ISSN (Print) : 0974-6846 Indian Journal of Science and Technology, Vol 11(8), DOI: 10.17485/ijst/2018/v11i8/118940, February 2018 ISSN (Online) : 0974-5645 An Attempt to Clarify the Term Croton-Pattern with Emphasis on Lasiosiphon kraussianus (Meisn.) Burtt. Davy ()

Gamal E. B. El Ghazali Al Rass College of Science and Arts, Qassim University, Saudi Arabia; [email protected], [email protected]

Abstract Objectives: distinctive croton-pattern in Lasiosiphon kraussianus. Methods/Statistical Analysis: Light (LM), scanning electron (SEM) and transmission The presentelectron study microscopic aims to (TEM) establish studies the on nature Lasiosiphon of the finekraussianus structures of the exine which account for the

(Meisn.) Burtt. Davy pollen grainsFindings: were examined and illustratedL. kraussianus in an attempt to elucidate its exine pattern. Previous descriptive terms used for similar exine columellaepattern were (structure) reviewed arewith branched special emphasis and fused on together the terms forming croton-pattern, a reticulum retipilate some distance and reticulum beneath cristatum. the surface. Supra- The exine pattern in was interpreted as having mixed croton-patternApplication/Improvements: and micro-echinate sculpturing. The results The

cristatum,tectal pila areand regularly provided arrangedan accurate in ring-likedescription patterns for Lasiosiphon with narrower kraussianus foveolae.. clarified the confusion and/or misuse encountered in previous works in the terms croton-pattern, retipilate and reticulum Keywords: Croton-Pattern, Lasiosiphon kraussianus, Retipilate, Reticulum Cristatum

1. Introduction The croton-pattern of the exine similar to those found in members of the family Thymelaeaceae occurs Lasiosiphon kraussianus (Meisn.) Burtt Davy (= in some genera of the families: Buxaceae, Callitrichaceae, kraussiana Meisn.) which belongs to the fam- , Euphorbiaceae, Liliaceae and ily Thymelaeaceae, is a perennial suffrutescent, erect to Scrophulariaceae (Table 1). semi-prostrate, with woody rootstock1. It is a poison- Though6 has recognized the existence of a unique ous plant2 with insecticidal3 and antileprotic activities4. exine ornamentation present in a number of families/ Thymelaeaceae is a cosmopolitan family (with the excep- genera, the term croton-pattern was not included in the tion of the Polar Regions), containing 45 genera and sculpturing types outlined in the subsequent work con- about 800 species5. ducted7-11. The exine of the pollen grains of the family The term croton-pattern has been interpreted differ- Thymelaeaceae was described as with a characteristic ently by various authors as retipilate or reticulum crista- croton-pattern5. The term croton-pattern was coined5 and tum. Tables (1-3), showed the families and genera/ was defined as “sexine consists of well-defined, regularly where croton-pattern, retipilate and reticulum crista- arranged excrescences (triangular or +/- circular in cross tum have been reported respectively. It was noticed that section), supported (always?) By a baculate, baculoidate, some of the genera that were reported to exhibit a cro- or spongy layer, and arranged regularly in circles around ton-pattern e.g. Erdmanipolis, Pachysandra (Buxaceae), foveoloid areas delimited by muroid ridges”. Thymelae, Phaleria (Thymelaeaeceae), were also described

*Author for correspondence An Attempt to Clarify the Term Croton-Pattern with Emphasis on Lasiosiphon kraussianus (Meisn.) Burtt. Davy (Thymelaeaceae)

