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Probiotic Alternative to Chlorhexidine in Periodontal Therapy: Evaluation of Clinical and Microbiological Parameters
microorganisms Article Probiotic Alternative to Chlorhexidine in Periodontal Therapy: Evaluation of Clinical and Microbiological Parameters Andrea Butera , Simone Gallo * , Carolina Maiorani, Domenico Molino, Alessandro Chiesa, Camilla Preda, Francesca Esposito and Andrea Scribante * Section of Dentistry–Department of Clinical, Surgical, Diagnostic and Paediatric Sciences, University of Pavia, 27100 Pavia, Italy; [email protected] (A.B.); [email protected] (C.M.); [email protected] (D.M.); [email protected] (A.C.); [email protected] (C.P.); [email protected] (F.E.) * Correspondence: [email protected] (S.G.); [email protected] (A.S.) Abstract: Periodontitis consists of a progressive destruction of tooth-supporting tissues. Considering that probiotics are being proposed as a support to the gold standard treatment Scaling-and-Root- Planing (SRP), this study aims to assess two new formulations (toothpaste and chewing-gum). 60 patients were randomly assigned to three domiciliary hygiene treatments: Group 1 (SRP + chlorhexidine-based toothpaste) (control), Group 2 (SRP + probiotics-based toothpaste) and Group 3 (SRP + probiotics-based toothpaste + probiotics-based chewing-gum). At baseline (T0) and after 3 and 6 months (T1–T2), periodontal clinical parameters were recorded, along with microbiological ones by means of a commercial kit. As to the former, no significant differences were shown at T1 or T2, neither in controls for any index, nor in the experimental -
Microbiological, Lipid and Immunological Profiles in Children
Original Article http://dx.doi.org/10.1590/1678-77572016-0196 Microbiological, lipid and immunological SUR¿OHVLQFKLOGUHQZLWKJLQJLYLWLVDQG type 1 diabetes mellitus Abstract Cristiane DUQUE1 Objective: The aim of this study was to compare the prevalence of SHULRGRQWDOSDWKRJHQVV\VWHPLFLQÀDPPDWRU\PHGLDWRUVDQGOLSLGSUR¿OHVLQ Mariana Ferreira Dib JOÃO2 type 1 diabetes children (DM) with those observed in children without diabetes Gabriela Alessandra da Cruz (NDM), both with gingivitis. Material and methods: Twenty-four DM children Galhardo CAMARGO3 and twenty-seven NDM controls were evaluated. The periodontal status, 3 Gláucia Schuindt TEIXEIRA JO\FHPLF DQG OLSLG SUR¿OHV ZHUH GHWHUPLQHG IRU ERWK JURXSV 6XEJLQJLYDO Thamiris Santana MACHADO3 samples of periodontal sites were collected to determine the prevalence of Rebeca de Souza AZEVEDO3 SHULRGRQWDOPLFURRUJDQLVPVE\3&5%ORRGVDPSOHVZHUHFROOHFWHGIRU,/ǃ Flávia Sammartino MARIANO2 TNF-D and IL-6 analysis using ELISA kits. Results: Periodontal conditions of DM Natália Helena COLOMBO1 and NDM patients were similar, without statistical differences in periodontal indices. When considering patients with gingivitis, all lipid parameters Natália Leal VIZOTO2 evaluated were highest in the DM group; Capnocytophaga sputigena and Renata de Oliveira Capnocytophaga ochracea were more prevalent in the periodontal sites of DM 2 MATTOS-GRANER children. “Red complex” bacteria were detected in few sites of DM and NDM groups. Fusobacterium nucleatum and Campylobacter rectus were frequently IRXQGLQERWKJURXSV6LPLODUOHYHOVRI,/ǃ71)D -
Activity of Chlorhexidine Formulations on Oral Microorganisms and Periodontal Ligament Fibroblasts
Activity of chlorhexidine formulations on oral microorganisms and periodontal ligament fibroblasts Accepted for publication December 17, 2020 Alexandra Stähli1, Irina Liakhova1, Barbara Cvikl2, Adrian Lussi3, Anton Sculean1, Sigrun Eick1* 1Department of Periodontology, School of Dental Medicine, University of Bern, Switzerland 2Department of Conservative Dentistry, Sigmund Freud University, Vienna, Austria 3Department of Operative Dentistry and Periodontology, Faculty of Dentistry, University Medical Centre, Freiburg, Germany and School of Dental Medicine, University of Bern, Switzerland Keywords: biofilm; antiseptics; cytotoxicity Correspondence: Sigrun Eick, Klinik für Parodontologie, Labor Orale Mikrobiologie, Freiburgstrasse 7, CH-3010 Bern e-mail: [email protected]; phone +41 31 623 2542 1 Abstract Given the importance of microorganisms in the pathogenesis of the two most prevalent oral diseases (i.e. caries and periodontitis), antiseptics are