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Cell Cycle-Regulated Nuclear Localization of M CM2 and M CM3, Which Are Required for the Initiation of DNA Synthesis at Chromosomal Replication Origins in Yeast

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Cell Cycle-Regulated Nuclear Localization of M CM2 and M CM3, Which Are Required for the Initiation of DNA Synthesis at Chromosomal Replication Origins in Yeast Downloaded from genesdev.cshlp.org on September 28, 2021 - Published by Cold Spring Harbor Laboratory Press Cell cycle-regulated nuclear localization of M CM2 and M CM3, which are required for the initiation of DNA synthesis at chromosomal replication origins in yeast Hong Yan, 1 A. Margaret Merchant, and Bik K.Tye Section of Biochemistry, Molecular and Cell Biology, Comell University, Ithaca, New York 14853 USA MCM2 and MCM3 are two genetically interacting and structurally related proteins essential for growth in Saccharomyces cerevisiae. Mutants defective in these proteins affect the stability of minichromosomes in general, but the severity of the defect is dependent on the autonomously replicating sequence (ARS) that drives the replication of that plasmid. In this paper we show by two-dimensional gel electrophoresis that the initiation of DNA synthesis at chromosomal replication origins is also reduced in frequency in these mutants. We show further that the nuclear and subnuclear localizations of the MCM2 and MCM3 proteins are temporally regulated with respect to the cell cycle. These proteins enter the nucleus at the end of mitosis, persist there throughout G~ phase, and disappear from it at the beginning of S phase. Once inside the nucleus, a fraction of the MCM2 and MCM3 proteins becomes tightly associated with DNA. The association of MCM2 and MCM3 with chromatin presumably leads to the initiation of DNA synthesis, and their subsequent disappearance from the nucleus presumably prevents reinitiation of DNA synthesis at replication origins. This temporally and spatially restricted localization of MCM2 and MCM3 in the nucleus may serve to ensure that DNA replication occurs once and only once per cell cycle. [Key Words: MCM2; MCM3; S. cerevisiae; nuclear localization; initiation of DNA synthesis; DNA replication; licensing factors] Received July 13, 1993; revised version accepted August 24, 1993. Regulation of DNA replication in eukaryotes is dove- into the nucleus unless the nuclear envelope is per- tailed with stages of the cell cycle. In each cell cycle, (1) turbed. In this way, cells can effectively block re-repli- replication occurs only during S phase, (2) chromosomal cation of their genome and then erase the block by going re-replication is prevented, (3) mitosis is delayed until through mitosis. The concept of a licensing factor can replication is completed, and (4) the re-replication block easily explain the results of earlier cell fusion studies is removed before the next S phase. The coupling be- that G1 nuclei, but not G2 nuclei, can initiate DNA rep- tween completion of DNA replication and mitosis is rel- lication when fused with S cells (Rao and Johnson 1970). atively well understood. Replicating DNA sends out a However, this hypothesis has two drawbacks. First, the negative signal that suppresses the activation of MPF, molecular nature of such a licensing factor, if it exists, the mitosis (or maturation) promoting factor (Dasso and has yet to be defined. Second, in lower eukaryotes, such Newport 1990; Smythe and Newport 1992). In contrast, as the budding yeast Saccharomyces cerevisiae, the nu- little is known about other mechanisms that coordinate clear membrane does not break down during mitosis. DNA replication with the cell cycle. S. cerevisiae offers an excellent model system for Using Xenopus egg extracts, Blow and Laskey (1988) studying DNA replication and its control in eukaryotes. found that to get a second round of replication, nuclei Replication origins isolated from their native chromo- either have to go through mitosis, during which the nu- somes can function as autonomously replicating se- clear envelope breaks down and reassembles, or be per- quences (ARSs) when cloned in plasmids (Brewer and meabilized with nonionic detergents. This observation Fangman 1987; Huberman et al. 1988). We have identi- suggests the existence of an essential replication factor, fied a number of mutants, called mcm, that appear to called licensing factor, which is destroyed immediately affect the efficient function of ARSs [Maine et al. 1984; after replication initiation and which cannot gain access Gibson et al. 1990). In these mutants, the stability of minichromosomes is affected to dramatically different 1Present address: Department of Biology, University of California, San degrees, depending on which ARS is present on the min- Diego, California 92093 USA. ichromosome. These differences in stability suggest that GENES & DEVELOPMENT 7:2149-2160 9 1993by Cold Spring Harbor Laboratory Press ISSN 0890-9369/93 $5.00 2149 Downloaded from genesdev.cshlp.org on September 28, 2021 - Published by Cold Spring Harbor Laboratory Press Yan et al. the action of the MCM proteins are targeted at ARSs coincides with that of the