Table 1. Families and genera/species where Croton- Table 2. Families and genera/species where retipilate pattern has been reported to exist1 has been reported to exist Family / species References Family Genus/ species References Sarcococcawallichii In28,29 Amaryllidaceae Galanthuselwesii In42 Buxaceae Pachysandra In28 Annonaceae Annonareticulatum In43 Callitriche In23 In44 Callitrichaceae Bignoniaceae C. antartica 21In Spathodeacampanulata In45 Parashoreatomentella Burseraceae Commiphoraleptophloeos In46 multiflora Callitriche In22 Dipterocarpaceae In30 Callitrichaceae S. leprosula C. obtusangula In19 S. xanthophylla Caprifoliaceae Viburnum lantana In47 Carcianutans 21In Helianthemum In48 Cistaceae Croton In31 Fumanaericoides In49 Monihot 32In Cuscutaceae Cuscutalupuliformis In19 Euphorbiaceae Brasiliocroton 33In Geraniaceae Geranium spp. In 50 Jatropha 34In Stachysyildirimlii In51 Lamiaceae Pausandramorisiana 35In Salvia leucantha In52 Lloydiatibetica Liliaceae Liliumspp. In53 Liliaceae In36 Liliumcanadense Asparagus In54 Bartsia In37 Pedicularisspp. In55 Agalinis Euphrasiaspp. In56 Scrophulariaceae Esterhazya Orthocarpus+ 12 other In25 In57 genera Melasma Orobanchaceae Physocalyx Orobanche In58 Aeginetiaindica+ 5 other Gnidia In26 genera Lachnaea In38 Rhinanthus+ 17 other In59 genera Pimeleaarenaria In22,24 Odontites spp. In60 cneorum Esterhazyasplendida 21 D. laureola In Bartsiainequalis Scrophulariaceae In26 Thymelaeaceae Thymelaeapasserina Agalinismaritima In39,5 A. indica 61 Daphnopsisracemosa In40 Parentucellia In 62 Thymelaeahirsuta In41 Viticoideae Vitex In Thymelaeaspp., Phaleriaspp. + 33 other In20 genera and 2). The exine sculptures in the genera Callitriche (Callitrichaceae), Lilium (Liliaceae) were described by other authors to exhibit a reticulum cristatum orna- with croton-pattern retipilate and reticulum cristatum mentation (Tables 1,3), whereas others e.g. Callitriche (Tables 1-3). Such confusion and/or misuse of the terms (Callitrichaceae), Lilium (Liliaceae) and Bartsia, Agalinis croton- pattern, retipilate and reticulum cristatum neces- and Esterhazy (Scrophylariacae), were recognized to show sitate the need to clarify the nature of these exine orna- croton-pattern and retipilate ornamentation (Tables 1 mentations and provide an accurate description of L.

