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Bruce Stewart Seal 2. Mailing Address: 19550 Sugar Mill Loop Bend, OR A. PERSONAL DATA 1. Full name: Bruce Stewart Seal 2. Mailing Address: 19550 Sugar Mill Loop Bend, OR 97702 Mobile Telephone: 1 (706) 540-4936 Email: [email protected] or [email protected] 3. Faculty rank: Adjunct and Instructor, Biology Program; Honors College Faculty Oregon State University, Cascades 1500 SW Chandler Avenue Bend, Oregon 97702 http://osucascades.edu/people/bruce-seal http://osucascades.edu/academics/biology Adjunct Graduate Faculty appointment (1996 – 2016) Department of Infectious Diseases College of Veterinary Medicine University of Georgia, Athens http://www.vet.uga.edu/ID B. SCHOLARLY COMPETENCE 1. Education: 1986 University of Nevada, Reno Ph.D. in Biochemistry Minor in Microbiology 1978 University of Nevada, Reno M.S. in Animal Physiology Minor in Biochemistry 1974 University of Nevada, Reno B.S. in Zoology 2. M.S. thesis title: The effect of boron in drinking water on the laboratory rat. 3. PhD dissertation title: Biochemical studies of bovine herpesvirus 1 DNA: Chromatin structure of infected cells, restriction endonuclease analysis of virus DNA and nucleic acid homology among isolates. 4. Academic and professional positions held: Dec 2014-present Adjunct and part-time Instructor, Biology Program and Honors College Faculty, Oregon State University Cascades campus, Bend OR 97701 USA 2004-2014 Supervisory Microbiologist/Research Leader, Poultry Microbiological Safety Research Unit, Russell Research Center, ARS, USDA, Athens, GA USA; NOTE: Personal Safety Level 2 (PSL2) Security Clearance for persons in positions of public trust for the U.S. federal government and BSL-3 working conditions 1993-2004 Microbiologist/Molecular Virology, Avian Paramyxovirus Project, Southeast Poultry Research Laboratory, USDA, Athens, Georgia (PSL-2 Clearance) 1991-1993 Microbiologist/Lead Scientist, Virology Swine Research, National Animal Disease Center, USDA, Ames, Iowa 1989-1991 Research Microbiologist, Virology Cattle Research, National Animal Disease Center, USDA, Ames, Iowa 1987-1989 Postdoctoral Fellow, Institute for Conservation Research, Zoological Society of San Diego, San Diego, California 1986-1987 Postdoctoral Research Fellow, Cancer Biology Program, Gildred Cancer Research Center, School of Medicine, University of California, San Diego, California 5. Publications a. Refereed articles: 1. Seal, B.S. and Weeth, H.J. The effect of boron in drinking water on the male laboratory rat. Bull. Environ. Contam. and Toxicol. 25:782-789, 1980. 2. Taylor, R.E.L., Seal, B.S. and St. Jeor, S.C. Isolation of infectious bovine rhinotracheitis virus from the soft-shelled tick, Ornithodoros coriaceus. Science. 216:300-301, 1982. 3. Seal, B.S., St. Jeor, S.C. and Taylor, R.E.L. Restriction endonuclease analysis of bovine herpesvirus 1 DNA and nucleic acid homology between isolates. J. Gen. Virol. 66:2787-2792, 1985. 4. Seal, B.S., Martinez, J.D., Hall, M.R. and St. Jeor, S.C. Occurrence of bovine herpesvirus-1 DNA in nucleosomes and chromatin of bovine herpesvirus-1 infected cells: Identification of a virion-associated protein in chromatin of infected cells. Arch. Virol. 99:221-236, 1988. 5. Seal, B.S. and St. Jeor, S.C. Purification and characterization of bovine herpesvirus-1 isolates and virus DNA utilizing bovine embryonic lung cells. J. Tissue Cult. Meth. 11:42-48, 1988. 6. Seal, B.S., Klieforth, R.B., Castro, A.E. and Heuschele, W.P. Replication of Alcelaphine herpesviruses in various cell culture systems and subsequent purification of virus. J. Tissue Cult. Meth. 11:49-56, 1988. 7. Seal, B.S., Klieforth, R.B., Welch, W.H. and Heuschele, W.P. Alcelaphine herpesviruses one and two: SDS-polyacrylamide gel electrophoresis analysis of virion polypeptides, restriction endonuclease analysis of genomic DNA and virus replication restriction in different cell types. Arch. Virol. 106:301-320, 1989. 8. Seal, B.S., Heuschele, W.P. and Klieforth, R.B. Prevalence of antibodies to alcelaphine herpesvirus-1 and nucleic acid hybridization analysis of viruses isolated from captive exotic ruminants. Am. J. Vet. Res. 50:1447-1453, 1989. 9. Seal, B.S., Klieforth, R.B. and Heuschele, W.P. Restriction endonuclease analysis of alcelaphine herpesvirus 1 DNA and molecular cloning of virus genomic DNA for potential diagnostic use. J. Vet. Diagn. Invest. 2:92-102, 1990. 10. MacLeod, C.L., Fong, A.M., Seal, B.S., Walls, L. and Wilkinson, M.F. Isolation of novel complementary DNA clones from T-lymphoma cells: One encodes a putative multiple membrane-spanning protein. Cell Growth Differ. 1:271-279, 1990. 11. Katz, J.B., Seal, B.S. and Ridpath, J.R. Molecular diagnosis of alcelaphine herpesvirus infection by nested amplification of viral specific DNA in bovine blood buffy coat B. Seal 3 specimens. J. Vet. Diagn. Invest. 3:193-198, 1991. 