Chryseobacterium Gleum Pneumonia in an Infant with Nephrotic Syndrome

IDCases 5 (2016) 34–36 Contents lists available at ScienceDirect IDCases journal homepage: www.elsevier.com/locate/idcr Case report Chryseobacterium gleum pneumonia in an infant with nephrotic syndrome Baha Abdalhamida,*, Nasreldin Elhadib, Khaldoon Alsammanb, Reem Aljindanc a Department of Pathology and Laboratory Medicine, King Fahad Specialist Hospital, P.O. Box 15215, Dammam, Saudi Arabia b Department of Clinical Laboratory Science, College of Applied Medical Science, University of Dammam, P.O. Box 2208, AlKhobar, Saudi Arabia c Department of Microbiology, College of Medicine, University of Dammam, P.O. Box 2208, AlKhobar, Saudi Arabia ARTICLE INFO ABSTRACT Article history: Introduction: Chryseobacterium gleum is commonly distributed in the environment. It can cause a wide Received 31 May 2016 variety of infections in immunocompromised patients in hospital setting. Received in revised form 21 June 2016 Case presentation: A 6 month old infant with nephrotic syndrome was admitted to the emergency room Accepted 27 June 2016 for an acute onset of fever, difficulty breathing, cyanosis, and low oral intake. Cultures of endotracheal tube specimens were positive for Chryseobacterium gleum which was confirmed by ribosomal Keywords: sequencing. The organism was susceptible to trimethoprim-sulfamethoxazole, minocycline, and Nephrotic syndrome levofloxacin. The patient clinically improved on levofloxacin treatment. Chryseobacterium gleum Conclusion: To the best of our knowledge, this is the first case of pneumonia caused by Chryseobacterium Levofloxacin fi Multiple drug resistance gleum in an infant with nephrotic syndrome. It is also the rst report of C. gleum causing respiratory tract infection in Saudi Arabia. ã 2016 The Authors. Published by Elsevier Ltd. This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/). Introduction spp are resistant to several antibiotics such as aminoglycosides, chloramphenicol, tetracyclines, clindamycin, teicoplanin, and Chryseobacterium species are widely distributed in the envi- erythromycin [1,10,11]. In addition, these strains chromosomally ronment. They cause infections in hospitalized patients with encode class A carbapenemases and class B metallo beta lactamases underlying conditions including immunocompromised patients which confer resistance to all b-lactams [12]. [1–3]. Age (infants, elderly) and medical devices such as mechanical ventilators or indwelling intravascular devices are Case report also common risk factors [1,4]. Chryseobacterium species, formerly known as Flavobacterium, A 6 month old infant brought to the emergency room in the belong to the family of Flavobacteriaceae [5]. CDC group IIb King Fahad Specialist Hospital-Dammam, Saudi Arabia with cough, comprises Chryseobacterium indolgenes and C. gleum and other fever, difficulty breathing, sneezing, irritation, excessive crying, strains [5]. They are aerobic, yellow pigmented on blood agar, decreased oral intake, and cyanosis. He had been diagnosed with catalase positive, oxidase positive, non motile, non glucose infantile nephrotic syndrome 5 months earlier. In his past medical fermenting Gram negative bacilli on MacConkey agar. These history, he had several episodes of septicemia caused mainly by strains can be differentiated based on DNA sequencing, several Pseudomonas aeruginosa. In addition, he was previously on phenotypic testing, and MALDI-TOF MS [5,6]. multiple courses of antibiotics including meropenem, ceftriaxone, Although less common than C. indolgenes,C. gleum has been and vancomycin. He was intubated and ventilated. Blood speci- reported to cause wide variety of infections including respiratory mens revealed mild neutrophilia and elevated CRP. Blood culture tract infections, urinary tract infections, pyonephrosis, septicemia, and urine culture were negative. Chest X rays revealed left sided meningitis, wound infections, and peritonitis [1,3,7–9]. Infections pleural effusion with left lower lobe opacities. Several respiratory caused by C. gleum had been reported in several countries including specimens from endotracheal tube (ETT) were submitted to the India, Hungary, Croatia,Qatar, and Taiwan [1,6–9]. Chryseobacterium microbiology laboratory for culture. Round yellow pigmented non hemolytic colonies grew on blood agar plates. Gram stain revealed Gram negative bacilli. The organism was catalase and oxidase * Corresponding author. positive. Organism identification and antimicrobial susceptibility E-mail address: [email protected] (B. Abdalhamid). http://dx.doi.org/10.1016/j.idcr.2016.06.004 2214-2509/ã 2016 The Authors. Published by Elsevier Ltd. This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/). B. Abdalhamid et al. / IDCases 5 (2016) 