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Table 3. Families and genera/species where reticulum tion, eyepiece X 10 magnification and phase condenser cristatum has been reported to exis lens (Na = 1.4). LM photography was taken with a Zeiss Family Genus/ species References axiomat. SEM examination was done with Jeol JSM- T200 microscope with 25 kv accelerating voltage and 8mm Amaryllidaceae Hymenocallisspp. In63 working distance. SEM micrographs were made with a Aponogetonaceae Aponogetonmasoalaensis In21 (6X7) Mamiya camera. Material for TEM was fixed in Bombacaceae Pachiraaquatica In64,21 2.5 % glutaraldehyde, treated with veronal acetate-buff- Erdtmanipollis Buxaceae In21 ered osmium tetroxide (1%) for 2 hours, post-stained Pachsandra terminalis with freshly prepared aqueous uranyl acetate, and then Callitrichaceae Callitriche polymorpha In21 embedded in Araldite-Epon resin. After embedding and Cistaceae Fumanaprocumbers 21In polymerization of the resin, sections were cut with glass Cuscutaceae Cuscuta lupuliformis In21 knives on Reichert-utramicrotomes, collected on 200 Euphorbiaceae Mercurialisperennis In21 mesh uncoated copper grids, stained with lead citrate for Pedaliaceae Sesamum indicum In65 5 minutes, washed, dried and examined with Zeiss TEM microscope. Geraniaceae Geranium canariense In21 Lilium martagon Liliaceae In21 L. candidum 3. Results Oleaceae Olea europea In66 3.1 General Characters Thymelaeaceae Phaleriacapitata In21 Thymelaea passerine The pollen grains ofL. kraussianus (Figure 1) are sphe- roidal and periporate. The pores (p) are open, circular, at the bottom of deep lumina, slightly larger than the adja- cent lumina, surrounded by columellae; annuli absent or kraussianus. The aim of the present study is to establish the indistinct. The exine is semi-tectate with the ektexine very nature of the fine structures of the exine which account for much thicker than the undulating endexine (Figure 1 A). the distinctive croton-pattern in L. kraussianus using light microscope (LM), scanning electron microscope (SEM), 3.2 Exine Pattern transmission electron microscope (TEM), and conse- quently to elucidate a type of exine pattern that has been 3.2.1 Structure interpreted differently by various authors. Columellae distinct Figure B,C branched and fused together (Figure 1 D), forming a reticulum some distance 2. Material and Methods beneath the surface (Figure 1 E). reticulum continuous all over the surface, hetero-brochate; muri simpli-columel- Herbarium samples of L. kraussianus (Wickens 2664, late; lumina +/-circular, diameter larger than the width of Jebel Marra, Sudan, KHU) were treated according to the the separating muri. standard acetolysis method for recent pollen grains8,12. The bulk of the sample after chemical treatment and 3.1.2 Sculpturing staining with basic fuchsin was stored in distilled water Mixed-sculpturing; croton-pattern and micro-echinate in small vials. This stock pollen sample was then used (Figure 1 .F, G) Supra-tectal elements (or subunits) exist partly to make voucher slides for LM examination, partly on top of the columellae. These elements (or subunits) are for the preparation of aluminum stubs for SEM examina- Pilate. Pila (clavae) (pi) free from each other (detached tion, and partly for the preparation of capsules for TEM pila), triangular at the distal face of the caput (t), with psi- examination. Voucher slides for LM examination were late or finely granular-striate surface, regularly arranged mounted in 85% glycerol and sealed with paraffin wax. in groups of 5-8 given the appearance of ring-like pat- LM examination was carried out with Zeiss microscope terns (circles) at the surface, beset with minute spines using phase contrast (ph) objective X 1000 magnifica- (spinules). The latter are < 1 µm in height, conical, blunt

Vol 11 (8) | February 2018 | www.indjst.org Indian Journal of Science and Technology 3 An Attempt to Clarify the Term Croton-Pattern with Emphasis on Lasiosiphon kraussianus (Meisn.) Burtt. Davy (Thymelaeaceae)

blance between the Thymelaeaceae and the subfamily Crotonoideae in the exine pattern 6 to propose the term “Croton-pattern”. The latter term, was used to describe species of the above mentioned related families and the unrelated Monocotyledonous species6. The term croton-pattern was encountered to be con- fused with the term reticulum cristatum6,19,20 described the exine of various members of the family Thymelaeaceae as characterized by croton pattern. However in21 described Thymelaea passerine and Phaleria capitate (both belong to the family Thymelaeaceae) as with reticulum cristatum. The latter term was defined21 as a special type of reticulum with muri having a prominent sculpturing elements. As a matter of fact, in L. kraussianus, the columellae (structure) are branched and fused together forming a reticulum some distance beneath the surface. Since the reticulum in L. kraussianus is formed beneath the surface, it is not a surface feature, relief or topography22 and should not be regarded as surface ornamentation. The term croton-pattern was also confused with the term retipilate. The surface ornamentation of various members of the genus Callitriche were regarded6,19,22,23 as with croton-pattern, and were considered22,24 as with retipilate sculpturing. In addition, the surface ornamen- tation of the genera Bartsia, Agalinis and Esterhazya (Scrophullariaceae) were described25 as with croton-pat- tern, whereas26 regarded these genera as with retipilate sculpturing. The latter term, was defined6 as with a retic- Figure 1. Lasiosiphonkraussianus (Thymelaeeaceae) pollen.(A-B), (C-D), (E-F). Light (LM), Scanning (SEM), ulum, whereas the muri consist of detached pila. In L. And Transmission (TEM) micrographs. Bar=5 um. kraussianus, these detached pila are triangular at the dis- tal face and regularly arranged in groups of 5-8 given the appearance of ring-like pattern around foveolate areas. at the apices, usually 1-2, mainly located at the centre of Such pattern of pila, although regular, are not in reticu- the pila (Figure H, I). late pattern sensu27, since the foveolate areas (lumina) are narrower than the delimiting pila, and consequently the 4. Discussion term retipilate is not applicable for the description of the sculpturing of L. kraussianus. 6 Thymelaeaceae is a stenopalynous family , being charac- The term retipilate was regarded21 as a controversial terized mainly by having periporate apertures and similar or fuzzy term and outlined that the diagram illustrated in exine pattern. These two characters together have high their glossary of pollen and spores terminology are erro- taxonomic values giving the family its unique pollen-type. neous. The term reticulum cristatum may be regarded as 13 Periporate apertures are characteristic of some families , the prominent term to be confused with croton-pattern. whereas, the exine pattern is only exhibited by few spe- In the latter pattern, the sculpturing elements are regu- cies scattered amongst few families with the exception larly arranged in ring-like pattern with narrower foveolae 14 of all members of the subfamily Crotonoideae . The (lumina), whereas in reticulum cristatum, the sculpturing 15-17 Thymelacaceae shows a number of similarities to the , elements are in a network reticulum with wider lumina 18 but differs in some floral characteristics . The resem- than the delimiting muri.