12. Seal, B.S., Irving, J.M., and Whetstone, C.A. Transcriptional analysis of the bovine herpesvirus 1 Cooper isolate: Temporal analysis and characterization of immediate-early, early and late RNA. Arch. Virol. 121:55-73, 1991. 13. Whetstone, C.A., Miller, J.M., Seal, B.S., Bello, L.J. and Lawrence, W.C. Latency and reactivation of a thymidine kinase-negative bovine herpesvirus I deletion mutant. Arch. Virol. 122:107-114, 1992. 14. Seal, B.S., Whetstone, C.A., Zamb, T., Bello, L.J., and Lawrence, W.C. Relationship of bovine herpesvirus 1 immediate-early, early and late gene expression to host cellular gene transcription. Virology. 188:152-159, 1992. 15. Whetstone, C.A., Seal, B.S. and Miller, J.M. Variability occurs in the inverted repeat region of genomic DNA from bovine herpesvirus 1 respiratory, genital and bovine herpesvirus 5 encephalitic isolates. Vet. Microbiol. 38:181-189, 1993. 16. Seal, B.S. and Whetstone, C.A. Immediate-early gene expression and gene mapping comparisons among isolates of bovine herpesvirus 1 and 5. Vet. Microbiol. 38:369-388, 1994. 17. Seal, B.S., Ridpath, J.F. and Mengeling, W.L. Analysis of feline calicivirus capsid protein genes: Identification of putative antigenic determinant regions of the protein. J. Gen. Virol. 74:2519-2524, 1993. 18. Kreutz, L.C., Seal, B.S. and Mengeling, W.L. Early interactions of feline calicivirus with cells in culture. Arch. Virol. 136:19-34, 1994. 19. Kleiboeker, S.B., Seal, B.S. and Mengeling, W.L Genomic cloning and restriction site mapping of a porcine adenovirus isolate: Demonstration of genomic stability in porcine adenovirus. Arch. Virol. 133:357-368, 1993. 20. Seal, B.S., Neill, J.D. and Ridpath, J.F. Predicted stem-loop structures and variation in nucleotide sequence of 3' non-coding regions among animal calicivirus genomic RNA. Virus Genes. 8:242-247, 1994. 21. Seal, B.S. Analysis of capsid protein gene variation among divergent isolates of feline calicivirus. Virus Res. 33:39-53, 1994. 22. Seal, B.S. and Neill, J.D. Capsid protein gene sequence of feline calicivirus isolates 255 and LLK. Further support for capsid protein configuration among feline caliciviruses. Virus Genes.9:183-187, 1995. 23. Kreutz, L.C. and Seal, B.S. The pathway of feline calicivirus entry. Virus Res. 35:63-70, 1995. 24. Neill, J.D. and Seal, B.S. Development of PCR primers for specific amplification of two distinct regions of the genomes of San Miguel sea lion virus and vesicular exanthema of swine virus. Mol. Cell. Probes.9:33-37, 1995. 25. Seal, B.S., House, J.A., Whetstone, C.A. and Neill, J.D. Further analysis of the serological relationship among San Miguel sea lion virus and vesicular exanthema of swine virus: Development of a Western blot assay for detection of antibodies in swine sera to these virus types. J. Vet. Diagn. Invest. 7:190-195, 1995. 26. Neill, J.D., Meyer, R.F. and Seal, B.S. Genetic relatedness of the caliciviruses: San Miguel sea lion and vesicular exanthema of swine viruses constitute a single genotype within the Caliciviridae. J. Virol. 69:4484-4488, 1995. 27. Seal, B.S., Lutze-Wallace, C., Sapp, T., Dulac, G., Kreutz, L.C. and Neill, J.D. Isolation and characterization of caliciviruses from skunks antigenically and genotypically related to San Miguel sea lion virus. Virus Res. 37:1-12, 1995. 28. Seal, B.S., King, D.J. and Bennett, J.D. Characterization of Newcastle disease virus isolates by reverse transcription PCR coupled to direct nucleotide sequencing and B. Seal 4 development of a sequence database for pathotype prediction and molecular epidemiological analysis. J. Clin. Microbiol. 33:2624-2630, 1995. 29. Marin, M.C., Villegas, P., Bennett, J. and Seal, B. Virus characterization and sequence of the fusion protein gene cleavage site of recent Newcastle disease virus field isolates. Avian Dis. 40:382-390, 1996. 30. Seal, B.S. Analysis of matrix protein gene nucleotide sequence diversity among Newcastle disease virus isolates demonstrates that recent disease outbreaks are caused by viruses of psittacine origin. Virus Genes. 11:217-224, 1996. 31. Seal, B.S., King, D.J. and Bennett, J.D. Characterization of Newcastle disease virus vaccines by biological properties and sequence analysis of the hemagglutinin- neuraminidase protein gene. Vaccine. 14:761-766, 1996. 32. King, D.J. and Seal, B.S. Biological and molecular characterization of Newcastle disease virus isolates from surveillance of live bird markets in the Northeastern United States. Avian Dis. 41:683-689, 1997. 33. Neill, J.D., Meyer, R.F. and Seal, B.S. The capsid protein gene of vesicular exanthema of swine virus serotype A48: relationship to the capsid protein of San Miguel sea lion virus. Virus Res. 54:39-50, 1998. 34. Seal, B.S., King, D.J., Locke, D.P., Senne, D.A. and Jackwood, M.W. Phylogenetic relationships among highly virulent Newcastle disease virus isolates obtained from exotic birds and poultry from 1989 to 1996. J. Clin. Micro. 36:1141-1145, 1998. 35. Kreutz, L.C., Johnson, R.P. and Seal, B.S.
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