Musculoskeletal System
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Mesenchymal Stem Cells in Combination with Hyaluronic Acid
www.nature.com/scientificreports OPEN Mesenchymal Stem Cells in Combination with Hyaluronic Acid for Articular Cartilage Defects Received: 1 August 2017 Lang Li1, Xin Duan1, Zhaoxin Fan2, Long Chen1,3, Fei Xing1, Zhao Xu4, Qiang Chen2,5 & Accepted: 19 April 2018 Zhou Xiang1 Published: xx xx xxxx Mesenchymal stem cells (MSCs) and hyaluronic acid (HA) have been found in previous studies to have great potential for medical use. This study aimed to investigate the therapeutic efects of bone marrow mesenchymal stem cells (BMSCs) combined with HA on articular cartilage repair in canines. Twenty-four healthy canines (48 knee-joints), male or female with weight ranging from 5 to 6 kg, were operated on to induce cartilage defect model and divided into 3 groups randomly which received diferent treatments: BMSCs plus HA (BMSCs-HA), HA alone, and saline. Twenty-eight weeks after treatment, all canines were sacrifced and analyzed by gross appearance, magnetic resonance imaging (MRI), hematoxylin-eosin (HE) staining, Masson staining, toluidine blue staining, type II collagen immunohistochemistry, gross grading scale and histological scores. MSCs plus HA regenerated more cartilage-like tissue than did HA alone or saline. According to the macroscopic evaluation and histological assessment score, treatment with MSCs plus HA also lead to signifcant improvement in cartilage defects compared to those in the other 2 treatment groups (P < 0.05). These fndings suggested that allogeneic BMSCs plus HA rather than HA alone was efective in promoting the formation of cartilage-like tissue for repairing cartilage defect in canines. Articular cartilage is composed of chondrocyte and extracellular matrix and has an important role in joint move- ment including lubrication, shock absorption and conduction. -
(AMIC) Compared to Microfractures for Chondral Defects of the Talar Shoulder: a Five-Year Follow-Up Prospective Cohort Study
life Communication Autologous Matrix Induced Chondrogenesis (AMIC) Compared to Microfractures for Chondral Defects of the Talar Shoulder: A Five-Year Follow-Up Prospective Cohort Study Filippo Migliorini 1 , Jörg Eschweiler 1, Nicola Maffulli 2,3,4,5,* , Hanno Schenker 1, Arne Driessen 1 , Björn Rath 1,6 and Markus Tingart 1 1 Department of Orthopedics and Trauma Surgery, University Clinic Aachen, RWTH Aachen University Clinic, 52064 Aachen, Germany; [email protected] (F.M.); [email protected] (J.E.); [email protected] (H.S.); [email protected] (A.D.); [email protected] (B.R.); [email protected] (M.T.) 2 School of Pharmacy and Bioengineering, Keele University School of Medicine, Staffordshire ST4 7QB, UK 3 Barts and the London School of Medicine and Dentistry, London E1 2AD, UK 4 Centre for Sports and Exercise Medicine, Queen Mary University of London, Mile End Hospital, London E1 4DG, UK 5 Department of Orthopedics, Klinikum Wels-Grieskirchen, A-4600 Wels, Austria 6 Department of Medicine, Surgery and Dentistry, University of Salerno, 84081 Baronissi, Italy * Correspondence: [email protected] Abstract: Introduction: Many procedures are available to manage cartilage defects of the talus, Citation: Migliorini, F.; Eschweiler, J.; including microfracturing (MFx) and Autologous Matrix Induced Chondrogenesis (AMIC). Whether Maffulli, N.; Schenker, H.; Driessen, AMIC or MFx are equivalent for borderline sized defects of the talar shoulder is unclear. Thus, the A.; Rath, B.; Tingart, M. Autologous present study compared the efficacy of primary isolated AMIC versus MFx for borderline sized Matrix Induced Chondrogenesis focal unipolar chondral defects of the talar shoulder at midterm follow-up. -
Series 1100TDM Tandem MEGALUG Mechanical Joint Restraint