widely used. Among the antiseptics chlorhexidine (CHX) is still considered as gold standard. The purpose of this in-vitro-study was to determine the antimicrobial activity of new CHX digluconate containing formulations produced in Switzerland. Two test formulations, with 0.1% or 0.2% CHX (TestCHX0.1, TestCHX0.2) were compared with 0.1% and 0.2% CHX digluconate solutions (CHXph0.1, CHXph0.2) without additives and with a commercially available formulation containing 0.2% CHX digluconate (CHXcom0.2). The minimal inhibitory concentrations (MIC) of the CHX formulations were determined against bacteria associated with caries or periodontal disease. Then the anti-biofilm activities of CHX preparations were tested regarding inhibition of biofilm formation or against an existing biofilm. Further, the cytotoxicity of the CHX preparations against periodontal ligament (PDL) fibroblasts was measured. There were no or only minor differences of the MIC values between the CHX preparations. -
Periodontal Health, Gingival Diseases and Conditions 99 Section 1 Periodontal Health
CHAPTER Periodontal Health, Gingival Diseases 6 and Conditions Section 1 Periodontal Health 99 Section 2 Dental Plaque-Induced Gingival Conditions 101 Classification of Plaque-Induced Gingivitis and Modifying Factors Plaque-Induced Gingivitis Modifying Factors of Plaque-Induced Gingivitis Drug-Influenced Gingival Enlargements Section 3 Non–Plaque-Induced Gingival Diseases 111 Description of Selected Disease Disorders Description of Selected Inflammatory and Immune Conditions and Lesions Section 4 Focus on Patients 117 Clinical Patient Care Ethical Dilemma Clinical Application. Examination of the gingiva is part of every patient visit. In this context, a thorough clinical and radiographic assessment of the patient’s gingival tissues provides the dental practitioner with invaluable diagnostic information that is critical to determining the health status of the gingiva. The dental hygienist is often the first member of the dental team to be able to detect the early signs of periodontal disease. In 2017, the American Academy of Periodontology (AAP) and the European Federation of Periodontology (EFP) developed a new worldwide classification scheme for periodontal and peri-implant diseases and conditions. Included in the new classification scheme is the category called “periodontal health, gingival diseases/conditions.” Therefore, this chapter will first review the parameters that define periodontal health. Appreciating what constitutes as periodontal health serves as the basis for the dental provider to have a stronger understanding of the different categories of gingival diseases and conditions that are commonly encountered in clinical practice. Learning Objectives • Define periodontal health and be able to describe the clinical features that are consistent with signs of periodontal health. • List the two major subdivisions of gingival disease as established by the American Academy of Periodontology and the European Federation of Periodontology. -
A Histologic Bioassay of the Effect of Endotoxin of Escherichia Coli 0111:B4 Strain Injected Into Guinea Pig Oral Mucosa
Loyola University Chicago Loyola eCommons Master's Theses Theses and Dissertations 1983 A Histologic Bioassay of the Effect of Endotoxin of Escherichia coli 0111:B4 Strain Injected Into Guinea Pig Oral Mucosa Juan Jose de Obarrio Loyola University Chicago Follow this and additional works at: https://ecommons.luc.edu/luc_theses Part of the Periodontics and Periodontology Commons Recommended Citation de Obarrio, Juan Jose, "A Histologic Bioassay of the Effect of Endotoxin of Escherichia coli 0111:B4 Strain Injected Into Guinea Pig Oral Mucosa" (1983). Master's Theses. 3280. https://ecommons.luc.edu/luc_theses/3280 This Thesis is brought to you for free and open access by the Theses and Dissertations at Loyola eCommons. It has been accepted for inclusion in Master's Theses by an authorized administrator of Loyola eCommons. For more information, please contact [email protected]. This work is licensed under a Creative Commons Attribution-Noncommercial-No Derivative Works 3.0 License. Copyright © 1983 Juan Jose de Obarrio A HISTOLOGIC BIOASSAY OF THE EFFECT OF ENDOTOXIN OF ESCHERICHIA COLI 0111:84 STRAIN INJECTED INTO GUINEA PIG ORAL MUCOSA BY Juan Jose de Obarrio A Thesis Submitted to the Faculty of the Graduate School of Loyola University of Chicago in Partial Fulfillment of the Requirements for the Degree of Master of Science May 1982 DEDICATION To my loving parents, Juan Luis and Helga, for their caring, understanding and who made possible my postgraduate education. To my fiance, Rocio, for her love and support. ii ACKNOWLEDGEMENTS I wish to express my sincere gratitude and appreciation to my Director, Dr. Patrick D. -
