A survey on health and mortality causative agent of Pinctada margaritifera produced in Bander Lengh research station for mollusc

Item Type monograph

Authors Afsharnasab, Mohammad; Esmailzadeh, A.; Argangi, Gh.; Mehrabi, M.R.; Radkhah, K.; Zemani, M.; Kakoolaki, S.; Shrifpour, E.; Haribghalehtaki, Z.; Nikbakhtmobarakeh, M.; Ghaednia, B.; Ahteshamei, F.; Amiri, J.; Kamali, E.; Seidmoradi, Sh.; Karimzadeh, R.; Gozari, M.; Malakoti, M.; Rajabi, I.; Mohommadian, A.

Publisher Iranian Fisheries Science Research Institute

Download date 02/10/2021 09:59:14

Link to Item http://hdl.handle.net/1834/39765

: ﺑﺮرﺳﻲ وﺿﻌﻴﺖ ﺑﻬﺪاﺷﺘﻲ و ﻋﻠﻞ ﺗﻠﻔﺎت ﺻﺪﻓﻬﺎي ﻣﺮوارﻳﺪ ﺳﺎز ﻟﺐ ﺳﻴﺎه (Pinctada margaritifera ) ﺗﻮﻟﻴﺪي در اﻳﺴﺘﮕﺎه ﺗ ﺤﻘﻴﻘﺎت ﻧﺮم ﺗﻨﺎن ﺑﻨﺪرﻟﻨﮕﻪ

:

42039

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(Pinctada margaritifera )

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. 19 91 17/6/

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.

. .

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.

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.

. shell boring mussle clionid sponges

.

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2000 1998 Hine and Thorne . 1987)

(Haplosporida)

. Multinucleated Spherix X

Perkinsis marinus . (Vblayudhan,1983)

.

Gregarine, intracellular .

.(Melba etal ,2007) ciliate, Haplosporidum, perkinus, Apicomplex, Cryptosporidia, Cestods and Metazoa

Parvolike virus 1998 Humphery

2000 Kitamura 1998 Suzuki Kamakura and Kusuda .

. Birnavirus

OIE /// 444/ 4

.

Intranuclear Papova like virus Birnavirus parvolike virus

. (Melba etal ,2007; OIE,2008). viral inclusion

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.

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.

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. etal ,2007; OIE,2008).

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Vibrio tapetis

seawater cytophaga agar Cytophaga .

Aeromonas ,Psudomonas . TCBS MA Vibrio

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Catalase + + + +

Indol + + + +

Methyl red ̶ + ̶

Voges-proskauer ̶ + ̶ +

O/F + + + +

Citrate ̶ + ̶ +

NO2 + + + +

Gelatinase + + + +

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1: Test Bacterial isolates V.harveyi V.alginolyticus V.splendidus I V.anguillarum Swarming ̶ + ̶ ̶

Growth at 0% NaCl ̶ ̶ ̶ ̶

Growth at 3% NaCl + + + +

Growth at 6% NaCl + + + +

Growth at 8% NaCl + + ̶ ̶

Growth at 4° C ̶ ̶ ̶ ̶

Growth at 20° C + + + +

Growth at 30° C + + + +

Growth at 35° C + + ̶ +

Acid from glucose + + + +

Acid from sucrose + + ̶ +

Acid from arabinose ̶ ̶ ̶ +

Acid from lactose ̶ ̶ ̶ ̶

Acid from manitol + + + + /// 121212/ 12

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Arginine dihydrolase ̶ ̶ + +

Lysine decarboxylase + + ̶ ̶

Ornithine + + ̶ decarboxylase

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7.

33

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30

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/// 141414/ 14

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Eunicidae, ( 8 ) Proceraea Syllidae 89:

9(. ) Paramorphysia 151515 15 /...

( 10 ) Neanthes Nereidadae : 11 10

( 11 )Nereididae,

43

.

