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Eudragit® RL) Nanoparticles by Human THP-1 Cell Line and Its Effects on Hematology and Erythrocyte Damage in Rats

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Eudragit® RL) Nanoparticles by Human THP-1 Cell Line and Its Effects on Hematology and Erythrocyte Damage in Rats AVERTISSEMENT Ce document est le fruit d'un long travail approuvé par le jury de soutenance et mis à disposition de l'ensemble de la communauté universitaire élargie. Il est soumis à la propriété intellectuelle de l'auteur. Ceci implique une obligation de citation et de référencement lors de l’utilisation de ce document. D'autre part, toute contrefaçon, plagiat, reproduction illicite encourt une poursuite pénale. Contact : [email protected] LIENS Code de la Propriété Intellectuelle. articles L 122. 4 Code de la Propriété Intellectuelle. articles L 335.2- L 335.10 http://www.cfcopies.com/V2/leg/leg_droi.php http://www.culture.gouv.fr/culture/infos-pratiques/droits/protection.htm Ecole Doctorale BioSE (Biologie-Santé-Environnement) Thèse Présentée et soutenue publiquement pour l’obtention du titre de DOCTEUR DE l’UNIVERSITE DE LORRAINE Mention : « Sciences de la Vie et de la Santé » par Ramia SAFAR Etude toxicologique de nanoparticules polymériques véhicules de S-nitrosoglutathion Le 4 décembre 2015 Membres du jury : Rapporteurs : Mme Armelle BAEZA Professeur, Université Paris Diderot, Paris Mme Françoise PONS Professeur, Université de Strasbourg, Strasbourg Examinateurs : M. Bertrand RIHN Professeur, Université de Lorraine, Nancy Directeur de thèse M. Olivier JOUBERT Maître de conférences, Université de Lorraine, Nancy Co-directeur de thèse Invités : M. Jean-Claude ANDRE Directeur de Recherche Emérite, INSIS CNRS, Paris M. Alain LE FAOU Professeur Emérite, Université de Lorraine, Nancy EA 3452 CITHEFOR, « Cibles Thérapeutiques, Formulation et Expertise Pré-Clinique du Médicament » Faculté de Pharmacie, 5, rue Albert Lebrun, BP 80403, 54001 Nancy CEDEX 2 3 Je dédie ce manuscrit Aux mânes de mon père A ma mère A mes sœurs et mon frère A ma belle-famille, pour son soutien durant la réalisation de ce travail A ma petite famille, mon mari et mes enfants pour leur soutien et leur patience 4 5 Je voudrais remercier sincèrement, Mesdames les Professeures Françoise PONS et Armelle BAEZA pour avoir accepté de siéger dans mon jury et participé aux comités de suivi de thèse, ainsi pour leurs conseils et observations. Monsieur le Professeur Bertrand RIHN, mon directeur de thèse, pour l’expérience enrichissante et pleine d’intérêt qu’il m’a faite vivre durant ces trois ans. Monsieur le Docteur Olivier JOUBERT, Maître de Conférences, mon co-directeur de thèse, pour le suivi de mon travail, ses conseils, son enseignement. Monsieur le Professeur Emérite Alain LE FAOU, pour son aide à rédiger cette thèse et son soutien dans les moments difficiles. Monsieur le Directeur de Recherche Emérite Jean-Claude ANDRE pour avoir accepté de participer à mon jury. 6 7 Mes remerciements s’adressent aussi à mon pays et à la Faculté de Pharmacie (Université d’ALBAATH), qui m’ont donné l’opportunité et le soutien financier pour venir en France poursuivre mes études. En particulier, je tiens à remercier Madame la Professeure Laila MASSOUH pour son aide à trouver un laboratoire