Product datasheet Anti-CAMKK2 Antibody Catalog Number: BA3629

BOSTER BIOLOGICAL TECHNOLOGY Special NO.1, International Enterprise Center, 2nd Guanshan Road, Wuhan, China

Web: www.boster.com.cn Phone: +86 27 67845390 Fax: +86 27 67845390 Email: [email protected]

Basic Information Product Name Anti-CAMKK2 Antibody Name CAMKK2 Source Rabbit IgG Species Reactivity human,mouse,rat Tested Application WB,IHC-P,ICC Contents 500ug/ml antibody with PBS ，0.02% NaN3 , 1mg BSA and 50% glycerol. Immunogen A synthetic peptide corresponding to a sequence in the middle region of human CAMKK2(180-194aa KLAYNENDNTYYAMK), identical to the related rat and mouse sequences. Purification Immunogen affinity purified. Observed MW 65KD Dilution Ratios Western blot: 1:500-2000 Immunohistochemistry(Paraffin-embedded Section): 1:50-400 Immunocytochemistry: 1:50-400 (Boiling the paraffin sections in 10mM citrate buffer,pH6.0,or PH8.0 EDTA repair liquid for 20 mins is required for the staining of formalin/paraffin sections.) Optimal working dilutions must be determined by end user.

Storage 12 months from date of receipt，-20℃ as supplied.6 months 2 to 8℃ after reconstitution. Avoid repeated freezing and thawing

Background Information CAMKK2, Calcium/calmodulin-dependent protein kinase kinase 2, is an enzyme that in humans is encoded by the CAMKK2 gene. The product of this gene belongs to the serine/threonine-specific protein kinase family, and to the Ca++/calmodulin-dependent protein kinase subfamily. This protein plays a role in the calcium/calmodulin-dependent(CaM) kinase cascade by phosphorylating the downstream kinases CaMK1 and CaMK4. CaMKK2 regulates production of the appetite stimulating hormone and functions as an AMPK kinase in the . It also has an important role in the development of hyperalgesia and tolerance to analgesic drugs, through reduction in downstream signalling pathways and mu opioid receptor downregulation. Inhibition of CaMKK2 in mice reduces appetite and promotes weight loss.

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FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC AND CLINICAL USE. 1 Product datasheet Anti-CAMKK2 Antibody Catalog Number: BA3629

BOSTER BIOLOGICAL TECHNOLOGY Special NO.1, International Enterprise Center, 2nd Guanshan Road, Wuhan, China

Web: www.boster.com.cn Phone: +86 27 67845390 Fax: +86 27 67845390 Email: [email protected]

Selected Validation Data

Lane 1: Rat Brain Tissue IHC(P): Rat Brain Tissue LysateLane 2: Rat Brain Tissue LysateLane 3: Mouse Brain Tissue LysateLane 4: Mouse Brain Tissue Lysate

Figure 3. Western blot Figure 4. Western blot analysis of CAMKK2 using analysis of CAMKK2 using anti-CAMKK2 antibody anti-CAMKK2 antibody (BA3629). (BA3629). Electrophoresis was Electrophoresis was performed on a 5-20% performed on a 5-20% SDS-PAGE gel at 70V SDS-PAGE gel at 70V (Stacking gel) / 90V (Stacking gel) / 90V (Resolving gel) for 2-3 (Resolving gel) for 2-3 hours. The sample well of hours. The sample well of each lane was loaded each lane was loaded with 50ug of sample with 50ug of sample under reducing under reducing conditions. conditions. Lane 1: human Hela Lane 1: human Raji whole whole cell lysates. cell lysate. After Electrophoresis, After Electrophoresis, proteins were transferred proteins were transferred to a Nitrocellulose to a Nitrocellulose membrane at 150mA for membrane at 150mA for 50-90 minutes. Blocked 50-90 minutes. Blocked the membrane with 5% the membrane with 5% Non-fat Milk/ TBS for 1.5 Non-fat Milk/ TBS for 1.5 hour at RT. The hour at RT. The membrane was incubated membrane was incubated with rabbit anti-CAMKK2 with rabbit anti-CAMKK2 antigen affinity purified antigen affinity purified polyclonal antibody polyclonal antibody (Catalog # BA3629) at 0.5 (Catalog # BA3629) at 0.5

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC AND CLINICAL USE. 2 Product datasheet Anti-CAMKK2 Antibody Catalog Number: BA3629

BOSTER BIOLOGICAL TECHNOLOGY Special NO.1, International Enterprise Center, 2nd Guanshan Road, Wuhan, China

Web: www.boster.com.cn Phone: +86 27 67845390 Fax: +86 27 67845390 Email: [email protected]

μg/mL overnight at 4°C, μg/mL overnight at 4°C, then washed with then washed with TBS-0.1%Tween 3 times TBS-0.1%Tween 3 times with 5 minutes each and with 5 minutes each and probed with a goat anti- probed with a goat anti- rabbit IgG-HRP rabbit IgG-HRP secondary antibody at a secondary antibody at a dilution of 1:10000 for 1.5 dilution of 1:10000 for 1.5 hour at RT. The signal is hour at RT. The signal is developed using an developed using an Enhanced Enhanced Chemiluminescent Chemiluminescent detection (ECL) kit detection (ECL) kit (Catalog # EK1002) with (Catalog # EK1002) with Tanon 5200 system. A Tanon 5200 system. A specific band was specific band was detected for CAMKK2 at detected for CAMKK2 at approximately 65KD. The approximately 65KD. The expected band size for expected band size for CAMKK2 is at 65KD. CAMKK2 is at 65KD.

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC AND CLINICAL USE. 3

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