. 25 /// 161616/ 16

1 2:

1

(+) () (+) 4 (+) 4 (+) TCBS 1

(+) (+) (+) (+) (+) TSA 2

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2

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(+) (+) (+) (+) (+) TSA 2

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3 3:

3 3 3

(+) (+) (+) (+) (+) (+) TCBS 1

(+) (+) (+) (+) (+) (+) TSA 2

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. 6

. 7

. 8

T1 umbo 9

10

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T2 D 13

14 ٱ

/// 181818/ 18

5:

efu/ml efu/ml) Aspergllus sp. 001 90001 Aspergllus rilger MBL Cladosporlum sp.

Cladosporlum 002 90002 sp Alternarla sp MBL Peniclliumsp Yeast colony 191919 19 /...

( slldeculture Subculture )

efu/ml)

efu/ml Aureobasldiu T1 90001 MBL m pullulans

Aspergillus T2 90002 MBL sp

Rhodotorula T3 90003 MBL rubra Cladasporiu m sp. Alternaria sp.

Aspergillus 90004 MBL niger

Aspergillus 90005 MBL nlger Aspergillus 3 flowus Mucor sp.

53

.

15 14 13 12

( 16 /// 202020/ 20

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H&E,400µm 14:

. H&E,400µm 15:

. H&E,400µm 16: 212121 21 /...

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.

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.

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.

.

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.

.

.

. (Fragility)

.

.

.

.

. (Blister)

.

.

.

.

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P.radiata Pinctada margaritifera, P.fucata

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Clinoid sponges (Cliona vastitca , C.carpenteri )

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.

.

.

Vibrio tapetis(Vibrio P1)

P. margartifera Manihiki

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Perkins, 1996 .

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.

.

.

Ostracoblable implexa (Mao che etal, 1994)

. P.margaritifera

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Jones(2007)

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.

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/// 262626/ 26

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• dentification of environmental Vibrio species. J of App. Bact. 1994; 76Alsina, M, Blanch, A. R., . A set of keys for biochemical i, 79-85 • Alagarswami, K.& Chellani, A. 1976. On fouling and bouring organisms and motality of pearl oyster in the farm at Veppalodai, Gulf of Mannar. Indian J. Fish.23:10-22. • Baumann, P. and R. H. W. Schubert. (1984) Vibrionaceae Veron. in N. R. Krieg and J. G. Holt, (eds). Bergey’s manual of systematic bacteriology. Williams and Wilkins, Baltimore, Maryland, USA, pp 516–550 • Dourdi,M.s. 1993. Infection of pearl oyster Pinctata margaritifera with Cliona SP. and subsequent destruction of oyster shell. Iranian Fishery bulletin 3;4. • Dourdi,M.s. 1994. Infection of pearl oyster by the boring and fouling organism in the pearl culture farm and natural beds from northern coast of Persian Gulf. Journal of shellfish Research. 13:333 • Dourdi,M.s. 1996. Infestation of the pearl oyster by the boring and fouling organism in the pearl culture farm and natural beds from northern coast of Persian Gulf.In dian Journal of Marine Science 25: 169- 169. • Hine, P.M. & Thorne,T. 1998. Haplosporidium sp. (Haplosporidia) in hatchery and reared pearl oyster Pinctada maxima in Western Australia. Journal of Invertebrate Pathology 71: 48-52. • Hine, P.M. & Thorne,T. 2000. A survey of some parasite and disease of several species of bivalve mollusc in northern Western Australia. Disease of Aquatic Organism 40:67-78. • Humphery, J.D., Norton, J.H., Johns,J.B., Barton,M.A., Connell,M.T., Shelley, C.C & Creeper,J.H .1998. Pearal oyster (Pinctada maxima) aquaculture. Health survey of northern territory, Western Austerlia and Quensland peral oyster beds and Farm. Fisheries Research Development country report. Final report 94/079. • Jones,J.B. 2007. Review of pearl oyster mortalities and disease problem. FAO. Fisheries Technical Paper. No 503, Rome,FAO,2007. • Kitamura,S.L., Jung,S.L & Suzuki,S. 2000. Seasonal change of infective state of marine birnavirus in Japanese pearl oyster Pinctada fucata . Archive of Virology 145. 2003-2014. • Melba, G. B., Sharon, E. M and Franck, C.J. 2007. Pearl oyster health management. A manual. Food and Agriculture Organization of the United Nation. Room, 2007. • OIE,2008. Manual for diagnostic and laboratory standard. OIE publication. 2008. • Paillard, C., Maes,p&Oubella,R 1994. Brown ring disease in clams, Annual Review of Fish Disease 4:219-240. • Pass, D.A., Dybdal,R & Mannion ,M.M.1987. Investigation into the causes of mortality of pearl oyster Pinctada maxima in Western Austerlia. Aquacukture 65: 166-167. • Perkins, F.O. 1996. Shell disease in the gold lip pearl oyster , Pinctata maxima and the European oyster, Grassoctera virginica. Aquatic Living Resources 9: 155-163. • Suzuki, S., Kamakura, M & Kusda,R.1998. Isolation of birnavirus from Japanese pearl oyster Pinctada fucata . Fisheries Science 64: 342-343. • Vblayudhan,T.S 1983. On the occurrence of shell boring polycheat and sponges on pearl oyster Pinctada fucata and control of boring organism. Prceding of the Symposium on coastal Aquaculture 2: 614-618.