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5. Conclusion 12. Erdtman G. The acetolysis method. SvenskBotaniskTidskrift. 1960; 54:561-4. • Using (LM), (SEM) and (TEM), Lasiosiphon- 13. El Ghazali GEB. A study on the pollen flora of Sudan. Review kraussianus was described as having mixed cro- of Palaeobotanty and Palynology. 1993; 76(2-4):99-345. ton-pattern and micro-echinate sculpturing. crossref. • A review was undertaken for the terms reticu- 14. Punt W. A survey of the pollen morphology in Euphorbiaceae lum cristatum and retipilate showing confusing with special reference to Phyllanthus. Botanical Journal of the Linnaean Society. 1987; 94(1-2):127-42. crossref. exine pattern with the term croton-pattern. 15. Paris R. The distribution of glycosides. Chemical • The terms reticulum cristatum and retipilate plant , Swain T (Edition). New York: Academic were regarded inapplicable for the description of Press. 1963; p. 1-337. crossref. the exine pattern in Lasiosiphon kraussianus. 16. Wunderlich R. Some remarks on the taxonomic sig- nificance of the seed-coat. Phytomorphology. 1968; 17: 6. Acknowledgement 301-11. 17. Ourisson G. Some aspects of the distribution of diterpenes I appreciate very much the various help received from in . Chemistry in botanical classification. Benz G, Sayed Yousif El Sheikh, EM unit (University of Khartoum, Santesson J (ed.). New York: Academic Press. 1973; p. 20-5. Sudan) and Jan Berge (Botanical Institute, University of 18. Webster GL. The saga of the spurges: a review of classifi- Bergen, Norway). cation and relationships in the Euphorbiales. Botanical Journal of the Linnaean Society. 1987; 94:3-46. crossref. 19. Erdtman G. Pollen morphology and plant taxonomy 7. Reference (Angiosperms). New York: Hafner Publishing Company. 1986; p. 1-553.

1. Quattrocchi U. CRC world dictionary of medicinal and 20. Herber BE. Pollen morphology of the Thymelaeaceae in poisonous plants. CRC Press: Florida. 2016; p. 185-222. relation to its taxonomy. Plant Systematics and Evolution.