Series 1100TDM Tandem MEGALUG® Mechanical Joint Restraint High Pressure Restraint for Ductile Iron Pipe Features and Applications: • For use on Ductile Iron Pipe 4 inch through 54 inch • High Pressure Restraint • Torque Limiting Twist-Off Nuts • Mechanical Joint follower gland incorporated into the restraint • MEGA-BOND® Coating System For more information on MEGA- BOND, visit our web site at www. ebaa.com • Minimum 2 to 1 Safety Factor Series 1112TDM restraining a mechanical joint fitting. • Constructed of A536 Ductile Iron Post Pressure Rating • EBAA-Seal™ Mechanical Nominal Pipe Shipping Assembly (PSI) Joint Gaskets are provided Size Weights* Deflection with all 1100TDM MEGALUG 4 21.6 3° 700 restraints. These are required 6 33.0 3° 700 to accommodate the pressure ratings and safety factors 8 40.0 3° 700 shown. 10 60.2 3° 700 12 75.0 3° 700 • New: High strength heavy hex 14 112.7 2° 700 machine bolts with T-nuts are 16 131.6 2° 700 provided to facilitate easier assembly due to the fittings 18 145.2 1½° 500 radius area prohibiting the use 20 166.6 1½° 500 longer T-bolts. 24 290.2 1½° 500 30 457.9 1° 500 • T-Nuts constructed of High 36 553.63 1° 500 Tensile Ductile Iron with Fluropolymer Coating. 42 1,074.8 1° 500 48 1,283.1 1° 500 For use on water or wastewater 54 1,445.32 ½° 400 pipelines subject to hydrostatic NOTE: For applications or pressures other than those shown please pressure and tested in accordance contact EBAA for assistance. -
Anatomy and Physiology
Anatomy and Physiology By Dr. Marwan Arbilei SYSTEMS INSIDE THE BODY What Is Anatomy and Physiology? • Skeletal system • Muscular system • Anatomy is the study of the • Cardiovascular system structure and relationship • Digestive system between body parts. • Endocrine system • Nervous system • Physiology is the study of the • Respiratory system function of body parts and • Immune/ Lymphatic system the body as a whole. • Urinary system • Male and Female Reproductive system • Integumentary system Skeletal system The axial skeleton runs along the body’s midline axis and is made up of 80 bones in the following regions: Skull Hyoid Auditory ossicles Ribs Sternum Vertebral column The appendicular skeleton is made up of 126 bones in the following regions: Upper limbs Lower limbs Pelvic girdle Pectoral (shoulder) girdle Joints Fibrous Joint -non movable. eg: skull Cartilaginous Joint –chest bone, vertebrae Synovial Joint – elbow,knee,hip,shoulder,finger Vertebral column • Vertebral column • Total 33 vertebrae • Cervical 7 • Thoracic 12 • Lumber 5 • Sacral 5 • Coccygeial 4 Muscular system There are three types of muscle tissue: Visceral Stomach, intestines, blood vessels Cardiac Heart Skeletal Muscles attached to two bones across a joint Cardiovascular system Anatomy • The Heart • Circulatory Loops Functions • Blood Vessels Transportation • Coronary Circulation Protection • Hepatic Portal Circulation Regulation • Blood Digestive system Anatomy Mouth-Pharynx – Esophagus – Stomach - Small Intestine - Liver and Gallbladder – Pancreas -
Applications of Chondrocyte-Based Cartilage Engineering: an Overview
Hindawi Publishing Corporation BioMed Research International Volume 2016, Article ID 1879837, 17 pages http://dx.doi.org/10.1155/2016/1879837 Review Article Applications of Chondrocyte-Based Cartilage Engineering: An Overview Abdul-Rehman Phull,1 Seong-Hui Eo,1 Qamar Abbas,1 Madiha Ahmed,2 and Song Ja Kim1 1 Department of Biological Sciences, College of Natural Sciences, Kongju National University, Gongjudaehakro 56, Gongju 32588, Republic of Korea 2Department of Pharmacy, Quaid-i-Azam University, Islamabad 45320, Pakistan Correspondence should be addressed to Song Ja Kim; [email protected] Received 14 May 2016; Revised 24 June 2016; Accepted 26 June 2016 Academic Editor: Magali Cucchiarini Copyright © 2016 Abdul-Rehman Phull et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. Chondrocytes are the exclusive cells residing in cartilage and maintain the functionality of cartilage tissue. Series of biocomponents such as different growth factors, cytokines, and transcriptional factors regulate the mesenchymal stem cells (MSCs) differentiation to chondrocytes. The number of chondrocytes and dedifferentiation are the key limitations in subsequent clinical application of the chondrocytes. Different culture methods are being developed to overcome such issues. Using tissue engineering and cell based approaches, chondrocytes offer prominent therapeutic option specifically in orthopedics for cartilage repair and to treat ailments such as tracheal defects, facial reconstruction, and urinary incontinence. Matrix-assisted autologous chondrocyte transplantation/implantation is an improved version of traditional autologous chondrocyte transplantation (ACT) method. An increasing number of studies show the clinical significance of this technique for the chondral lesions treatment. -
