April 2011 Journal of Japanese Botany Vol. 86 No.2 115

J. Jpn. Bot. 86: 115–119 (2011)

a, b c d d Su-Juan Lin *, Takeshi Suzuki , Atsushi Ebihara , Haisheng Lu , Jianqiu Liu , e b Sugong Wu and Kunio Iwatsuki : A New Record of Polystichum otomasui Sa. Kurata (Dryopteridaceae) from Fujian Province, China aDepartment of Biological Science, Faculty of Life and Environmental Science, Shimane University, Nishikawatsu 1060, Matsue, Shimane, 690-8504 JAPAN bMuseum of Nature and Human Activities, Hyogo, 6, Yayoigaoka, Sanda, Hyogo, 669-1546 JAPAN cDepartment of Botany, National Museum of Nature and Science, 4-1-1, Amakubo, Tsukuba, 305-0005 JAPAN dCollege of Life Science, Fujian Normal University, Fuzhou, Fujian, 350108 P. R. CHINA eKunming Institute of Botany, Chinese Academy of Sciences, 132, Lanheilu, Kunming, Yunnan, 650204 P. R. CHINA *Corresponding author: [email protected]

Summary: Polystichum otomasui Sa. Kurata, which and humid floors along small streams, under had been regarded as an endemic fern to Japan, was mixed Phyllostachys heterocycla, Lithocarpus discovered in the Wuyi Mountains, Fujian Province, and Tsuga evergreen forests. The field research South East China. Cytological investigations area was further enlarged to Mt. Emeifeng (1714 confirmed that the new collections were sexual m alt., 27°03′N, 116°48′E), the southwestern diploids with a chromosome number of 2n = 82 (x = 41) and there were 64 spores per sporangium. These part of the Wuyi Mountains (Fig. 1) in 2008, morphological data (including spore perispores) where a big population (more than a hundred accorded with the characteristics of the Japanese individuals) was found on a path slope under the Polystichum otomasui. broadleaf evergreen forest. A comparative study was carried out The evergreen fern Polystichum otomasui between the Chinese collections and Japanese Sa. Kurata (Dryopteridaceae) was known as a Polystichum otomasui, and the specimens Japanese endemic, distributed in an extremely collected from the Wuyi Mountains were restricted area on humus-rich floor of mountain confirmed to be P. otomasui, which is a new forest in Kyusyu, Japan (Kurata 1963, Nakaike record in China (Fig. 2). 1992, Iwatsuki 1992, Mitsushima et al. 2009). In our field researches on “The comparative Morphology study of evergreen forests of Japan and Southern Polystichum otomasui is closely related to China” during 2006–2007, thirteen plants of P. makinoi (Tagawa) Tagawa. It was described Polystichum that resembled the Japanese P. as having shining blackish scales on stipes otomasui were collected from three populations and rachis; sori medial on pinnules of upper in Wuyishan National Nature Reserve, Fujian pinnae and marginal on auricles of pinnules province. One population with only several of lower pinnae or sometimes only one sorus plants was found on a light slope by a stream preferentially on each auricle at basal acroscopic under a secondary Lithocarpus forest of side of pinnules; indusia entire (Kurata 1963, Xianfengling at 1500 m altitude, and another Nakaike 1992, Iwatsuki et al. 1995). The two populations with more than ten individuals morphological characters of the plants from the each were found in an Old Tree Village (1100 m Wuyi Mountains accord with above mentioned alt.) and Tongmuguan (1700 m alt.) in the shady characters of Japanese P. otomasui (Fig. 2). 116 植物研究雑誌 第 86 巻 第 2 号 2011 年 4 月

Fig. 1. Map of Fujian Province, South East China. Dotted circle. Area of Fujian Wuyi Mountains. Solid square ( ■ ). Collection sites in China. Solid triangle ( ▲ ). Collection locality of Kumamoto Pref., Japan.