d’accueil pour ma thèse, et Monsieur le Professeur Houssam ALHUSAINI (Doyen de la Faculté de Pharmacie, Université d‘ALBAATH), pour le suivi de mon travail et ses conseils. Je remercie Messieurs les Professeurs Philippe MAINCENT et Pierre LEROY, directeurs de l’EA 3452 CITHEFOR pour m’avoir accueillie au sein de leur équipe. J’exprime également ma gratitude à l’égard de l’ensemble de l’équipe CITHEFOR pour leur gentillesse, leur sympathie qui ont favorisé mon intégration dans l’équipe. Je tiens à remercier tout particulièrement et à témoigner toute ma reconnaissance aux personnes suivantes : Monsieur le Pofesseur Luc FERRARI pour avoir participé au comité de suivi de thèse, ainsi que pour ses conseils. Dr.Anne SAPIN pour sa gentillesse et sa disponibilité. Dr.Housam EIDI pour son aide à mon arrivée en France et dans l’équipe. Dr.Carole RONZANI pour son aide pendant les deux premières années de ma thèse. Nathalie et Pascale, pour leur écoute, leur aide, leurs réponses à mes nombreuses questions. Je voudrais aussi remercier toutes les personnes qui ont participé de près ou de loin à mes travaux : Isabelle, Bouchra, Latifa, Razan, Emilie, Wen, Chloé, Laetitia, …. De plus j’adresse mes remerciements aux personnes qui ont collaboré à mon travail : Monsieur le Professeur Bernard Foliguet, directeur du Service de microscopie électronique, Faculté de médecine de Nancy. Dr Stéphanie GRANDEMANGE, UMR CNRS/UL 7039 CRAN, Nancy. Dr Christophe NEMOS, INSERM U 954, Nancy. Dr Virginie MARCHAND, Biopole, Faculté de médecine de Nancy. Dr Roudayna DIAB, UMR CNRS/UL 7565 SRSMC, Nancy. Dr Hélène DUBOIS-POT-SCHNEIDER, UMR CNRS/UL 7565 SRSMC, Nancy. 8 9 Travaux scientifiques Publications Acceptées Human monocyte response to S-nitrosoglutathione-loaded nanoparticles: uptake, viability, and transcriptome. R.SAFAR1, C.RONZANI1, R.DIAB, J.CHEVRIER, D.BENSOUSSAN, S.GRANDEMANGE, A.LE FAOU, B.H.RIHN, O.JOUBERT. Molecular Pharmaceutics, 2015 Jan 14. IF = 4.79 (1 co-premiers auteurs) Comment on: S-nitrosoglutathione (GSNO) is cytotoxic to intracellular amastigotes and promotes healing of topically treated Leishmania major or Leishmania braziliensis skin lesions C.RONZANI, R.SAFAR, A.LE FAOU, B.H.RIHN, O.JOUBERT. Journal of antimicrobial chemotherapy, 2014 Apr 28. IF = 5,33 Viability and gene expression responses to polymeric nanoparticles in human and rat cells C.RONZANI1 , R.SAFAR1 , R.DIAB, J.CHEVRIER, J.PAOLI, M.A.ABDEL-WAHHAB, A.LE FAOU, B.RIHN, O.JOUBERT. Cell Biology and Toxicology, 2014 Apr 21. IF = 2,33 (1 co-premiers auteurs) Uptake of Eudragit® Retard L (Eudragit® RL) Nanoparticles by Human THP-1 Cell Line and Its Effects on Hematology and Erythrocyte Damage in Rats. M.A.ABDEL-WAHHAB, K.G.ABDEL-WAHHAB, F.MANNAA, N.HASSAN, R.SAFAR, R.DIAB, B.FOLIGUET, L.FERRARI, B.H.RIHN. Materials, 2014 Fév 18. IF = 2,24 (Annexe 8) Role of the PLGA polymeric matrix composition on the cellular uptake and neuroprotective property of curcumin-loaded nanoparticle G.D.PAKA, S.DOGGUI, A.ZAGMI, R.SAFAR, L.DAO, A.KLYMCHENKO, G.ROULLIN, O.JOUBERT, C.RAMASSAMY. Molecular Pharmaceutics, IF = 4,79 (Annexe 9) Soumises Blood Compatibility of Multilayered Polyelectrolyte Films Containing Immobilized and Stabilized Gold Nanoparticles A.PALLOTTA, M.PARENT, M.LUO, I.CLAROT, R.SAFAR, O.JOUBERT, P.LEROY, A.BOUDIER. Advanced