Suppression of Murine Lymphocyte Mitogen Responses by Exopolysaccharide from Capnocytophaga Ochracea RONALD W
INFECTION AND IMMUNITY, Jan. 1983, p. 476-479 Vol. 39, No. 1 0019-9567/83/010476-04$02.00/0 Copyright C 1983, American Society for Microbiology Suppression of Murine Lymphocyte Mitogen Responses by Exopolysaccharide from Capnocytophaga ochracea RONALD W. BOLTON* AND JOHN K. DYER Department of Oral Biology, College of Dentistry, University of Nebraska Medical Center, Lincoln, Nebraska 68583-0740 Received 26 July 1982/Accepted 5 October 1982 An extracellular polysaccharide was purified from culture supernatants of Capnocytophaga ochracea 25, a gram-negative bacillus associated with human periodontal disease. The extracellular polysaccharide suppressed in vitro mito- genic responses of murine splenic lymphocytes to concanavalin A and lipopoly- saccharide. This suppression was dose dependent, persisted up to 120 h, and was not caused by direct toxicity of the extracellular polysaccharide. Modulation of immune responses by bacterial C. ochracea 25, a gift from S. S. Socransky, components has received considerable attention Forsythe Dental Center, Boston, was cultivated in recent years (1, 2, 4, 5). Since a number of at 37°C in Trypticase soy broth supplemented these substances are produced by members of with 1% yeast extract and 0.1% NaHCO3. After the normal bacterial flora, they may have con- 48 h the cultures were centrifuged at 10,000 x g siderable impact on host-parasite interactions. for 15 min at 4°C. EP was isolated from liquid Indeed, there are several diseases of microbial culture supernatants by cold 95% ethanol pre- etiology, both clinical and experimental, which cipitation. The precipitate was dissolved in wa- have been associated with immune suppression ter and treated with cold 10% trichloroacetic or enhancement (reviewed in reference 12). -
Quantitative Molecular Detection of 19 Major Pathogens in the Interdental Biofilm of Periodontally Healthy Young Adults
fmicb-07-00840 May 31, 2016 Time: 12:58 # 1 ORIGINAL RESEARCH published: 02 June 2016 doi: 10.3389/fmicb.2016.00840 Quantitative Molecular Detection of 19 Major Pathogens in the Interdental Biofilm of Periodontally Healthy Young Adults Florence Carrouel1†, Stéphane Viennot2†, Julie Santamaria3, Philippe Veber4 and Denis Bourgeois2* 1 Institute of Functional Genomics of Lyon, UMR CNRS 5242, Ecole Normale Supérieure de Lyon, University Lyon 1, Lyon, France, 2 Laboratory “Health, Individual, Society” EA4129, University Lyon 1, Lyon, France, 3 Department of Prevention and Public Health, Faculty of Dentistry, University Lyon 1, Lyon, France, 4 Laboratory “Biométrie et Biologie Évolutive”, UMR CNRS 5558 – LBBE, University Lyon 1, Villeurbanne, France In oral health, the interdental spaces are a real ecological niche for which the body has few or no alternative defenses and where the traditional daily methods for control by disrupting biofilm are not adequate. The interdental spaces are Edited by: Yuji Morita, the source of many hypotheses regarding their potential associations with and/or Aichi Gakuin University, Japan causes of cardiovascular disease, diabetes, chronic kidney disease, degenerative Reviewed by: disease, and depression. This PCR study is the first to describe the interdental Kah Yan How, University of Malaya, Malaysia microbiota in healthy adults aged 18–35 years-old with reference to the Socransky Aurea Simón-Soro, complexes. The complexes tended to reflect microbial succession events in developing FISABIO Foundation, Spain dental biofilms. Early colonizers included members of the yellow, green, and Guliz N. Guncu, Hacettepe University, Turkey purple complexes. The orange complex bacteria generally appear after the early *Correspondence: colonizers and include many putative periodontal pathogens, such as Fusobacterium Denis Bourgeois nucleatum. -
