Randomized Complete Block Designs
Block Designs Design Control Completely Random Blocked I II III IV I II III IV Trt 2 Trt 1 Trt 4 Trt 1 Trt 2 Trt 1 Trt 4 Trt 1 Trt 3 Trt 3 Trt 1 Trt 4 Trt 1 Trt 3 Trt 3 Trt 4 Trt 3 Trt 2 Trt 1 Trt 4 Trt 3 Trt 4 Trt 1 Trt 2 Trt 4 Trt 3 Trt 2 Trt 2 Trt 4 Trt 2 Trt 2 Trt 3 Yij = + Ti + (i)j Yijk = + Bi + Tj + εij
RCBD ANOVA Comparison with CRD
CRD RCBD Source df SS Source df SS
I I JYY() 2 2 Ti I - 1 i... Bi I - 1 JYY()i... i 1 i 1 IJ J ()YY 2 2 (i)j I(J - 1) ij i. Tj J – 1 IYY()...j ij11 j 1
IJ IJ ()YY 2 ()YYYY 2 Total IJ - 1 ij .. ij (I -1)(J - 1) ij i.. j .. ij11 ij11
IJ 2 Total IJ - 1 ()YYij .. ij11
Agronomy 526 / Spring 2021 1 Randomized Complete Block Designs
RCBD Blocking Criteria
Spatial • Topography • Edaphic • Windbreak • Flood irrigation (moisture gradient) Temporal • Time Period • Order Other • Greenhouse • Growth chamber • Lab run / Batch
RCBD Blocking Patterns
Trt 1 Trt 4 Trt 2 Trt 3
Trt 4 Trt 3 Trt 2 Trt 1
Gradient Trt 3 Trt 1 Trt 4 Trt 2
Trt 2 Trt 3 Trt 4 Trt 1
Agronomy 526 / Spring 2021 2 Randomized Complete Block Designs
RCBD Blocking Patterns Trt 3 Trt 2 Trt 1 Trt 3 Trt 1 Trt 4 Trt 2 Trt 2 Trt 3 Trt 4 Trt 1 Trt 1 Trt 4 Trt 2 Trt 3 Trt 4
Gradient
RCBD Blocking Patterns Trt 1 Trt 4 Trt 2 Trt 3 Trt 2 Trt 3 Trt 4 Trt 1 Trt 3 Trt 2 Trt 1 Trt 4 Trt 3 Trt 1 Trt 4 Trt 2
Agronomy 526 / Spring 2021 3 Randomized Complete Block Designs
RCBD Linear Additive Model
Yij = + Bi + (i) + Tj + BTij
Where: th Yij =Observation from the ij plot =Overall mean th Bi =Effect of the i block (i) = Restriction error associated with B th Tj =Effect of the j treatment th BTij =Interaction effect of the i of block with the jth treatment
RCBD Expected Mean Squares
Blocks Random Source b t EMS R F i j 2 2 2 Bi 1t + t + t B 2 2 (i) 1t + t 2 2 Tj b0 + BT + b(T) 2 2 BTij 10 + BT
Agronomy 526 / Spring 2021 4 Randomized Complete Block Designs
RCBD Example Kenaf Variety Trial
Block Variety1 234Mean Dowling 4.3 3.7 4.0 2.8 3.7 Evergl41 3.4 3.7 3.9 3.3 3.6 Evergl71 3.6 3.0 3.7 3.6 3.5 Gregg 3.7 4.2 4.3 2.8 3.7 PF2 2.6 2.5 3.2 1.7 2.5 SF459 3.2 5.3 2.2 2.2 3.2 T2 5.4 6.1 4.2 4.6 5.1 Whitten 4.5 3.4 3.3 2.6 3.4 Mean 3.8 4.0 3.6 2.9 3.6
RCBD Example Kenaf Variety Trial ANOVA CRD Mean F Source DF SS Square Value Pr > F Variety 7 14.53005 2.075721 3.40 0.0115 Error 24 14.66376 0.61099 RCBD Mean F Source DF SS Square Value Pr > F Block 3 5.161725 1.720575 Var 7 14.53005 2.075721 4.59 0.003 Error 21 9.502032 0.452478
Agronomy 526 / Spring 2021 5 Randomized Complete Block Designs
RCBD Example Kenaf Variety Trial
CRD 2(.611) LSD 2.063 1.14 .05,24df 4 RCBD 2(.452) LSD 2.079 0.99 .05,21df 4
RCBD Relative Efficiency
(1)(3)nnMS RE BC eC (1)(3)nnMSCB eB
Where: MSC = Error mean square for CRD
MSB = Error mean square for RCBD
nC = error df for CRD
nB = error df for RCBD