Research Review Fibrocartilage
J. Anat. (1990), 171, pp. 1-15 1 Printed in Great Britain Research Review Fibrocartilage M. BENJAMIN AND E. J. EVANS Department of Anatomy, University of Wales College of Cardiff, PO Box 900, Cardif CF1 3 YF, Wales Fibrocartilage has long been a neglected tissue that is too often viewed as a poor relation of hyaline cartilage. It failed to achieve the status of a tissue with the early histologists, but it is beginning to come of age, for modem techniques are revealing some exciting secrets about fibrocartilage in knee joint menisci and intervertebral discs in particular. Yet there has never been any general review on fibrocartilage, and workers concerned with the tissue in one organ rarely consider it in another. Consequently, we lack any global picture that would encourage the spread of interest in the tissue and the effective exchange of ideas. Our review deals largely with the white fibrocartilage of standard texts and for reasons of space excludes yellow elastic cartilage. We have concentrated on fibrocartilage as a tissue rather than fibrocartilages as organs. HISTORICAL CONSIDERATIONS The most important work on cartilage in the older literature is that of Schaffer (1930). His monograph is a thorough, comparative account of cartilage and related tissues throughout the animal kingdom. The reader interested in fibrocartilage must also study Schaffer's account of chondroid tissue, for some tissues that would now be regarded as fibrocartilage were viewed by Schaffer as hyaline-cell chondroid tissue. He had a narrow vision of 'true' cartilage and called tissues where the cells were not shrunken in lacunae, 'chondroid'. -
Autologous Matrix-Induced Chondrogenesis and Generational Development of Autologous Chondrocyte Implantation
Autologous Matrix-Induced Chondrogenesis and Generational Development of Autologous Chondrocyte Implantation Hajo Thermann, MD, PhD,* Christoph Becher, MD,† Francesca Vannini, MD, PhD,‡ and Sandro Giannini, MD‡ The treatment of osteochondral defects of the talus is still controversial. Matrix-guided treatment options for covering of the defect with a scaffold have gained increasing popularity. Cellular-based autologous chondrocyte implantation (ACI) has undergone a generational development overcoming the surgical drawbacks related to the use of the periosteal flap over time. As ACI is associated with high costs and limited in availability, autologous matrix-induced chondrogenesis, a single-step procedure combining microfracturing of the subchondral bone to release bone marrow mesenchymal stem cells in combination with the coverage of an acellular matrix, has gained increasing popularity. The purposes of this report are to present the arthroscopic approach of the matrix-guided autologous matrix-induced chondrogenesis technique and generational development of ACI in the treatment of chondral and osteochon- dral defects of the talus. Oper Tech Orthop 24:210-215 C 2014 Elsevier Inc. All rights reserved. KEYWORDS cartilage, defect, ankle, talus, AMIC, ACI Introduction Cartilage repair may be obtained by cartilage replacement: (OATS, mosaicplasty) or with techniques aimed to generate a hondral and osteochondral lesions are defects of the newly formed cartilage such as microfracture or autologous Ccartilaginous surface and underlying subchondral bone of chondrocyte implantation (ACI).9-17 the talar dome. These defects are often caused by a single or Arthroscopic debridement and bone marrow stimulation multiple traumatic events, mostly inversion or eversion ankle using the microfracture technique has proven to be an 1,2 sprains in young, active patients. -
Chapter 14. Anthropometry and Biomechanics