Prevotella Intermedia
The principles of identification of oral anaerobic pathogens Dr. Edit Urbán © by author Department of Clinical Microbiology, Faculty of Medicine ESCMID Online University of Lecture Szeged, Hungary Library Oral Microbiological Ecology Portrait of Antonie van Leeuwenhoek (1632–1723) by Jan Verkolje Leeuwenhook in 1683-realized, that the film accumulated on the surface of the teeth contained diverse structural elements: bacteria Several hundred of different© bacteria,by author fungi and protozoans can live in the oral cavity When these organisms adhere to some surface they form an organizedESCMID mass called Online dental plaque Lecture or biofilm Library © by author ESCMID Online Lecture Library Gram-negative anaerobes Non-motile rods: Motile rods: Bacteriodaceae Selenomonas Prevotella Wolinella/Campylobacter Porphyromonas Treponema Bacteroides Mitsuokella Cocci: Veillonella Fusobacterium Leptotrichia © byCapnophyles: author Haemophilus A. actinomycetemcomitans ESCMID Online C. hominis, Lecture Eikenella Library Capnocytophaga Gram-positive anaerobes Rods: Cocci: Actinomyces Stomatococcus Propionibacterium Gemella Lactobacillus Peptostreptococcus Bifidobacterium Eubacterium Clostridium © by author Facultative: Streptococcus Rothia dentocariosa Micrococcus ESCMIDCorynebacterium Online LectureStaphylococcus Library © by author ESCMID Online Lecture Library Microbiology of periodontal disease The periodontium consist of gingiva, periodontial ligament, root cementerum and alveolar bone Bacteria cause virtually all forms of inflammatory -
The Biology of Marine Mammals
Romero, A. 2009. The Biology of Marine Mammals. The Biology of Marine Mammals Aldemaro Romero, Ph.D. Arkansas State University Jonesboro, AR 2009 2 INTRODUCTION Dear students, 3 Chapter 1 Introduction to Marine Mammals 1.1. Overture Humans have always been fascinated with marine mammals. These creatures have been the basis of mythical tales since Antiquity. For centuries naturalists classified them as fish. Today they are symbols of the environmental movement as well as the source of heated controversies: whether we are dealing with the clubbing pub seals in the Arctic or whaling by industrialized nations, marine mammals continue to be a hot issue in science, politics, economics, and ethics. But if we want to better understand these issues, we need to learn more about marine mammal biology. The problem is that, despite increased research efforts, only in the last two decades we have made significant progress in learning about these creatures. And yet, that knowledge is largely limited to a handful of species because they are either relatively easy to observe in nature or because they can be studied in captivity. Still, because of television documentaries, ‘coffee-table’ books, displays in many aquaria around the world, and a growing whale and dolphin watching industry, people believe that they have a certain familiarity with many species of marine mammals (for more on the relationship between humans and marine mammals such as whales, see Ellis 1991, Forestell 2002). As late as 2002, a new species of beaked whale was being reported (Delbout et al. 2002), in 2003 a new species of baleen whale was described (Wada et al. -
The Dental Office
GUIDED SURGERY • complete solutions for oral surgery the new generation digital platform iRES® SmartGuide® is the innovative and personalized solution for the entire clinical team-- dentistry that maintains strong ties with the dentistry of the future. iRES® offers dentists a valid system that meets all the patient’s requirements through adequate and personalized solutions planned and achieved using the vast, modern range of Cad Cam technologies with certified materials and executed with absolute excellence. The iRES® system guarantees the right solution for every need, from computer-assisted surgery with all the necessary required computer tools to all components for individual prostheses with 5 axes machine tools that carry out complex, individualized geometries with perfect results- -and all through only one source The dentist uses iRES® SmartGuide® software and with a few simple steps develops his or her own simple treatment plan, if necessary by combining his or her own requisites with our Tutor to achieve personalized assistance SmartGuide®: This