Agronomy 526 / Spring 2021 6 Randomized Complete Block Designs
RCBD Example Kenaf Variety Trial
(21 1)(24 3)0.611 RE 1.34 (24 1)(21 3)0.453
Where: MSC = 0.611
MSB = 0.453
nC = 24
nB = 21
RCBD ANOVA Analysis –Barley Example Petersen, 1994
Blocks (Soil Types) Treatment Mean 1234
1(NH4)2SO4 32.1 35.6 41.9 35.4 36.25
2NH4NO3 30.1 31.5 37.1 30.8 32.38
3CO(NH2)2 25.4 27.1 33.8 31.1 29.35
4Ca(NO3)2 24.1 33.0 35.6 31.4 31.03
5NaNO3 26.1 31.0 33.8 31.9 30.70 6 Control 23.2 24.8 26.7 26.7 25.35
Agronomy 526 / Spring 2021 7 Randomized Complete Block Designs
RCBD ANOVA Analysis –Barley Example RCBD Source DF SS MS FP>F Block 3 192.748 64.249 21.1 <.0001 Treatment 5 256.153 51.231 16.9 <.0001 Error 15 45.617 3.041 Total 23 494.518 CRD Source DF SS MS FP>F Treatment 5 256.153 51.231 3.9 0.0148 Error 18 238.365 13.243 Total 23 494.518
RCBD ANOVA Analysis –Barley Example
RCBD 2(3.041) LSD 2.132 2.63 .05,15 df 4
CRD 2(13.243) LSD 2.101 5.41 .05,18 df 4
Agronomy 526 / Spring 2021 8 Randomized Complete Block Designs
RCBD ANOVA Analysis –Barley Example
(15 1)(18 3)13.243 Efficiency 4.28 (18 1)(15 3)3.041
Where: MSC = 13.243
MSB = 3.041
nC = 18
nB = 15
RCBD Expected Mean Squares
Blocks Fixed Source b t EMS F F i j 2 2 Bi 0t + t + t(B) 2 2 (i) 1t + t 2 Tj b0 + b(T) 2 BTij 00 + BT)
Agronomy 526 / Spring 2021 9 Randomized Complete Block Designs
Generalized RCBD Field Layout
Trt 1 Trt 1 Trt 2 Trt 3 Trt 2 Trt 3 Trt 4 Trt 4
Trt 4 Trt 3 Trt 2 Trt 1 Trt 1 Trt 4 Trt 2 Trt 3
Gradient Trt 2 Trt 3 Trt 4 Trt 1 Trt 3 Trt 1 Trt 4 Trt 2
Trt 2 Trt 1 Trt 4 Trt 1 Trt 4 Trt 3 Trt 2 Trt 3
Generalized RCBD Model
Yijk = + Bi + (i) + Tj + BTij + (ij)k Where: th Yijk =Observation from the ijk plot =Overall mean th Bi =Effect of the i block. (i) = Restriction error associated with B th Tj =Effect of the j treatment th BTij =Interaction effect of the i of block with the jth treatment. th th (ij)k =Effect of the k eu within i block and jth treatment.
Agronomy 526 / Spring 2021 10 Randomized Complete Block Designs
Generalized RCBD Expected Mean Squares
Blocks Random Source b t r EMS R F R i j k 2 2 2 Bi 1tr + tr + tr B 2 2 (i) 1tr + tr 2 2 Tj b0r + r BT + br(T) 2 2 BTij 10r + r BT 2 (ij)k 111
Generalized RCBD Expected Mean Squares
Blocks Fixed Source b t r EMS F F R i j k 2 2 Bi 0tr + tr + tr(B) 2 2 (i) 1tr + tr 2 Tj b0r + br(T) 2 BTij 00r + rBT) 2 (ij)k 111
Agronomy 526 / Spring 2021 11 Randomized Complete Block Designs
Fields as Blocks pp. 95‐103, Lorenzen and Anderson Layout: Field 12 Fertilizer 12345 12345 16... 2631... Plot 27 . . . 27. . . . 38...... 49..24....49 510. 2025 . . . 4550
Fields as Blocks pp. 95‐103, Lorenzen and Anderson
Yijk = + Fi + (i) + Nj + FNij + (ij)k 2 5 5 R F R Source i j kEMS 2 2 2 Fi 155 + 25 + 25 F 2 2 (i) 155 + 25 2 2 Nj 205 + 5 FN + 10(F) 2 2 FNij 105 + 5 FN 2 (ij)k 111
Agronomy 526 / Spring 2021 12