Table of contents 14 Anthropometry and biomechanics........................................................................................ 14-1 14.1 General application of anthropometric and biomechanic data .....................................14-2 14.1.1 User population......................................................................................................14-2 14.1.2 Using design limits ................................................................................................14-4 14.1.3 Avoiding pitfalls in applying anthropometric data ................................................14-6 14.1.4 Solving a complex sequence of design problems ..................................................14-7 14.1.5 Use of distribution and correlation data...............................................................14-11 14.2 Anthropometric variability factors..............................................................................14-13 14.3 Anthropometric and biomechanics data......................................................................14-13 14.3.1 Data usage............................................................................................................14-13 14.3.2 Static body characteristics....................................................................................14-14 14.3.3 Dynamic (mobile) body characteristics ...............................................................14-28 14.3.3.1 Range of whole body motion........................................................................14-28 -
Module 2 : Anatomy – the Skeleton
Module 2 : Anatomy – The Skeleton In this module you will learn: The functions of the skeletal system The types of bones in the human body The effects of exercise on your bones What happens to the bones as we get older When studying to become a fitness instructor or personal trainer, you will learn all about the anatomy of the human body. Studying the skeleton is one of the foundations of your trade, you will need to know how the body is structured, the names of each bone, types of bones, importance of bone and joint health, detail of the spine and different terms of movement. Without stating the obvious, each of your clients has their own skeleton and you must be fully aware of how this works. This is for many reasons; you are a teacher and must be fully aware of how to prevent injuries, avoid unnecessary stress on the bones and, if qualified, help the client prevent or heal bone and joint related conditions or medical problems. 2.1 Understanding the Skeletal System The skeleton is comprised of 206 different bones that provide 5 main functions: Support mechanism for muscle and tissue Protection for organs Movement with bones, muscles, and joints Storing minerals and blood cells Growth Skeletal System 2.2 Bones are Formed by Ossification Some bones (such as the flat bones of your skull) in the body are formed in a similar stage to connective tissue. The process is known as direct or intramembranous ossification. Other bones are made up of cartilaginous matter, this is developed from future bone in the embryo which then dissolves and is replaced with other bone cells. -
Internet Research Sites
Human Body Project Helpful Sites General Sites www.exploratorium.edu http://www.ehc.com/vbody.asp A virtual body tour (brain, skeleton, heart, and digestive tract). http://www.kidsbiology.com/human_biology/index.php A kid-friendly site that allows you to explore your body under one of the human body systems. Ophthalmologists- Muscular System http://www.exploratorium.edu/learning_studio/cow_eye/coweye.pdf Step by step directions on cow eye dissection. http://www.exploratorium.edu/learning_studio/cow_eye/ -Cow eye dissection video. Cardiologists- Circulatory System Cardiovascular System Circulatory System Circulatory System 1 Circulatory System: The Life Pump How Your Heart Works Kids Health: Your Heart & Circulatory System Preview the Heart The Circulatory System The Human Heart Wikipedia: The Circulatory System Orthopedic Specialist-Skeletal System http://kidshealth.org/kid/htbw/bones.html Multi-media Flash videos and information on bones. eSkeletons Project Human Skeleton Printout-Enchanted Learning.com Kids Health: Your Bones Skeletal System Skeletal System: The Bone Zone Skeletal System (Front View) Skeletal System (Back View) The Skeletal System The Skeleton Wikipedia: Skeleton Pulmonary Specialist- Respiratory System http://kidshealth.org/kid/closet/movies/asthma_movie.html Flash video showing Asthma’s effect on our lungs. Air Bags: The Respiratory System How the Body Works: The Respiratory System Kids Health: Your Lungs & Respiratory System Oxygen Delivery System Respiratory System The Respiratory System Your Respiratory System -