hypothetical licensing factor (Maine et al. 1984; Gibson et al. 1990; Yan et al. 1991; in higher eukaryotes, that is, from nuclear envelope dis- Chen et al. 1992). In addition, in some of these mutants, sassembly to G~/S transition. such as mcm2-1, the instability of minichromosomes In this paper we show that defects in the MCM2 and has been shown to be the result of nonreplication rather MCM3 proteins lead to defects in the initiation of DNA than nondisjunction (Sinha et al. 1986). Although insta- synthesis at chromosomal replication origins. We show bility of plasmids is a general property of mutants defec- that the periodic nuclear import of these proteins occurs tive in DNA metabolism or chromosome segregation, with a timing that is synchronous with CDC46 and co- this ARS-specific destabilization of plasmids is unique incident with the cyclical breakdown of the nuclear en- only to the mcm mutants (Elble and Tye 1992). Other velope in higher eukaryotes. phenotypes associated with these mutants include in- creased rates of mitotic recombination and chromosome loss (Sinha et al. 1986; Gibson et al. 1990; Hennessy et Results al. 1990; Elble and Tye 1992), consistent with defects in MCM2 and MCM3 are required for the initiation DNA replication. of DNA synthesis at chromosomal replication origins Three of the MCM gene products, MCM2, MCM3, and MCM5, appear to be structurally and functionally re- The mcm mutants affect the stability of minichromo- lated. Sequence analysis revealed that MCM2 and somes in an ARS-specific manner. For example, mcm2-1 MCM3 share extensive amino acid sequence similarities destabilizes the minichromosome YCpl, which carries with each other (Yan et al. 1991) and with another yeast ARS1 but has no effect on the stability of YCpl21, which protein, CDC46, which is believed to play an important carries ARS121 (Fig. 1A). Similarly, mcm3-1 has a much role in the early steps of DNA replication (Hennessy et more dramatic effect on YCpl than YCpl21. This ARS- al. 1990, 1991). Complementation and genetic linkage dependent minichromosome maintenance defect of the analyses indicated that MCM5 is identical to CDC46 mcm mutants suggests that these mutants are defective (Chen et al. 1992). Sequence similarities within this pro- in the initiation of DNA synthesis at ARSs. To show tein family, designated MCM2-3-5, concentrate in three that this defect is not a phenomenon observed only in regions. The central and most extensive region contains plasmid replication, we examined the effect of the -60% identity and -80% similarity over -200 amino mcm2-1 and mcm3-1 mutations on the initiation of acids (Chen et al. 1992). Although these proteins are DNA synthesis at two chromosomal replication origins functionally and structurally related, they cannot func- by two-dimensional gel analysis (Brewer and Fangman tionally substitute one another. Each one of them is es- 1987). We chose to examine ORI1 (Ferguson et al. 1991) sential for cell growth (Yan et al. 1991). Homologs of and ORI121 (Walker et al. 1992), which corrrespond to MCM3 and CDC46/MCM5 have been identified in the native chromosomal locations of ARS 1 on chromo- other organisms. The fission yeast Schizosaccharomyces some IV and ARS 121 on chromosome X. pombe contains a CDC46/MCM5 homolog, Nda4, mu- Genomic DNAs were extracted from wild-type (8534- tations in which lead to nuclear division arrest (M. Yan- 8C), mcm2-1 (8534-M2), and mcm3-1 {R61-3C)mutant agida, pers. comm.). Mammalian cells contain an MCM3 strains that had been grown at 22~ and harvested at homolog, the P l protein, which was identified by early log phase. Although mcm2-1 has a reduced growth its copurification with the polymerase o~ holoenzyme rate (Maine et al. 1984) and mcm3-1 is conditional lethal (Thommes et al. 1992). Recently, Coxon et al. (1992) re- at 38~ (Fig. 2b; Gibson et al. 1990), both mutant strains ported that Cdc21 of S. pombe, which has an execution have a doubling time indistinguishable from that of the point before the hydroxyurea (HU) block, represents a wild-type strain at 22~ (Sinha et al. 1986; Gibson et al. new member of this family of proteins. It appears that 1990). Genomic DNA was digested either with NcoI, for this family of replication proteins may be conserved in the detection of replicating ORI1 DNA fragments, or all eukaryotes. with EcoRI-BamHI, for the detection of replicating The cdc46 mutants were originally identified as allele- ORI 121 DNA fragments. Restriction-digested DNA was specific suppressors of cdc45 and cdc54 mutants that subjected to two-dimensional agarose gel electrophore- arrest at the G1/S transition of the cell cycle (Moir et al. sis, which separates different topological forms of repli- 1982). Interestingly, cdc46 mutants also exhibit a G~/S cating DNA. Replicating DNA resulting from initiation arrest phenotype. Furthermore, like other mcm mutants, events that occurred within the ORI DNA fragments mi- cdc46/mcm5 mutants are defective in minichromosome grates with a characteristic pattern known as the "bub- maintenance in an ARS-specific manner (Chen et al. ble" arc (Fig. 1B, solid arrow). In contrast, replicating 1992).
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