/// 282828/ 28

Abstract:

The present study was carried out to investigate the status of health and disease problem in pearl oyster hatcheries in Bander Lengh in Hormozgan Province. From July to September of 2010, 200 samples were collected from different stages (eggs, Dshape, larva, Umbo stage eyespot and Adult) of P. margartifera in Bander lengh in Hormozgan Province. The samples divided to three groups. One group was used for studied the gross and internal observation. The results showed different malformation such as fouling organism, worms, adhesion, color changes and etc. The second group was used for probable bacteria and fungi infestation by the selected media such as TSA, MD (bacteria) and (S.D.A and P.Y.G) for fungi. The main identified bacteria and fungi were consist of Vibrio harveyi , V. alginolytics and V. splendidus and for Fungi Saprolgnia, Alternaria and Chlydosporai, respectively. In this review we also found different polycot in the surface of oysters. The organs examined for histopathology showed no major problem. Key words : Health, Disease, P. margartifera , Bander Lengh.

Ministry of Jihad – e – Agriculture AGRICULTURAL RESEARCH, EDUCATION & EXTENSION ORGANIZATION IRANIAN FISHERIES RESEARCH ORGANIZATION – Persian Gulf and Oman Sea Ecology Research Center

Title : A Survey on Health and Mortality Causative Agent of Pinctada margaritifera Produced in Bander Lengh Research Station for Mollusc Approved Number: 12-75-12-8912-89150 Author: Mohammad Afsharnasab Executor : Mohammad Afsharnasab Collaborator : A.Esmailzadeh,Gh. Argangi, M.R.Mehrabi,K.Radkhah,M. Zemani, Sh. Kakolaki,E.Shrifpour, Z. haribghalehtaki,M.Nikbakhtmobarakeh, B.Ghaednia, F.Ahteshamei,J.Amiri,E.Kamali,Sh.Seidmoradi,R.Karimzadeh,M.Gozari,M.Malakoti,I.Rajabi ,A.Mohommadian Advisor(s): M.Savehdorodi Supervisor: A.Sepahdari Location of execution : Hormozgan province Date of Beginning : 2011 Period of execution : 1 Year & 3 Months Publisher : Iranian Fisheries Research Organization Date of publishing : 2013

All Right Reserved . No Part of this Publication May be Reproduced or Transmitted without indicating the Original Reference

MINISTRY OF JIHAD - E - AGRICULTURE AGRICULTURAL RESEARCH, EDUCATION & EXTENSION ORGANIZATION IRANIAN FISHERIES RESEARCH ORGANIZATION

Title: A Survey on Health and Mortality Causative Agent of Pinctada margaritifera Produced in Bander Lengh Research Station for Mollusc

Executor : Mohammad Afsharnasab

Registration Number

42039