38.3 Joints and Skeletal Movement.Pdf
1198 Chapter 38 | The Musculoskeletal System Decalcification of Bones Question: What effect does the removal of calcium and collagen have on bone structure? Background: Conduct a literature search on the role of calcium and collagen in maintaining bone structure. Conduct a literature search on diseases in which bone structure is compromised. Hypothesis: Develop a hypothesis that states predictions of the flexibility, strength, and mass of bones that have had the calcium and collagen components removed. Develop a hypothesis regarding the attempt to add calcium back to decalcified bones. Test the hypothesis: Test the prediction by removing calcium from chicken bones by placing them in a jar of vinegar for seven days. Test the hypothesis regarding adding calcium back to decalcified bone by placing the decalcified chicken bones into a jar of water with calcium supplements added. Test the prediction by denaturing the collagen from the bones by baking them at 250°C for three hours. Analyze the data: Create a table showing the changes in bone flexibility, strength, and mass in the three different environments. Report the results: Under which conditions was the bone most flexible? Under which conditions was the bone the strongest? Draw a conclusion: Did the results support or refute the hypothesis? How do the results observed in this experiment correspond to diseases that destroy bone tissue? 38.3 | Joints and Skeletal Movement By the end of this section, you will be able to do the following: • Classify the different types of joints on the basis of structure • Explain the role of joints in skeletal movement The point at which two or more bones meet is called a joint, or articulation. -
Musculoskeletal Morphing from Human to Mouse
Procedia IUTAM Procedia IUTAM 00 (2011) 1–9 2011 Symposium on Human Body Dynamics Musculoskeletal Morphing from Human to Mouse Yoshihiko Nakamuraa,∗, Yosuke Ikegamia, Akihiro Yoshimatsua, Ko Ayusawaa, Hirotaka Imagawaa, and Satoshi Ootab aDepartment of Mechano-Informatics, Graduate School of Information and Science and Technology, University of Tokyo, 7-3-1, Hongo, Bunkyo-ku, Tokyo, Japan bBioresource Center, Riken, 3-1-1 Takanodai, Tsukuba-shi, Ibaragi, Japan Abstract The analysis of movement provides various insights of human body such as biomechanical property of muscles, function of neural systems, physiology of sensory-motor system, skills of athletic movements, and more. Biomechan- ical modeling and robotics computation have been integrated to extend the applications of musculoskeletal analysis of human movements. The analysis would also provide valuable means for the other mammalian animals. One of current approaches of post-genomic research focuses to find connections between the phenotype and the genotype. The former means the visible morphological or behavioral expression of an animal, while the latter implies its genetic expression. Knockout mice allows to study the developmental pathway from the genetic disorders to the behavioral disorders. Would musculoskeletal analysis of mice also offer scientific means for such study? This paper reports our recent technological development to build the musculoskeletal model of a laboratory mouse. We propose mapping the musculoskeletal model of human to a laboratory mouse based on the morphological similarity between the two mammals. Although the model will need fine adjustment based on the CT data or else, we can still use the mapped musculoskeletal model as an approximate model of the mouse’s musculoskeletal system.