The fundamental similarities between the cytologically. All of them have the same somatic Chinese specimens and Japenese P. otomasui chromosome number of 2n = 82 (Fig. 4). In (from Kumamoto Pref., 071112-55) were addition, two samples (Lin & al. 060816-6, also confirmed by SEM observations of the 060816-9) were estimated to be diploid based on spores (Fig. 3). The spores of both samples are the genome size estimated by a flow cytometer. monolete, about 29 µm × 36 µm, ellipsoidal, All twenty samples collected from the Wuyi and echinate fenestrate and more or less cristae Mountains were counted to have 64 spores in perispore, although there are some more in each sporangium. Based on these results, or less discernable difference in the surface the Chinese samples were identified with P. appearance which could be explained as otomasui, a diploid sexual species. It was named intraspecific variation between populations due ‘Nanbi Erjue’ as Chinese name. to geographical isolation. Three related species, P. makinoi (Tagawa) Cytology Tagawa, P. acutipinnulum Ching & Shing, and The basic number of chromosomes in the P. wuyishanense Ching & Shing were recorded genus Polystichum was reported to be x = 41 in the vascular plant checklist of Wuyishan (Manton 1950, Daigobo 1973). Daigobo (1973) National Nature Reserve (Lin et al. 1993). reported that the Japanese P. otomasui was a Polystichum makinoi is a tetraploid species sexual diploid with a chromosome number of (Kurita 1966, Mitui 1968), morphologically n = 41. Two samples from Wuyishan Nature distinguished by lacking black scales on rachis, Reserve (Lin & al. 070814-1, 070814-2) and sori distributed on apical portion of laminae another two from Emeifeng mountain (Lin downwardly on auricles of pinnules in lower & al. 080812-42, 080812-50) were observed pinnae (Daigobo 1971, Iwatsuki et al. 1995), and April 2011 Journal of Japanese Botany Vol. 86 No.2 117

Fig. 2. Polystichum otomasui from Wuyishan National Nature Reserve, Fujian Province, China. spore about 50 µm × 60 µm (Lin unpublished). 0608Lin-1, 0608Lin-2, 0608Lin-5, 0608Lin-6, 0608Lin- However, P. acutipinnulum is a Chinese 9 (FNU, KUN, HYO, TNS). Tongmuguan, 1700 m alt., on a humid slope under a bamboo forest, 2007, Lin & al. endemic species, the type specimens were also 070814-1, 070814-2, 070814-36, 070814-37, 070814-38, collected from Tongmuguan, Wuyishan National 070814-39, 070814-46 (FNU and Herb. Faculty of Life and Nature Reserve (Ching 1981), is very similar Environmental Science, Shimane University, Japan). to P. otomasui morphologically, but different Taining Prefecture: Emeifeng mountain, 1714 m alt., in its falcate deltoid pinnules and indusia with on the way to Emeifeng Nature Reserve, on a path slope under broad leaf evergreen forest, 2008, Lin & al. 080812- irregular projections. Polystichum wuyishanense 4, 080812-7, 080812-11, 080812-13, 080812-20, 080812- has not been recognized as a distinct species, 21, 080812-33, 080812-39，080812-40, 080812-42, which was included in P. acutipinnulum by 080812-44, 080812-45, 080812-46, 080812-50 (FNU, and Kung et al. (2001). Herb. Faculty of Life and Environmental Science, Shimane University, Japan). Cytological and genetic information of the Chinese Polystichum is unsatisfactory for We would like to express our thanks to understanding the relationships among these curators of Wuyishan National Nature Reserve, species. Further systematic researches are Fujian Forestry Bureau for permitting our field expected. investigation on Wuyishan National Nature Voucher specimens from Fujian Province: Reserve. Wuyishan National Nature Reserve: on the way We thank Dr. A. Ohshima and Ms. M. from Tongmuguan to an Old Tree Village, 1100 m alt., Nagamoto for their technical assistance and help 2006, Lin & al. 060816-6 (FNU, KUN, HYO, TNS); near during field work. Thanks are also due to Dr. by the Observation Tower of Xianfengling Peak, 1500 m alt., and on the way to Sangang, on a humid & light slope Mei Sun for her useful comments and help with near a stream under a secondary forest, 2006, Lin & al. revising the manuscript. 118 植物研究雑誌 第 86 巻 第 2 号 2011 年 4 月

Fig. 3. SEM photos of spores, showing perispore sculpture. A, B. Sample from Mt. Emeifeng, Taining, Fujian Prov., China (Lin & al. 080812-13). C, D. Sample from Kumamoto Pref., Japan (Nagamoto & al. 07111255).