2. Wagstaff DJ. International poisonous plants checklist. An 2002; 232(1-2):107-21. crossref.

evidence-based reference. Florida: CRC Press. 2008; p. 1-462. 21. Hesse M, Halbritter H, Zetter R, Weber M, Buchner R,

crossref. crossref. PMCid:PMC2491492. Frosch-Radivo A, Utrich S. Pollen terminology An illus-

3. Bala AEA, Delorme R, Kollmann A, Kerhoas L, Einhorn trated handbook. Springer Wien New York. 2009; p. 1-261.

J, Ducrot P, Auge D. Insecticidal activity of daphnane PMid:18771459.

diterpenes from Lasiosiphon kraussianus (Meisn.), 22. Punt W, Hoen PP, Blackmore S, Nilsson S, Le Thomas (Thymelaeaceae) roots. 1999; 55(7):745-50. A. Glossary of pollen and spore terminology. Review

4. Barbosa-Filho JM, Junior FAN, Tomaz ACA, Athayde-Filho of Palaeobotany and Palynology. 2007; 143(1-2):1-81.

PF, Cunham EV, Souza MF, Batista LM, Diniz MFF. Natural crossref.

products with antiprotic activity. Revista Brasileirade 23. Martinsson K. The pollen of Swedish Callitriche

Farmacognosial. 2007; 7(1):141-8. crossref. (Callitrichaceae)-trends towards submergence. Grana.

5. Kubitzki K, Bayer C. Herber BE Thymelaeaceae. The 1993; 32(4-5):198-209. crossref.

families and genera of vascular plants, Flowering plants, 24. Moar NT, Wilmshurst MJ, Mc Glone MS. Standardizing Dicotyledons. Springer- Verlag Berlin. 2003; p. 1-8. names applied to pollen and spores in New Zealand

6. Erdtman G. Pollen morphology and plant taxonomy (angio- Quaternary palynology. New Zealand Journal of Botany. sperms). Almquist & Wiksell Stockholm. 1952; p. 1-553. 2011; 49(2):201-29. crossref.

7. Faegri K, Iversen J. Textbook of pollen analysis, 2nd edn. 25. Santos FDAR, Melhem TS. Ornamentation of the croton- Copenhagen: Munksgaard. 1969; p. 1-237. pattern type on pollen grains of BrasilianScrophulariaceae.

8. Faegri K, Iversen J. Textbook of pollen analysis, 3rd Edition. Acta Botanica Malacitana. 2000; 25:81-92. By Faegri K. Copenhagen: Munksgaard. 1975; p. 1-295. 26. Minkin JP, Eshbaugh WH. Pollen morphology of the

9. Faegri K, Iversen J. Textbook of pollen analysis, 4th Edition. Orobanchaceae and rhinanthoidScrophulariaceae. Grana.

By Faegri K, Kaland PE & Krzywinski K. Chichester: John 1989; 28:1-18. crossref. Wiley & Sons. 1989; p. 1-328. 27. Praglowiski J, Punt W. An elucidation of the micro-reticu-

10. Moore PD, Webb JA. An illustrated guide to pollen analysis. late structure of the exine. Grana. 1973; 13:45-50. crossref. London: Hodder & Stoughton. 1978; p. 1-192. 28. Kohler E. Buxaceae In The families and genera of vascu-

11. Hughes NF. Traverse A Paleopalynology Unwin London. lar plants, Flowering plants, Kubitzki K (Edition). 1988; 126(2):205-6. Springer-Verlag Berlin. 2007; p. 1-10.

Vol 11 (8) | February 2018 | www.indjst.org Indian Journal of Science and Technology 5 An Attempt to Clarify the Term Croton-Pattern with Emphasis on Lasiosiphon kraussianus (Meisn.) Burtt. Davy (Thymelaeaceae)

