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ARG41441 Package: 100 μl anti-Ret antibody Store at: -20°C

Summary

Product Description Rabbit Polyclonal antibody recognizes Ret

Tested Reactivity Hu, Ms, Rat

Tested Application ICC/IF, IHC-P, IP, WB

Host Rabbit

Clonality Polyclonal

Isotype IgG

Target Name Ret

Antigen Species Human

Immunogen Synthetic peptide of Human Ret.

Conjugation Un-conjugated

Alternate Names RET51; CDHF12; HSCR1; Proto- c-Ret; PTC; Proto-oncogene tyrosine- receptor Ret; RET-ELE1; CDHR16; MEN2B; MEN2A; MTC1; EC 2.7.10.1; Cadherin family member 12

Application Instructions

Application table Application Dilution

ICC/IF 1:50 - 1:200

IHC-P 1:50 - 1:200

IP 1:50

WB 1:500 - 1:2000

Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Positive Control SH-SY5Y

Calculated Mw 124 kDa

Properties

Form Liquid

Purification Affinity purified.

Buffer PBS (pH 7.4), 150 mM NaCl, 0.02% Sodium azide and 50% Glycerol.

Preservative 0.02% Sodium azide

Stabilizer 50% Glycerol

Storage instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw www.arigobio.com 1/2 cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.

Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Gene Symbol RET

Gene Full Name ret proto-oncogene

Background This gene, a member of the cadherin superfamily, encodes one of the receptor tyrosine , which are cell-surface molecules that transduce signals for cell growth and differentiation. This gene plays a crucial role in neural crest development, and it can undergo oncogenic activation in vivo and in vitro by cytogenetic rearrangement. Mutations in this gene are associated with the disorders multiple endocrine neoplasia, type IIA, multiple endocrine neoplasia, type IIB, Hirschsprung disease, and medullary thyroid carcinoma. Two transcript variants encoding different isoforms have been found for this gene. Additional transcript variants have been described but their biological validity has not been confirmed. [provided by RefSeq, Jul 2008]

Function Receptor tyrosine- involved in numerous cellular mechanisms including cell proliferation, neuronal navigation, cell migration, and cell differentiation upon binding with glial cell derived neurotrophic factor family ligands. Phosphorylates PTK2/FAK1. Regulates both cell death/survival balance and positional information. Required for the molecular mechanisms orchestration during intestine organogenesis; involved in the development of enteric nervous system and renal organogenesis during embryonic life, and promotes the formation of Peyer's patch-like structures, a major component of the gut-associated lymphoid tissue. Modulates cell adhesion via its cleavage by caspase in sympathetic neurons and mediates cell migration in an (e.g. ITGB1 and ITGB3)-dependent manner. Involved in the development of the neural crest. Active in the absence of ligand, triggering apoptosis through a mechanism that requires receptor intracellular caspase cleavage. Acts as a dependence receptor; in the presence of the ligand GDNF in somatotrophs (within pituitary), promotes survival and down regulates growth hormone (GH) production, but triggers apoptosis in absence of GDNF. Regulates nociceptor survival and size. Triggers the differentiation of rapidly adapting (RA) mechanoreceptors. Mediator of several diseases such as neuroendocrine cancers; these diseases are characterized by aberrant -regulated cell migration. [UniProt]

Cellular Localization Cell membrane; Single-pass type I membrane protein. Endosome membrane; Single-pass type I membrane protein. [UniProt]

Images

ARG41441 anti-Ret antibody WB image

Western blot: SH-SY5Y cell lysate stained with ARG41441 anti-Ret antibody.

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