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H2AFV Rabbit pAb

Catalog No.: A17813

Basic Information Background

Catalog No. are basic nuclear that are responsible for the structure of A17813 the chromosomal fiber in . consist of approximately 146 bp of DNA wrapped around a octamer composed of pairs of each of the four core Observed MW histones (H2A, H2B, H3, and H4). The fiber is further compacted through the 14kDa interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This encodes a replication-independent histone Calculated MW that is a member of the family. Several transcript variants encoding different isoforms, have been identified for this gene. [provided by RefSeq, Oct 2015] Category Primary antibody

Applications WB, IHC, IF

Cross-Reactivity Human, Mouse, Rat

Recommended Dilutions Immunogen Information

WB 1:500 - 1:2000 Gene ID Swiss Prot 94239 Q71UI9 IHC 1:50 - 1:200 Immunogen 1:50 - 1:200 IF Recombinant fusion containing a sequence corresponding to amino acids 1-128 of human H2AFV (NP_036544.1).

Synonyms H2A.Z-2;H2AV;H2AFV

Contact Product Information

400-999-6126 Source Isotype Purification Rabbit IgG Affinity purification [email protected]

www.abclonal.com.cn Storage Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3. Validation Data

Western blot analysis of extracts of various cell lines, using H2AFV antibody (A17813) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s.

Immunohistochemistry of paraffin- Immunofluorescence analysis of C6 cells Immunofluorescence analysis of L929 cells embedded Rat testis using H2AFV Rabbit using H2AFV antibody (A17813) at dilution using H2AFV antibody (A17813) at dilution pAb (A17813) at dilution of 1:100 (40x of 1:100. Blue: DAPI for nuclear staining. of 1:100. Blue: DAPI for nuclear staining. lens).

Immunofluorescence analysis of U-2 OS cells using H2AFV antibody (A17813) at dilution of 1:100. Blue: DAPI for nuclear staining.

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