29. Premathilake R, Nilsson S. Pollen morphology of endemic 44. Mitra K. Pollen morphology in Bignoniaceae in relation to species of the Horton Plains National Park, Sri Lanka. taxonomy. Nelumbo (The Bulletin of the Botanical Survey Grana. 2001; 40(6):256-79. crossref. of ). 1968; 10(3-4):319-26. 30. Gardner PC, Maycock CR, Khoo E, Burslem DFRP. The mor- 45. Kailas JG, Ramakrishna H, Seetharam DS. Diversity in syn- phology and exine ornamentation of fresh pollen from four colpate pollen of arborescent taxa in Karimnagar District, dipterocarp species in Sabah. Sandakania. 2012; 18:33-44. Telangana State, India. Research Journal of Pharmaceutical, 31. Carreira LMM, Secco RDS, Barth OM. Pollen mor- Biological and Chemical Sciences. 2016; 7(3):771-6. phology of the lianescent species of the genus Croton 46. Aguilar-Sierra CI, Melhem TSA. Pollen morphology (Euphorbiaceae). 1996; 35(2):74-8. of the tribe Bursereae (Burseraceae) in South America. 32. Vieira LJ, Soares TL, Rossi ML, Alves AAC, Dos Santos RevistaBrasileira de Botanica. 1998; 21(1):17-26. FAR, Souza FVDD. Viability, production and morphology 47. Punt W, Reitsma Tj, Reuvers AAML. The Northwest of pollen grains from different species in the genus Manihot European Pollen Flora, 2, Caprifoliaceae. Review of (Euphorbiaceae). Acta Botanica Brasilica. 2012; 26(2):350- Palaeobotany and Palynology. 1974; 17:5-29. crossref. 6. crossref. 48. Hassan NM. Pollen morphology of the family Cistaceae in 33. Berry PE, Cordeiro I, Wiedenhoeft AC, Vitorino-Cruz MA Egypt and its systematic significance. Journal of Systematics and De Lima LR. Brasiliocroton, a new crotonoid genus of and Evolution. 2011; 49(4):362-71. crossref. Euphorbiaceaes.s. from Eastern Brasil. Systematic Botany. 49. De Rivas CS. Pollen morphology of Spanish Cistaceae. 2005; 30(2):357-65. crossref. Grana. 1979; 18:91-8. crossref. 34. Bahadur B, Murthy GVS, Sujatha M. LM and SEM of 50. Stafford PJ, Blackmore S. The Northwest European pol- Jatropha L. Pollen (Euphorbiacaeae). Journal of Plant len flora 46. Review of Palaeobotany & Palynology. 1991; Science & Research. 2012; 28(1):97-108. 69:49-78. crossref. 35. Sales EO, Barreto CF, Barth OM. Pollen morphology of 51. Dinc M, Ozturk M. Comparative morphological, anatomi- arboreal species of Euphorbiaceaes.l. at Santa Catarina cal, and palynological studies on the genus Stachys L. sect. State, Brazil. Hoehnea. 2011; 38(3):493-8. Ambleia Bentham (Lamiaceae) species in Turkey. Turkish 36. Kosenko VN. Contribution to the pollen morphology and Journal of Botany. 2008; 32:113-21. taxonomy of the Liliaceae. Grana. 1999; 38(1):20-30. crossref. 52. Gupta A, Sharma C. Polymorphism in pollen of Salvia leu- 37. Hedberge O, Ericson B, Grill-Willen A, Hunde S, Kallsten cantha (Lamiaceae). Grana. 1990; 29(4):277-84. crossref. L, Lofgren O, Ruuth T, Ryding O. The yellow-flowered 53. Du Y-P, Wei C, Wang Z-X, Li S, He H-B, Jia G-X. Lilium species of Bartsia (Scrophulariaceae) in Tropical . spp. Pollen in China (Liliaceae): taxonomic and phy- Norwegian Journal of Botany. 1979; 26:1-9. logenetic implications and pollen evolution related to 38. Beyers JBP, Marais EM. Palynological studies of the environmental conditions. PloSONE. 2014; 9(1):91543. Thymelaeaceae of Cape flora. Grana. 1998; 37(4):193-202. crossref. PMid:24498208 PMCid:PMC3909241. crossref. 54. Ozler H, Pehlivan S. Comparison of pollen morphologi- 39. Bredenkamp CL, Van Wyk AE. Palynology of the genus cal structures of some taxa belonging to Asparagus L. and Passerina (Thymelaeaceae): relationships form and func- Fritillaria L. (Liliaceae) from Turkey. Bangladesh Journal of tion. Grana. 1996; 35(6):335-46. crossref. Botany. 2007; 36(2):111-20. 40. Mourelle D, Prieto AR. Pollen and spores from surface 55. Mehrvarz SS, Panah SP, Faghir MB. A palynological samples in the campos region of Uruguay and their paleo- study of the genus Pedicularis (Orobanchaceae) in Iran. ecological implications. Acta Botanica Brasilica. 2016; Willdenowia. 2013; 43:279-85. crossref. 30(3):351-70. crossref. 56. Qaiser M, Perveen A, Siddiqui T. Pollen morphology of the 41. Cornara L, Norghesi B, Caporali W, Casazza G, Roccotiello genus Euphrasia L. (Orobanchaceae) from Pakistan and E, Minuto L. Floral features and reproductive ecology in the Kashmir and its taxonomic significance. Plant Systematics Thymelaeairsute (L.) Endl. Plant Systematics and Evolution. and Evolution. 2013; 300(3):483-92. crossref. 2005; 250(3):157-72. crossref. 57. Lu L, Wang H, Blackmore B, Li DZ, Dong LN. Pollen mor- 42. Sahin NF. Pollen morphology of Galanthuselwesii Hooker phology of the tribe Rhinantheae (Orobanchaceae) and its (Amaryllidaceae). Pakistan Journal of Botany. 2000; systematic significances. Plant Systematics and Evolution. 32(1):5-6. 2007; 268:1-177. crossref. 43. Kailas JG, Ramakrishna H, Prabhaker R. Inaperturate 58. Tsymbalyuk EM, Mosyakin SL. Palynomorphology of spe- pollen diversity in the arborescent taxa of Karimnagar dis- cies of the Orobanche L. subgenus Phelipanche (Pomel) trict, Telangana State, India. Indian Journal of Agricultural Tzvelev (Orobanchaceae) in the flora of Ukraine. Ukrainian Research. 2016; 50(4):374-7. Botanical Journal. 2013; 70(5):600-9. crossref.

