse researchers and makes it freely available over the web where possible. This is an author-deposited version published in: http://oatao.univ-toulouse.fr/ Eprints ID: 9996 To link to this article : DOI:10.1007/s00792-012-0473-9 URL: http://dx.doi.org/10.1007/s00792-012-0473-9 To cite this version: Meklat, Atika and Bouras, Noureddine and Zitouni, Abdelghani and Mathieu, Florence and Lebrihi, Ahmed and Schumann, Peter and Spröer, Cathrin and Klenk, Hans-Peter and Sabaou, Nasserdine Actinopolyspora algeriensis sp.
    [Show full text]