34–36 35 testing were carried out using the Vitek 2 automatic system no CLSI guidelines for Chryseobacterium [10,11]. According to (bioMerieux, Paris, France) according the manufacturer’s instruc- report from the SENTRYantimicrobial surveillance program (1997– tions. E. coli ATCC 25922 and Pseudomonas aeruginosa ATCC 2001), Chryseobacterium represented 0.27% of the studied non 27853 strains were used as controls for the antimicrobial fermentative Gram-negative rods and 0.03% of all bacterial isolates susceptibility testing. There are no Clinical and Laboratory [10]. Trimethoprim-sulfamethoxazole and newer generations of Standards Institute (CLSI) guidelines specific for Chryseobacterium quinolones (levofloxacin, garenoxacin, and gatifloxacin) were the species. However, minimum inhibitory concentrations (MIC) and most active agents against Chryseobacterium [10]. Our data breakpoints were determined according to the CLSI recommenda- correlated with this study where C. gleum strain was susceptible tion for other Non-Enterobacteriaceae. The organism was identi- to levofloxacin and trimethoprim-sulfamethoxazole. However, fied as Chryseobacterium gleum and it was confirmed using resistance to piperacillin-tazobactam and susceptibility to mino- API20NE kit and 16 S rRNA sequencing. The organism was resistant cycline in our study contradict data reported in the SENTRY study to ceftazidime (MIC 64 mg/mL), cefepime (MIC 64 mg/mL), regarding these two antibiotics [10]. meropenem (MIC 16 mg/mL), piperacillin-tazobactam (MIC There are discrepant data regarding Chryseobacterium suscep- 128 mg/mL), colistin (MIC 8 mg/mL), gentamicin (MIC 16 tibility to vancomycin. Several reports suggested susceptibility of mg/mL), and amikacin (MIC 64 mg/mL). It was intermediate to Chryseobacterium to vancomycin while other reports revealed imipenem (MIC = 8 mg/mL), ciprofloxacin (MIC = 2 mg/mL), and resistance to vancomycin [1,4,13]. In this study, the organism was tigecycline (MIC = 4 mg/mL). The organism was susceptible to resistant to vancomycin in vitro. trimethoprim-sulfamethoxazole (MIC 20 mg/mL), minocycline This report highlights the importance of Chryseobacterium (MIC 1 mg/mL), and levofloxacin (MIC = 0.5 mg/mL). In addition, species as a causing agent of the wide variety of infections. In the isolate was resistant to vancomycin. Environmental samples addition, it emphasizes the significance of establishing antimicro- from the mechanical ventilator and the patient room did not grow bial susceptibility testing guidelines for the genus any Chryseobacterium isolate. Chryseobacterium was tested for the Chryseobacterium. presence of carbapenem resistant genes (OXA48, NDM1, IMP, VIM, CTX-14, CTX-M15, and KPC) by multiplex PCR methodology using Conflict of interests ARM-D for b-Lactamase ID kit (Streck, Omaha, NE, USA) as instructed by the manufacturer. Positive controls and an internal The authors declare that they have no competing interests. control are included in the kit. In addition, molecular grade water (Promega, WI, USA) was used as a negative control to detect contamination. None of the tested genes was detected by PCR. The Authors’ contribution patient improved on levofloxacin for a period of 16 days and subsequent respiratory specimens did not grow C. gleum. Neither Baha Abdalhamid and Reem Aljindan reviewed the literature trimethoprim-sulfamethoxazole nor minocycline was used since and prepared the manuscript. Nasreldin Elhadi and Khaldoon they were not available in hospital at time of treatment. Alsamman performed the technical work. All authors approved the final version of the manuscript. Discussion Acknowledgment A case of pneumonia caused by C. gleum in an infant with nephrotic syndrome is reported in this study. To the best of our We would like to thank Streck Company in Omaha, NE, USA for knowledge, this is the first report of respiratory tract infection due providing the kit ARM-D for b-Lactamase ID free of charge. to C. gleum in Saudi Arabia. It is also the first report of association between Chryseobacterium infection and nephrotic syndrome. This References is a noteworthy case since Chryseobacterium species are ubiquitous in nature, are not part of human flora, and can cause infections in [1] Virok DP, Abrok M, Szel B, Tajti Z, Mader K, Urban E, et al. Chryseobacterium — hospitalized patients with underlying disease [1,2,4]. In addition, gleum a novel bacterium species detected in neonatal respiratory tract infections. J Matern-Fetal Neonatal Med 2014;27:1926–9. they are resistant to chlorination and are found on wet services of [2] Christakis GB, Perlorentzou SP, Chalkiopoulou I, Athanasiou A, Legakis NJ. medical devices and water systems. Therefore, medical devices Chryseobacterium

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