Healthcare Materials, IF = 5,79 10 En préparation Dose- and time- dependent responses of THP-1 cells exposed to free or encapsulated S- nitrosoglutathione. A transcriptomic comparison R.SAFAR, A.LE FAOU, C.NEMOS, H.DUBOIS-POT-SCHNEIDER, B.H.RIHN, O.JOUBERT. Eudragit® polymeric nanoparticles evaluation for the oral delivery of S-nitrosoglutathione 1 1 C.PUISNEY , R.SAFAR , W.WU, A.SAPIN, S.GRANDEMANGE, C.NEMOS, A.LE FAOU, BH. RIHN, O.JOUBERT, L.FERRARI 1 co-premiers auteurs Transcriptomes Transcriptome study of THP-1 human monocytes following exposure for 4 h or 24 h to 50 µM S-Nitrosoglutathione, 50 and 200 µg/ml S-Nitrosoglutathione-loaded polymeric and empty Eudragit® RL nanoparticles. C.RONZANI, R.SAFAR, B.RIHN, O.JOUBERT. GEO NCBI, 2013, accession number GSE51186 THP-1 human monocytes transcriptome following exposure to S-nitrosoglutatione (GSNO) R.SAFAR, C.PUISNEY, B.H.RIHN, O.JOUBERT. GEO NCBI, 2015, accession number GSE69024 Transcriptome study of Caco-2 human intestinal cells following exposure to empty or S- nitrosoglutathione-loaded polymeric nanoparticles C.PUISNEY, R.SAFAR, O.JOUBERT, L.FERRARI. GEO NCBI, 2015, accession number GSE69551 Communications orales* et affichées * Interaction cellules/nanoparticules polymériques : apport de la toxicogénomique R.SAFAR, C.RONZANI, A.LE FAOU, B.RIHN, L.FERRARI, O.JOUBERT 31èmes Journées Pédagogiques Et Scientifiques de l’AE2BM, 17-18 septemnbre 2015, Nancy * Cell / Polymeric Nanoparticle Interactions: Toxicogenomic Input. R.SAFAR, C.RONZANI, R.DIAB, A.LE FAOU, L.FERRARI, BH.RIHN, O.JOUBERT Congrès INRS “Risque chimique”, 8-10 avril 2015, Nancy. Cells / nanoparticles interactions: Toxicogenomics approach C.PUISNEY, R.SAFAR, BH.RIHN, C.NEMOS, O.JOUBERT, L.FERRARI Congrès INRS “Risque chimique”, 8-10 avril 2015, Nancy. In vitro study of cationized poly(caprolactone)-poly(ethylene glycol) nanoparticles C.CHAROONGCHIT, J.SUKSIRIWORAPONG, A.SAPIN, R.SAFAR, O.JOUBERT, B.RIHN, P.MAINCENT. 11 SFNano, 9-12 decembre 2014, Nancy Action of polymeric nanoparticles on cell viability, gene expression and internalization as a function of cell line R.SAFAR, C.RONZANI, R.DIAB, A.LE FAOU, BH.RIHN, O.JOUBERT The 50th Congress of the European Societies of Toxicology, Eurotox 2014, 7-10 sep 2014, Edimburg, Ecosse. (Bourses de congrès Eurotox et de la Société Française de Toxicologie) *Interaction cellules / Nanoparticules polymériques: apport de la toxicogénomique. C.RONZANI, R.SAFAR, R.DIAB, J.CHEVRIER, S.GRANDEMANGE, D.BENSOUSSAN, A.LE FAOU, BH.RIHN, O.JOUBERT Communication orale et affichée au congrès conjoint ARET/SFTG, 3 et 4 juin 2014, Paris. *Etude toxicologique de nanoparticules, véhicules de principe actif R.SAFAR, C.RONZANI, R.DIAB, S.GRANDEMANGE, B.FOLIGUET, A.LE FAOU, BH.RIHN, O.JOUBERT Journée scientifique de l’école doctorale BIOse, 6 novembre 2013, Nancy. *What about polymeric nanoparticles and mitochondria? BH.RIHN, R.SAFAR, C.RONZANI, R.HUSSIEN, G.A.BROOKS, A.LE FAOU, O.JOUBERT 5th International Symposium “Nutrition, Oxygen Biology and Medicine”, 5-7 juin 2013, Paris. Action of polymeric nanoparticles on cell viability, gene expression and internalization as a function of cell line R.SAFAR, C.RONZANI, S.GRANDEMANGE, B.FOLIGUET, BH.RIHN, O.JOUBERT 5th International
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