6 Vol 11 (8) | February 2018 | www.indjst.org Indian Journal of Science and Technology Gamal E. B. El Ghazali

59. Tsymbalyuk ZM. Comparative palynomorphological 63. Martin J, Raymundez MB, Valles J, Garnatje T, Raimundez investigation of some genera of Orobanchaceae in the E. Palynological study of the Venezuelan species of the flora of Ukraine. Ukrainian Botanical Journal. 2011; genus Hymenocallis (Amaryllidaceae). Plant Systemics and 68(1):600-9. Evolution. 2012; 298:695-701. crossref. 60. Bolliger M, Wick L. The pollen morphology of Odontites 64. De Abreu VHR, Mendonca CBF, Goncalves- Esteves V. (Scrophulariaceae) and its taxonomic significance. Plant Pollen morphology of selected species of the subfam- Systematics and Evolution. 1990; 173(3):159-78. crossref. ily Bombacoideae (Malvaceaesensulato). Acta Botanica 61. Mehrvarz SS, Litehroudi MA, Khanik GB, Shavvon RS. Brasilica. 2014; 28(3):352-60. crossref. Micromorphological, anatomical and palynological studies 65. Akhila H, Beevy SS. Palynological characterization of of the genus ParentucelliaL. (Scrophulariaceae) in Iran. OT species of Sesamum (Pedaliaceae) from Kerala: a system- SistematikBotanikDergisi. 2011; 18(2):57-71. atic approach. Plant Systematics and Evolution. 2015; 62. Cabral C, Moller M and Sales F. The palynological com- 301(9):2179-88. crossref. pass: a case study in Viticoideae (Vitex L.). Microscopy & 66. Najmaddin C. Anatomical and palynological studies of Olea Microanalysis. 2008; 14(3):158-61. crossref. europea. Bangladesh Journal of Botany. 2016; 45(2):321-6.

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