is user-friendly software, a cost-contained, state-of- the-art system for quick, smooth ope- rating results—swift and non-traumatic surgery. iRES® offers a new surgery sy- stem using SmartGuide®. iRES® aims at furnishing the professional with an easy and intuitive system that provides both greater accuracy in positioning implan- ts and substantially reduced operating time, thus at once rendering the surgery as un-traumatic as possible. Costs are truly contained. iRES® delivers an overall system that includes: diagno- stic software and surgical/prosthetic planning, creation of the surgical mask, and the surgical kit including all drills ca- librated by diameter for all lengths. -
The Connection Between Socioeconomic Inequalities
Psychiatria Danubina, 2020; Vol. 32, Suppl. 4, pp S576-582 Medicina Academica Mostariensia, 2020; Vol. 8, No. 1-2, pp 178-184 Original paper © Medicinska naklada - Zagreb, Croatia THE CONNECTION BETWEEN SOCIOECONOMIC INEQUALITIES AND THE APPEARANCE OF THE TOOTHLESSNESS IN MOSTAR Bajro Sariü1, Kristina Galiü2,3, Belma Sariü Zolj3, Zdenko Šarac2, Marina ûurlin2 & Ivan Vasilj2 1Regional Medical Centre Mostar, Mostar, Bosnia and Herzegovina 2Medical School Mostar, Mostar, Bosnia and Herzegovina 3University Clinical Hospital Mostar, Mostar, Bosnia and Herzegovina received: 11.12.2019 revised: 7.5.2020 accepted: 7.7.2020 SUMMARY Background: To determine the existence of the toothlessness within the patients in the area of Mostar. The aim is to determine the topography of toothlessness within the population of Mostar, according to Kennedy classification. The aim is to connect measures of socioeconomic status with the appearance of the toothlessness. To develop a model that includes a form of toothlessness and the socioeconomic status of the patients in Mostar. Subjects and methods: The study was conducted at the Health Center in Mostar and the Regional Medical Center in Mostar. The research was cross-sectional study. It included 800 patients who regularlyoccurred to the dental ambulance because of the toothlessness and because of the prosthodontics treatment. The measurement was conducted by the dentist based on the anonymous research cardboard at the first examination of the patient. The dentist will determine the topography of the toothlessness according to Kennedy classification and the etiology of the toothlessness. Results: In the total sample of respondents, the toothlessness was significantly higher represented (P<0.001). -
Comparative Evaluation of Citrus Sinensis Extract on Porphyromonas
IOSR Journal of Dental and Medical Sciences (IOSR-JDMS) e-ISSN: 2279-0853, p-ISSN: 2279-0861.Volume 20, Issue 6 Ser.7 (June. 2021), PP 19-23 www.iosrjournals.org Comparative Evaluation of citrus sinensis extract on Porphyromonas Gingivalis, Aggregatibacter Actinomycetemcomitans and Prevotella Intermedia with Chlorhexidine: an In-Vitro Study 1 2 Dr.Nikee D. Upadhyay , Dr. Monali A. Shah 1(3rd MDS Periodontology, Department of Periodontology,K. M. Shah Dental College and Hospital, Sumandeep Vidyapeeth, Piparia, Vadodara, Gujarat,India) 2(Professor and Head,Department of Periodontology,K. M. Shah Dental College and Hospital, Sumandeep Vidyapeeth, Piparia, Vadodara, Gujarat,India) Abstract: Background: Periodontal pathogens like Aggregatibactor Actinomycetemcomitans, Porphyromonas Gingivalis, and Prevotella Intermedia etc. are considered to be the primary etiologic factors for the periodontal diseases. Chlorhexidine is an antimicrobial agent considered to be gold standard which has a broad antibacterial activity and is normally used for chemical plaque control. But chlorhexidine is known to cause staining when used for a longer time. Hence other agents with herbal contents are being researched that can be used on regular basis. Materials and Methods: Minimum inhibitory concentration (MIC), Minimum bactericidal concentration (MBC) and Zone of Inhibition (ZOI) were used to assess the antibacterial effect of citrus sinensis extract against periodontal pathogens and it was compared to chlorhexidine using the micro dilution process and the culture method. Results: For Citrus Sinensis extract, MIC and MBC value of P.Gingivalis was 50ug/ml and of A. Actinomycetemcomitans & P. Intermedia was 100ug/ml. ZOI of P.Gingivalis was 15mm at 50ug/ml, and A. actinomycetumcomitans and P.