The curators of FNU, HUH, HYO, KUN, of the Ministry of Education, Culture, Sports, KYO, TI and TNS are acknowledged for their Science and Technology of Japan (project no. kind permission to examine specimens during 18405014 for K. Iwatsuki and no. 19570087 for this study, and the Department of Organismic S.-J. Lin). and Evolutionary Biology, Harvard University, is acknowledged for offering S.-J. Lin (the senior References author) comfortable research environment during Ching R. C., 1981. New ferns from Fujian Province (1). Wuyi Sci. J. 1: 1–12. her sabbatical leave period (2010). This study Daigobo S. 1971. Taxonomical studies on the fern genus was partly supported by the New Technology Polystichum in Japan, Ryukyu, and Taiwan. Sci. Rep. Development Foundation, and by Grants-in-Aid Tokyo Kyoiku Daigaku 15: 57–80. April 2011 Journal of Japanese Botany Vol. 86 No.2 119

Kurata S. and Nakaike T. 1983. Illustrations of Pteridophytes of Japan Volume 3. Tokyo University Press, Tokyo. Lin L. and Zhang Y. 1993. Vascular plants checklist of Wuyishan Nature Reserve. In: Zhao X. F., (ed.), The General Scientific Surveys of Wuyishan Nature Reserve. pp. 197–310. Fujian Science Press, Fuzhou. Manton I. 1950. Problems of Cytology and Evolution in the Pteridophyta. Cambridge University Press, New York. Mitsushima I., Tsuji H. and Takamiya M. 2009. Morphological, Cytological and molecular genetic studies of Polysticum otomasui Sa. Kurata found in Hita, Ooita Prefecture. Plant of Ooita Prefecture 19: 19–28 (in Japanese). Mitui K. 1968. Chromosomes and speciation in fern. Sci Rep. Tokyo Kyoiku Daigaku 13: 285–333. Nakaike T. 1992. New Fora of Japan. Pteridophyta. Fig. 4. Somatic chromosomes (2n = 82) of Polystichum Shibundo, Tokyo (in Japanese). otomasui (Lin & al. 070814-1) from Wuyishan National Nature Reserve, Fujian Prov., China. Bar = 10 µm. 林 蘇娟 a，鈴木 武 b，海老原 淳 c，盧 海声 d，劉 劍秋 d，武 素功 e，岩槻邦男 b：ナンピイノデの中国福 建省新分布記録 Daigobo S. 1973. Chromosome number of the fern genus 日本固有種とされてきたナンピイノデ Polystichum Polystichum (1). J. Jpn. Bot. 48: 337–344. Iwatsuki K. 1992. Ferns and Fern Allies of Japan. otomasui Sa. Kurata が中国の東南部福建省武夷山自然 Heibonsha, Tokyo (in Japanese). 保護区と武夷山脈南部の泰寧県峨嵋峰で発見された．染 Iwatsuki K., Yamazaki T., Boufford E. D. and Ohba H. 色体数 2n = 82（x = 41）であり，1 胞子嚢当り 64 胞 1995. Flora of Japan. Volume 1. Pteridophyta and 子性の有性生殖二倍体であることが確認された．形態 Gymnospermae. Kodansha, Tokyo. 学的な特徴（胞子，胞子の周皮模様を含む）は日本の Kung H. S., Zhang L. B., Zhu W. M., He Z. R. and Xie Y. Polystichum otomasui の特徴とよく一致した．ナンピ T. 2001. Dryopteridaceae (2). In: Kung H. S. (ed.), イノデには ｢南碧耳蕨｣ の中国名をつけた． Flora Reipublicae Popularis Sinicae. 5(2): 1–246. （a 島根大学生物資源科学部 , Science Press, Beijing (in Chinese). b 兵庫県立人と自然の博物館， Kurata S. 1963. Notes on Japanese ferns (32). J. Geobot. c 国立科学博物館植物研究部， 12: 66–69. d Kurita S. 1966. Chromosome numbers of some Japanese 中国福建師範大学生命科学学院， e ferns (5). J. Jpn. Bot. 41: 82–84. 中国科学院・昆明植物研究所）